Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity

Infiltration of biomacromolecules into porous silicon photonic architectures results in biofunctionalized structures with unique properties. Characterization of their optical response and performance optimization in biomacromolecular detection and biophotonic application require a combination of opt...

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Veröffentlicht in:Applied physics letters 2009-06, Vol.94 (22)
Hauptverfasser: Martin, M., Palestino, G., Cloitre, T., Agarwal, V., Zimányi, L., Gergely, C.
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container_issue 22
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container_title Applied physics letters
container_volume 94
creator Martin, M.
Palestino, G.
Cloitre, T.
Agarwal, V.
Zimányi, L.
Gergely, C.
description Infiltration of biomacromolecules into porous silicon photonic architectures results in biofunctionalized structures with unique properties. Characterization of their optical response and performance optimization in biomacromolecular detection and biophotonic application require a combination of optical and structural studies. Nonlinear optical microscopy is applied to study porous silicon microcavities with and without infiltrated glucose oxidase. The infiltrated protein acts as an internal two-photon-excited fluorescence emitter and second harmonic generator, enabling the in-depth visualization of the porous structure. Enhanced second harmonic generation and fluorescence emission by the porous silicon structure is experimentally associated with the defect layer.
doi_str_mv 10.1063/1.3148698
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subjects Biological Physics
Physics
title Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity
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