Quantification of zidovudine and its monophosphate in cell extracts by on-line solid-phase extraction coupled to liquid chromatography
A simple and rapid analytical method for the simultaneous quantification of zidovudine (AZT) and its monophosphate (AZTMP) in cell extracts has been developed using high-performance liquid chromatography (HPLC) with on-line solid-phase extraction and 2-aminoethyl-3′-azido-2′,3′-dideoxythymidin-5′-yl...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-10, Vol.858 (1), p.2-7 |
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| creator | Lefebvre, I. Puy, J.-Y. Perrin, C. Périgaud, C. |
| description | A simple and rapid analytical method for the simultaneous quantification of zidovudine (AZT) and its monophosphate (AZTMP) in cell extracts has been developed using high-performance liquid chromatography (HPLC) with on-line solid-phase extraction and 2-aminoethyl-3′-azido-2′,3′-dideoxythymidin-5′-yl phosphodiester sodium salt as internal standard (IS). The cell extract samples were directly injected on a short reversed-phase precolumn using an aqueous buffer containing an ion-pairing reagent as a mobile phase. Under these conditions, the analytes were retained on the precolumn whereas the proteins were discarded. The analytes were then transferred onto the analytical column by increasing the strength of the eluent. The calibration curve was linear over a concentration range of 0.5–100
μg/ml. Inter- and intra-day accuracy and precision results satisfied the accepted criteria for bioanalytical validation. This method was used to study the decomposition pathway of a model pronucleotide in an
in vitro approach. |
| doi_str_mv | 10.1016/j.jchromb.2007.07.028 |
| format | Article |
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μg/ml. Inter- and intra-day accuracy and precision results satisfied the accepted criteria for bioanalytical validation. This method was used to study the decomposition pathway of a model pronucleotide in an
in vitro approach.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2007.07.028</identifier><identifier>PMID: 17870675</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Cell extracts ; Chemical Sciences ; Chromatography, High Pressure Liquid - methods ; Dideoxynucleotides - analysis ; Dideoxynucleotides - chemistry ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Ion pair ; Medical sciences ; Molecular Structure ; On-line solid-phase extraction ; Organic chemistry ; Pharmacology. Drug treatments ; Reproducibility of Results ; Solid Phase Extraction - methods ; Thymine Nucleotides - analysis ; Thymine Nucleotides - chemistry ; Zidovudine ; Zidovudine - analogs & derivatives ; Zidovudine - analysis ; Zidovudine - chemistry ; Zidovudine monophosphate</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-10, Vol.858 (1), p.2-7</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-4d979755c5be6219f9eec559927b62cee1b9d80e0927c05c9c2879898a0071ad3</citedby><cites>FETCH-LOGICAL-c456t-4d979755c5be6219f9eec559927b62cee1b9d80e0927c05c9c2879898a0071ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2007.07.028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19154161$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17870675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00357497$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Lefebvre, I.</creatorcontrib><creatorcontrib>Puy, J.-Y.</creatorcontrib><creatorcontrib>Perrin, C.</creatorcontrib><creatorcontrib>Périgaud, C.</creatorcontrib><title>Quantification of zidovudine and its monophosphate in cell extracts by on-line solid-phase extraction coupled to liquid chromatography</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A simple and rapid analytical method for the simultaneous quantification of zidovudine (AZT) and its monophosphate (AZTMP) in cell extracts has been developed using high-performance liquid chromatography (HPLC) with on-line solid-phase extraction and 2-aminoethyl-3′-azido-2′,3′-dideoxythymidin-5′-yl phosphodiester sodium salt as internal standard (IS). The cell extract samples were directly injected on a short reversed-phase precolumn using an aqueous buffer containing an ion-pairing reagent as a mobile phase. Under these conditions, the analytes were retained on the precolumn whereas the proteins were discarded. The analytes were then transferred onto the analytical column by increasing the strength of the eluent. The calibration curve was linear over a concentration range of 0.5–100
μg/ml. Inter- and intra-day accuracy and precision results satisfied the accepted criteria for bioanalytical validation. This method was used to study the decomposition pathway of a model pronucleotide in an
in vitro approach.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cell extracts</subject><subject>Chemical Sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dideoxynucleotides - analysis</subject><subject>Dideoxynucleotides - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Ion pair</subject><subject>Medical sciences</subject><subject>Molecular Structure</subject><subject>On-line solid-phase extraction</subject><subject>Organic chemistry</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Solid Phase Extraction - methods</subject><subject>Thymine Nucleotides - analysis</subject><subject>Thymine Nucleotides - chemistry</subject><subject>Zidovudine</subject><subject>Zidovudine - analogs & derivatives</subject><subject>Zidovudine - analysis</subject><subject>Zidovudine - chemistry</subject><subject>Zidovudine monophosphate</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVGL1DAQx4so3nn6EZS8KPjQNWk3SfMkx3HnCQsiKPgW0mRqs7RJL0kX1w_g577UrXePwkDCzG_-mcy_KF4TvCGYsA_7zV73wY_tpsKYb5aomifFOWl4Xdac_Xia75TjEld1dVa8iHGPMeGY18-LM8Ibjhmn58Wfr7NyyXZWq2S9Q75Dv63xh9lYB0g5g2yKaPTOT72PU68SIOuQhmFA8CsFpXO5PSLvymHpiH6wpsxchH_1RVb7eRrAoOTRYO9ma9Df4VXyP4Oa-uPL4lmnhgiv1vOi-H5z_e3qttx9-fT56nJX6i1lqdwawQWnVNMWWEVEJwA0pUJUvGWVBiCtMA0GnBMaUy101XDRiEblHRFl6ovi_Um3V4Ocgh1VOEqvrLy93Mklh3FN-VbwA8nsuxM7BX83Q0xytHH5uHLg5yhZU7M6sxmkJ1AHH2OA7kGZYLmYJfdyNUsuZsklqib3vVkfmNsRzGPX6k4G3q6AiloNXVBO2_jICUK3hC2TfjxxkFd3sBBk1BacBmMD6CSNt_8Z5R72Obef</recordid><startdate>20071015</startdate><enddate>20071015</enddate><creator>Lefebvre, I.</creator><creator>Puy, J.-Y.</creator><creator>Perrin, C.</creator><creator>Périgaud, C.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20071015</creationdate><title>Quantification of zidovudine and its monophosphate in cell extracts by on-line solid-phase extraction coupled to liquid chromatography</title><author>Lefebvre, I. ; Puy, J.-Y. ; Perrin, C. ; Périgaud, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-4d979755c5be6219f9eec559927b62cee1b9d80e0927c05c9c2879898a0071ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cell extracts</topic><topic>Chemical Sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dideoxynucleotides - analysis</topic><topic>Dideoxynucleotides - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Ion pair</topic><topic>Medical sciences</topic><topic>Molecular Structure</topic><topic>On-line solid-phase extraction</topic><topic>Organic chemistry</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Solid Phase Extraction - methods</topic><topic>Thymine Nucleotides - analysis</topic><topic>Thymine Nucleotides - chemistry</topic><topic>Zidovudine</topic><topic>Zidovudine - analogs & derivatives</topic><topic>Zidovudine - analysis</topic><topic>Zidovudine - chemistry</topic><topic>Zidovudine monophosphate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lefebvre, I.</creatorcontrib><creatorcontrib>Puy, J.-Y.</creatorcontrib><creatorcontrib>Perrin, C.</creatorcontrib><creatorcontrib>Périgaud, C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of chromatography. 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B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2007-10-15</date><risdate>2007</risdate><volume>858</volume><issue>1</issue><spage>2</spage><epage>7</epage><pages>2-7</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A simple and rapid analytical method for the simultaneous quantification of zidovudine (AZT) and its monophosphate (AZTMP) in cell extracts has been developed using high-performance liquid chromatography (HPLC) with on-line solid-phase extraction and 2-aminoethyl-3′-azido-2′,3′-dideoxythymidin-5′-yl phosphodiester sodium salt as internal standard (IS). The cell extract samples were directly injected on a short reversed-phase precolumn using an aqueous buffer containing an ion-pairing reagent as a mobile phase. Under these conditions, the analytes were retained on the precolumn whereas the proteins were discarded. The analytes were then transferred onto the analytical column by increasing the strength of the eluent. The calibration curve was linear over a concentration range of 0.5–100
μg/ml. Inter- and intra-day accuracy and precision results satisfied the accepted criteria for bioanalytical validation. This method was used to study the decomposition pathway of a model pronucleotide in an
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Cell extracts Chemical Sciences Chromatography, High Pressure Liquid - methods Dideoxynucleotides - analysis Dideoxynucleotides - chemistry Fundamental and applied biological sciences. Psychology General pharmacology Ion pair Medical sciences Molecular Structure On-line solid-phase extraction Organic chemistry Pharmacology. Drug treatments Reproducibility of Results Solid Phase Extraction - methods Thymine Nucleotides - analysis Thymine Nucleotides - chemistry Zidovudine Zidovudine - analogs & derivatives Zidovudine - analysis Zidovudine - chemistry Zidovudine monophosphate |
| title | Quantification of zidovudine and its monophosphate in cell extracts by on-line solid-phase extraction coupled to liquid chromatography |
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