Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006

In order to investigate the genetic heterogeneity of ovine pestivirus isolates in France, 32-clinical samples collected between 1985 and 2006, previously detected positive by indirect immuno-fluorescence for the presence of Border disease virus (BDV), were tested by reverse transcription-polymerase...

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Veröffentlicht in:Veterinary microbiology 2008-07, Vol.130 (1), p.69-79
Hauptverfasser: Dubois, Eric, Russo, Pierre, Prigent, Myriam, Thiéry, Richard
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creator Dubois, Eric
Russo, Pierre
Prigent, Myriam
Thiéry, Richard
description In order to investigate the genetic heterogeneity of ovine pestivirus isolates in France, 32-clinical samples collected between 1985 and 2006, previously detected positive by indirect immuno-fluorescence for the presence of Border disease virus (BDV), were tested by reverse transcription-polymerase chain reaction (RT-PCR). Parts of the 5′-non-coding region (5′-NCR) and the Npro-coding region were successfully amplified from 23 samples. An internal control was designed and used to assess the validity of negative RT-PCR results, which could be explained by RNA degradation during the storage of samples at −20 °C for at least 16 years. A phylogenetic study was performed by using sequences obtained from the two loci. French isolates clustered into four distinct groups. Six isolates were assigned to the previously described BDV-3 group. However, some isolates could not be assigned to any existing phylogenetic BDV groups, and therefore, tentative new phylogenetic groups are suggested. The AV strain, isolated in 1984 from sheep showing a severe hemorrhagic syndrome in the rearing region of Aveyron in France and sequenced during this study, should be considered as the strain model of the BDV-5 group. Nine viral sequences clustered in a set distinct from all other groups were assigned to the BDV-6 group. Two viral sequences were distinct from the BDV phylum and composed the last set assigned to the group of unclassified pestivirus that had been previously isolated in Tunisia. The marked diversity of pestiviruses might reflect the sheep trade in France and with foreign countries.
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Parts of the 5′-non-coding region (5′-NCR) and the Npro-coding region were successfully amplified from 23 samples. An internal control was designed and used to assess the validity of negative RT-PCR results, which could be explained by RNA degradation during the storage of samples at −20 °C for at least 16 years. A phylogenetic study was performed by using sequences obtained from the two loci. French isolates clustered into four distinct groups. Six isolates were assigned to the previously described BDV-3 group. However, some isolates could not be assigned to any existing phylogenetic BDV groups, and therefore, tentative new phylogenetic groups are suggested. The AV strain, isolated in 1984 from sheep showing a severe hemorrhagic syndrome in the rearing region of Aveyron in France and sequenced during this study, should be considered as the strain model of the BDV-5 group. Nine viral sequences clustered in a set distinct from all other groups were assigned to the BDV-6 group. 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subjects Animal biology
Animals
Autoprotease coding sequence
Biological and medical sciences
border disease
Border disease virus
disease prevalence
emerging diseases
epidemiological studies
France
France - epidemiology
Fundamental and applied biological sciences. Psychology
Genetic Variation
genotype
Genotyping
geographical variation
Life Sciences
longitudinal studies
Microbiology
Microbiology and Parasitology
Miscellaneous
molecular epidemiology
molecular genetics
pathogen identification
pathotypes
Pestivirus
Pestivirus - genetics
Pestivirus Infections
Pestivirus Infections - epidemiology
Pestivirus Infections - veterinary
Pestivirus Infections - virology
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Viral
RNA, Viral - isolation & purification
RT-PCR
Sheep
Sheep Diseases
Sheep Diseases - epidemiology
Sheep Diseases - virology
strains
Time Factors
Variation (Genetics)
Veterinary medicine and animal Health
Virology
title Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006
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