Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006
In order to investigate the genetic heterogeneity of ovine pestivirus isolates in France, 32-clinical samples collected between 1985 and 2006, previously detected positive by indirect immuno-fluorescence for the presence of Border disease virus (BDV), were tested by reverse transcription-polymerase...
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description | In order to investigate the genetic heterogeneity of ovine pestivirus isolates in France, 32-clinical samples collected between 1985 and 2006, previously detected positive by indirect immuno-fluorescence for the presence of Border disease virus (BDV), were tested by reverse transcription-polymerase chain reaction (RT-PCR). Parts of the 5′-non-coding region (5′-NCR) and the Npro-coding region were successfully amplified from 23 samples. An internal control was designed and used to assess the validity of negative RT-PCR results, which could be explained by RNA degradation during the storage of samples at −20
°C for at least 16 years. A phylogenetic study was performed by using sequences obtained from the two loci. French isolates clustered into four distinct groups. Six isolates were assigned to the previously described BDV-3 group. However, some isolates could not be assigned to any existing phylogenetic BDV groups, and therefore, tentative new phylogenetic groups are suggested. The AV strain, isolated in 1984 from sheep showing a severe hemorrhagic syndrome in the rearing region of Aveyron in France and sequenced during this study, should be considered as the strain model of the BDV-5 group. Nine viral sequences clustered in a set distinct from all other groups were assigned to the BDV-6 group. Two viral sequences were distinct from the BDV phylum and composed the last set assigned to the group of unclassified pestivirus that had been previously isolated in Tunisia. The marked diversity of pestiviruses might reflect the sheep trade in France and with foreign countries. |
doi_str_mv | 10.1016/j.vetmic.2008.01.002 |
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°C for at least 16 years. A phylogenetic study was performed by using sequences obtained from the two loci. French isolates clustered into four distinct groups. Six isolates were assigned to the previously described BDV-3 group. However, some isolates could not be assigned to any existing phylogenetic BDV groups, and therefore, tentative new phylogenetic groups are suggested. The AV strain, isolated in 1984 from sheep showing a severe hemorrhagic syndrome in the rearing region of Aveyron in France and sequenced during this study, should be considered as the strain model of the BDV-5 group. Nine viral sequences clustered in a set distinct from all other groups were assigned to the BDV-6 group. Two viral sequences were distinct from the BDV phylum and composed the last set assigned to the group of unclassified pestivirus that had been previously isolated in Tunisia. The marked diversity of pestiviruses might reflect the sheep trade in France and with foreign countries.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2008.01.002</identifier><identifier>PMID: 18299176</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal biology ; Animals ; Autoprotease coding sequence ; Biological and medical sciences ; border disease ; Border disease virus ; disease prevalence ; emerging diseases ; epidemiological studies ; France ; France - epidemiology ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; genotype ; Genotyping ; geographical variation ; Life Sciences ; longitudinal studies ; Microbiology ; Microbiology and Parasitology ; Miscellaneous ; molecular epidemiology ; molecular genetics ; pathogen identification ; pathotypes ; Pestivirus ; Pestivirus - genetics ; Pestivirus Infections ; Pestivirus Infections - epidemiology ; Pestivirus Infections - veterinary ; Pestivirus Infections - virology ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; RNA, Viral ; RNA, Viral - isolation & purification ; RT-PCR ; Sheep ; Sheep Diseases ; Sheep Diseases - epidemiology ; Sheep Diseases - virology ; strains ; Time Factors ; Variation (Genetics) ; Veterinary medicine and animal Health ; Virology</subject><ispartof>Veterinary microbiology, 2008-07, Vol.130 (1), p.69-79</ispartof><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-9ef3084732a94a60f4318ff0f7f3f203ab642c5364af4ab7213e1b3bfdf7440a3</citedby><cites>FETCH-LOGICAL-c494t-9ef3084732a94a60f4318ff0f7f3f203ab642c5364af4ab7213e1b3bfdf7440a3</cites><orcidid>0000-0003-1035-2782 ; 0000-0003-2049-918X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2008.01.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20476225$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18299176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://anses.hal.science/hal-00344018$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Dubois, Eric</creatorcontrib><creatorcontrib>Russo, Pierre</creatorcontrib><creatorcontrib>Prigent, Myriam</creatorcontrib><creatorcontrib>Thiéry, Richard</creatorcontrib><title>Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>In order to investigate the genetic heterogeneity of ovine pestivirus isolates in France, 32-clinical samples collected between 1985 and 2006, previously detected positive by indirect immuno-fluorescence for the presence of Border disease virus (BDV), were tested by reverse transcription-polymerase chain reaction (RT-PCR). Parts of the 5′-non-coding region (5′-NCR) and the Npro-coding region were successfully amplified from 23 samples. An internal control was designed and used to assess the validity of negative RT-PCR results, which could be explained by RNA degradation during the storage of samples at −20
°C for at least 16 years. A phylogenetic study was performed by using sequences obtained from the two loci. French isolates clustered into four distinct groups. Six isolates were assigned to the previously described BDV-3 group. However, some isolates could not be assigned to any existing phylogenetic BDV groups, and therefore, tentative new phylogenetic groups are suggested. The AV strain, isolated in 1984 from sheep showing a severe hemorrhagic syndrome in the rearing region of Aveyron in France and sequenced during this study, should be considered as the strain model of the BDV-5 group. Nine viral sequences clustered in a set distinct from all other groups were assigned to the BDV-6 group. Two viral sequences were distinct from the BDV phylum and composed the last set assigned to the group of unclassified pestivirus that had been previously isolated in Tunisia. The marked diversity of pestiviruses might reflect the sheep trade in France and with foreign countries.</description><subject>Animal biology</subject><subject>Animals</subject><subject>Autoprotease coding sequence</subject><subject>Biological and medical sciences</subject><subject>border disease</subject><subject>Border disease virus</subject><subject>disease prevalence</subject><subject>emerging diseases</subject><subject>epidemiological studies</subject><subject>France</subject><subject>France - epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>genotype</subject><subject>Genotyping</subject><subject>geographical variation</subject><subject>Life Sciences</subject><subject>longitudinal studies</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Miscellaneous</subject><subject>molecular epidemiology</subject><subject>molecular genetics</subject><subject>pathogen identification</subject><subject>pathotypes</subject><subject>Pestivirus</subject><subject>Pestivirus - genetics</subject><subject>Pestivirus Infections</subject><subject>Pestivirus Infections - epidemiology</subject><subject>Pestivirus Infections - veterinary</subject><subject>Pestivirus Infections - virology</subject><subject>Phylogeny</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>RNA, Viral</subject><subject>RNA, Viral - isolation & purification</subject><subject>RT-PCR</subject><subject>Sheep</subject><subject>Sheep Diseases</subject><subject>Sheep Diseases - epidemiology</subject><subject>Sheep Diseases - virology</subject><subject>strains</subject><subject>Time Factors</subject><subject>Variation (Genetics)</subject><subject>Veterinary medicine and animal Health</subject><subject>Virology</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90U2LFDEQBuBGFHd29R-I5uKCYLeVj_66CMvi7goDHnTBW6hOV9wMPZ0x6WnRX2-aHtabp0B4qlL1JstecSg48OrDrphp2jtTCICmAF4AiCfZhje1zEWpxNNsA7Jucs5leZadx7gDANVW8Dw7441oW15Xm-z7LY00OcPMAwY0EwX3ByfnR-Yt87MbiR0oTm524RgpMhf9gBP1zI3sJuBo6D3raPpFNDLeNiXDsWdpoupF9sziEOnl6bzI7m8-fbu-y7dfbj9fX21zo1o15S1ZCY2qpcBWYQVWSd5YC7a20gqQ2FVKmFJWCq3CrhZcEu9kZ3tbKwUoL7J3a98HHPQhuD2G39qj03dXW73cAcgEeTPzZC9Xewj-5zGtpfcuGhoGHMkfo65aIQQImaBaoQk-xkD2sTMHvaSvd3pNXy_pa-DpGZHKXp_6H7s99f-KTnEn8PYEMBoc7BKgi49OgKorIcrk3qzOotf4IyRz_1UAlwAt1FW5iI-roJTt7CjoaByl7-hdIDPp3rv_z_oXxAWrfw</recordid><startdate>20080727</startdate><enddate>20080727</enddate><creator>Dubois, Eric</creator><creator>Russo, Pierre</creator><creator>Prigent, Myriam</creator><creator>Thiéry, Richard</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-1035-2782</orcidid><orcidid>https://orcid.org/0000-0003-2049-918X</orcidid></search><sort><creationdate>20080727</creationdate><title>Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006</title><author>Dubois, Eric ; Russo, Pierre ; Prigent, Myriam ; Thiéry, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-9ef3084732a94a60f4318ff0f7f3f203ab642c5364af4ab7213e1b3bfdf7440a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animal biology</topic><topic>Animals</topic><topic>Autoprotease coding sequence</topic><topic>Biological and medical sciences</topic><topic>border disease</topic><topic>Border disease virus</topic><topic>disease prevalence</topic><topic>emerging diseases</topic><topic>epidemiological studies</topic><topic>France</topic><topic>France - epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>genotype</topic><topic>Genotyping</topic><topic>geographical variation</topic><topic>Life Sciences</topic><topic>longitudinal studies</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Miscellaneous</topic><topic>molecular epidemiology</topic><topic>molecular genetics</topic><topic>pathogen identification</topic><topic>pathotypes</topic><topic>Pestivirus</topic><topic>Pestivirus - genetics</topic><topic>Pestivirus Infections</topic><topic>Pestivirus Infections - epidemiology</topic><topic>Pestivirus Infections - veterinary</topic><topic>Pestivirus Infections - virology</topic><topic>Phylogeny</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>RNA, Viral</topic><topic>RNA, Viral - isolation & purification</topic><topic>RT-PCR</topic><topic>Sheep</topic><topic>Sheep Diseases</topic><topic>Sheep Diseases - epidemiology</topic><topic>Sheep Diseases - virology</topic><topic>strains</topic><topic>Time Factors</topic><topic>Variation (Genetics)</topic><topic>Veterinary medicine and animal Health</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dubois, Eric</creatorcontrib><creatorcontrib>Russo, Pierre</creatorcontrib><creatorcontrib>Prigent, Myriam</creatorcontrib><creatorcontrib>Thiéry, Richard</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dubois, Eric</au><au>Russo, Pierre</au><au>Prigent, Myriam</au><au>Thiéry, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2008-07-27</date><risdate>2008</risdate><volume>130</volume><issue>1</issue><spage>69</spage><epage>79</epage><pages>69-79</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>In order to investigate the genetic heterogeneity of ovine pestivirus isolates in France, 32-clinical samples collected between 1985 and 2006, previously detected positive by indirect immuno-fluorescence for the presence of Border disease virus (BDV), were tested by reverse transcription-polymerase chain reaction (RT-PCR). Parts of the 5′-non-coding region (5′-NCR) and the Npro-coding region were successfully amplified from 23 samples. An internal control was designed and used to assess the validity of negative RT-PCR results, which could be explained by RNA degradation during the storage of samples at −20
°C for at least 16 years. A phylogenetic study was performed by using sequences obtained from the two loci. French isolates clustered into four distinct groups. Six isolates were assigned to the previously described BDV-3 group. However, some isolates could not be assigned to any existing phylogenetic BDV groups, and therefore, tentative new phylogenetic groups are suggested. The AV strain, isolated in 1984 from sheep showing a severe hemorrhagic syndrome in the rearing region of Aveyron in France and sequenced during this study, should be considered as the strain model of the BDV-5 group. Nine viral sequences clustered in a set distinct from all other groups were assigned to the BDV-6 group. Two viral sequences were distinct from the BDV phylum and composed the last set assigned to the group of unclassified pestivirus that had been previously isolated in Tunisia. The marked diversity of pestiviruses might reflect the sheep trade in France and with foreign countries.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18299176</pmid><doi>10.1016/j.vetmic.2008.01.002</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-1035-2782</orcidid><orcidid>https://orcid.org/0000-0003-2049-918X</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animal biology Animals Autoprotease coding sequence Biological and medical sciences border disease Border disease virus disease prevalence emerging diseases epidemiological studies France France - epidemiology Fundamental and applied biological sciences. Psychology Genetic Variation genotype Genotyping geographical variation Life Sciences longitudinal studies Microbiology Microbiology and Parasitology Miscellaneous molecular epidemiology molecular genetics pathogen identification pathotypes Pestivirus Pestivirus - genetics Pestivirus Infections Pestivirus Infections - epidemiology Pestivirus Infections - veterinary Pestivirus Infections - virology Phylogeny Reverse Transcriptase Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Viral RNA, Viral - isolation & purification RT-PCR Sheep Sheep Diseases Sheep Diseases - epidemiology Sheep Diseases - virology strains Time Factors Variation (Genetics) Veterinary medicine and animal Health Virology |
title | Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006 |
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