Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion
This paper concerns a new method of characterizing chitosans with different degrees of acetylation ( DA = 2, 11.5 and 21%); it consists of steric exclusion chromatography using a multidetection instrument allowing determination of M, [η] and R G for each point of the chromatograms. From these data,...
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| Veröffentlicht in: | International journal of biological macromolecules 1993-10, Vol.15 (5), p.281-285 |
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| container_title | International journal of biological macromolecules |
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| creator | Rinaudo, Marguerite Milas, Michel Dung, Pham Le |
| description | This paper concerns a new method of characterizing chitosans with different degrees of acetylation (
DA = 2, 11.5 and 21%); it consists of steric exclusion chromatography using a multidetection instrument allowing determination of
M, [η] and
R
G
for each point of the chromatograms. From these data, the Mark-Houwink parameters for intrinsic viscosity and the exponent relating
R
G
and
M are determined. In the same way, the role of the solvent is discussed and the solvent AcOH 0.3
m/AcONa 0.2
m is proposed to reduce the presence of aggregates which perturbs static molecular weight determination by light scattering. The role of the ionic concentration and of the degree of acetylation on the stiffness of chitosan is then discussed and a theoretical treatment is proposed allowing the determination of the intrinsic persistence length (
L
p
= 50
A
̊
) and the prediction of the dimensions in solution (
R
G
and [η]).
L
p
is found to be independent of DA in the range tested. |
| doi_str_mv | 10.1016/0141-8130(93)90027-J |
| format | Article |
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DA = 2, 11.5 and 21%); it consists of steric exclusion chromatography using a multidetection instrument allowing determination of
M, [η] and
R
G
for each point of the chromatograms. From these data, the Mark-Houwink parameters for intrinsic viscosity and the exponent relating
R
G
and
M are determined. In the same way, the role of the solvent is discussed and the solvent AcOH 0.3
m/AcONa 0.2
m is proposed to reduce the presence of aggregates which perturbs static molecular weight determination by light scattering. The role of the ionic concentration and of the degree of acetylation on the stiffness of chitosan is then discussed and a theoretical treatment is proposed allowing the determination of the intrinsic persistence length (
L
p
= 50
A
̊
) and the prediction of the dimensions in solution (
R
G
and [η]).
L
p
is found to be independent of DA in the range tested.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/0141-8130(93)90027-J</identifier><identifier>PMID: 8251442</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetylation ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Carbohydrates ; Chemical Phenomena ; Chemistry, Physical ; Chitin - analogs & derivatives ; Chitin - chemistry ; Chitosan ; degree of acetylation ; Fundamental and applied biological sciences. Psychology ; Holosides ; ionic strength ; Molecular Weight ; Osmolar Concentration ; Other biological molecules ; Solubility ; Viscosity</subject><ispartof>International journal of biological macromolecules, 1993-10, Vol.15 (5), p.281-285</ispartof><rights>1993</rights><rights>1994 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-15d5c95bb2d920f43b02188852cbc2f6daafd744fe75a405a42168fa25fb43443</citedby><cites>FETCH-LOGICAL-c486t-15d5c95bb2d920f43b02188852cbc2f6daafd744fe75a405a42168fa25fb43443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0141-8130(93)90027-J$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4101279$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8251442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00310600$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rinaudo, Marguerite</creatorcontrib><creatorcontrib>Milas, Michel</creatorcontrib><creatorcontrib>Dung, Pham Le</creatorcontrib><title>Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>This paper concerns a new method of characterizing chitosans with different degrees of acetylation (
DA = 2, 11.5 and 21%); it consists of steric exclusion chromatography using a multidetection instrument allowing determination of
M, [η] and
R
G
for each point of the chromatograms. From these data, the Mark-Houwink parameters for intrinsic viscosity and the exponent relating
R
G
and
M are determined. In the same way, the role of the solvent is discussed and the solvent AcOH 0.3
m/AcONa 0.2
m is proposed to reduce the presence of aggregates which perturbs static molecular weight determination by light scattering. The role of the ionic concentration and of the degree of acetylation on the stiffness of chitosan is then discussed and a theoretical treatment is proposed allowing the determination of the intrinsic persistence length (
L
p
= 50
A
̊
) and the prediction of the dimensions in solution (
R
G
and [η]).
L
p
is found to be independent of DA in the range tested.</description><subject>Acetylation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - chemistry</subject><subject>Chitosan</subject><subject>degree of acetylation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Holosides</subject><subject>ionic strength</subject><subject>Molecular Weight</subject><subject>Osmolar Concentration</subject><subject>Other biological molecules</subject><subject>Solubility</subject><subject>Viscosity</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFv1DAQhS0EKtvCPwApB4ToIcV27MS5IFUrSlutxAXO1sQed42yzmJ7K8qvx-lGe-RgWXrvm6fRG0LeMXrFKGs_UyZYrVhDP_XNZU8p7-r7F2TFVNfXlNLmJVmdkNfkPKVfRW0lU2fkTHHJhOAr4tZbiGAyRv8Xsp9CNbnKbH2eEoSr6i648YDB4CwX15sq5YjhIW8rCLay-BDx2QSD-WlcIkKJAB8q_LOHkIr0hrxyMCZ8u_wX5OfN1x_r23rz_dvd-npTG6HaXDNppenlMHDbc-pEM1DOlFKSm8Fw11oAZzshHHYSBC2Ps1Y54NINohGiuSCXx9wtjHof_Q7ik57A69vrjZ610gujLaWPrLAfj-w-Tr8PmLLe-WRwHCHgdEi6a6nqZMcLKI6giVNKEd0pmVE9n0LPPeu5Z903-vkU-r6MvV_yD8MO7Wlo6b74HxYfkoHRRQjGpxMmSjLv-oJ9OWJYenv0GHUyfj6J9RFN1nby_9_jH1F4pG0</recordid><startdate>19931001</startdate><enddate>19931001</enddate><creator>Rinaudo, Marguerite</creator><creator>Milas, Michel</creator><creator>Dung, Pham Le</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>19931001</creationdate><title>Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion</title><author>Rinaudo, Marguerite ; Milas, Michel ; Dung, Pham Le</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-15d5c95bb2d920f43b02188852cbc2f6daafd744fe75a405a42168fa25fb43443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetylation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - chemistry</topic><topic>Chitosan</topic><topic>degree of acetylation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Holosides</topic><topic>ionic strength</topic><topic>Molecular Weight</topic><topic>Osmolar Concentration</topic><topic>Other biological molecules</topic><topic>Solubility</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rinaudo, Marguerite</creatorcontrib><creatorcontrib>Milas, Michel</creatorcontrib><creatorcontrib>Dung, Pham Le</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rinaudo, Marguerite</au><au>Milas, Michel</au><au>Dung, Pham Le</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>1993-10-01</date><risdate>1993</risdate><volume>15</volume><issue>5</issue><spage>281</spage><epage>285</epage><pages>281-285</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>This paper concerns a new method of characterizing chitosans with different degrees of acetylation (
DA = 2, 11.5 and 21%); it consists of steric exclusion chromatography using a multidetection instrument allowing determination of
M, [η] and
R
G
for each point of the chromatograms. From these data, the Mark-Houwink parameters for intrinsic viscosity and the exponent relating
R
G
and
M are determined. In the same way, the role of the solvent is discussed and the solvent AcOH 0.3
m/AcONa 0.2
m is proposed to reduce the presence of aggregates which perturbs static molecular weight determination by light scattering. The role of the ionic concentration and of the degree of acetylation on the stiffness of chitosan is then discussed and a theoretical treatment is proposed allowing the determination of the intrinsic persistence length (
L
p
= 50
A
̊
) and the prediction of the dimensions in solution (
R
G
and [η]).
L
p
is found to be independent of DA in the range tested.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8251442</pmid><doi>10.1016/0141-8130(93)90027-J</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-8130 |
| ispartof | International journal of biological macromolecules, 1993-10, Vol.15 (5), p.281-285 |
| issn | 0141-8130 1879-0003 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00310600v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetylation Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrates Chemical Phenomena Chemistry, Physical Chitin - analogs & derivatives Chitin - chemistry Chitosan degree of acetylation Fundamental and applied biological sciences. Psychology Holosides ionic strength Molecular Weight Osmolar Concentration Other biological molecules Solubility Viscosity |
| title | Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion |
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