Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to l-fucose, l-galactose and d-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calo...
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| Veröffentlicht in: | FEBS letters 2006-02, Vol.580 (3), p.982-987 |
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| creator | Sabin, Charles Mitchell, Edward P. Pokorná, Martina Gautier, Catherine Utille, Jean-Pierre Wimmerová, Michaela Imberty, Anne |
| description | The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to l-fucose, l-galactose and d-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with l-galactose and Met-β-d-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity. |
| doi_str_mv | 10.1016/j.febslet.2006.01.030 |
| format | Article |
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Lectin not only displays an unusually high affinity for fucose but also binds to l-fucose, l-galactose and d-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with l-galactose and Met-β-d-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2006.01.030</identifier><identifier>PMID: 16438968</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adhesins, Bacterial - chemistry ; Adhesins, Bacterial - metabolism ; Ara ; Arabinose ; Binding Sites - physiology ; Biofilms - growth & development ; Crystal structure ; Cystic fibrosis ; d-arabinose ; d-mannose ; Fuc ; Galactose ; l-fucose ; l-Gal ; l-galactose ; Lectin ; lectin II from Pseudomonas aeruginosa ; Lectins - chemistry ; Lectins - metabolism ; Man ; Me-α-Fuc ; Me-α-l-Gal ; Me-α-Man ; Me-β-Ara ; Monosaccharides - chemistry ; Monosaccharides - metabolism ; PA-IIL ; Protein Binding - physiology ; Protein Structure, Tertiary ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - chemistry ; Pseudomonas aeruginosa - physiology ; Thermodynamics ; α-methyl-d-mannopyranoside ; α-methyl-l-fucopyranoside ; α-methyl-l-galactopyranoside ; β-methyl-d-arabinopyranoside</subject><ispartof>FEBS letters, 2006-02, Vol.580 (3), p.982-987</ispartof><rights>2006 Federation of European Biochemical Societies</rights><rights>FEBS Letters 580 (2006) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4936-1f75e46503cf679bcda28659a71eb0f6339849a2052e8172ef0bff24ed97bf1d3</citedby><cites>FETCH-LOGICAL-c4936-1f75e46503cf679bcda28659a71eb0f6339849a2052e8172ef0bff24ed97bf1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2006.01.030$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2006.01.030$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16438968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00305826$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabin, Charles</creatorcontrib><creatorcontrib>Mitchell, Edward P.</creatorcontrib><creatorcontrib>Pokorná, Martina</creatorcontrib><creatorcontrib>Gautier, Catherine</creatorcontrib><creatorcontrib>Utille, Jean-Pierre</creatorcontrib><creatorcontrib>Wimmerová, Michaela</creatorcontrib><creatorcontrib>Imberty, Anne</creatorcontrib><title>Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to l-fucose, l-galactose and d-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with l-galactose and Met-β-d-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.</description><subject>Adhesins, Bacterial - chemistry</subject><subject>Adhesins, Bacterial - metabolism</subject><subject>Ara</subject><subject>Arabinose</subject><subject>Binding Sites - physiology</subject><subject>Biofilms - growth & development</subject><subject>Crystal structure</subject><subject>Cystic fibrosis</subject><subject>d-arabinose</subject><subject>d-mannose</subject><subject>Fuc</subject><subject>Galactose</subject><subject>l-fucose</subject><subject>l-Gal</subject><subject>l-galactose</subject><subject>Lectin</subject><subject>lectin II from Pseudomonas aeruginosa</subject><subject>Lectins - chemistry</subject><subject>Lectins - metabolism</subject><subject>Man</subject><subject>Me-α-Fuc</subject><subject>Me-α-l-Gal</subject><subject>Me-α-Man</subject><subject>Me-β-Ara</subject><subject>Monosaccharides - chemistry</subject><subject>Monosaccharides - metabolism</subject><subject>PA-IIL</subject><subject>Protein Binding - physiology</subject><subject>Protein Structure, Tertiary</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - chemistry</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>Thermodynamics</subject><subject>α-methyl-d-mannopyranoside</subject><subject>α-methyl-l-fucopyranoside</subject><subject>α-methyl-l-galactopyranoside</subject><subject>β-methyl-d-arabinopyranoside</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNklFr2zAUhcXYWLNsP2FDT4M-OJMsW7b2MtLSLoHACutgb0KWrhoF2-oku8W_YH97MgntY_ckJL5zdbjnIPSRkhUllH85rCw0sYVhlRPCV4SuCCOv0ILWFctYwevXaEEILbKyEuwMvYvxQNK9puItOqO8YLXg9QL9vXC9cf0d9hYbZy0E6Afc-d5HpfVeBWcg4mbCLejB9fhmnW23O2yD7_BNhNH4xKqIFYTxzs2qr_h2D6HzZupV53TERg0Kax8CtGoAgx_dsMe_s6AmHIcw6mEMEN-jN1a1ET6cziX6dX11e7nJdj--by_Xu0wXgvGM2qqEgpeEacsr0Wij8pqXQlUUGmI5Y6IuhMpJmUNNqxwsaazNCzCiaiw1bInOj3P3qpX3wXUqTNIrJzfrnZzfSFpjWef8gSb285G9D_7PCHGQnYsa2lb14McoK1LlohT1iyAVFeGMFgksj6AOPsYA9skCJXKOVR7kKVY5xyoJlclP0n06fTA2HZhn1SnHBGyOwKNrYfq_qfL66iL_OXdkrgjhqR6zzSX6dhwFKYYHB0FG7aDXYFxIHZDGuxfc_gP9CMzR</recordid><startdate>20060206</startdate><enddate>20060206</enddate><creator>Sabin, Charles</creator><creator>Mitchell, Edward P.</creator><creator>Pokorná, Martina</creator><creator>Gautier, Catherine</creator><creator>Utille, Jean-Pierre</creator><creator>Wimmerová, Michaela</creator><creator>Imberty, Anne</creator><general>Elsevier B.V</general><general>Wiley</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20060206</creationdate><title>Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures</title><author>Sabin, Charles ; Mitchell, Edward P. ; Pokorná, Martina ; Gautier, Catherine ; Utille, Jean-Pierre ; Wimmerová, Michaela ; Imberty, Anne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4936-1f75e46503cf679bcda28659a71eb0f6339849a2052e8172ef0bff24ed97bf1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adhesins, Bacterial - chemistry</topic><topic>Adhesins, Bacterial - metabolism</topic><topic>Ara</topic><topic>Arabinose</topic><topic>Binding Sites - physiology</topic><topic>Biofilms - growth & development</topic><topic>Crystal structure</topic><topic>Cystic fibrosis</topic><topic>d-arabinose</topic><topic>d-mannose</topic><topic>Fuc</topic><topic>Galactose</topic><topic>l-fucose</topic><topic>l-Gal</topic><topic>l-galactose</topic><topic>Lectin</topic><topic>lectin II from Pseudomonas aeruginosa</topic><topic>Lectins - chemistry</topic><topic>Lectins - metabolism</topic><topic>Man</topic><topic>Me-α-Fuc</topic><topic>Me-α-l-Gal</topic><topic>Me-α-Man</topic><topic>Me-β-Ara</topic><topic>Monosaccharides - chemistry</topic><topic>Monosaccharides - metabolism</topic><topic>PA-IIL</topic><topic>Protein Binding - physiology</topic><topic>Protein Structure, Tertiary</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - chemistry</topic><topic>Pseudomonas aeruginosa - physiology</topic><topic>Thermodynamics</topic><topic>α-methyl-d-mannopyranoside</topic><topic>α-methyl-l-fucopyranoside</topic><topic>α-methyl-l-galactopyranoside</topic><topic>β-methyl-d-arabinopyranoside</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabin, Charles</creatorcontrib><creatorcontrib>Mitchell, Edward P.</creatorcontrib><creatorcontrib>Pokorná, Martina</creatorcontrib><creatorcontrib>Gautier, Catherine</creatorcontrib><creatorcontrib>Utille, Jean-Pierre</creatorcontrib><creatorcontrib>Wimmerová, Michaela</creatorcontrib><creatorcontrib>Imberty, Anne</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabin, Charles</au><au>Mitchell, Edward P.</au><au>Pokorná, Martina</au><au>Gautier, Catherine</au><au>Utille, Jean-Pierre</au><au>Wimmerová, Michaela</au><au>Imberty, Anne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2006-02-06</date><risdate>2006</risdate><volume>580</volume><issue>3</issue><spage>982</spage><epage>987</epage><pages>982-987</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to l-fucose, l-galactose and d-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with l-galactose and Met-β-d-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>16438968</pmid><doi>10.1016/j.febslet.2006.01.030</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2006-02, Vol.580 (3), p.982-987 |
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| source | MEDLINE; Wiley Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Adhesins, Bacterial - chemistry Adhesins, Bacterial - metabolism Ara Arabinose Binding Sites - physiology Biofilms - growth & development Crystal structure Cystic fibrosis d-arabinose d-mannose Fuc Galactose l-fucose l-Gal l-galactose Lectin lectin II from Pseudomonas aeruginosa Lectins - chemistry Lectins - metabolism Man Me-α-Fuc Me-α-l-Gal Me-α-Man Me-β-Ara Monosaccharides - chemistry Monosaccharides - metabolism PA-IIL Protein Binding - physiology Protein Structure, Tertiary Pseudomonas aeruginosa Pseudomonas aeruginosa - chemistry Pseudomonas aeruginosa - physiology Thermodynamics α-methyl-d-mannopyranoside α-methyl-l-fucopyranoside α-methyl-l-galactopyranoside β-methyl-d-arabinopyranoside |
| title | Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: Thermodynamics data correlated with X-ray structures |
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