Sensitive Detection of Selenoproteins in Gel Electrophoresis by High Repetition Rate Femtosecond Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

A laser ablation-ICPMS method using an infrared (1030 nm), low-energy (39 μJ/pulse), high repetition rate (10 kHz), femtosecond laser was developed to improve the sensitivity of detection of heteroatom-containing proteins in 1D polyacrylamide gels. A 2-mm-wide lane was ablated by ultrafast (10 cm s-...

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Veröffentlicht in:	Analytical chemistry (Washington) 2007-09, Vol.79 (17), p.6874-6880
Hauptverfasser:	Ballihaut, Guillaume, Claverie, Fanny, Pécheyran, Christophe, Mounicou, Sandra, Grimaud, Régis, Lobinski, Ryszard
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemical Sciences Desulfococcus multivorans E coli Electrophoresis, Polyacrylamide Gel - methods Epsilonproteobacteria - genetics Epsilonproteobacteria - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Lasers Mass spectrometry Mass Spectrometry - methods Organic chemicals Proteins Reproducibility of Results Selenoproteins - chemistry Selenoproteins - isolation & purification Selenoproteins - metabolism Sensitivity and Specificity Time Factors
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