Identification of phytochelatin-related peptides in maize seedlings exposed to cadmium and obtained enzymatically in vitro
An analytical strategy based on the sensitivity of electrospray tandem mass spectrometry following a simplified and reproducible sample preparation procedure was evaluated for the determination of Cd-induced phytochelatins (PC) and related peptides in four maize varieties. In addition to the three k...
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| Veröffentlicht in: | Phytochemistry (Oxford) 2001-04, Vol.56 (7), p.657-668 |
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| creator | Chassaigne, Hubert Vacchina, Véronique Kutchan, Toni M Zenk, Meinhart H |
| description | An analytical strategy based on the sensitivity of electrospray tandem mass spectrometry following a simplified and reproducible sample preparation procedure was evaluated for the determination of Cd-induced phytochelatins (PC) and related peptides in four maize varieties. In addition to the three known families of PC (PC, desGly-PC and iso-PC(Glu)) that were observed, novel PC and desGly-PC homologues lacking the N-terminal γ-linked Glu were isolated from maize root extracts for the first time. Additionally the complete sequence of iso-PC
3(Glu) was determined by tandem mass spectrometry. Peptides obtained in vivo and in vitro as the result of the reaction of glutathione with the enzyme phytochelatin synthase were compared. Minor forms detected from in vitro reactions include compounds with intramolecular or intermolecular disulfide bonds resulting from the oxidation of SH groups, phytochelatin homologues lacking the N-terminal γ-linked Glu, and new PC-related peptides with a Cys–Cys motif. Since peptides lacking a γGlu residue could be generated as artifacts in electrospray mass spectrometry, the application of capillary electrophoresis with online electrospray mass spectrometry allowed the separation and detection of such peptides as endogenous molecules present in planta and as products of in vitro reactions. |
| doi_str_mv | 10.1016/S0031-9422(00)00489-1 |
| format | Article |
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3(Glu) was determined by tandem mass spectrometry. Peptides obtained in vivo and in vitro as the result of the reaction of glutathione with the enzyme phytochelatin synthase were compared. Minor forms detected from in vitro reactions include compounds with intramolecular or intermolecular disulfide bonds resulting from the oxidation of SH groups, phytochelatin homologues lacking the N-terminal γ-linked Glu, and new PC-related peptides with a Cys–Cys motif. Since peptides lacking a γGlu residue could be generated as artifacts in electrospray mass spectrometry, the application of capillary electrophoresis with online electrospray mass spectrometry allowed the separation and detection of such peptides as endogenous molecules present in planta and as products of in vitro reactions.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/S0031-9422(00)00489-1</identifier><identifier>PMID: 11314950</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Aminoacyltransferases - metabolism ; Analytical chemistry ; Analytical, structural and metabolic biochemistry ; Binding and carrier proteins ; Biological and medical sciences ; Cadmium ; Cadmium - pharmacology ; Cell biochemistry ; Cell physiology ; Chemical Sciences ; Disulfides - analysis ; Electrospray mass spectrometry ; Fundamental and applied biological sciences. Psychology ; Glutathione - metabolism ; Maize seedlings ; Metalloproteins - biosynthesis ; Metalloproteins - chemistry ; Metalloproteins - isolation & purification ; Phytochelatin-related peptides ; Phytochelatins ; Plant physiology and development ; Plant Proteins - biosynthesis ; Plant Proteins - chemistry ; Plant Proteins - isolation & purification ; Proteins ; Spectrometry, Mass, Electrospray Ionization ; Zea mays ; Zea mays - drug effects ; Zea mays - metabolism</subject><ispartof>Phytochemistry (Oxford), 2001-04, Vol.56 (7), p.657-668</ispartof><rights>2001 Elsevier Science Ltd</rights><rights>2001 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-b2ec38b0543de5306738b103f647f95bc98a424b386848ed1481631404a82fd93</citedby><cites>FETCH-LOGICAL-c489t-b2ec38b0543de5306738b103f647f95bc98a424b386848ed1481631404a82fd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0031942200004891$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=933071$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11314950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00291415$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Chassaigne, Hubert</creatorcontrib><creatorcontrib>Vacchina, Véronique</creatorcontrib><creatorcontrib>Kutchan, Toni M</creatorcontrib><creatorcontrib>Zenk, Meinhart H</creatorcontrib><title>Identification of phytochelatin-related peptides in maize seedlings exposed to cadmium and obtained enzymatically in vitro</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>An analytical strategy based on the sensitivity of electrospray tandem mass spectrometry following a simplified and reproducible sample preparation procedure was evaluated for the determination of Cd-induced phytochelatins (PC) and related peptides in four maize varieties. In addition to the three known families of PC (PC, desGly-PC and iso-PC(Glu)) that were observed, novel PC and desGly-PC homologues lacking the N-terminal γ-linked Glu were isolated from maize root extracts for the first time. Additionally the complete sequence of iso-PC
3(Glu) was determined by tandem mass spectrometry. Peptides obtained in vivo and in vitro as the result of the reaction of glutathione with the enzyme phytochelatin synthase were compared. Minor forms detected from in vitro reactions include compounds with intramolecular or intermolecular disulfide bonds resulting from the oxidation of SH groups, phytochelatin homologues lacking the N-terminal γ-linked Glu, and new PC-related peptides with a Cys–Cys motif. Since peptides lacking a γGlu residue could be generated as artifacts in electrospray mass spectrometry, the application of capillary electrophoresis with online electrospray mass spectrometry allowed the separation and detection of such peptides as endogenous molecules present in planta and as products of in vitro reactions.</description><subject>Aminoacyltransferases - metabolism</subject><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Cadmium</subject><subject>Cadmium - pharmacology</subject><subject>Cell biochemistry</subject><subject>Cell physiology</subject><subject>Chemical Sciences</subject><subject>Disulfides - analysis</subject><subject>Electrospray mass spectrometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione - metabolism</subject><subject>Maize seedlings</subject><subject>Metalloproteins - biosynthesis</subject><subject>Metalloproteins - chemistry</subject><subject>Metalloproteins - isolation & purification</subject><subject>Phytochelatin-related peptides</subject><subject>Phytochelatins</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - biosynthesis</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - isolation & purification</subject><subject>Proteins</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Zea mays</subject><subject>Zea mays - drug effects</subject><subject>Zea mays - metabolism</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo7uzqR1ACgriH1kon_e-0LIvrLgx4UM8hnVQ7ke6kTTKDM5_e9M4wHj0VKX55VfUeIW8YfGTA6k_fADgrOlGWHwCuAUTbFewZWbG24QVvAJ6T1Rm5IJcx_gKAqqrrl-SCMc5EV8GKHB4NumQHq1Wy3lE_0HmzT15vcMwdV4SloqEzzskajNQ6Oil7QBoRzWjdz0jxz-xjZpKnWpnJbieqnKG-T8q63Ed32E9ZTatx3C8CO5uCf0VeDGqM-PpUr8iP-8_f7x6K9dcvj3e360Lnm1LRl6h520MluMGKQ93kFwM-1KIZuqrXXatEKXre1q1o0TDRsjqfB0K15WA6fkWuj7obNco52EmFvfTKyofbtVx6AGXHBKt2LLPvj-wc_O8txiQnGzWOo3Lot1E2DdQMujKD1RHUwccYcDgrM5BLQPIpILm4nwfIp4DkMuDtacC2n9D8-3VKJAPvToCK2a8hKKdtPHMd59AsMjdHCrNxO4tBRm3RaTQ2oE7SePufRf4ChECsqQ</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Chassaigne, Hubert</creator><creator>Vacchina, Véronique</creator><creator>Kutchan, Toni M</creator><creator>Zenk, Meinhart H</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20010401</creationdate><title>Identification of phytochelatin-related peptides in maize seedlings exposed to cadmium and obtained enzymatically in vitro</title><author>Chassaigne, Hubert ; Vacchina, Véronique ; Kutchan, Toni M ; Zenk, Meinhart H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-b2ec38b0543de5306738b103f647f95bc98a424b386848ed1481631404a82fd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aminoacyltransferases - metabolism</topic><topic>Analytical chemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Binding and carrier proteins</topic><topic>Biological and medical sciences</topic><topic>Cadmium</topic><topic>Cadmium - pharmacology</topic><topic>Cell biochemistry</topic><topic>Cell physiology</topic><topic>Chemical Sciences</topic><topic>Disulfides - analysis</topic><topic>Electrospray mass spectrometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione - metabolism</topic><topic>Maize seedlings</topic><topic>Metalloproteins - biosynthesis</topic><topic>Metalloproteins - chemistry</topic><topic>Metalloproteins - isolation & purification</topic><topic>Phytochelatin-related peptides</topic><topic>Phytochelatins</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - biosynthesis</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - isolation & purification</topic><topic>Proteins</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Zea mays</topic><topic>Zea mays - drug effects</topic><topic>Zea mays - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chassaigne, Hubert</creatorcontrib><creatorcontrib>Vacchina, Véronique</creatorcontrib><creatorcontrib>Kutchan, Toni M</creatorcontrib><creatorcontrib>Zenk, Meinhart H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chassaigne, Hubert</au><au>Vacchina, Véronique</au><au>Kutchan, Toni M</au><au>Zenk, Meinhart H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of phytochelatin-related peptides in maize seedlings exposed to cadmium and obtained enzymatically in vitro</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>56</volume><issue>7</issue><spage>657</spage><epage>668</epage><pages>657-668</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>An analytical strategy based on the sensitivity of electrospray tandem mass spectrometry following a simplified and reproducible sample preparation procedure was evaluated for the determination of Cd-induced phytochelatins (PC) and related peptides in four maize varieties. In addition to the three known families of PC (PC, desGly-PC and iso-PC(Glu)) that were observed, novel PC and desGly-PC homologues lacking the N-terminal γ-linked Glu were isolated from maize root extracts for the first time. Additionally the complete sequence of iso-PC
3(Glu) was determined by tandem mass spectrometry. Peptides obtained in vivo and in vitro as the result of the reaction of glutathione with the enzyme phytochelatin synthase were compared. Minor forms detected from in vitro reactions include compounds with intramolecular or intermolecular disulfide bonds resulting from the oxidation of SH groups, phytochelatin homologues lacking the N-terminal γ-linked Glu, and new PC-related peptides with a Cys–Cys motif. Since peptides lacking a γGlu residue could be generated as artifacts in electrospray mass spectrometry, the application of capillary electrophoresis with online electrospray mass spectrometry allowed the separation and detection of such peptides as endogenous molecules present in planta and as products of in vitro reactions.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>11314950</pmid><doi>10.1016/S0031-9422(00)00489-1</doi><tpages>12</tpages></addata></record> |
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| ispartof | Phytochemistry (Oxford), 2001-04, Vol.56 (7), p.657-668 |
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| subjects | Aminoacyltransferases - metabolism Analytical chemistry Analytical, structural and metabolic biochemistry Binding and carrier proteins Biological and medical sciences Cadmium Cadmium - pharmacology Cell biochemistry Cell physiology Chemical Sciences Disulfides - analysis Electrospray mass spectrometry Fundamental and applied biological sciences. Psychology Glutathione - metabolism Maize seedlings Metalloproteins - biosynthesis Metalloproteins - chemistry Metalloproteins - isolation & purification Phytochelatin-related peptides Phytochelatins Plant physiology and development Plant Proteins - biosynthesis Plant Proteins - chemistry Plant Proteins - isolation & purification Proteins Spectrometry, Mass, Electrospray Ionization Zea mays Zea mays - drug effects Zea mays - metabolism |
| title | Identification of phytochelatin-related peptides in maize seedlings exposed to cadmium and obtained enzymatically in vitro |
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