Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA
A new multidetection biosensor has been developed using the electrochemical properties of cylinder-shaped conducting polypyrrole grown on miniaturized graphite electrodes. Our objective was to conceive a sensitive, labelless and real-time DNA sensor for biomedical diagnosis. In a first step, copolym...
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| Veröffentlicht in: | Biosensors & bioelectronics 2007-12, Vol.23 (5), p.735-740 |
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| container_title | Biosensors & bioelectronics |
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| creator | Bouchet, Aurélie Chaix, Carole Marquette, Christophe A. Blum, Loïc J. Mandrand, Bernard |
| description | A new multidetection biosensor has been developed using the electrochemical properties of cylinder-shaped conducting polypyrrole grown on miniaturized graphite electrodes. Our objective was to conceive a sensitive, labelless and real-time DNA sensor for biomedical diagnosis. In a first step, copolymers bearing both ferrocene redox markers and oligonucleotide probes were selectively electro-addressed on microchip electrodes. Then, the study of their voltammetric response upon the addition of DNA targets revealed that the hybridization was efficiently transduced through the variation of ferrocene oxidation intensity. Using this technique, a good selectivity between Human Immunodeficiency Virus and Hepatitis B Virus targets was obtained. It was indeed possible to directly follow the hybridization. Complementary DNA detection limit reached 100
pM (3
fmol in 30
μL), which represents a good performance for such a practical, labelless and real-time sensor. |
| doi_str_mv | 10.1016/j.bios.2007.06.018 |
| format | Article |
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pM (3
fmol in 30
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pM (3
fmol in 30
μL), which represents a good performance for such a practical, labelless and real-time sensor.</description><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cyclic voltammetry</subject><subject>DNA, Viral - analysis</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Ferrocene</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - genetics</subject><subject>HIV - genetics</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Labelless DNA detection</subject><subject>Methods. Procedures. Technologies</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Probes</subject><subject>Polymers</subject><subject>Polypyrrole</subject><subject>Pyrroles</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2r1DAUhoMo3vHqH3Ah3Si4aM1Hmw9wM4wfVxh0oxs3IU1OnQxpMybthfn3pszg3ekqcHjOy8n7IPSS4IZgwt8dm97H3FCMRYN5g4l8hDZECla3lHWP0Qarjtcd5-wGPcv5iAtIFH6KboiQXGFFNujn7hz85CDV-WBO4CobJ7fY2U-_qlMM59M5pRigGmKqgukhBMi5ggB2TtEeYPTWhGpcwuwdzGXq41TFofrwdfscPRlMyPDi-t6iH58-ft_d1ftvn7_stvvatq2aa9Gx1ipFuKFM9YS0SjCsQECvmOwEdnKQhrSOs54JN0hB-qEHCkBcSx0l7Ba9veQeTNCn5EeTzjoar--2e73OMKZSUsHvV_bNhT2l-HuBPOvRZ1t-ZSaIS9ZcdrR0J_4LEsUlEZ0sIL2ANsWcEwx_TyBYr5r0Ua-a9KpJY66LprL06pq-9CO4h5WrlwK8vgIml4KHZCbr8wOnlOwYXoPeXzgoBd97SDpbD5MF51ORoV30_7rjDxwisFM</recordid><startdate>20071215</startdate><enddate>20071215</enddate><creator>Bouchet, Aurélie</creator><creator>Chaix, Carole</creator><creator>Marquette, Christophe A.</creator><creator>Blum, Loïc J.</creator><creator>Mandrand, Bernard</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-3019-0696</orcidid></search><sort><creationdate>20071215</creationdate><title>Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA</title><author>Bouchet, Aurélie ; Chaix, Carole ; Marquette, Christophe A. ; Blum, Loïc J. ; Mandrand, Bernard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-7534c9916a239b11497309e7eb938570d8f8a14d63b37df871bfbe2ee1d42d213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Cyclic voltammetry</topic><topic>DNA, Viral - analysis</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Ferrocene</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - genetics</topic><topic>HIV - genetics</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Labelless DNA detection</topic><topic>Methods. Procedures. 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Our objective was to conceive a sensitive, labelless and real-time DNA sensor for biomedical diagnosis. In a first step, copolymers bearing both ferrocene redox markers and oligonucleotide probes were selectively electro-addressed on microchip electrodes. Then, the study of their voltammetric response upon the addition of DNA targets revealed that the hybridization was efficiently transduced through the variation of ferrocene oxidation intensity. Using this technique, a good selectivity between Human Immunodeficiency Virus and Hepatitis B Virus targets was obtained. It was indeed possible to directly follow the hybridization. Complementary DNA detection limit reached 100
pM (3
fmol in 30
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| subjects | Biological and medical sciences Biosensors Biotechnology Cyclic voltammetry DNA, Viral - analysis Electrochemistry Electrodes Ferrocene Fundamental and applied biological sciences. Psychology Hepatitis B virus Hepatitis B virus - genetics HIV - genetics Human immunodeficiency virus Humans Labelless DNA detection Methods. Procedures. Technologies Nucleic Acid Hybridization Oligonucleotide Probes Polymers Polypyrrole Pyrroles Various methods and equipments |
| title | Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA |
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