Establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines
Mast cells (MCs) are tissue resident, hematopoietic stem cells-derived elements, distributed throughout the body. They are the pivotal mediating cells of allergic reactions. In addition, in mice, MCs play a critical role in the defense against several pathogens, such as bacteria, parasites and virus...
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| Veröffentlicht in: | Life sciences (1973) 2005-07, Vol.77 (7), p.808-823 |
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| creator | Femenia, Françoise Arock, Michel Leriche, Laurence Delouis, Claude Millet, Gaëlle Ben Hamouda, Nadine Cote, Martine Alliot, Annie Lilin, Thomas Pinton, Alain Iannucceli, Nathalie Parodi, André Laurent Boireau, Pascal |
| description | Mast cells (MCs) are tissue resident, hematopoietic stem cells-derived elements, distributed throughout the body. They are the pivotal mediating cells of allergic reactions. In addition, in mice, MCs play a critical role in the defense against several pathogens, such as bacteria, parasites and viruses. Whereas the biology of rodent and human MCs has been extensively studied using in vitro derived populations, the role of MCs in pigs has not yet been evaluated, given the very low availability of pure porcine MCs populations. In the present report, we describe an original method to obtain continuous factor-dependent normal pig MCs (PMC) lines from fetal hematopoietic progenitors. These Stem Cell Factor (SCF) and Interleukin-3- (IL-3)-dependent PMC lines retain their capacity to growth after conventional freezing methods and exhibit most of the morphological and biochemical properties of normal, although immature, MCs, including metachromatic granules containing sulfated polysaccharides, the expression of c-kit and high-affinity IgE receptors (FcɛRI), and the ability to store histamine that is released upon cross-linking of FcɛRI. In vitro derived PMC lines might thus be valuable tools to further investigate the reactivity of these elements towards several parasites frequently encountered in pig, such as, but not limited to,
Ascaris suum, Trichinella spiralis or
Trichuris suis, or towards antigens derived from these pathogens. |
| doi_str_mv | 10.1016/j.lfs.2005.01.017 |
| format | Article |
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Ascaris suum, Trichinella spiralis or
Trichuris suis, or towards antigens derived from these pathogens.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2005.01.017</identifier><identifier>PMID: 15896811</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Ascaris suum ; Cell Culture Techniques ; Cell Culture Techniques - methods ; Cell Line ; Cell Lineage ; Cell Lineage - physiology ; Continuous cell lines ; Genotype ; Hematopoietic Stem Cells ; Hematopoietic Stem Cells - cytology ; Histamine ; Histamine - metabolism ; Immunology ; Karyotyping ; Life Sciences ; Mast cell ; Mast Cells ; Mast Cells - metabolism ; Mast Cells - ultrastructure ; Microsatellite Repeats ; Microsatellite Repeats - genetics ; Microscopy, Electron, Transmission ; Parasites ; Pig ; Proto-Oncogene Proteins c-kit ; Proto-Oncogene Proteins c-kit - metabolism ; Receptors, IgE ; Receptors, IgE - metabolism ; Sus scrofa ; Trichinella spiralis</subject><ispartof>Life sciences (1973), 2005-07, Vol.77 (7), p.808-823</ispartof><rights>2005 Elsevier Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-f7e9c6433c76319bfed61a337c99d28c999c627a156633c8dc836ee3a7d283c03</citedby><cites>FETCH-LOGICAL-c482t-f7e9c6433c76319bfed61a337c99d28c999c627a156633c8dc836ee3a7d283c03</cites><orcidid>0000-0003-3035-5454 ; 0000-0002-3998-7792 ; 0000-0002-0809-7180 ; 0000-0002-1232-9749</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320505002535$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15896811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00282512$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Femenia, Françoise</creatorcontrib><creatorcontrib>Arock, Michel</creatorcontrib><creatorcontrib>Leriche, Laurence</creatorcontrib><creatorcontrib>Delouis, Claude</creatorcontrib><creatorcontrib>Millet, Gaëlle</creatorcontrib><creatorcontrib>Ben Hamouda, Nadine</creatorcontrib><creatorcontrib>Cote, Martine</creatorcontrib><creatorcontrib>Alliot, Annie</creatorcontrib><creatorcontrib>Lilin, Thomas</creatorcontrib><creatorcontrib>Pinton, Alain</creatorcontrib><creatorcontrib>Iannucceli, Nathalie</creatorcontrib><creatorcontrib>Parodi, André Laurent</creatorcontrib><creatorcontrib>Boireau, Pascal</creatorcontrib><title>Establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Mast cells (MCs) are tissue resident, hematopoietic stem cells-derived elements, distributed throughout the body. They are the pivotal mediating cells of allergic reactions. In addition, in mice, MCs play a critical role in the defense against several pathogens, such as bacteria, parasites and viruses. Whereas the biology of rodent and human MCs has been extensively studied using in vitro derived populations, the role of MCs in pigs has not yet been evaluated, given the very low availability of pure porcine MCs populations. In the present report, we describe an original method to obtain continuous factor-dependent normal pig MCs (PMC) lines from fetal hematopoietic progenitors. These Stem Cell Factor (SCF) and Interleukin-3- (IL-3)-dependent PMC lines retain their capacity to growth after conventional freezing methods and exhibit most of the morphological and biochemical properties of normal, although immature, MCs, including metachromatic granules containing sulfated polysaccharides, the expression of c-kit and high-affinity IgE receptors (FcɛRI), and the ability to store histamine that is released upon cross-linking of FcɛRI. In vitro derived PMC lines might thus be valuable tools to further investigate the reactivity of these elements towards several parasites frequently encountered in pig, such as, but not limited to,
Ascaris suum, Trichinella spiralis or
Trichuris suis, or towards antigens derived from these pathogens.</description><subject>Animals</subject><subject>Ascaris suum</subject><subject>Cell Culture Techniques</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line</subject><subject>Cell Lineage</subject><subject>Cell Lineage - physiology</subject><subject>Continuous cell lines</subject><subject>Genotype</subject><subject>Hematopoietic Stem Cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Histamine</subject><subject>Histamine - metabolism</subject><subject>Immunology</subject><subject>Karyotyping</subject><subject>Life Sciences</subject><subject>Mast cell</subject><subject>Mast Cells</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - ultrastructure</subject><subject>Microsatellite Repeats</subject><subject>Microsatellite Repeats - genetics</subject><subject>Microscopy, Electron, Transmission</subject><subject>Parasites</subject><subject>Pig</subject><subject>Proto-Oncogene Proteins c-kit</subject><subject>Proto-Oncogene Proteins c-kit - metabolism</subject><subject>Receptors, IgE</subject><subject>Receptors, IgE - metabolism</subject><subject>Sus scrofa</subject><subject>Trichinella spiralis</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-LUzEQx4Mobl39A7xIToKHV_OjL8nD07Ksu0LBi55DmszbpryX1CQt7P71TmnRm8KQwMxnvszMl5D3nC054-rzbjmNdSkY65eMY-gXZMGNHjqmJH9JFoyJVScF66_Im1p3DMFey9fkivdmUIbzBZnvanObKdbtDKlRlwL1W1ecb1Dis2sxJ5pH6nNqMR3yodItzK7lfY7Qoqf7kh8hxZZL7QK2HCHQfXykKZfZTXR2tVEP00SnmKC-Ja9GN1V4d_mvyc-vdz9uH7r19_tvtzfrzq-MaN2oYfBqJaXXuMiwGSEo7qTUfhiCMPhiWWjHe6UQMsEbqQCk01iVnslr8umsu3WT3Zc4u_Jks4v24WZtTzm8jBE9F0eO7Mczi6v8OkBtdo71NLJLgPtapc1ghFH_BTlivRYCQX4Gfcm1Fhj_jMCZPRlndxaNsyfjLOMYGns-XMQPmxnC346LUwh8OQOAdztGKLb6CMlDiAV8syHHf8j_Bt2IqhQ</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Femenia, Françoise</creator><creator>Arock, Michel</creator><creator>Leriche, Laurence</creator><creator>Delouis, Claude</creator><creator>Millet, Gaëlle</creator><creator>Ben Hamouda, Nadine</creator><creator>Cote, Martine</creator><creator>Alliot, Annie</creator><creator>Lilin, Thomas</creator><creator>Pinton, Alain</creator><creator>Iannucceli, Nathalie</creator><creator>Parodi, André Laurent</creator><creator>Boireau, Pascal</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-3035-5454</orcidid><orcidid>https://orcid.org/0000-0002-3998-7792</orcidid><orcidid>https://orcid.org/0000-0002-0809-7180</orcidid><orcidid>https://orcid.org/0000-0002-1232-9749</orcidid></search><sort><creationdate>20050701</creationdate><title>Establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines</title><author>Femenia, Françoise ; 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They are the pivotal mediating cells of allergic reactions. In addition, in mice, MCs play a critical role in the defense against several pathogens, such as bacteria, parasites and viruses. Whereas the biology of rodent and human MCs has been extensively studied using in vitro derived populations, the role of MCs in pigs has not yet been evaluated, given the very low availability of pure porcine MCs populations. In the present report, we describe an original method to obtain continuous factor-dependent normal pig MCs (PMC) lines from fetal hematopoietic progenitors. These Stem Cell Factor (SCF) and Interleukin-3- (IL-3)-dependent PMC lines retain their capacity to growth after conventional freezing methods and exhibit most of the morphological and biochemical properties of normal, although immature, MCs, including metachromatic granules containing sulfated polysaccharides, the expression of c-kit and high-affinity IgE receptors (FcɛRI), and the ability to store histamine that is released upon cross-linking of FcɛRI. In vitro derived PMC lines might thus be valuable tools to further investigate the reactivity of these elements towards several parasites frequently encountered in pig, such as, but not limited to,
Ascaris suum, Trichinella spiralis or
Trichuris suis, or towards antigens derived from these pathogens.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>15896811</pmid><doi>10.1016/j.lfs.2005.01.017</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0003-3035-5454</orcidid><orcidid>https://orcid.org/0000-0002-3998-7792</orcidid><orcidid>https://orcid.org/0000-0002-0809-7180</orcidid><orcidid>https://orcid.org/0000-0002-1232-9749</orcidid></addata></record> |
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| ispartof | Life sciences (1973), 2005-07, Vol.77 (7), p.808-823 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Ascaris suum Cell Culture Techniques Cell Culture Techniques - methods Cell Line Cell Lineage Cell Lineage - physiology Continuous cell lines Genotype Hematopoietic Stem Cells Hematopoietic Stem Cells - cytology Histamine Histamine - metabolism Immunology Karyotyping Life Sciences Mast cell Mast Cells Mast Cells - metabolism Mast Cells - ultrastructure Microsatellite Repeats Microsatellite Repeats - genetics Microscopy, Electron, Transmission Parasites Pig Proto-Oncogene Proteins c-kit Proto-Oncogene Proteins c-kit - metabolism Receptors, IgE Receptors, IgE - metabolism Sus scrofa Trichinella spiralis |
| title | Establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines |
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