Proteome analysis of differentiating human myoblasts by dialysis-assisted two-dimensional gel electrophoresis (DAGE)

In the present study, modifications in cytosolic expressed proteins during human myoblast differentiation were studied by dialysis-assisted 2-DE (DAGE, [1]). About 1000 spots were analysed on the 5th and 13th day of differentiation with a dynamic range of protein expression exceeding 1000-fold. Duri...

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Veröffentlicht in:	Proteomics (Weinheim) 2008-01, Vol.8 (2), p.264-278
Hauptverfasser:	Gonnet, Florence, Bouazza, Belaid, Millot, Gaël Armel, Ziaei, Simin, Garcia, Luis, Butler-Browne, Gillian S, Mouly, Vincent, Tortajada, Jeanine, Danos, Olivier, Svinartchouk, Fédor
Format:	Artikel
Sprache:	eng
Schlagworte:	2-D PAGE Analytical, structural and metabolic biochemistry Biochemistry, Molecular Biology Biological and medical sciences Blotting, Western Cell Differentiation Cytosol - chemistry Dialysis - methods Differential expression Electrophoresis, Gel, Two-Dimensional - methods Factor XIII - analysis Fundamental and applied biological sciences. Psychology Genomics Guanine Nucleotide Dissociation Inhibitors - analysis Heterogeneous-Nuclear Ribonucleoprotein K - analysis Human Humans Life Sciences Mass spectrometry Miscellaneous Myoblasts - chemistry Protein identification Proteins Proteomics - methods rho-Specific Guanine Nucleotide Dissociation Inhibitors Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization STAT1 Transcription Factor - analysis Stathmin - analysis
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