Comparative interaction kinetics of two recombinant fabs and of the corresponding antibodies directed to the coat protein of tobacco mosaic virus

Two recombinant Fab fragments, 57P and 174P, recognizing peptide 134–146 of the coat protein of tobacco mosaic virus have been cloned, sequenced and expressed in Escherichia coli. They differ by 15 amino acid changes in the sequence of their variable region. The interaction kinetics of the Fabs with...

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Veröffentlicht in:	Journal of molecular recognition 1996-01, Vol.9 (1), p.39-51
Hauptverfasser:	Chatellier, Jean, Rauffer-Bruyère, Nathalie, Van Regenmortel, Marc H. V., Altschuh, Danièle, Weiss, Etienne
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibodies, Monoclonal Antibodies, Monoclonal - immunology Antibodies, Monoclonal - metabolism Antibodies, Viral Antibodies, Viral - chemistry Antibodies, Viral - immunology Antibodies, Viral - metabolism Antigen Presentation BIAcoreTM Biochemistry, Molecular Biology Biosensing Techniques Capsid Capsid - chemistry Capsid - immunology Capsid - metabolism cloning strategy Cloning, Molecular DNA Primers Escherichia coli Escherichia coli - genetics filter screening Immunoglobulin Fab Fragments Immunoglobulin Fab Fragments - chemistry Immunoglobulin Fab Fragments - immunology Immunoglobulin Fab Fragments - metabolism Immunoglobulin Variable Region Immunoglobulin Variable Region - chemistry kinetic rate constants Kinetics Life Sciences Models, Molecular Molecular Sequence Data Mutation Peptide Fragments Peptide Fragments - chemistry Peptide Fragments - metabolism Peptide Fragments - pharmacology peptide-antibody interaction recombinant Fab Recombinant Proteins Recombinant Proteins - immunology Recombinant Proteins - metabolism Tobacco Mosaic Virus Tobacco Mosaic Virus - immunology tobacco mosaic virus protein
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