Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction
Detection of toxic substances interfering with endocrine system is one of the major preoccupations of the European community. A whole-cell bioassay for pollution detection based on stress induction has been designed. Well characterized toxicants, cadmium chloride and thiram (a dithiocarbamate fungic...
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| Veröffentlicht in: | Journal of biotechnology 2006-07, Vol.124 (2), p.392-402 |
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| creator | Mandon, C.A. Diaz-Latoud, C. Arrigo, A.-P. Blum, L.J. |
| description | Detection of toxic substances interfering with endocrine system is one of the major preoccupations of the European community. A whole-cell bioassay for pollution detection based on stress induction has been designed. Well characterized toxicants, cadmium chloride and thiram (a dithiocarbamate fungicide), were used to optimize the detection conditions such as time-course conditions, cell line and reporter gene to be used. HeLa cells containing the firefly luciferase (
luc) reporter gene under the control of the
Drosophila melanogaster hsp22 promoter were compared to liver cells (HepG2) containing the same stress gene promoter fused either to the
luc or the EGFP (Enhanced-Green Fluorescent Protein) gene. The sensitivity of the obtained bioassay was found to be enhanced by the concomitant use of liver cells and EGFP reporter gene. The detection limits of the toxicants were then lowered from 1 to 0.1
μM and from 1 to 0.01
μM for CdCl
2 and thiram, respectively. |
| doi_str_mv | 10.1016/j.jbiotec.2006.01.004 |
| format | Article |
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luc) reporter gene under the control of the
Drosophila melanogaster hsp22 promoter were compared to liver cells (HepG2) containing the same stress gene promoter fused either to the
luc or the EGFP (Enhanced-Green Fluorescent Protein) gene. The sensitivity of the obtained bioassay was found to be enhanced by the concomitant use of liver cells and EGFP reporter gene. The detection limits of the toxicants were then lowered from 1 to 0.1
μM and from 1 to 0.01
μM for CdCl
2 and thiram, respectively.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2006.01.004</identifier><identifier>PMID: 16492392</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>bioassays ; Biological and medical sciences ; Biological Assay ; Biological Assay - methods ; bioluminescence ; Biotechnology ; Cadmium ; Cadmium Chloride ; Cadmium Chloride - analysis ; Cadmium Chloride - toxicity ; cell lines ; Cellular Biology ; Drosophila melanogaster ; Drosophila Proteins ; Drosophila Proteins - genetics ; EGFP ; enhanced green fluorescent protein ; Environmental Pollutants ; Environmental Pollutants - analysis ; Environmental Pollutants - toxicity ; Firefly luciferase ; fluorescence ; Fundamental and applied biological sciences. Psychology ; fungicide residues ; Fungicides, Industrial ; Fungicides, Industrial - analysis ; Fungicides, Industrial - toxicity ; gene expression ; Genes, Reporter ; Genes, Reporter - genetics ; green fluorescent protein ; Green Fluorescent Proteins ; Green Fluorescent Proteins - genetics ; Heat-Shock Proteins ; Heat-Shock Proteins - genetics ; HeLa cells ; HeLa Cells - drug effects ; HeLa Cells - enzymology ; Hepatocytes ; Hepatocytes - drug effects ; Hepatocytes - enzymology ; hsp22 promoter ; Humans ; Life Sciences ; liver neoplasms ; luciferase ; Luciferases, Firefly ; Luciferases, Firefly - genetics ; Luciferases, Firefly - metabolism ; promoter regions ; recombinant DNA ; reporter genes ; Thiram ; Thiram - analysis ; Thiram - toxicity ; toxicity testing ; uterine cervical neoplasms ; Whole-cell bioassay</subject><ispartof>Journal of biotechnology, 2006-07, Vol.124 (2), p.392-402</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-ccc8577edb6be3f77ee6e966b85d0bf34f004c2380f0172bb3b660ca35856f043</citedby><cites>FETCH-LOGICAL-c482t-ccc8577edb6be3f77ee6e966b85d0bf34f004c2380f0172bb3b660ca35856f043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiotec.2006.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17900661$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16492392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00193407$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Mandon, C.A.</creatorcontrib><creatorcontrib>Diaz-Latoud, C.</creatorcontrib><creatorcontrib>Arrigo, A.-P.</creatorcontrib><creatorcontrib>Blum, L.J.</creatorcontrib><title>Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>Detection of toxic substances interfering with endocrine system is one of the major preoccupations of the European community. A whole-cell bioassay for pollution detection based on stress induction has been designed. Well characterized toxicants, cadmium chloride and thiram (a dithiocarbamate fungicide), were used to optimize the detection conditions such as time-course conditions, cell line and reporter gene to be used. HeLa cells containing the firefly luciferase (
luc) reporter gene under the control of the
Drosophila melanogaster hsp22 promoter were compared to liver cells (HepG2) containing the same stress gene promoter fused either to the
luc or the EGFP (Enhanced-Green Fluorescent Protein) gene. The sensitivity of the obtained bioassay was found to be enhanced by the concomitant use of liver cells and EGFP reporter gene. The detection limits of the toxicants were then lowered from 1 to 0.1
μM and from 1 to 0.01
μM for CdCl
2 and thiram, respectively.</description><subject>bioassays</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Biological Assay - methods</subject><subject>bioluminescence</subject><subject>Biotechnology</subject><subject>Cadmium</subject><subject>Cadmium Chloride</subject><subject>Cadmium Chloride - analysis</subject><subject>Cadmium Chloride - toxicity</subject><subject>cell lines</subject><subject>Cellular Biology</subject><subject>Drosophila melanogaster</subject><subject>Drosophila Proteins</subject><subject>Drosophila Proteins - genetics</subject><subject>EGFP</subject><subject>enhanced green fluorescent protein</subject><subject>Environmental Pollutants</subject><subject>Environmental Pollutants - analysis</subject><subject>Environmental Pollutants - toxicity</subject><subject>Firefly luciferase</subject><subject>fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungicide residues</subject><subject>Fungicides, Industrial</subject><subject>Fungicides, Industrial - analysis</subject><subject>Fungicides, Industrial - toxicity</subject><subject>gene expression</subject><subject>Genes, Reporter</subject><subject>Genes, Reporter - genetics</subject><subject>green fluorescent protein</subject><subject>Green Fluorescent Proteins</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Heat-Shock Proteins</subject><subject>Heat-Shock Proteins - genetics</subject><subject>HeLa cells</subject><subject>HeLa Cells - drug effects</subject><subject>HeLa Cells - enzymology</subject><subject>Hepatocytes</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - enzymology</subject><subject>hsp22 promoter</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>liver neoplasms</subject><subject>luciferase</subject><subject>Luciferases, Firefly</subject><subject>Luciferases, Firefly - genetics</subject><subject>Luciferases, Firefly - metabolism</subject><subject>promoter regions</subject><subject>recombinant DNA</subject><subject>reporter genes</subject><subject>Thiram</subject><subject>Thiram - analysis</subject><subject>Thiram - toxicity</subject><subject>toxicity testing</subject><subject>uterine cervical neoplasms</subject><subject>Whole-cell bioassay</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEotvCIwC-UIlDwthOnKQXVC1_irQSB-jZsp1x16skXuykqC_C8-KwET1ysj36zXzf-MuyVxQKClS8PxQH7fyEpmAAogBaAJRPsg1tap6XjeBPs03impyKSpxl5zEeIBFtRZ9nZ1SULeMt22S_P7pp77xRQatBTUjsPN454zokqR7UQDpMIpPz4xXZ-uGogot-JN6SJN_PgxsxGhwnosaO2H72YX3_2vsec4N9v5AqRvUQiVYRO5L69_HIGIlToiM5Bj-kVQJxYzf_1XqRPbOqj_hyPS-y28-ffmxv8t23L1-317vclA2bcmNMU9U1dlpo5DbdUGArhG6qDrTlpU0rG8YbsEBrpjXXQoBRvGoqYaHkF9m709y96uUxuEGFB-mVkzfXO7nUAGjLS6jvaWIvT2yy-3PGOMnBxWU_NaKfo0wCTEDdJrA6gSb4GAPaf5MpyCU8eZBreHIJTwJNOouZ16vArAfsHrvWtBLwdgVUNKq3QY3GxUeubtM0sTh9c-Ks8lLdpcTk7XcGlAOFpmGUJ-LDicD0ufcOg4zG4WiwcyGlLTvv_mP2D-uLxr0</recordid><startdate>20060713</startdate><enddate>20060713</enddate><creator>Mandon, C.A.</creator><creator>Diaz-Latoud, C.</creator><creator>Arrigo, A.-P.</creator><creator>Blum, L.J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>1XC</scope></search><sort><creationdate>20060713</creationdate><title>Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction</title><author>Mandon, C.A. ; Diaz-Latoud, C. ; Arrigo, A.-P. ; Blum, L.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-ccc8577edb6be3f77ee6e966b85d0bf34f004c2380f0172bb3b660ca35856f043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>bioassays</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Biological Assay - methods</topic><topic>bioluminescence</topic><topic>Biotechnology</topic><topic>Cadmium</topic><topic>Cadmium Chloride</topic><topic>Cadmium Chloride - analysis</topic><topic>Cadmium Chloride - toxicity</topic><topic>cell lines</topic><topic>Cellular Biology</topic><topic>Drosophila melanogaster</topic><topic>Drosophila Proteins</topic><topic>Drosophila Proteins - genetics</topic><topic>EGFP</topic><topic>enhanced green fluorescent protein</topic><topic>Environmental Pollutants</topic><topic>Environmental Pollutants - analysis</topic><topic>Environmental Pollutants - toxicity</topic><topic>Firefly luciferase</topic><topic>fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungicide residues</topic><topic>Fungicides, Industrial</topic><topic>Fungicides, Industrial - analysis</topic><topic>Fungicides, Industrial - toxicity</topic><topic>gene expression</topic><topic>Genes, Reporter</topic><topic>Genes, Reporter - genetics</topic><topic>green fluorescent protein</topic><topic>Green Fluorescent Proteins</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Heat-Shock Proteins</topic><topic>Heat-Shock Proteins - genetics</topic><topic>HeLa cells</topic><topic>HeLa Cells - drug effects</topic><topic>HeLa Cells - enzymology</topic><topic>Hepatocytes</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - enzymology</topic><topic>hsp22 promoter</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>liver neoplasms</topic><topic>luciferase</topic><topic>Luciferases, Firefly</topic><topic>Luciferases, Firefly - genetics</topic><topic>Luciferases, Firefly - metabolism</topic><topic>promoter regions</topic><topic>recombinant DNA</topic><topic>reporter genes</topic><topic>Thiram</topic><topic>Thiram - analysis</topic><topic>Thiram - toxicity</topic><topic>toxicity testing</topic><topic>uterine cervical neoplasms</topic><topic>Whole-cell bioassay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mandon, C.A.</creatorcontrib><creatorcontrib>Diaz-Latoud, C.</creatorcontrib><creatorcontrib>Arrigo, A.-P.</creatorcontrib><creatorcontrib>Blum, L.J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mandon, C.A.</au><au>Diaz-Latoud, C.</au><au>Arrigo, A.-P.</au><au>Blum, L.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2006-07-13</date><risdate>2006</risdate><volume>124</volume><issue>2</issue><spage>392</spage><epage>402</epage><pages>392-402</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>Detection of toxic substances interfering with endocrine system is one of the major preoccupations of the European community. A whole-cell bioassay for pollution detection based on stress induction has been designed. Well characterized toxicants, cadmium chloride and thiram (a dithiocarbamate fungicide), were used to optimize the detection conditions such as time-course conditions, cell line and reporter gene to be used. HeLa cells containing the firefly luciferase (
luc) reporter gene under the control of the
Drosophila melanogaster hsp22 promoter were compared to liver cells (HepG2) containing the same stress gene promoter fused either to the
luc or the EGFP (Enhanced-Green Fluorescent Protein) gene. The sensitivity of the obtained bioassay was found to be enhanced by the concomitant use of liver cells and EGFP reporter gene. The detection limits of the toxicants were then lowered from 1 to 0.1
μM and from 1 to 0.01
μM for CdCl
2 and thiram, respectively.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>16492392</pmid><doi>10.1016/j.jbiotec.2006.01.004</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of biotechnology, 2006-07, Vol.124 (2), p.392-402 |
| issn | 0168-1656 1873-4863 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | bioassays Biological and medical sciences Biological Assay Biological Assay - methods bioluminescence Biotechnology Cadmium Cadmium Chloride Cadmium Chloride - analysis Cadmium Chloride - toxicity cell lines Cellular Biology Drosophila melanogaster Drosophila Proteins Drosophila Proteins - genetics EGFP enhanced green fluorescent protein Environmental Pollutants Environmental Pollutants - analysis Environmental Pollutants - toxicity Firefly luciferase fluorescence Fundamental and applied biological sciences. Psychology fungicide residues Fungicides, Industrial Fungicides, Industrial - analysis Fungicides, Industrial - toxicity gene expression Genes, Reporter Genes, Reporter - genetics green fluorescent protein Green Fluorescent Proteins Green Fluorescent Proteins - genetics Heat-Shock Proteins Heat-Shock Proteins - genetics HeLa cells HeLa Cells - drug effects HeLa Cells - enzymology Hepatocytes Hepatocytes - drug effects Hepatocytes - enzymology hsp22 promoter Humans Life Sciences liver neoplasms luciferase Luciferases, Firefly Luciferases, Firefly - genetics Luciferases, Firefly - metabolism promoter regions recombinant DNA reporter genes Thiram Thiram - analysis Thiram - toxicity toxicity testing uterine cervical neoplasms Whole-cell bioassay |
| title | Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction |
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