Neuronal calcium sensor‐1 gene ncs‐1a is essential for semicircular canal formation in zebrafish inner ear
We have analyzed the functional role of neuronal calcium sensor‐1 (Ncs‐1) in zebrafish development. We identified two orthologs of the mammalian NCS‐1 gene. Full‐length cDNAs encoding zebrafish Ncs‐1a and Ncs‐1b polypeptides were cloned and characterized. Whole‐mount in situ hybridization revealed t...
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| Veröffentlicht in: | Journal of neurobiology 2005-09, Vol.64 (3), p.285-297 |
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| creator | Blasiole, Brian Kabbani, Nadine Boehmler, Wendy Thisse, Bernard Thisse, Christine Canfield, Victor Levenson, Robert |
| description | We have analyzed the functional role of neuronal calcium sensor‐1 (Ncs‐1) in zebrafish development. We identified two orthologs of the mammalian NCS‐1 gene. Full‐length cDNAs encoding zebrafish Ncs‐1a and Ncs‐1b polypeptides were cloned and characterized. Whole‐mount in situ hybridization revealed that ncs‐1a mRNA was expressed beginning at early somitogenesis. As development progressed, ncs‐1a mRNA was present throughout the embryo with expression detected in ventral hematopoietic mesoderm, pronephric tubules, CNS nuclei, and otic vesicle. By 4.5 days post fertilization (dpf), ncs‐1a expression was detected primarily in the brain. Expression of ncs‐1b mRNA was first detected at 36 hours post fertilization (hpf) and was restricted to the olfactory bulb. By 4.5 dpf, ncs‐1b was expressed at low levels throughout the brain. Knockdown of ncs‐1a mRNA translation with antisense morpholinos blocked formation of semicircular canals. These studies identify a novel function for ncs‐1a in inner ear development and suggest that this calcium sensor plays an important role in vestibular function. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005 |
| doi_str_mv | 10.1002/neu.20138 |
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We identified two orthologs of the mammalian NCS‐1 gene. Full‐length cDNAs encoding zebrafish Ncs‐1a and Ncs‐1b polypeptides were cloned and characterized. Whole‐mount in situ hybridization revealed that ncs‐1a mRNA was expressed beginning at early somitogenesis. As development progressed, ncs‐1a mRNA was present throughout the embryo with expression detected in ventral hematopoietic mesoderm, pronephric tubules, CNS nuclei, and otic vesicle. By 4.5 days post fertilization (dpf), ncs‐1a expression was detected primarily in the brain. Expression of ncs‐1b mRNA was first detected at 36 hours post fertilization (hpf) and was restricted to the olfactory bulb. By 4.5 dpf, ncs‐1b was expressed at low levels throughout the brain. Knockdown of ncs‐1a mRNA translation with antisense morpholinos blocked formation of semicircular canals. These studies identify a novel function for ncs‐1a in inner ear development and suggest that this calcium sensor plays an important role in vestibular function. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005</description><identifier>ISSN: 0022-3034</identifier><identifier>EISSN: 1097-4695</identifier><identifier>DOI: 10.1002/neu.20138</identifier><identifier>PMID: 15898063</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Amino Acid Sequence ; Animals ; Biochemistry, Molecular Biology ; Blotting, Western ; Brain ; Brain - embryology ; Calcium-Binding Proteins ; Calcium-Binding Proteins - genetics ; Cloning, Molecular ; ear development ; Freshwater ; Gene Expression Regulation, Developmental ; Humans ; In Situ Hybridization ; Life Sciences ; Molecular biology ; Molecular Sequence Data ; morpholinos ; neuronal calcium sensor ; Neuronal Calcium-Sensor Proteins ; Neuropeptides ; Neuropeptides - genetics ; Oligonucleotides, Antisense ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger ; RNA, Messenger - analysis ; Semicircular Canals ; Semicircular Canals - embryology ; Sequence Homology, Amino Acid ; Zebrafish ; Zebrafish Proteins ; Zebrafish Proteins - genetics</subject><ispartof>Journal of neurobiology, 2005-09, Vol.64 (3), p.285-297</ispartof><rights>Copyright © 2005 Wiley Periodicals, Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fneu.20138$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fneu.20138$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15898063$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00187454$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Blasiole, Brian</creatorcontrib><creatorcontrib>Kabbani, Nadine</creatorcontrib><creatorcontrib>Boehmler, Wendy</creatorcontrib><creatorcontrib>Thisse, Bernard</creatorcontrib><creatorcontrib>Thisse, Christine</creatorcontrib><creatorcontrib>Canfield, Victor</creatorcontrib><creatorcontrib>Levenson, Robert</creatorcontrib><title>Neuronal calcium sensor‐1 gene ncs‐1a is essential for semicircular canal formation in zebrafish inner ear</title><title>Journal of neurobiology</title><addtitle>J Neurobiol</addtitle><description>We have analyzed the functional role of neuronal calcium sensor‐1 (Ncs‐1) in zebrafish development. We identified two orthologs of the mammalian NCS‐1 gene. Full‐length cDNAs encoding zebrafish Ncs‐1a and Ncs‐1b polypeptides were cloned and characterized. Whole‐mount in situ hybridization revealed that ncs‐1a mRNA was expressed beginning at early somitogenesis. As development progressed, ncs‐1a mRNA was present throughout the embryo with expression detected in ventral hematopoietic mesoderm, pronephric tubules, CNS nuclei, and otic vesicle. By 4.5 days post fertilization (dpf), ncs‐1a expression was detected primarily in the brain. Expression of ncs‐1b mRNA was first detected at 36 hours post fertilization (hpf) and was restricted to the olfactory bulb. By 4.5 dpf, ncs‐1b was expressed at low levels throughout the brain. Knockdown of ncs‐1a mRNA translation with antisense morpholinos blocked formation of semicircular canals. These studies identify a novel function for ncs‐1a in inner ear development and suggest that this calcium sensor plays an important role in vestibular function. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biochemistry, Molecular Biology</subject><subject>Blotting, Western</subject><subject>Brain</subject><subject>Brain - embryology</subject><subject>Calcium-Binding Proteins</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Cloning, Molecular</subject><subject>ear development</subject><subject>Freshwater</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Molecular Sequence Data</subject><subject>morpholinos</subject><subject>neuronal calcium sensor</subject><subject>Neuronal Calcium-Sensor Proteins</subject><subject>Neuropeptides</subject><subject>Neuropeptides - genetics</subject><subject>Oligonucleotides, Antisense</subject><subject>Phylogeny</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger</subject><subject>RNA, Messenger - analysis</subject><subject>Semicircular Canals</subject><subject>Semicircular Canals - embryology</subject><subject>Sequence Homology, Amino Acid</subject><subject>Zebrafish</subject><subject>Zebrafish Proteins</subject><subject>Zebrafish Proteins - genetics</subject><issn>0022-3034</issn><issn>1097-4695</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9O3DAQxq2qqGxpD30B5FOlHgJjO47tI0L8k1ZwKWfLcSZdo8QBe0NFTzxCn5EnqZelcOQ0nu_7zciaj5BvDA4YAD-MOB9wYEJ_IAsGRlV1Y-RHsigerwSIepd8zvkGAIyR_BPZZVIbDY1YkHiJc5qiG6h3gw_zSDPGPKWnx7-M_sKINPq8aRwNmWIu7joUup9SIcfgQ_Lz4FIZj1t5dOswRRoi_YNtcn3Iq9JETBRd-kJ2ejdk_PpS98j16cnP4_NqeXV2cXy0rFaC1bpSDLhhvegMdFIp32CHsofWozQMFO-cQW4417I1ytdNK7zWzreqK5KWKPbIj-3elRvsbQqjSw92csGeHy3tRgNgWtWyvmeF_b5lb9N0N2Ne2zFkj8PgIk5zto0ud5Mg3gWZaoRgTBZw_wWc2xG71w_8P3sBDrfA7zDgw5sPdpOnLXna5zzt5cn180P8Aw-ak3o</recordid><startdate>20050905</startdate><enddate>20050905</enddate><creator>Blasiole, Brian</creator><creator>Kabbani, Nadine</creator><creator>Boehmler, Wendy</creator><creator>Thisse, Bernard</creator><creator>Thisse, Christine</creator><creator>Canfield, Victor</creator><creator>Levenson, Robert</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7TK</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20050905</creationdate><title>Neuronal calcium sensor‐1 gene ncs‐1a is essential for semicircular canal formation in zebrafish inner ear</title><author>Blasiole, Brian ; Kabbani, Nadine ; Boehmler, Wendy ; Thisse, Bernard ; Thisse, Christine ; Canfield, Victor ; Levenson, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h3148-710291f3d90d577c6ede5f0bce591072da9e292285b97c46b3c88acb7d28585e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biochemistry, Molecular Biology</topic><topic>Blotting, Western</topic><topic>Brain</topic><topic>Brain - embryology</topic><topic>Calcium-Binding Proteins</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Cloning, Molecular</topic><topic>ear development</topic><topic>Freshwater</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Life Sciences</topic><topic>Molecular biology</topic><topic>Molecular Sequence Data</topic><topic>morpholinos</topic><topic>neuronal calcium sensor</topic><topic>Neuronal Calcium-Sensor Proteins</topic><topic>Neuropeptides</topic><topic>Neuropeptides - genetics</topic><topic>Oligonucleotides, Antisense</topic><topic>Phylogeny</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger</topic><topic>RNA, Messenger - analysis</topic><topic>Semicircular Canals</topic><topic>Semicircular Canals - embryology</topic><topic>Sequence Homology, Amino Acid</topic><topic>Zebrafish</topic><topic>Zebrafish Proteins</topic><topic>Zebrafish Proteins - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Blasiole, Brian</creatorcontrib><creatorcontrib>Kabbani, Nadine</creatorcontrib><creatorcontrib>Boehmler, Wendy</creatorcontrib><creatorcontrib>Thisse, Bernard</creatorcontrib><creatorcontrib>Thisse, Christine</creatorcontrib><creatorcontrib>Canfield, Victor</creatorcontrib><creatorcontrib>Levenson, Robert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blasiole, Brian</au><au>Kabbani, Nadine</au><au>Boehmler, Wendy</au><au>Thisse, Bernard</au><au>Thisse, Christine</au><au>Canfield, Victor</au><au>Levenson, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuronal calcium sensor‐1 gene ncs‐1a is essential for semicircular canal formation in zebrafish inner ear</atitle><jtitle>Journal of neurobiology</jtitle><addtitle>J Neurobiol</addtitle><date>2005-09-05</date><risdate>2005</risdate><volume>64</volume><issue>3</issue><spage>285</spage><epage>297</epage><pages>285-297</pages><issn>0022-3034</issn><eissn>1097-4695</eissn><abstract>We have analyzed the functional role of neuronal calcium sensor‐1 (Ncs‐1) in zebrafish development. We identified two orthologs of the mammalian NCS‐1 gene. Full‐length cDNAs encoding zebrafish Ncs‐1a and Ncs‐1b polypeptides were cloned and characterized. Whole‐mount in situ hybridization revealed that ncs‐1a mRNA was expressed beginning at early somitogenesis. As development progressed, ncs‐1a mRNA was present throughout the embryo with expression detected in ventral hematopoietic mesoderm, pronephric tubules, CNS nuclei, and otic vesicle. By 4.5 days post fertilization (dpf), ncs‐1a expression was detected primarily in the brain. Expression of ncs‐1b mRNA was first detected at 36 hours post fertilization (hpf) and was restricted to the olfactory bulb. By 4.5 dpf, ncs‐1b was expressed at low levels throughout the brain. Knockdown of ncs‐1a mRNA translation with antisense morpholinos blocked formation of semicircular canals. 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| subjects | Amino Acid Sequence Animals Biochemistry, Molecular Biology Blotting, Western Brain Brain - embryology Calcium-Binding Proteins Calcium-Binding Proteins - genetics Cloning, Molecular ear development Freshwater Gene Expression Regulation, Developmental Humans In Situ Hybridization Life Sciences Molecular biology Molecular Sequence Data morpholinos neuronal calcium sensor Neuronal Calcium-Sensor Proteins Neuropeptides Neuropeptides - genetics Oligonucleotides, Antisense Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Messenger - analysis Semicircular Canals Semicircular Canals - embryology Sequence Homology, Amino Acid Zebrafish Zebrafish Proteins Zebrafish Proteins - genetics |
| title | Neuronal calcium sensor‐1 gene ncs‐1a is essential for semicircular canal formation in zebrafish inner ear |
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