Cloning and Characterization of the Human and Rat Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein (IGRP) Genes
Islet-specific glucose-6-phosphatase (G6Pase) catalytic subunit-related protein (IGRP) is a homolog of the catalytic subunit of G6Pase, the enzyme that catalyzes the terminal step of the gluconeogenic pathway. Its catalytic activity, however, has not been defined. Since IGRP gene expression is restr...
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creator | Martin, C C Bischof, L J Bergman, B Hornbuckle, L A Hilliker, C Frigeri, C Wahl, D Svitek, C A Wong, R Goldman, J K Oeser, J K Leprêtre, F Froguel, P O'Brien, R M Hutton, J C |
description | Islet-specific glucose-6-phosphatase (G6Pase) catalytic subunit-related protein (IGRP) is a homolog of the catalytic subunit
of G6Pase, the enzyme that catalyzes the terminal step of the gluconeogenic pathway. Its catalytic activity, however, has
not been defined. Since IGRP gene expression is restricted to islets, this suggests a possible role in the regulation of islet metabolism and, hence,
insulin secretion induced by metabolites. We report here a comparative analysis of the human, mouse, and rat IGRP genes. These studies aimed to identify conserved sequences that may be critical for IGRP function and that specify its restricted
tissue distribution. The single copy human IGRP gene has five exons of similar length and coding sequence to the mouse IGRP gene and is located on human chromosome 2q28â32 adjacent to the myosin heavy chain 1B gene. In contrast, the rat IGRP gene does not appear to encode a protein as a result of a series of deletions and insertions in the coding sequence. Moreover,
rat IGRP mRNA, unlike mouse and human IGRP mRNA, is not expressed in islets or islet-derived cell lines, an observation that
was traced by fusion gene analysis to a mutation of the TATA box motif in the mouse/human IGRP promoters to TGTA in the rat
sequence. The results provide a framework for the further analysis of the molecular basis for the tissue-restricted expression
of the IGRP gene and the identification of key amino acid sequences that determine its biological activity. |
doi_str_mv | 10.1074/jbc.M101549200 |
format | Article |
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of G6Pase, the enzyme that catalyzes the terminal step of the gluconeogenic pathway. Its catalytic activity, however, has
not been defined. Since IGRP gene expression is restricted to islets, this suggests a possible role in the regulation of islet metabolism and, hence,
insulin secretion induced by metabolites. We report here a comparative analysis of the human, mouse, and rat IGRP genes. These studies aimed to identify conserved sequences that may be critical for IGRP function and that specify its restricted
tissue distribution. The single copy human IGRP gene has five exons of similar length and coding sequence to the mouse IGRP gene and is located on human chromosome 2q28â32 adjacent to the myosin heavy chain 1B gene. In contrast, the rat IGRP gene does not appear to encode a protein as a result of a series of deletions and insertions in the coding sequence. Moreover,
rat IGRP mRNA, unlike mouse and human IGRP mRNA, is not expressed in islets or islet-derived cell lines, an observation that
was traced by fusion gene analysis to a mutation of the TATA box motif in the mouse/human IGRP promoters to TGTA in the rat
sequence. The results provide a framework for the further analysis of the molecular basis for the tissue-restricted expression
of the IGRP gene and the identification of key amino acid sequences that determine its biological activity.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M101549200</identifier><identifier>PMID: 11297555</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Catalytic Domain ; Cloning, Molecular ; Conserved Sequence ; Genetics ; Glucose-6-Phosphatase ; Glucose-6-Phosphatase - chemistry ; Glucose-6-Phosphatase - genetics ; Human genetics ; Humans ; IGRP gene ; Immunoenzyme Techniques ; Life Sciences ; Mice ; Molecular Sequence Data ; Promoter Regions (Genetics) ; Promoter Regions, Genetic ; Proteins ; Proteins - chemistry ; Proteins - genetics ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger ; RNA, Messenger - analysis ; Sequence Alignment</subject><ispartof>The Journal of biological chemistry, 2001-07, Vol.276 (27), p.25197-25207</ispartof><rights>Copyright</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3400-a66c9764b61d2deacc8f8e193d8fdf1628964db33805d9306386779cc668af9f3</citedby><cites>FETCH-LOGICAL-c3400-a66c9764b61d2deacc8f8e193d8fdf1628964db33805d9306386779cc668af9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11297555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00174787$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Martin, C C</creatorcontrib><creatorcontrib>Bischof, L J</creatorcontrib><creatorcontrib>Bergman, B</creatorcontrib><creatorcontrib>Hornbuckle, L A</creatorcontrib><creatorcontrib>Hilliker, C</creatorcontrib><creatorcontrib>Frigeri, C</creatorcontrib><creatorcontrib>Wahl, D</creatorcontrib><creatorcontrib>Svitek, C A</creatorcontrib><creatorcontrib>Wong, R</creatorcontrib><creatorcontrib>Goldman, J K</creatorcontrib><creatorcontrib>Oeser, J K</creatorcontrib><creatorcontrib>Leprêtre, F</creatorcontrib><creatorcontrib>Froguel, P</creatorcontrib><creatorcontrib>O'Brien, R M</creatorcontrib><creatorcontrib>Hutton, J C</creatorcontrib><title>Cloning and Characterization of the Human and Rat Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein (IGRP) Genes</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Islet-specific glucose-6-phosphatase (G6Pase) catalytic subunit-related protein (IGRP) is a homolog of the catalytic subunit
of G6Pase, the enzyme that catalyzes the terminal step of the gluconeogenic pathway. Its catalytic activity, however, has
not been defined. Since IGRP gene expression is restricted to islets, this suggests a possible role in the regulation of islet metabolism and, hence,
insulin secretion induced by metabolites. We report here a comparative analysis of the human, mouse, and rat IGRP genes. These studies aimed to identify conserved sequences that may be critical for IGRP function and that specify its restricted
tissue distribution. The single copy human IGRP gene has five exons of similar length and coding sequence to the mouse IGRP gene and is located on human chromosome 2q28â32 adjacent to the myosin heavy chain 1B gene. In contrast, the rat IGRP gene does not appear to encode a protein as a result of a series of deletions and insertions in the coding sequence. Moreover,
rat IGRP mRNA, unlike mouse and human IGRP mRNA, is not expressed in islets or islet-derived cell lines, an observation that
was traced by fusion gene analysis to a mutation of the TATA box motif in the mouse/human IGRP promoters to TGTA in the rat
sequence. The results provide a framework for the further analysis of the molecular basis for the tissue-restricted expression
of the IGRP gene and the identification of key amino acid sequences that determine its biological activity.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Catalytic Domain</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>Genetics</subject><subject>Glucose-6-Phosphatase</subject><subject>Glucose-6-Phosphatase - chemistry</subject><subject>Glucose-6-Phosphatase - genetics</subject><subject>Human genetics</subject><subject>Humans</subject><subject>IGRP gene</subject><subject>Immunoenzyme Techniques</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions (Genetics)</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger</subject><subject>RNA, Messenger - analysis</subject><subject>Sequence Alignment</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-L1DAUx4Mo7rh69Sg5iLiHjknT5sdxKTozMOKyKngLafq6zdI2Y5Iq693_26wzuEcfPB6893m_-CL0kpI1JaJ6d9va9UdKaF2pkpBHaEWJZAWr6bfHaEVISQtV1vIMPYvxlmSrFH2KzigtlajreoV-N6Of3XyDzdzhZjDB2ATB_TLJ-Rn7HqcB8HaZzPyXuDYJ7-IIqYgHsK53Fm_GxfoIBS8Og4-HwSQTATc5jHcp1z8v7TK7VAQYTYIOXwWfwM347W5zfXWBNzBDfI6e9GaM8OIUz9HXD--_NNti_2mzay73hWUVIYXh3CrBq5bTruzAWCt7CVSxTvZdT3kpFa-6ljFJ6k4xwpnkQihrOZemVz07RxfHuYMZ9SG4yYQ77Y3T28u9vs8RQkUlpPhBM_vmyB6C_75ATHpy0cI4mhn8ErUgSkiSl_8PpEIqWUqewfURtMHHGKD_dwIl-l5NndXUD2rmhlenyUs7QfeAn-TLwOvTP-5m-OkC6NZ5O8CkS8Gz67KmSrA_kaSl8A</recordid><startdate>20010706</startdate><enddate>20010706</enddate><creator>Martin, C C</creator><creator>Bischof, L J</creator><creator>Bergman, B</creator><creator>Hornbuckle, L A</creator><creator>Hilliker, C</creator><creator>Frigeri, C</creator><creator>Wahl, D</creator><creator>Svitek, C A</creator><creator>Wong, R</creator><creator>Goldman, J K</creator><creator>Oeser, J K</creator><creator>Leprêtre, F</creator><creator>Froguel, P</creator><creator>O'Brien, R M</creator><creator>Hutton, J C</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20010706</creationdate><title>Cloning and Characterization of the Human and Rat Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein (IGRP) Genes</title><author>Martin, C C ; Bischof, L J ; Bergman, B ; Hornbuckle, L A ; Hilliker, C ; Frigeri, C ; Wahl, D ; Svitek, C A ; Wong, R ; Goldman, J K ; Oeser, J K ; Leprêtre, F ; Froguel, P ; O'Brien, R M ; Hutton, J C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3400-a66c9764b61d2deacc8f8e193d8fdf1628964db33805d9306386779cc668af9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Catalytic Domain</topic><topic>Cloning, Molecular</topic><topic>Conserved Sequence</topic><topic>Genetics</topic><topic>Glucose-6-Phosphatase</topic><topic>Glucose-6-Phosphatase - chemistry</topic><topic>Glucose-6-Phosphatase - genetics</topic><topic>Human genetics</topic><topic>Humans</topic><topic>IGRP gene</topic><topic>Immunoenzyme Techniques</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions (Genetics)</topic><topic>Promoter Regions, Genetic</topic><topic>Proteins</topic><topic>Proteins - chemistry</topic><topic>Proteins - genetics</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger</topic><topic>RNA, Messenger - analysis</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martin, C C</creatorcontrib><creatorcontrib>Bischof, L J</creatorcontrib><creatorcontrib>Bergman, B</creatorcontrib><creatorcontrib>Hornbuckle, L A</creatorcontrib><creatorcontrib>Hilliker, C</creatorcontrib><creatorcontrib>Frigeri, C</creatorcontrib><creatorcontrib>Wahl, D</creatorcontrib><creatorcontrib>Svitek, C A</creatorcontrib><creatorcontrib>Wong, R</creatorcontrib><creatorcontrib>Goldman, J K</creatorcontrib><creatorcontrib>Oeser, J K</creatorcontrib><creatorcontrib>Leprêtre, F</creatorcontrib><creatorcontrib>Froguel, P</creatorcontrib><creatorcontrib>O'Brien, R M</creatorcontrib><creatorcontrib>Hutton, J C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martin, C C</au><au>Bischof, L J</au><au>Bergman, B</au><au>Hornbuckle, L A</au><au>Hilliker, C</au><au>Frigeri, C</au><au>Wahl, D</au><au>Svitek, C A</au><au>Wong, R</au><au>Goldman, J K</au><au>Oeser, J K</au><au>Leprêtre, F</au><au>Froguel, P</au><au>O'Brien, R M</au><au>Hutton, J C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Characterization of the Human and Rat Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein (IGRP) Genes</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2001-07-06</date><risdate>2001</risdate><volume>276</volume><issue>27</issue><spage>25197</spage><epage>25207</epage><pages>25197-25207</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Islet-specific glucose-6-phosphatase (G6Pase) catalytic subunit-related protein (IGRP) is a homolog of the catalytic subunit
of G6Pase, the enzyme that catalyzes the terminal step of the gluconeogenic pathway. Its catalytic activity, however, has
not been defined. Since IGRP gene expression is restricted to islets, this suggests a possible role in the regulation of islet metabolism and, hence,
insulin secretion induced by metabolites. We report here a comparative analysis of the human, mouse, and rat IGRP genes. These studies aimed to identify conserved sequences that may be critical for IGRP function and that specify its restricted
tissue distribution. The single copy human IGRP gene has five exons of similar length and coding sequence to the mouse IGRP gene and is located on human chromosome 2q28â32 adjacent to the myosin heavy chain 1B gene. In contrast, the rat IGRP gene does not appear to encode a protein as a result of a series of deletions and insertions in the coding sequence. Moreover,
rat IGRP mRNA, unlike mouse and human IGRP mRNA, is not expressed in islets or islet-derived cell lines, an observation that
was traced by fusion gene analysis to a mutation of the TATA box motif in the mouse/human IGRP promoters to TGTA in the rat
sequence. The results provide a framework for the further analysis of the molecular basis for the tissue-restricted expression
of the IGRP gene and the identification of key amino acid sequences that determine its biological activity.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>11297555</pmid><doi>10.1074/jbc.M101549200</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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ispartof | The Journal of biological chemistry, 2001-07, Vol.276 (27), p.25197-25207 |
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subjects | Amino Acid Sequence Animals Base Sequence Catalytic Domain Cloning, Molecular Conserved Sequence Genetics Glucose-6-Phosphatase Glucose-6-Phosphatase - chemistry Glucose-6-Phosphatase - genetics Human genetics Humans IGRP gene Immunoenzyme Techniques Life Sciences Mice Molecular Sequence Data Promoter Regions (Genetics) Promoter Regions, Genetic Proteins Proteins - chemistry Proteins - genetics Rats Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Messenger - analysis Sequence Alignment |
title | Cloning and Characterization of the Human and Rat Islet-specific Glucose-6-phosphatase Catalytic Subunit-related Protein (IGRP) Genes |
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