A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells

Members of the RUNX and GATA transcription factor families play critical roles during hematopoiesis from Drosophila to mammals. In Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its inte...

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Veröffentlicht in:Developmental biology 2007-05, Vol.305 (2), p.726-734
Hauptverfasser: Ferjoux, Géraldine, Augé, Benoit, Boyer, Karène, Haenlin, Marc, Waltzer, Lucas
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container_issue 2
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container_title Developmental biology
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creator Ferjoux, Géraldine
Augé, Benoit
Boyer, Karène
Haenlin, Marc
Waltzer, Lucas
description Members of the RUNX and GATA transcription factor families play critical roles during hematopoiesis from Drosophila to mammals. In Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its interaction with the GATA factor Serpent (Srp). Crystal cells are the main source of prophenoloxidases (proPOs), the enzymes required for melanization. By analyzing the promoter regions of several insect proPOs, we identify a conserved GATA/RUNX cis-regulatory module that ensures the crystal cell-specific expression of the three Drosophila melanogaster proPO. We demonstrate that activation of this module requires the direct binding of both Srp and Lz. Interestingly, a similar GATA/RUNX signature is over-represented in crystal cell differentiation markers, allowing us to identify new Srp/Lz target genes by genome-wide screening of Drosophila promoter regions. Finally, we show that the expression of lz in the crystal cells also relies on Srp/Lz-mediated activation via a similar module, indicating that crystal cell fate choice maintenance and activation of the differentiation program are coupled. Based on our observations, we propose that this GATA/RUNX cis-regulatory module may be reiteratively used during hematopoietic development through evolution.
doi_str_mv 10.1016/j.ydbio.2007.03.010
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In Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its interaction with the GATA factor Serpent (Srp). Crystal cells are the main source of prophenoloxidases (proPOs), the enzymes required for melanization. By analyzing the promoter regions of several insect proPOs, we identify a conserved GATA/RUNX cis-regulatory module that ensures the crystal cell-specific expression of the three Drosophila melanogaster proPO. We demonstrate that activation of this module requires the direct binding of both Srp and Lz. Interestingly, a similar GATA/RUNX signature is over-represented in crystal cell differentiation markers, allowing us to identify new Srp/Lz target genes by genome-wide screening of Drosophila promoter regions. Finally, we show that the expression of lz in the crystal cells also relies on Srp/Lz-mediated activation via a similar module, indicating that crystal cell fate choice maintenance and activation of the differentiation program are coupled. Based on our observations, we propose that this GATA/RUNX cis-regulatory module may be reiteratively used during hematopoietic development through evolution.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17418114</pmid><doi>10.1016/j.ydbio.2007.03.010</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5361-727X</orcidid><orcidid>https://orcid.org/0000-0003-2728-8457</orcidid><oa>free_for_read</oa></addata></record>
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subjects Animals
Biochemistry, Molecular Biology
Catechol Oxidase
Catechol Oxidase - genetics
Cell Differentiation
Cell Differentiation - physiology
Conserved Sequence
Core Binding Factor alpha Subunits
Core Binding Factor alpha Subunits - physiology
DNA-Binding Proteins
DNA-Binding Proteins - metabolism
DNA-Binding Proteins - physiology
Drosophila
Drosophila melanogaster
Drosophila melanogaster - cytology
Drosophila melanogaster - embryology
Drosophila melanogaster - enzymology
Drosophila melanogaster - growth & development
Drosophila Proteins
Drosophila Proteins - metabolism
Drosophila Proteins - physiology
Enzyme Precursors
Enzyme Precursors - genetics
GATA
GATA Transcription Factors
GATA Transcription Factors - metabolism
GATA Transcription Factors - physiology
Gene Expression Regulation, Developmental
Gene Expression Regulation, Developmental - physiology
Hematopoiesis
Hemocytes
Hemocytes - cytology
Larva
Larva - cytology
Larva - growth & development
Life Sciences
RUNX
Transcription
Transcription Factors
Transcription Factors - metabolism
Transcription Factors - physiology
title A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells
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