A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells
Members of the RUNX and GATA transcription factor families play critical roles during hematopoiesis from Drosophila to mammals. In Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its inte...
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description | Members of the RUNX and GATA transcription factor families play critical roles during hematopoiesis from
Drosophila to mammals. In
Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its interaction with the GATA factor Serpent (Srp). Crystal cells are the main source of prophenoloxidases (proPOs), the enzymes required for melanization. By analyzing the promoter regions of several insect
proPOs, we identify a conserved GATA/RUNX
cis-regulatory module that ensures the crystal cell-specific expression of the three
Drosophila melanogaster proPO. We demonstrate that activation of this module requires the direct binding of both Srp and Lz. Interestingly, a similar GATA/RUNX signature is over-represented in crystal cell differentiation markers, allowing us to identify new Srp/Lz target genes by genome-wide screening of
Drosophila promoter regions. Finally, we show that the expression of
lz in the crystal cells also relies on Srp/Lz-mediated activation via a similar module, indicating that crystal cell fate choice maintenance and activation of the differentiation program are coupled. Based on our observations, we propose that this GATA/RUNX
cis-regulatory module may be reiteratively used during hematopoietic development through evolution. |
doi_str_mv | 10.1016/j.ydbio.2007.03.010 |
format | Article |
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Drosophila to mammals. In
Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its interaction with the GATA factor Serpent (Srp). Crystal cells are the main source of prophenoloxidases (proPOs), the enzymes required for melanization. By analyzing the promoter regions of several insect
proPOs, we identify a conserved GATA/RUNX
cis-regulatory module that ensures the crystal cell-specific expression of the three
Drosophila melanogaster proPO. We demonstrate that activation of this module requires the direct binding of both Srp and Lz. Interestingly, a similar GATA/RUNX signature is over-represented in crystal cell differentiation markers, allowing us to identify new Srp/Lz target genes by genome-wide screening of
Drosophila promoter regions. Finally, we show that the expression of
lz in the crystal cells also relies on Srp/Lz-mediated activation via a similar module, indicating that crystal cell fate choice maintenance and activation of the differentiation program are coupled. Based on our observations, we propose that this GATA/RUNX
cis-regulatory module may be reiteratively used during hematopoietic development through evolution.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/j.ydbio.2007.03.010</identifier><identifier>PMID: 17418114</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Biochemistry, Molecular Biology ; Catechol Oxidase ; Catechol Oxidase - genetics ; Cell Differentiation ; Cell Differentiation - physiology ; Conserved Sequence ; Core Binding Factor alpha Subunits ; Core Binding Factor alpha Subunits - physiology ; DNA-Binding Proteins ; DNA-Binding Proteins - metabolism ; DNA-Binding Proteins - physiology ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - cytology ; Drosophila melanogaster - embryology ; Drosophila melanogaster - enzymology ; Drosophila melanogaster - growth & development ; Drosophila Proteins ; Drosophila Proteins - metabolism ; Drosophila Proteins - physiology ; Enzyme Precursors ; Enzyme Precursors - genetics ; GATA ; GATA Transcription Factors ; GATA Transcription Factors - metabolism ; GATA Transcription Factors - physiology ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Developmental - physiology ; Hematopoiesis ; Hemocytes ; Hemocytes - cytology ; Larva ; Larva - cytology ; Larva - growth & development ; Life Sciences ; RUNX ; Transcription ; Transcription Factors ; Transcription Factors - metabolism ; Transcription Factors - physiology</subject><ispartof>Developmental biology, 2007-05, Vol.305 (2), p.726-734</ispartof><rights>2007 Elsevier Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-943fa35ba754549ca7b05ee65dc5ad1781955a7f888c9347bc59160e0de09f733</citedby><cites>FETCH-LOGICAL-c467t-943fa35ba754549ca7b05ee65dc5ad1781955a7f888c9347bc59160e0de09f733</cites><orcidid>0000-0002-5361-727X ; 0000-0003-2728-8457</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ydbio.2007.03.010$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17418114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00170567$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferjoux, Géraldine</creatorcontrib><creatorcontrib>Augé, Benoit</creatorcontrib><creatorcontrib>Boyer, Karène</creatorcontrib><creatorcontrib>Haenlin, Marc</creatorcontrib><creatorcontrib>Waltzer, Lucas</creatorcontrib><title>A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Members of the RUNX and GATA transcription factor families play critical roles during hematopoiesis from
Drosophila to mammals. In
Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its interaction with the GATA factor Serpent (Srp). Crystal cells are the main source of prophenoloxidases (proPOs), the enzymes required for melanization. By analyzing the promoter regions of several insect
proPOs, we identify a conserved GATA/RUNX
cis-regulatory module that ensures the crystal cell-specific expression of the three
Drosophila melanogaster proPO. We demonstrate that activation of this module requires the direct binding of both Srp and Lz. Interestingly, a similar GATA/RUNX signature is over-represented in crystal cell differentiation markers, allowing us to identify new Srp/Lz target genes by genome-wide screening of
Drosophila promoter regions. Finally, we show that the expression of
lz in the crystal cells also relies on Srp/Lz-mediated activation via a similar module, indicating that crystal cell fate choice maintenance and activation of the differentiation program are coupled. Based on our observations, we propose that this GATA/RUNX
cis-regulatory module may be reiteratively used during hematopoietic development through evolution.</description><subject>Animals</subject><subject>Biochemistry, Molecular Biology</subject><subject>Catechol Oxidase</subject><subject>Catechol Oxidase - genetics</subject><subject>Cell Differentiation</subject><subject>Cell Differentiation - physiology</subject><subject>Conserved Sequence</subject><subject>Core Binding Factor alpha Subunits</subject><subject>Core Binding Factor alpha Subunits - physiology</subject><subject>DNA-Binding Proteins</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - cytology</subject><subject>Drosophila melanogaster - embryology</subject><subject>Drosophila melanogaster - enzymology</subject><subject>Drosophila melanogaster - growth & development</subject><subject>Drosophila Proteins</subject><subject>Drosophila Proteins - metabolism</subject><subject>Drosophila Proteins - physiology</subject><subject>Enzyme Precursors</subject><subject>Enzyme Precursors - genetics</subject><subject>GATA</subject><subject>GATA Transcription Factors</subject><subject>GATA Transcription Factors - metabolism</subject><subject>GATA Transcription Factors - physiology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Hematopoiesis</subject><subject>Hemocytes</subject><subject>Hemocytes - cytology</subject><subject>Larva</subject><subject>Larva - cytology</subject><subject>Larva - growth & development</subject><subject>Life Sciences</subject><subject>RUNX</subject><subject>Transcription</subject><subject>Transcription Factors</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Factors - physiology</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAUhC0EokvhFyAhn5A4JH1ex3Fy4BC10CKtQEKt1Jvl2C_UKyfe2kml_fd4uyu4wcmy_c3zeIaQ9wxKBqy-2JZ727tQrgFkCbwEBi_IikErClFX9y_JCoCtC1ZDfUbepLQFAN40_DU5Y7JiDWPVijx29Lq77S5-3n2_p8alIuKvxes5xD0dg108UhOWncdEr2JIYffgvKa9D8FSg97n23F084jTTPVkqXXDgDHvnJ5dmKib5kBN3KdZ-2dBekteDdonfHdaz8nd1y-3lzfF5sf1t8tuU5iqlnPRVnzQXPRaikpUrdGyB4FYC2uEtkw2rBVCy6FpGtPySvZGtPmrCBahHSTn5-TTce6D9moX3ajjXgXt1E23UYeznI4EUcsnltmPR3YXw-OCaVajSwe3esKwJCUhe6qzpf-BrJU1SFhnkB9Bk1NLEYc_FhioQ31qq57rU4f6FHCV68uqD6fxSz-i_as59ZWBz0cAc3JPDqNKxuFk0LqIZlY2uH8-8Bt0S6xR</recordid><startdate>20070515</startdate><enddate>20070515</enddate><creator>Ferjoux, Géraldine</creator><creator>Augé, Benoit</creator><creator>Boyer, Karène</creator><creator>Haenlin, Marc</creator><creator>Waltzer, Lucas</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5361-727X</orcidid><orcidid>https://orcid.org/0000-0003-2728-8457</orcidid></search><sort><creationdate>20070515</creationdate><title>A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells</title><author>Ferjoux, Géraldine ; Augé, Benoit ; Boyer, Karène ; Haenlin, Marc ; Waltzer, Lucas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-943fa35ba754549ca7b05ee65dc5ad1781955a7f888c9347bc59160e0de09f733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biochemistry, Molecular Biology</topic><topic>Catechol Oxidase</topic><topic>Catechol Oxidase - genetics</topic><topic>Cell Differentiation</topic><topic>Cell Differentiation - physiology</topic><topic>Conserved Sequence</topic><topic>Core Binding Factor alpha Subunits</topic><topic>Core Binding Factor alpha Subunits - physiology</topic><topic>DNA-Binding Proteins</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - cytology</topic><topic>Drosophila melanogaster - embryology</topic><topic>Drosophila melanogaster - enzymology</topic><topic>Drosophila melanogaster - growth & development</topic><topic>Drosophila Proteins</topic><topic>Drosophila Proteins - metabolism</topic><topic>Drosophila Proteins - physiology</topic><topic>Enzyme Precursors</topic><topic>Enzyme Precursors - genetics</topic><topic>GATA</topic><topic>GATA Transcription Factors</topic><topic>GATA Transcription Factors - metabolism</topic><topic>GATA Transcription Factors - physiology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Hematopoiesis</topic><topic>Hemocytes</topic><topic>Hemocytes - cytology</topic><topic>Larva</topic><topic>Larva - cytology</topic><topic>Larva - growth & development</topic><topic>Life Sciences</topic><topic>RUNX</topic><topic>Transcription</topic><topic>Transcription Factors</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Factors - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferjoux, Géraldine</creatorcontrib><creatorcontrib>Augé, Benoit</creatorcontrib><creatorcontrib>Boyer, Karène</creatorcontrib><creatorcontrib>Haenlin, Marc</creatorcontrib><creatorcontrib>Waltzer, Lucas</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferjoux, Géraldine</au><au>Augé, Benoit</au><au>Boyer, Karène</au><au>Haenlin, Marc</au><au>Waltzer, Lucas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2007-05-15</date><risdate>2007</risdate><volume>305</volume><issue>2</issue><spage>726</spage><epage>734</epage><pages>726-734</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Members of the RUNX and GATA transcription factor families play critical roles during hematopoiesis from
Drosophila to mammals. In
Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its interaction with the GATA factor Serpent (Srp). Crystal cells are the main source of prophenoloxidases (proPOs), the enzymes required for melanization. By analyzing the promoter regions of several insect
proPOs, we identify a conserved GATA/RUNX
cis-regulatory module that ensures the crystal cell-specific expression of the three
Drosophila melanogaster proPO. We demonstrate that activation of this module requires the direct binding of both Srp and Lz. Interestingly, a similar GATA/RUNX signature is over-represented in crystal cell differentiation markers, allowing us to identify new Srp/Lz target genes by genome-wide screening of
Drosophila promoter regions. Finally, we show that the expression of
lz in the crystal cells also relies on Srp/Lz-mediated activation via a similar module, indicating that crystal cell fate choice maintenance and activation of the differentiation program are coupled. Based on our observations, we propose that this GATA/RUNX
cis-regulatory module may be reiteratively used during hematopoietic development through evolution.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17418114</pmid><doi>10.1016/j.ydbio.2007.03.010</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5361-727X</orcidid><orcidid>https://orcid.org/0000-0003-2728-8457</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biochemistry, Molecular Biology Catechol Oxidase Catechol Oxidase - genetics Cell Differentiation Cell Differentiation - physiology Conserved Sequence Core Binding Factor alpha Subunits Core Binding Factor alpha Subunits - physiology DNA-Binding Proteins DNA-Binding Proteins - metabolism DNA-Binding Proteins - physiology Drosophila Drosophila melanogaster Drosophila melanogaster - cytology Drosophila melanogaster - embryology Drosophila melanogaster - enzymology Drosophila melanogaster - growth & development Drosophila Proteins Drosophila Proteins - metabolism Drosophila Proteins - physiology Enzyme Precursors Enzyme Precursors - genetics GATA GATA Transcription Factors GATA Transcription Factors - metabolism GATA Transcription Factors - physiology Gene Expression Regulation, Developmental Gene Expression Regulation, Developmental - physiology Hematopoiesis Hemocytes Hemocytes - cytology Larva Larva - cytology Larva - growth & development Life Sciences RUNX Transcription Transcription Factors Transcription Factors - metabolism Transcription Factors - physiology |
title | A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells |
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