CENP-A-containing Nucleosomes: Easier Disassembly versus Exclusive Centromeric Localization

CENP-A is a histone variant that replaces conventional H3 in nucleosomes of functional centromeres. We report here, from reconstitutions of CENP-A- and H3-containing nucleosomes on linear DNA fragments and the comparison of their electrophoretic mobility, that CENP-A induces some positioning of its...

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Veröffentlicht in:	Journal of molecular biology 2007-07, Vol.370 (3), p.555-573
Hauptverfasser:	Conde e Silva, Natalia, Black, Ben E., Sivolob, Andrei, Filipski, Jan, Cleveland, Don W., Prunell, Ariel
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Autoantigens - chemistry Autoantigens - genetics Autoantigens - metabolism Biochemistry, Molecular Biology Cell Cycle Proteins - metabolism CENP-A proteolysis CENP-A targeting Centromere - metabolism Centromere Protein A Chickens Chromosomal Proteins, Non-Histone - chemistry Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism Dimerization DNA - chemistry DNA - metabolism DNA topology Exodeoxyribonucleases - metabolism heparin Heparin - metabolism Histones - metabolism Humans Life Sciences Macromolecular Substances Models, Molecular Molecular biology Molecular Sequence Data NAP-1 Nuclear Proteins - metabolism Nucleic Acid Conformation Nucleosome Assembly Protein 1 Nucleosomes - chemistry Nucleosomes - metabolism Protein Conformation Sequence Alignment
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