Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa
FpvA, the pyoverdine outer‐membrane receptor from Pseudomonas aeruginosa, is involved in iron uptake when bacteria grow under iron limitation. Crystals of the in vivo pyoverdine‐loaded FpvA were obtained under several crystallization conditions using different detergents. A native data set was colle...
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| Veröffentlicht in: | Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2004-08, Vol.60 (8), p.1467-1469 |
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| container_title | Acta crystallographica. Section D, Biological crystallography. |
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| creator | Cobessi, David Célia, Hervé Folschweiller, Nicolas Heymann, Michael Schalk, Isabelle Abdallah, Mohamed Pattus, Franc |
| description | FpvA, the pyoverdine outer‐membrane receptor from Pseudomonas aeruginosa, is involved in iron uptake when bacteria grow under iron limitation. Crystals of the in vivo pyoverdine‐loaded FpvA were obtained under several crystallization conditions using different detergents. A native data set was collected at 3.6 Å resolution and a three‐wavelength MAD data set was collected at 3.6 Å resolution using crystals of selenomethionine‐substituted protein. The crystals grew under similar conditions and both belong to space group C2, but have different unit‐cell parameters. |
| doi_str_mv | 10.1107/S0907444904013496 |
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Section D, Biological crystallography.</title><addtitle>Acta Cryst. D</addtitle><description>FpvA, the pyoverdine outer‐membrane receptor from Pseudomonas aeruginosa, is involved in iron uptake when bacteria grow under iron limitation. Crystals of the in vivo pyoverdine‐loaded FpvA were obtained under several crystallization conditions using different detergents. A native data set was collected at 3.6 Å resolution and a three‐wavelength MAD data set was collected at 3.6 Å resolution using crystals of selenomethionine‐substituted protein. The crystals grew under similar conditions and both belong to space group C2, but have different unit‐cell parameters.</description><subject>Bacterial Outer Membrane Proteins</subject><subject>Bacterial Outer Membrane Proteins - chemistry</subject><subject>Bacterial Outer Membrane Proteins - metabolism</subject><subject>Biochemistry, Molecular Biology</subject><subject>Crystallization</subject><subject>Crystallography, X-Ray</subject><subject>FpvA</subject><subject>Life Sciences</subject><subject>Oligopeptides</subject><subject>Oligopeptides - metabolism</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - chemistry</subject><subject>Pseudomonas aeruginosa - cytology</subject><subject>pyoverdine receptor</subject><subject>Structural Biology</subject><issn>1399-0047</issn><issn>0907-4449</issn><issn>2059-7983</issn><issn>1399-0047</issn><issn>2059-7983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoqXwA7ggn5A4BMYfie3jakt3QSuo1CLgZHmTCTUkcbCThfDrcZVVQeLAyaPR87zy6M2ypxReUgry1RVokEIIDQIoF7q8l51SrnUOIOT9v-aT7FGMXwGAMS4fZie0YJJRqU-zeR3mONq2db_s6HxPbF-TIWDrOtfbMJNPebBz2tp2ji4S35DxBomfRgykw24fbI9kmP0BQ-3SGLDCYfSBXAyHFWmC78hlxKn2ne9tJBbD9MX1PtrH2YPGthGfHN-z7MPF6-v1Nt-937xZr3Z5JagSeVE1pSwU1I0uhag0U7yiiFwJzWQJOh0vldqXwJStBVSqpGJPk6kKa5kEfpa9WHJvbGuG4Lp0lfHWme1qZ253ALSglPMDTezzhR2C_z5hHE3nYoVtm470UzRlKTlVWiWQLmAVfIwBm7tkCua2G_NPN8l5dgyf9h3Wf4xjGQlQC_DDtTj_P9GsPp9fXxXARFLzRXVxxJ93qg3fTPqyLMzHdxtzuV1v1Prt1jD-G-qsqHU</recordid><startdate>200408</startdate><enddate>200408</enddate><creator>Cobessi, David</creator><creator>Célia, Hervé</creator><creator>Folschweiller, Nicolas</creator><creator>Heymann, Michael</creator><creator>Schalk, Isabelle</creator><creator>Abdallah, Mohamed</creator><creator>Pattus, Franc</creator><general>Munksgaard International Publishers</general><general>International Union of Crystallography</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5103-7976</orcidid><orcidid>https://orcid.org/0000-0002-8351-1679</orcidid></search><sort><creationdate>200408</creationdate><title>Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa</title><author>Cobessi, David ; Célia, Hervé ; Folschweiller, Nicolas ; Heymann, Michael ; Schalk, Isabelle ; Abdallah, Mohamed ; Pattus, Franc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4184-5cf67580df9644c9283c1ee384927609490788b6028ad40c8614b118485aa2703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Bacterial Outer Membrane Proteins</topic><topic>Bacterial Outer Membrane Proteins - chemistry</topic><topic>Bacterial Outer Membrane Proteins - metabolism</topic><topic>Biochemistry, Molecular Biology</topic><topic>Crystallization</topic><topic>Crystallography, X-Ray</topic><topic>FpvA</topic><topic>Life Sciences</topic><topic>Oligopeptides</topic><topic>Oligopeptides - metabolism</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - chemistry</topic><topic>Pseudomonas aeruginosa - cytology</topic><topic>pyoverdine receptor</topic><topic>Structural Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cobessi, David</creatorcontrib><creatorcontrib>Célia, Hervé</creatorcontrib><creatorcontrib>Folschweiller, Nicolas</creatorcontrib><creatorcontrib>Heymann, Michael</creatorcontrib><creatorcontrib>Schalk, Isabelle</creatorcontrib><creatorcontrib>Abdallah, Mohamed</creatorcontrib><creatorcontrib>Pattus, Franc</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Acta crystallographica. Section D, Biological crystallography.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cobessi, David</au><au>Célia, Hervé</au><au>Folschweiller, Nicolas</au><au>Heymann, Michael</au><au>Schalk, Isabelle</au><au>Abdallah, Mohamed</au><au>Pattus, Franc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa</atitle><jtitle>Acta crystallographica. Section D, Biological crystallography.</jtitle><addtitle>Acta Cryst. D</addtitle><date>2004-08</date><risdate>2004</risdate><volume>60</volume><issue>8</issue><spage>1467</spage><epage>1469</epage><pages>1467-1469</pages><issn>1399-0047</issn><issn>0907-4449</issn><issn>2059-7983</issn><eissn>1399-0047</eissn><eissn>2059-7983</eissn><abstract>FpvA, the pyoverdine outer‐membrane receptor from Pseudomonas aeruginosa, is involved in iron uptake when bacteria grow under iron limitation. Crystals of the in vivo pyoverdine‐loaded FpvA were obtained under several crystallization conditions using different detergents. A native data set was collected at 3.6 Å resolution and a three‐wavelength MAD data set was collected at 3.6 Å resolution using crystals of selenomethionine‐substituted protein. 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| ispartof | Acta crystallographica. Section D, Biological crystallography., 2004-08, Vol.60 (8), p.1467-1469 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Bacterial Outer Membrane Proteins Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - metabolism Biochemistry, Molecular Biology Crystallization Crystallography, X-Ray FpvA Life Sciences Oligopeptides Oligopeptides - metabolism Pseudomonas aeruginosa Pseudomonas aeruginosa - chemistry Pseudomonas aeruginosa - cytology pyoverdine receptor Structural Biology |
| title | Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa |
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