Phycobilisome core mutants of Synechocystis PCC 6803
Mutant strains of the cyanobacterium Synechocystis 6803 were constructed in which either the apcABC operon, encoding core subunits allophycocyanin α and β and a small linker L C 8, or th0e apcE gene encoding the phycobilisome core-membrane linker was deleted. Phycobilisome assembly and energy transf...
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| Veröffentlicht in: | Biochimica et biophysica acta. Bioenergetics 1995-09, Vol.1231 (2), p.189-196 |
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| container_title | Biochimica et biophysica acta. Bioenergetics |
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| creator | Ajlani, Ghada Vernotte, Claudie DiMagno, Lisa Haselkorn, Robert |
| description | Mutant strains of the cyanobacterium
Synechocystis 6803 were constructed in which either the
apcABC operon, encoding core subunits allophycocyanin α and β and a small linker L
C
8, or th0e
apcE gene encoding the phycobilisome core-membrane linker was deleted. Phycobilisome assembly and energy transfer were studied in these mutants using both SDS gel analysis of phycobiliprotein complexes and low temperature fluorescence spectroscopy. Both mutants assembled phycocyanin rods but neither assembled a core complex. Although the mutants have no functional phycobilisomes, they grow photoautotrophically. No energy transfer between the remaining soluble phycobiliproteins and the photosystems was observed. |
| doi_str_mv | 10.1016/0005-2728(95)00086-X |
| format | Article |
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Synechocystis 6803 were constructed in which either the
apcABC operon, encoding core subunits allophycocyanin α and β and a small linker L
C
8, or th0e
apcE gene encoding the phycobilisome core-membrane linker was deleted. Phycobilisome assembly and energy transfer were studied in these mutants using both SDS gel analysis of phycobiliprotein complexes and low temperature fluorescence spectroscopy. Both mutants assembled phycocyanin rods but neither assembled a core complex. Although the mutants have no functional phycobilisomes, they grow photoautotrophically. No energy transfer between the remaining soluble phycobiliproteins and the photosystems was observed.</description><identifier>ISSN: 0005-2728</identifier><identifier>EISSN: 1879-2650</identifier><identifier>DOI: 10.1016/0005-2728(95)00086-X</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Allophycocyanin ; Bacteriology ; Cyanobacterium ; Energy transfer ; Genetics ; L CM Photosystem ; Life Sciences ; Microbiology and Parasitology ; Phycobiliprotein</subject><ispartof>Biochimica et biophysica acta. Bioenergetics, 1995-09, Vol.1231 (2), p.189-196</ispartof><rights>1995</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-45ef9752b296c7800353325559e6e6acc37a93268ba6154e7d29f29a7bb099923</citedby><cites>FETCH-LOGICAL-c450t-45ef9752b296c7800353325559e6e6acc37a93268ba6154e7d29f29a7bb099923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0005-2728(95)00086-X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://hal.science/hal-00119404$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ajlani, Ghada</creatorcontrib><creatorcontrib>Vernotte, Claudie</creatorcontrib><creatorcontrib>DiMagno, Lisa</creatorcontrib><creatorcontrib>Haselkorn, Robert</creatorcontrib><title>Phycobilisome core mutants of Synechocystis PCC 6803</title><title>Biochimica et biophysica acta. Bioenergetics</title><description>Mutant strains of the cyanobacterium
Synechocystis 6803 were constructed in which either the
apcABC operon, encoding core subunits allophycocyanin α and β and a small linker L
C
8, or th0e
apcE gene encoding the phycobilisome core-membrane linker was deleted. Phycobilisome assembly and energy transfer were studied in these mutants using both SDS gel analysis of phycobiliprotein complexes and low temperature fluorescence spectroscopy. Both mutants assembled phycocyanin rods but neither assembled a core complex. Although the mutants have no functional phycobilisomes, they grow photoautotrophically. No energy transfer between the remaining soluble phycobiliproteins and the photosystems was observed.</description><subject>Allophycocyanin</subject><subject>Bacteriology</subject><subject>Cyanobacterium</subject><subject>Energy transfer</subject><subject>Genetics</subject><subject>L CM Photosystem</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>Phycobiliprotein</subject><issn>0005-2728</issn><issn>1879-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9kE1Lw0AQhhdRsFb_gYcc7SE6-529CCVYKxQsqNDbstlO6ErblWws5N-bWOnR0zDD-7wwDyG3FO4pUPUAADJnmhV3Rk76pVD56oyMaKFNzpSEczI6RS7JVUqf0GOC8RERy03nYxW2IcUdZj42mO2-W7dvUxbr7K3bo99E36U2pGxZlpkqgF-Ti9ptE978zTH5mD29l_N88fr8Uk4XuRcS2lxIrI2WrGJGeV0AcMk5k1IaVKic91w7w5kqKqeoFKjXzNTMOF1VYIxhfEwmx96N29qvJuxc09nogp1PF3a49W9QI0AcaJ8Vx6xvYkoN1ieAgh0s2UGBHRRYI-2vJbvqsccjhv0fh4CNTT7g3uM6NOhbu47h_4IfdmdrJA</recordid><startdate>19950912</startdate><enddate>19950912</enddate><creator>Ajlani, Ghada</creator><creator>Vernotte, Claudie</creator><creator>DiMagno, Lisa</creator><creator>Haselkorn, Robert</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope></search><sort><creationdate>19950912</creationdate><title>Phycobilisome core mutants of Synechocystis PCC 6803</title><author>Ajlani, Ghada ; Vernotte, Claudie ; DiMagno, Lisa ; Haselkorn, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-45ef9752b296c7800353325559e6e6acc37a93268ba6154e7d29f29a7bb099923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Allophycocyanin</topic><topic>Bacteriology</topic><topic>Cyanobacterium</topic><topic>Energy transfer</topic><topic>Genetics</topic><topic>L CM Photosystem</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>Phycobiliprotein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ajlani, Ghada</creatorcontrib><creatorcontrib>Vernotte, Claudie</creatorcontrib><creatorcontrib>DiMagno, Lisa</creatorcontrib><creatorcontrib>Haselkorn, Robert</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Biochimica et biophysica acta. Bioenergetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ajlani, Ghada</au><au>Vernotte, Claudie</au><au>DiMagno, Lisa</au><au>Haselkorn, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phycobilisome core mutants of Synechocystis PCC 6803</atitle><jtitle>Biochimica et biophysica acta. Bioenergetics</jtitle><date>1995-09-12</date><risdate>1995</risdate><volume>1231</volume><issue>2</issue><spage>189</spage><epage>196</epage><pages>189-196</pages><issn>0005-2728</issn><eissn>1879-2650</eissn><abstract>Mutant strains of the cyanobacterium
Synechocystis 6803 were constructed in which either the
apcABC operon, encoding core subunits allophycocyanin α and β and a small linker L
C
8, or th0e
apcE gene encoding the phycobilisome core-membrane linker was deleted. Phycobilisome assembly and energy transfer were studied in these mutants using both SDS gel analysis of phycobiliprotein complexes and low temperature fluorescence spectroscopy. Both mutants assembled phycocyanin rods but neither assembled a core complex. Although the mutants have no functional phycobilisomes, they grow photoautotrophically. No energy transfer between the remaining soluble phycobiliproteins and the photosystems was observed.</abstract><pub>Elsevier B.V</pub><doi>10.1016/0005-2728(95)00086-X</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochimica et biophysica acta. Bioenergetics, 1995-09, Vol.1231 (2), p.189-196 |
| issn | 0005-2728 1879-2650 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00119404v1 |
| source | Elsevier ScienceDirect Journals; EZB Electronic Journals Library |
| subjects | Allophycocyanin Bacteriology Cyanobacterium Energy transfer Genetics L CM Photosystem Life Sciences Microbiology and Parasitology Phycobiliprotein |
| title | Phycobilisome core mutants of Synechocystis PCC 6803 |
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