Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803
A mutant strain of the cyanobacterium Synechocystis PCC 6803, called PAL, (PC-, delta apc AB, delta apc E), lacking phycocyanin, allophycocyanin and the core-membrane linker (Lcm), was constructed. The strain was characterized by absorption and fluorescence spectroscopy. The mutant compensates for t...
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Veröffentlicht in: | Plant molecular biology 1998-06, Vol.37 (3), p.577-580 |
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description | A mutant strain of the cyanobacterium Synechocystis PCC 6803, called PAL, (PC-, delta apc AB, delta apc E), lacking phycocyanin, allophycocyanin and the core-membrane linker (Lcm), was constructed. The strain was characterized by absorption and fluorescence spectroscopy. The mutant compensates for the absence of the major PS II antenna by increasing its PS II / PS I ratio. It is stable and grows well albeit more slowly than wild type. |
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It is stable and grows well albeit more slowly than wild type.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/a:1005924730298</identifier><identifier>PMID: 9617824</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>absorbance ; allophycocyanin ; Bacteria ; Bacterial Proteins ; Bacterial Proteins - genetics ; Bacteriology ; Construction ; Cyanobacteria ; Cyanobacteria - genetics ; Cyanobacteria - growth & development ; DNA, Bacterial ; fluorescence ; Freshwater ; Genetics ; growth rate ; Life Sciences ; Light-Harvesting Protein Complexes ; Microbiology and Parasitology ; mutagenesis ; mutants ; Mutation ; photosystem I ; photosystem II ; phycocyanin ; pigments ; Plant Proteins ; Plant Proteins - genetics ; ratios ; Research Support, Non-U.S. Gov't ; spectral analysis ; Spectrum Analysis ; Synechocystis</subject><ispartof>Plant molecular biology, 1998-06, Vol.37 (3), p.577-580</ispartof><rights>Kluwer Academic Publishers 1998</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-fde8ee89b9a610fbe13dc6907ec8991ceca0b9e91b0f0b02f4c834c4c2e20c353</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9617824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00119402$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ajlani, G</creatorcontrib><creatorcontrib>Vernotte, C</creatorcontrib><title>Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>A mutant strain of the cyanobacterium Synechocystis PCC 6803, called PAL, (PC-, delta apc AB, delta apc E), lacking phycocyanin, allophycocyanin and the core-membrane linker (Lcm), was constructed. The strain was characterized by absorption and fluorescence spectroscopy. The mutant compensates for the absence of the major PS II antenna by increasing its PS II / PS I ratio. It is stable and grows well albeit more slowly than wild type.</description><subject>absorbance</subject><subject>allophycocyanin</subject><subject>Bacteria</subject><subject>Bacterial Proteins</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Construction</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - growth & development</subject><subject>DNA, Bacterial</subject><subject>fluorescence</subject><subject>Freshwater</subject><subject>Genetics</subject><subject>growth rate</subject><subject>Life Sciences</subject><subject>Light-Harvesting Protein Complexes</subject><subject>Microbiology and Parasitology</subject><subject>mutagenesis</subject><subject>mutants</subject><subject>Mutation</subject><subject>photosystem I</subject><subject>photosystem II</subject><subject>phycocyanin</subject><subject>pigments</subject><subject>Plant Proteins</subject><subject>Plant Proteins - genetics</subject><subject>ratios</subject><subject>Research Support, Non-U.S. Gov't</subject><subject>spectral analysis</subject><subject>Spectrum Analysis</subject><subject>Synechocystis</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0U1r3DAQBmBRWtJN0nNOpaaHQA5ORh_WR27BNElhoYU0ZzHWylkFr7W15ML211fpLj3k0pNg5kGjV0PIGYVLCoxf4TUFaAwTigMz-g1Z0EbxugGm35IFUKlqISh7T45TegYomMsjcmQkVZqJBcE2jilPs8shjhWOq8qtcUKX_RR-499i7Custuudi10YwnaK2YexHnxK1WbOOOYX8bAbvVtHt0s5pCptL6vvbVtJDfyUvOtxSP7D4Twhj7dffrT39fLb3df2Zlk7wUWu-5XX3mvTGZQU-s5TvnLSgPJOG0Oddwid8YZ20EMHrBdOc-GEY56B4w0_IRf7e9c42O0UNjjtbMRg72-W9qVW0lMjgP2ixZ7vbUnzc_Yp201Izg8Djj7OyaoysRGq-S-kUjAof1ng51fwOc7TWAJbJU2Za6Qp6OMBzd3Gr_698rCN0v-07_cYLT5NIdnHBwa0rFYbqgznfwCO6ZQP</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Ajlani, G</creator><creator>Vernotte, C</creator><general>Springer Nature B.V</general><general>Springer Verlag (Germany)</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>19980601</creationdate><title>Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. 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PCC 6803</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>37</volume><issue>3</issue><spage>577</spage><epage>580</epage><pages>577-580</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>A mutant strain of the cyanobacterium Synechocystis PCC 6803, called PAL, (PC-, delta apc AB, delta apc E), lacking phycocyanin, allophycocyanin and the core-membrane linker (Lcm), was constructed. The strain was characterized by absorption and fluorescence spectroscopy. The mutant compensates for the absence of the major PS II antenna by increasing its PS II / PS I ratio. It is stable and grows well albeit more slowly than wild type.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>9617824</pmid><doi>10.1023/a:1005924730298</doi><tpages>4</tpages></addata></record> |
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subjects | absorbance allophycocyanin Bacteria Bacterial Proteins Bacterial Proteins - genetics Bacteriology Construction Cyanobacteria Cyanobacteria - genetics Cyanobacteria - growth & development DNA, Bacterial fluorescence Freshwater Genetics growth rate Life Sciences Light-Harvesting Protein Complexes Microbiology and Parasitology mutagenesis mutants Mutation photosystem I photosystem II phycocyanin pigments Plant Proteins Plant Proteins - genetics ratios Research Support, Non-U.S. Gov't spectral analysis Spectrum Analysis Synechocystis |
title | Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803 |
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