Molecular analysis of psbA mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714
Mutations conferring herbicide resistance in 3 mutant strains of the cyanobacterium Synechocystis 6714 have been characterized by gene cloning and sequencing. The mutants display very different phenotypes: DCMU-IIA is DCMU-resistant and atrazine-resistant, DCMU-IIB is DCMU-resistant and atrazine-sen...
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| Veröffentlicht in: | Plant molecular biology 1989-11, Vol.13 (5), p.469-479 |
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| creator | AJLANI, G KIRILOVSKY, D PICAUD, M ASTIER, C |
| description | Mutations conferring herbicide resistance in 3 mutant strains of the cyanobacterium Synechocystis 6714 have been characterized by gene cloning and sequencing. The mutants display very different phenotypes: DCMU-IIA is DCMU-resistant and atrazine-resistant, DCMU-IIB is DCMU-resistant and atrazine-sensitive, and Az-V is DCMU-sensitive, atrazine-resistant and presents particular photoinhibition properties. These mutants were originally obtained either by one-step selection (DCMU-IIA) or by two-step selection (DCMU-IIB and Az-V). psbA copies carrying herbicide resistance have been identified by transformation experiments as psb AI in all cases. Sequences of the psb AI copy of each mutant have been compared to the wild-type sequence. In the single mutant DCMU-IIA, a point mutation at codon 264 (Ser---Ala) results in resistance to both DCMU and atrazine. In the double mutants DCMU-IIB and Az-V, two point mutations were found. DCMU-IIB was derived from DCMU-IIA and had acquired a second mutation at codon 255 (Phe---Leu) resulting in a slight increase in DCMU resistance and complete abolition of atrazine resistance. Az-V contains two changes at codons 211 (Phe---Ser) and 251 (Ala---Val) resulting in high atrazine resistance but only slight DCMU resistance. |
| doi_str_mv | 10.1007/BF00027307 |
| format | Article |
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The mutants display very different phenotypes: DCMU-IIA is DCMU-resistant and atrazine-resistant, DCMU-IIB is DCMU-resistant and atrazine-sensitive, and Az-V is DCMU-sensitive, atrazine-resistant and presents particular photoinhibition properties. These mutants were originally obtained either by one-step selection (DCMU-IIA) or by two-step selection (DCMU-IIB and Az-V). psbA copies carrying herbicide resistance have been identified by transformation experiments as psb AI in all cases. Sequences of the psb AI copy of each mutant have been compared to the wild-type sequence. In the single mutant DCMU-IIA, a point mutation at codon 264 (Ser---Ala) results in resistance to both DCMU and atrazine. In the double mutants DCMU-IIB and Az-V, two point mutations were found. DCMU-IIB was derived from DCMU-IIA and had acquired a second mutation at codon 255 (Phe---Leu) resulting in a slight increase in DCMU resistance and complete abolition of atrazine resistance. Az-V contains two changes at codons 211 (Phe---Ser) and 251 (Ala---Val) resulting in high atrazine resistance but only slight DCMU resistance.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00027307</identifier><identifier>PMID: 2518834</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Amino Acid Sequence ; Atrazine ; Atrazine - pharmacology ; Bacteriology ; Base Sequence ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Cloning, Molecular ; Cyanobacteria ; Cyanobacteria - drug effects ; Cyanobacteria - genetics ; Diuron ; Diuron - pharmacology ; DNA, Bacterial ; DNA, Bacterial - genetics ; Drug Resistance, Microbial ; Drug Resistance, Microbial - genetics ; Fundamental and applied biological sciences. Psychology ; Herbicides ; Herbicides - pharmacology ; Life Sciences ; Microbiology and Parasitology ; Molecular and cellular biology ; Molecular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis. Repair ; Mutation ; Phenotype ; Photosynthesis ; Photosynthesis - genetics ; Photosynthetic Reaction Center Complex Proteins ; Photosynthetic Reaction Center Complex Proteins - genetics ; Research Support, Non-U.S. Gov't ; Vegetal Biology</subject><ispartof>Plant molecular biology, 1989-11, Vol.13 (5), p.469-479</ispartof><rights>1990 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6655794$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2518834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00119399$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>AJLANI, G</creatorcontrib><creatorcontrib>KIRILOVSKY, D</creatorcontrib><creatorcontrib>PICAUD, M</creatorcontrib><creatorcontrib>ASTIER, C</creatorcontrib><title>Molecular analysis of psbA mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Mutations conferring herbicide resistance in 3 mutant strains of the cyanobacterium Synechocystis 6714 have been characterized by gene cloning and sequencing. The mutants display very different phenotypes: DCMU-IIA is DCMU-resistant and atrazine-resistant, DCMU-IIB is DCMU-resistant and atrazine-sensitive, and Az-V is DCMU-sensitive, atrazine-resistant and presents particular photoinhibition properties. These mutants were originally obtained either by one-step selection (DCMU-IIA) or by two-step selection (DCMU-IIB and Az-V). psbA copies carrying herbicide resistance have been identified by transformation experiments as psb AI in all cases. Sequences of the psb AI copy of each mutant have been compared to the wild-type sequence. In the single mutant DCMU-IIA, a point mutation at codon 264 (Ser---Ala) results in resistance to both DCMU and atrazine. In the double mutants DCMU-IIB and Az-V, two point mutations were found. DCMU-IIB was derived from DCMU-IIA and had acquired a second mutation at codon 255 (Phe---Leu) resulting in a slight increase in DCMU resistance and complete abolition of atrazine resistance. Az-V contains two changes at codons 211 (Phe---Ser) and 251 (Ala---Val) resulting in high atrazine resistance but only slight DCMU resistance.</description><subject>Amino Acid Sequence</subject><subject>Atrazine</subject><subject>Atrazine - pharmacology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - drug effects</subject><subject>Cyanobacteria - genetics</subject><subject>Diuron</subject><subject>Diuron - pharmacology</subject><subject>DNA, Bacterial</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Microbial</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herbicides</subject><subject>Herbicides - pharmacology</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>Molecular and cellular biology</subject><subject>Molecular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis. Repair</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Photosynthesis</subject><subject>Photosynthesis - genetics</subject><subject>Photosynthetic Reaction Center Complex Proteins</subject><subject>Photosynthetic Reaction Center Complex Proteins - genetics</subject><subject>Research Support, Non-U.S. Gov't</subject><subject>Vegetal Biology</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo90M9LwzAUB_AgypzTi3chBxE8VPOrTXucwzlh4sHdS5q90kjW1KQd9L83Y2OnB-99-IZ8Ebqn5IUSIl_floQQJjmRF2hKU8mTlLD8Ek0JzWQiBGXX6CaEX0Ii59kETVhK85yLKfJfzoIerPJYtcqOwQTsatyFao53Q69649qAPYQuTlNZwLXzeK-8cUPADfjKaLOFgzChV60G3DXQun7sIGDT4p-xBd04PYY-RmeSilt0VSsb4O40Z2izfN8sVsn6--NzMV8nDeOsT3JKBBN1JjIGNclpKmgF21yoQmu25YWSrI5Q5ZxWjAhNJNeiYjQTQIqc8hl6PsY2ypadNzvlx9IpU67m6_Kwi23QghfF_mCfjrbz7m-A0Jc7EzRYq1qI_ywPj6es4BE-nOBQ7WB7zj31Ge-Pp7sKWtnax0ZMOLMsS1NZCP4P9o2Cig</recordid><startdate>19891101</startdate><enddate>19891101</enddate><creator>AJLANI, G</creator><creator>KIRILOVSKY, D</creator><creator>PICAUD, M</creator><creator>ASTIER, C</creator><general>Springer</general><general>Springer Verlag (Germany)</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>1XC</scope></search><sort><creationdate>19891101</creationdate><title>Molecular analysis of psbA mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714</title><author>AJLANI, G ; KIRILOVSKY, D ; PICAUD, M ; ASTIER, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h232t-810424f6462ef081541bed84a9cc2d39a72f232a831b204c073c4b2164e09813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Atrazine</topic><topic>Atrazine - pharmacology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - drug effects</topic><topic>Cyanobacteria - genetics</topic><topic>Diuron</topic><topic>Diuron - pharmacology</topic><topic>DNA, Bacterial</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Microbial</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herbicides</topic><topic>Herbicides - pharmacology</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>Molecular and cellular biology</topic><topic>Molecular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis. Repair</topic><topic>Mutation</topic><topic>Phenotype</topic><topic>Photosynthesis</topic><topic>Photosynthesis - genetics</topic><topic>Photosynthetic Reaction Center Complex Proteins</topic><topic>Photosynthetic Reaction Center Complex Proteins - genetics</topic><topic>Research Support, Non-U.S. Gov't</topic><topic>Vegetal Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AJLANI, G</creatorcontrib><creatorcontrib>KIRILOVSKY, D</creatorcontrib><creatorcontrib>PICAUD, M</creatorcontrib><creatorcontrib>ASTIER, C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AJLANI, G</au><au>KIRILOVSKY, D</au><au>PICAUD, M</au><au>ASTIER, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of psbA mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1989-11-01</date><risdate>1989</risdate><volume>13</volume><issue>5</issue><spage>469</spage><epage>479</epage><pages>469-479</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>Mutations conferring herbicide resistance in 3 mutant strains of the cyanobacterium Synechocystis 6714 have been characterized by gene cloning and sequencing. The mutants display very different phenotypes: DCMU-IIA is DCMU-resistant and atrazine-resistant, DCMU-IIB is DCMU-resistant and atrazine-sensitive, and Az-V is DCMU-sensitive, atrazine-resistant and presents particular photoinhibition properties. These mutants were originally obtained either by one-step selection (DCMU-IIA) or by two-step selection (DCMU-IIB and Az-V). psbA copies carrying herbicide resistance have been identified by transformation experiments as psb AI in all cases. Sequences of the psb AI copy of each mutant have been compared to the wild-type sequence. In the single mutant DCMU-IIA, a point mutation at codon 264 (Ser---Ala) results in resistance to both DCMU and atrazine. In the double mutants DCMU-IIB and Az-V, two point mutations were found. DCMU-IIB was derived from DCMU-IIA and had acquired a second mutation at codon 255 (Phe---Leu) resulting in a slight increase in DCMU resistance and complete abolition of atrazine resistance. Az-V contains two changes at codons 211 (Phe---Ser) and 251 (Ala---Val) resulting in high atrazine resistance but only slight DCMU resistance.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>2518834</pmid><doi>10.1007/BF00027307</doi><tpages>11</tpages></addata></record> |
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| subjects | Amino Acid Sequence Atrazine Atrazine - pharmacology Bacteriology Base Sequence Biochemistry, Molecular Biology Biological and medical sciences Cloning, Molecular Cyanobacteria Cyanobacteria - drug effects Cyanobacteria - genetics Diuron Diuron - pharmacology DNA, Bacterial DNA, Bacterial - genetics Drug Resistance, Microbial Drug Resistance, Microbial - genetics Fundamental and applied biological sciences. Psychology Herbicides Herbicides - pharmacology Life Sciences Microbiology and Parasitology Molecular and cellular biology Molecular biology Molecular genetics Molecular Sequence Data Mutagenesis. Repair Mutation Phenotype Photosynthesis Photosynthesis - genetics Photosynthetic Reaction Center Complex Proteins Photosynthetic Reaction Center Complex Proteins - genetics Research Support, Non-U.S. Gov't Vegetal Biology |
| title | Molecular analysis of psbA mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714 |
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