Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations
Cynomolgus monkey is one of the macaque species currently used as an animal model for experimental surgery and medicine, in particular, to experiment new drugs or therapy protocols designed for the prevention of allograft rejection. In this field, it is of utmost importance to select histoincompatib...
Gespeichert in:
| Veröffentlicht in: | Immunogenetics (New York) 2006-05, Vol.58 (4), p.269-282 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 282 |
|---|---|
| container_issue | 4 |
| container_start_page | 269 |
| container_title | Immunogenetics (New York) |
| container_volume | 58 |
| creator | Blancher, Antoine Tisseyre, Pierre Dutaur, Marianne Apoil, Pol-André Maurer, Claudine Quesniaux, Valérie Raulf, Friedrich Bigaud, Marc Abbal, Michel |
| description | Cynomolgus monkey is one of the macaque species currently used as an animal model for experimental surgery and medicine, in particular, to experiment new drugs or therapy protocols designed for the prevention of allograft rejection. In this field, it is of utmost importance to select histoincompatible recipient-donor pairs. One way to ensure incompatibility between donor and recipient is to check their major histocompatibility complex (MHC) genotypes at the loci playing a determinant role in histocompatibility. We report in this paper on the cynomolgus monkey DRB polymorphism evidenced by sequencing of amplified exon 2 separated either by denaturing gradient gel electrophoresis (DGGE), or by cloning. By the study of 253 unrelated animals from two populations (Mauritius and The Philippines), we characterized 50 exon 2 sequences among which 28 were identical to sequences already reported in Macaca fascicularis or other macaque species (Macaca mulatta, Macaca nemestrina). By cloning and sequencing DRB cDNA, we revealed two additional DRB alleles. Out of the 20 haplotypes that we defined here, only two were found in both populations. The functional impact of DR incompatibility was studied in vitro by mixed lymphocyte culture. |
| doi_str_mv | 10.1007/s00251-006-0102-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_00094881v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2087443501</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-1b60e9650b65e728d903e7b1df58186a449fe63f526bd349dd657f7e2a2297fe3</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EokvhB3BBFgfEHlxm7MSOj2X5aKWtKvFxtpzEZlOSOI0Tqvx7vMoKJC7IB1vjZ17NvC8hLxEuEEC9iwA8RwYgGSBwph-RDWaCM-SIj8kGQAumFOIZeRbjHQDmmsun5AxlrrjguCHl12muFxo83S196EL7Y460C_1Pt9C3N7ZKh3obq6aaWzs2cUtvDtWHL--Pn96y9NrSIbRLF8bh0MSONj2dHkKqDalhakIfn5Mn3rbRvTjd5-T7p4_fdldsf_v5ene5Z5XQODEsJTgtcyhl7hQvag3CqRJrnxdYSJtl2jspfM5lWYtM13VawivHLedaeSfOyXbVPdjWDGPT2XExwTbm6nJvjjVIfmRFgb8wsW9WdhjD_eziZLomVq5tbe_CHI1UGvLk139BDlmRXC0S-Pof8C7MY58WNpKDksWqhitUjSHG0fk_cyKYY6RmjTSNKs0xUqNTz6uT8Fx2rv7bccpQ_Aae_5ks</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>620768001</pqid></control><display><type>article</type><title>Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Blancher, Antoine ; Tisseyre, Pierre ; Dutaur, Marianne ; Apoil, Pol-André ; Maurer, Claudine ; Quesniaux, Valérie ; Raulf, Friedrich ; Bigaud, Marc ; Abbal, Michel</creator><creatorcontrib>Blancher, Antoine ; Tisseyre, Pierre ; Dutaur, Marianne ; Apoil, Pol-André ; Maurer, Claudine ; Quesniaux, Valérie ; Raulf, Friedrich ; Bigaud, Marc ; Abbal, Michel</creatorcontrib><description>Cynomolgus monkey is one of the macaque species currently used as an animal model for experimental surgery and medicine, in particular, to experiment new drugs or therapy protocols designed for the prevention of allograft rejection. In this field, it is of utmost importance to select histoincompatible recipient-donor pairs. One way to ensure incompatibility between donor and recipient is to check their major histocompatibility complex (MHC) genotypes at the loci playing a determinant role in histocompatibility. We report in this paper on the cynomolgus monkey DRB polymorphism evidenced by sequencing of amplified exon 2 separated either by denaturing gradient gel electrophoresis (DGGE), or by cloning. By the study of 253 unrelated animals from two populations (Mauritius and The Philippines), we characterized 50 exon 2 sequences among which 28 were identical to sequences already reported in Macaca fascicularis or other macaque species (Macaca mulatta, Macaca nemestrina). By cloning and sequencing DRB cDNA, we revealed two additional DRB alleles. Out of the 20 haplotypes that we defined here, only two were found in both populations. The functional impact of DR incompatibility was studied in vitro by mixed lymphocyte culture.</description><identifier>ISSN: 0093-7711</identifier><identifier>EISSN: 1432-1211</identifier><identifier>DOI: 10.1007/s00251-006-0102-9</identifier><identifier>PMID: 16572321</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Amino Acid Sequence ; Animal models ; Animals ; Cells, Cultured ; Cloning ; Cloning, Molecular - methods ; Cynomolgus ; Electrophoresis - methods ; Exons ; Genotype ; Genotypes ; Haplotypes ; HLA-DR Antigens - genetics ; Immunology ; Life Sciences ; Lymphocytes ; Lymphocytes - immunology ; Macaca fascicularis ; Macaca fascicularis - genetics ; Macaca mulatta ; Macaca nemestrina ; Molecular Sequence Data ; Monkeys & apes ; Phylogeny ; Polymerase Chain Reaction - methods ; Polymorphism, Genetic ; Sequence Analysis, DNA - methods ; Sequence Homology, Amino Acid</subject><ispartof>Immunogenetics (New York), 2006-05, Vol.58 (4), p.269-282</ispartof><rights>Springer-Verlag 2006</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-1b60e9650b65e728d903e7b1df58186a449fe63f526bd349dd657f7e2a2297fe3</citedby><cites>FETCH-LOGICAL-c391t-1b60e9650b65e728d903e7b1df58186a449fe63f526bd349dd657f7e2a2297fe3</cites><orcidid>0000-0003-2907-7995 ; 0000-0002-1057-4919</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16572321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00094881$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Blancher, Antoine</creatorcontrib><creatorcontrib>Tisseyre, Pierre</creatorcontrib><creatorcontrib>Dutaur, Marianne</creatorcontrib><creatorcontrib>Apoil, Pol-André</creatorcontrib><creatorcontrib>Maurer, Claudine</creatorcontrib><creatorcontrib>Quesniaux, Valérie</creatorcontrib><creatorcontrib>Raulf, Friedrich</creatorcontrib><creatorcontrib>Bigaud, Marc</creatorcontrib><creatorcontrib>Abbal, Michel</creatorcontrib><title>Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations</title><title>Immunogenetics (New York)</title><addtitle>Immunogenetics</addtitle><description>Cynomolgus monkey is one of the macaque species currently used as an animal model for experimental surgery and medicine, in particular, to experiment new drugs or therapy protocols designed for the prevention of allograft rejection. In this field, it is of utmost importance to select histoincompatible recipient-donor pairs. One way to ensure incompatibility between donor and recipient is to check their major histocompatibility complex (MHC) genotypes at the loci playing a determinant role in histocompatibility. We report in this paper on the cynomolgus monkey DRB polymorphism evidenced by sequencing of amplified exon 2 separated either by denaturing gradient gel electrophoresis (DGGE), or by cloning. By the study of 253 unrelated animals from two populations (Mauritius and The Philippines), we characterized 50 exon 2 sequences among which 28 were identical to sequences already reported in Macaca fascicularis or other macaque species (Macaca mulatta, Macaca nemestrina). By cloning and sequencing DRB cDNA, we revealed two additional DRB alleles. Out of the 20 haplotypes that we defined here, only two were found in both populations. The functional impact of DR incompatibility was studied in vitro by mixed lymphocyte culture.</description><subject>Amino Acid Sequence</subject><subject>Animal models</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cloning</subject><subject>Cloning, Molecular - methods</subject><subject>Cynomolgus</subject><subject>Electrophoresis - methods</subject><subject>Exons</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Haplotypes</subject><subject>HLA-DR Antigens - genetics</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Lymphocytes</subject><subject>Lymphocytes - immunology</subject><subject>Macaca fascicularis</subject><subject>Macaca fascicularis - genetics</subject><subject>Macaca mulatta</subject><subject>Macaca nemestrina</subject><subject>Molecular Sequence Data</subject><subject>Monkeys & apes</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Genetic</subject><subject>Sequence Analysis, DNA - methods</subject><subject>Sequence Homology, Amino Acid</subject><issn>0093-7711</issn><issn>1432-1211</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1v1DAQhi0EokvhB3BBFgfEHlxm7MSOj2X5aKWtKvFxtpzEZlOSOI0Tqvx7vMoKJC7IB1vjZ17NvC8hLxEuEEC9iwA8RwYgGSBwph-RDWaCM-SIj8kGQAumFOIZeRbjHQDmmsun5AxlrrjguCHl12muFxo83S196EL7Y460C_1Pt9C3N7ZKh3obq6aaWzs2cUtvDtWHL--Pn96y9NrSIbRLF8bh0MSONj2dHkKqDalhakIfn5Mn3rbRvTjd5-T7p4_fdldsf_v5ene5Z5XQODEsJTgtcyhl7hQvag3CqRJrnxdYSJtl2jspfM5lWYtM13VawivHLedaeSfOyXbVPdjWDGPT2XExwTbm6nJvjjVIfmRFgb8wsW9WdhjD_eziZLomVq5tbe_CHI1UGvLk139BDlmRXC0S-Pof8C7MY58WNpKDksWqhitUjSHG0fk_cyKYY6RmjTSNKs0xUqNTz6uT8Fx2rv7bccpQ_Aae_5ks</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Blancher, Antoine</creator><creator>Tisseyre, Pierre</creator><creator>Dutaur, Marianne</creator><creator>Apoil, Pol-André</creator><creator>Maurer, Claudine</creator><creator>Quesniaux, Valérie</creator><creator>Raulf, Friedrich</creator><creator>Bigaud, Marc</creator><creator>Abbal, Michel</creator><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2907-7995</orcidid><orcidid>https://orcid.org/0000-0002-1057-4919</orcidid></search><sort><creationdate>20060501</creationdate><title>Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations</title><author>Blancher, Antoine ; Tisseyre, Pierre ; Dutaur, Marianne ; Apoil, Pol-André ; Maurer, Claudine ; Quesniaux, Valérie ; Raulf, Friedrich ; Bigaud, Marc ; Abbal, Michel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-1b60e9650b65e728d903e7b1df58186a449fe63f526bd349dd657f7e2a2297fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Animal models</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cloning</topic><topic>Cloning, Molecular - methods</topic><topic>Cynomolgus</topic><topic>Electrophoresis - methods</topic><topic>Exons</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Haplotypes</topic><topic>HLA-DR Antigens - genetics</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Lymphocytes</topic><topic>Lymphocytes - immunology</topic><topic>Macaca fascicularis</topic><topic>Macaca fascicularis - genetics</topic><topic>Macaca mulatta</topic><topic>Macaca nemestrina</topic><topic>Molecular Sequence Data</topic><topic>Monkeys & apes</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Genetic</topic><topic>Sequence Analysis, DNA - methods</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blancher, Antoine</creatorcontrib><creatorcontrib>Tisseyre, Pierre</creatorcontrib><creatorcontrib>Dutaur, Marianne</creatorcontrib><creatorcontrib>Apoil, Pol-André</creatorcontrib><creatorcontrib>Maurer, Claudine</creatorcontrib><creatorcontrib>Quesniaux, Valérie</creatorcontrib><creatorcontrib>Raulf, Friedrich</creatorcontrib><creatorcontrib>Bigaud, Marc</creatorcontrib><creatorcontrib>Abbal, Michel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Immunogenetics (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blancher, Antoine</au><au>Tisseyre, Pierre</au><au>Dutaur, Marianne</au><au>Apoil, Pol-André</au><au>Maurer, Claudine</au><au>Quesniaux, Valérie</au><au>Raulf, Friedrich</au><au>Bigaud, Marc</au><au>Abbal, Michel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations</atitle><jtitle>Immunogenetics (New York)</jtitle><addtitle>Immunogenetics</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>58</volume><issue>4</issue><spage>269</spage><epage>282</epage><pages>269-282</pages><issn>0093-7711</issn><eissn>1432-1211</eissn><abstract>Cynomolgus monkey is one of the macaque species currently used as an animal model for experimental surgery and medicine, in particular, to experiment new drugs or therapy protocols designed for the prevention of allograft rejection. In this field, it is of utmost importance to select histoincompatible recipient-donor pairs. One way to ensure incompatibility between donor and recipient is to check their major histocompatibility complex (MHC) genotypes at the loci playing a determinant role in histocompatibility. We report in this paper on the cynomolgus monkey DRB polymorphism evidenced by sequencing of amplified exon 2 separated either by denaturing gradient gel electrophoresis (DGGE), or by cloning. By the study of 253 unrelated animals from two populations (Mauritius and The Philippines), we characterized 50 exon 2 sequences among which 28 were identical to sequences already reported in Macaca fascicularis or other macaque species (Macaca mulatta, Macaca nemestrina). By cloning and sequencing DRB cDNA, we revealed two additional DRB alleles. Out of the 20 haplotypes that we defined here, only two were found in both populations. The functional impact of DR incompatibility was studied in vitro by mixed lymphocyte culture.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>16572321</pmid><doi>10.1007/s00251-006-0102-9</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-2907-7995</orcidid><orcidid>https://orcid.org/0000-0002-1057-4919</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0093-7711 |
| ispartof | Immunogenetics (New York), 2006-05, Vol.58 (4), p.269-282 |
| issn | 0093-7711 1432-1211 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00094881v1 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Amino Acid Sequence Animal models Animals Cells, Cultured Cloning Cloning, Molecular - methods Cynomolgus Electrophoresis - methods Exons Genotype Genotypes Haplotypes HLA-DR Antigens - genetics Immunology Life Sciences Lymphocytes Lymphocytes - immunology Macaca fascicularis Macaca fascicularis - genetics Macaca mulatta Macaca nemestrina Molecular Sequence Data Monkeys & apes Phylogeny Polymerase Chain Reaction - methods Polymorphism, Genetic Sequence Analysis, DNA - methods Sequence Homology, Amino Acid |
| title | Study of Cynomolgus monkey (Macaca fascicularis) MhcDRB (Mafa-DRB) polymorphism in two populations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A52%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Study%20of%20Cynomolgus%20monkey%20(Macaca%20fascicularis)%20MhcDRB%20(Mafa-DRB)%20polymorphism%20in%20two%20populations&rft.jtitle=Immunogenetics%20(New%20York)&rft.au=Blancher,%20Antoine&rft.date=2006-05-01&rft.volume=58&rft.issue=4&rft.spage=269&rft.epage=282&rft.pages=269-282&rft.issn=0093-7711&rft.eissn=1432-1211&rft_id=info:doi/10.1007/s00251-006-0102-9&rft_dat=%3Cproquest_hal_p%3E2087443501%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=620768001&rft_id=info:pmid/16572321&rfr_iscdi=true |