New insights into Drosophila larval haemocyte functions through genome‐wide analysis

Summary Drosophila blood cells or haemocytes comprise three cell lineages, plasmatocytes, crystal cells and lamellocytes, involved in immune functions such as phagocytosis, melanisation and encapsulation. Transcriptional profiling of activities of distinct haemocyte populations and from naïve or inf...

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Veröffentlicht in:Cellular microbiology 2005-03, Vol.7 (3), p.335-350
Hauptverfasser: Irving, Phil, Ubeda, Jean‐Michel, Doucet, Daniel, Troxler, Laurent, Lagueux, Marie, Zachary, Daniel, Hoffmann, Jules A., Hetru, Charles, Meister, Marie
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container_end_page 350
container_issue 3
container_start_page 335
container_title Cellular microbiology
container_volume 7
creator Irving, Phil
Ubeda, Jean‐Michel
Doucet, Daniel
Troxler, Laurent
Lagueux, Marie
Zachary, Daniel
Hoffmann, Jules A.
Hetru, Charles
Meister, Marie
description Summary Drosophila blood cells or haemocytes comprise three cell lineages, plasmatocytes, crystal cells and lamellocytes, involved in immune functions such as phagocytosis, melanisation and encapsulation. Transcriptional profiling of activities of distinct haemocyte populations and from naïve or infected larvae, was performed to find genes contributing to haemocyte functions. Of the 13 000 genes represented on the microarray, over 2500 exhibited significantly enriched transcription in haemocytes. Among these were genes encoding integrins, peptidoglycan recognition proteins (PGRPs), scavenger receptors, lectins, cell adhesion molecules and serine proteases. One relevant outcome of this analysis was the gain of new insights into the lamellocyte encapsulation process. We showed that lamellocytes require βPS integrin for encapsulation and that they transcribe one prophenoloxidase gene enabling them to produce the enzyme necessary for melanisation of the capsule. A second compelling observation was that following infection, the gene encoding the cytokine Spätzle was uniquely upregulated in haemocytes and not the fat body. This shows that Drosophila haemocytes produce a signal molecule ready to be activated through cleavage after pathogen recognition, informing distant tissues of infection.
doi_str_mv 10.1111/j.1462-5822.2004.00462.x
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Transcriptional profiling of activities of distinct haemocyte populations and from naïve or infected larvae, was performed to find genes contributing to haemocyte functions. Of the 13 000 genes represented on the microarray, over 2500 exhibited significantly enriched transcription in haemocytes. Among these were genes encoding integrins, peptidoglycan recognition proteins (PGRPs), scavenger receptors, lectins, cell adhesion molecules and serine proteases. One relevant outcome of this analysis was the gain of new insights into the lamellocyte encapsulation process. We showed that lamellocytes require βPS integrin for encapsulation and that they transcribe one prophenoloxidase gene enabling them to produce the enzyme necessary for melanisation of the capsule. A second compelling observation was that following infection, the gene encoding the cytokine Spätzle was uniquely upregulated in haemocytes and not the fat body. 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Ubeda, Jean‐Michel ; Doucet, Daniel ; Troxler, Laurent ; Lagueux, Marie ; Zachary, Daniel ; Hoffmann, Jules A. ; Hetru, Charles ; Meister, Marie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4002-c6ff324218f2cd9cf7b9dd0ef3cca35154508d813cde9ab028465a05f7ece4663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biochemistry, Molecular Biology</topic><topic>Catechol Oxidase - genetics</topic><topic>Catechol Oxidase - metabolism</topic><topic>Cell Lineage</topic><topic>Cellular Biology</topic><topic>Drosophila - genetics</topic><topic>Drosophila - immunology</topic><topic>Drosophila - microbiology</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Enzyme Precursors - genetics</topic><topic>Enzyme Precursors - metabolism</topic><topic>Escherichia coli - pathogenicity</topic><topic>Fat Body - metabolism</topic><topic>Fat Body - microbiology</topic><topic>Gene Expression Profiling</topic><topic>Genome</topic><topic>Hemocytes - immunology</topic><topic>Hemocytes - metabolism</topic><topic>Hemocytes - microbiology</topic><topic>Integrin alpha Chains</topic><topic>Integrins - genetics</topic><topic>Integrins - metabolism</topic><topic>Larva - genetics</topic><topic>Larva - immunology</topic><topic>Larva - microbiology</topic><topic>Life Sciences</topic><topic>Micrococcus luteus - pathogenicity</topic><topic>Molecular biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Irving, Phil</creatorcontrib><creatorcontrib>Ubeda, Jean‐Michel</creatorcontrib><creatorcontrib>Doucet, Daniel</creatorcontrib><creatorcontrib>Troxler, Laurent</creatorcontrib><creatorcontrib>Lagueux, Marie</creatorcontrib><creatorcontrib>Zachary, Daniel</creatorcontrib><creatorcontrib>Hoffmann, Jules A.</creatorcontrib><creatorcontrib>Hetru, Charles</creatorcontrib><creatorcontrib>Meister, Marie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Cellular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Irving, Phil</au><au>Ubeda, Jean‐Michel</au><au>Doucet, Daniel</au><au>Troxler, Laurent</au><au>Lagueux, Marie</au><au>Zachary, Daniel</au><au>Hoffmann, Jules A.</au><au>Hetru, Charles</au><au>Meister, Marie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New insights into Drosophila larval haemocyte functions through genome‐wide analysis</atitle><jtitle>Cellular microbiology</jtitle><addtitle>Cell Microbiol</addtitle><date>2005-03</date><risdate>2005</risdate><volume>7</volume><issue>3</issue><spage>335</spage><epage>350</epage><pages>335-350</pages><issn>1462-5814</issn><eissn>1462-5822</eissn><abstract>Summary Drosophila blood cells or haemocytes comprise three cell lineages, plasmatocytes, crystal cells and lamellocytes, involved in immune functions such as phagocytosis, melanisation and encapsulation. Transcriptional profiling of activities of distinct haemocyte populations and from naïve or infected larvae, was performed to find genes contributing to haemocyte functions. Of the 13 000 genes represented on the microarray, over 2500 exhibited significantly enriched transcription in haemocytes. Among these were genes encoding integrins, peptidoglycan recognition proteins (PGRPs), scavenger receptors, lectins, cell adhesion molecules and serine proteases. One relevant outcome of this analysis was the gain of new insights into the lamellocyte encapsulation process. We showed that lamellocytes require βPS integrin for encapsulation and that they transcribe one prophenoloxidase gene enabling them to produce the enzyme necessary for melanisation of the capsule. A second compelling observation was that following infection, the gene encoding the cytokine Spätzle was uniquely upregulated in haemocytes and not the fat body. This shows that Drosophila haemocytes produce a signal molecule ready to be activated through cleavage after pathogen recognition, informing distant tissues of infection.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15679837</pmid><doi>10.1111/j.1462-5822.2004.00462.x</doi><tpages>16</tpages></addata></record>
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subjects Animals
Biochemistry, Molecular Biology
Catechol Oxidase - genetics
Catechol Oxidase - metabolism
Cell Lineage
Cellular Biology
Drosophila - genetics
Drosophila - immunology
Drosophila - microbiology
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Enzyme Precursors - genetics
Enzyme Precursors - metabolism
Escherichia coli - pathogenicity
Fat Body - metabolism
Fat Body - microbiology
Gene Expression Profiling
Genome
Hemocytes - immunology
Hemocytes - metabolism
Hemocytes - microbiology
Integrin alpha Chains
Integrins - genetics
Integrins - metabolism
Larva - genetics
Larva - immunology
Larva - microbiology
Life Sciences
Micrococcus luteus - pathogenicity
Molecular biology
title New insights into Drosophila larval haemocyte functions through genome‐wide analysis
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