Role of Importin-β in Coupling Ran to Downstream Targets in Microtubule Assembly
The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclearmitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce ast...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2001-01, Vol.291 (5504), p.653-656 |
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| creator | Wiese, Christiane Wilde, Andrew Moore, Mary Shannon Adam, Stephen A. Merdes, Andreas Zheng, Yixian |
| description | The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclearmitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-β. We show that importin-β is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-β and NuMA. Importin-β therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis. |
| doi_str_mv | 10.1126/science.1057661 |
| format | Article |
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A carboxyl-terminal region of the nuclearmitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-β. We show that importin-β is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-β and NuMA. Importin-β therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1057661</identifier><identifier>PMID: 11229403</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Amino acids ; Animals ; Antibodies ; Biological and medical sciences ; Cell cycle ; Cell cycle, cell proliferation ; Cell Extracts ; Cell Nucleus - metabolism ; Cell physiology ; Cellular Biology ; Chromatin ; Eggs ; Embryos ; Fundamental and applied biological sciences. Psychology ; Gene expression regulation ; Guanine triphosphatase ; Guanosine Triphosphate - metabolism ; importin-^b ; Interphase ; Karyopherins ; Life Sciences ; Microtubules ; Microtubules - drug effects ; Microtubules - metabolism ; Mitosis ; Mitotic spindle apparatus ; Models, Biological ; Molecular and cellular biology ; nuclear import ; Nuclear Proteins - metabolism ; Nuclear Proteins - pharmacology ; NuMA protein ; Ova ; Ovum ; Paclitaxel - pharmacology ; Proteins ; ran GTP-Binding Protein - metabolism ; Ran protein ; Recombinant Fusion Proteins - metabolism ; Reptiles & amphibians ; Spindle Apparatus - drug effects ; Spindle Apparatus - metabolism ; Xenopus ; Xenopus Proteins</subject><ispartof>Science (American Association for the Advancement of Science), 2001-01, Vol.291 (5504), p.653-656</ispartof><rights>Copyright 2001 American Association for the Advancement of Science</rights><rights>2001 INIST-CNRS</rights><rights>COPYRIGHT 2001 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Jan 26, 2001</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c744t-be51ff03ba36ed33d250305564c543a1617dc90ab8cffc885e981969d94aafef3</citedby><cites>FETCH-LOGICAL-c744t-be51ff03ba36ed33d250305564c543a1617dc90ab8cffc885e981969d94aafef3</cites><orcidid>0000-0002-3739-2728 ; 0000-0001-8964-5603</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3082239$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3082239$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=928783$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11229403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00091100$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Wiese, Christiane</creatorcontrib><creatorcontrib>Wilde, Andrew</creatorcontrib><creatorcontrib>Moore, Mary Shannon</creatorcontrib><creatorcontrib>Adam, Stephen A.</creatorcontrib><creatorcontrib>Merdes, Andreas</creatorcontrib><creatorcontrib>Zheng, Yixian</creatorcontrib><title>Role of Importin-β in Coupling Ran to Downstream Targets in Microtubule Assembly</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclearmitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-β. We show that importin-β is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-β and NuMA. Importin-β therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Cell cycle</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell Extracts</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell physiology</subject><subject>Cellular Biology</subject><subject>Chromatin</subject><subject>Eggs</subject><subject>Embryos</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression regulation</subject><subject>Guanine triphosphatase</subject><subject>Guanosine Triphosphate - metabolism</subject><subject>importin-^b</subject><subject>Interphase</subject><subject>Karyopherins</subject><subject>Life Sciences</subject><subject>Microtubules</subject><subject>Microtubules - drug effects</subject><subject>Microtubules - metabolism</subject><subject>Mitosis</subject><subject>Mitotic spindle apparatus</subject><subject>Models, Biological</subject><subject>Molecular and cellular biology</subject><subject>nuclear import</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nuclear Proteins - pharmacology</subject><subject>NuMA protein</subject><subject>Ova</subject><subject>Ovum</subject><subject>Paclitaxel - pharmacology</subject><subject>Proteins</subject><subject>ran GTP-Binding Protein - metabolism</subject><subject>Ran protein</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Reptiles & amphibians</subject><subject>Spindle Apparatus - 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| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2001-01, Vol.291 (5504), p.653-656 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00091100v1 |
| source | MEDLINE; American Association for the Advancement of Science; Jstor Complete Legacy |
| subjects | Amino acids Animals Antibodies Biological and medical sciences Cell cycle Cell cycle, cell proliferation Cell Extracts Cell Nucleus - metabolism Cell physiology Cellular Biology Chromatin Eggs Embryos Fundamental and applied biological sciences. Psychology Gene expression regulation Guanine triphosphatase Guanosine Triphosphate - metabolism importin-^b Interphase Karyopherins Life Sciences Microtubules Microtubules - drug effects Microtubules - metabolism Mitosis Mitotic spindle apparatus Models, Biological Molecular and cellular biology nuclear import Nuclear Proteins - metabolism Nuclear Proteins - pharmacology NuMA protein Ova Ovum Paclitaxel - pharmacology Proteins ran GTP-Binding Protein - metabolism Ran protein Recombinant Fusion Proteins - metabolism Reptiles & amphibians Spindle Apparatus - drug effects Spindle Apparatus - metabolism Xenopus Xenopus Proteins |
| title | Role of Importin-β in Coupling Ran to Downstream Targets in Microtubule Assembly |
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