AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis

The sucrose transporter gene AtSUC5 was studied as part of a programme aimed at identifying and studying the genes involved in seed maturation in Arabidopsis. Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed...

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Veröffentlicht in:The Plant journal : for cell and molecular biology 2005-09, Vol.43 (6), p.824-836
Hauptverfasser: Baud, S, Wuilleme, S, Lemoine, R, Kronenberger, J, Caboche, M, Lepiniec, L, Rochart, C
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container_title The Plant journal : for cell and molecular biology
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creator Baud, S
Wuilleme, S
Lemoine, R
Kronenberger, J
Caboche, M
Lepiniec, L
Rochart, C
description The sucrose transporter gene AtSUC5 was studied as part of a programme aimed at identifying and studying the genes involved in seed maturation in Arabidopsis. Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed that the gene was specifically and highly induced during seed development between 4 and 9 days after flowering (DAF). Analysis of the activity of the AtSUC5 promoter in planta was consistent with this timing, and suggested that AtSUC5 expression is endosperm specific, spreading from the micropylar to the chalazal pole of the filial tissue. To demonstrate the function of AtSUC5, the corresponding cDNA was used to complement a sucrose uptake-deficient yeast mutant, thus confirming its sucrose transport capacity. To investigate the function in planta, three allelic mutants disrupted in the AtSUC5 gene were isolated and characterized. A strong but transient reduction in fatty acid concentration was observed in mutant seeds 8 DAF. This biochemical phenotype was associated with a slight delay in embryo development. Taken together, these data demonstrated the role of the AtSUC5 carrier in the nutrition of the filial tissues during early seed development. However, additional sugar uptake systems, which remain to be characterized, must be functional in developing seeds, especially during maturation of the embryo.
doi_str_mv 10.1111/j.1365-313X.2005.02496.x
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Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed that the gene was specifically and highly induced during seed development between 4 and 9 days after flowering (DAF). Analysis of the activity of the AtSUC5 promoter in planta was consistent with this timing, and suggested that AtSUC5 expression is endosperm specific, spreading from the micropylar to the chalazal pole of the filial tissue. To demonstrate the function of AtSUC5, the corresponding cDNA was used to complement a sucrose uptake-deficient yeast mutant, thus confirming its sucrose transport capacity. To investigate the function in planta, three allelic mutants disrupted in the AtSUC5 gene were isolated and characterized. A strong but transient reduction in fatty acid concentration was observed in mutant seeds 8 DAF. This biochemical phenotype was associated with a slight delay in embryo development. 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Psychology ; gene expression regulation ; Gene Expression Regulation, Developmental - physiology ; Gene Expression Regulation, Plant - physiology ; Genes ; in situ hybridization ; Life Sciences ; Membrane Transport Proteins - biosynthesis ; Membrane Transport Proteins - physiology ; Multigene Family ; mutants ; nucleotide sequences ; plant biochemistry ; plant genetics ; plant physiology ; Plant physiology and development ; plant proteins ; Plant Proteins - biosynthesis ; Plant Proteins - physiology ; Plant reproduction ; promoter regions ; Promoter Regions, Genetic - physiology ; recombinant fusion proteins ; reverse transcriptase polymerase chain reaction ; seed ; seed development ; Seeds ; Seeds - growth &amp; development ; Seeds - metabolism ; sucrose ; sucrose transporter ; Sugar ; transporters ; Vegetal Biology ; Vegetative and sexual reproduction, floral biology, fructification</subject><ispartof>The Plant journal : for cell and molecular biology, 2005-09, Vol.43 (6), p.824-836</ispartof><rights>2005 INIST-CNRS</rights><rights>2005 Blackwell Publishing Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5686-3f9bcbc8ec9b54e46c0b6c75fa04d411d6addeeb9f02af6a32376bb042032b443</citedby><cites>FETCH-LOGICAL-c5686-3f9bcbc8ec9b54e46c0b6c75fa04d411d6addeeb9f02af6a32376bb042032b443</cites><orcidid>0000-0002-5507-327X ; 0000-0002-5845-3323</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2005.02496.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2005.02496.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,777,781,882,1412,1428,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=17073509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16146522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00078925$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Baud, S</creatorcontrib><creatorcontrib>Wuilleme, S</creatorcontrib><creatorcontrib>Lemoine, R</creatorcontrib><creatorcontrib>Kronenberger, J</creatorcontrib><creatorcontrib>Caboche, M</creatorcontrib><creatorcontrib>Lepiniec, L</creatorcontrib><creatorcontrib>Rochart, C</creatorcontrib><title>AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>The sucrose transporter gene AtSUC5 was studied as part of a programme aimed at identifying and studying the genes involved in seed maturation in Arabidopsis. Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed that the gene was specifically and highly induced during seed development between 4 and 9 days after flowering (DAF). Analysis of the activity of the AtSUC5 promoter in planta was consistent with this timing, and suggested that AtSUC5 expression is endosperm specific, spreading from the micropylar to the chalazal pole of the filial tissue. To demonstrate the function of AtSUC5, the corresponding cDNA was used to complement a sucrose uptake-deficient yeast mutant, thus confirming its sucrose transport capacity. To investigate the function in planta, three allelic mutants disrupted in the AtSUC5 gene were isolated and characterized. A strong but transient reduction in fatty acid concentration was observed in mutant seeds 8 DAF. This biochemical phenotype was associated with a slight delay in embryo development. 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subjects Arabidopsis
Arabidopsis - embryology
Arabidopsis - metabolism
Arabidopsis thaliana
Biological and medical sciences
embryogenesis
endosperm
fatty acid composition
fatty acids
Fructification and ripening
Fundamental and applied biological sciences. Psychology
gene expression regulation
Gene Expression Regulation, Developmental - physiology
Gene Expression Regulation, Plant - physiology
Genes
in situ hybridization
Life Sciences
Membrane Transport Proteins - biosynthesis
Membrane Transport Proteins - physiology
Multigene Family
mutants
nucleotide sequences
plant biochemistry
plant genetics
plant physiology
Plant physiology and development
plant proteins
Plant Proteins - biosynthesis
Plant Proteins - physiology
Plant reproduction
promoter regions
Promoter Regions, Genetic - physiology
recombinant fusion proteins
reverse transcriptase polymerase chain reaction
seed
seed development
Seeds
Seeds - growth & development
Seeds - metabolism
sucrose
sucrose transporter
Sugar
transporters
Vegetal Biology
Vegetative and sexual reproduction, floral biology, fructification
title AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis
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