AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis
The sucrose transporter gene AtSUC5 was studied as part of a programme aimed at identifying and studying the genes involved in seed maturation in Arabidopsis. Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed...
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description | The sucrose transporter gene AtSUC5 was studied as part of a programme aimed at identifying and studying the genes involved in seed maturation in Arabidopsis. Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed that the gene was specifically and highly induced during seed development between 4 and 9 days after flowering (DAF). Analysis of the activity of the AtSUC5 promoter in planta was consistent with this timing, and suggested that AtSUC5 expression is endosperm specific, spreading from the micropylar to the chalazal pole of the filial tissue. To demonstrate the function of AtSUC5, the corresponding cDNA was used to complement a sucrose uptake-deficient yeast mutant, thus confirming its sucrose transport capacity. To investigate the function in planta, three allelic mutants disrupted in the AtSUC5 gene were isolated and characterized. A strong but transient reduction in fatty acid concentration was observed in mutant seeds 8 DAF. This biochemical phenotype was associated with a slight delay in embryo development. Taken together, these data demonstrated the role of the AtSUC5 carrier in the nutrition of the filial tissues during early seed development. However, additional sugar uptake systems, which remain to be characterized, must be functional in developing seeds, especially during maturation of the embryo. |
doi_str_mv | 10.1111/j.1365-313X.2005.02496.x |
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Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed that the gene was specifically and highly induced during seed development between 4 and 9 days after flowering (DAF). Analysis of the activity of the AtSUC5 promoter in planta was consistent with this timing, and suggested that AtSUC5 expression is endosperm specific, spreading from the micropylar to the chalazal pole of the filial tissue. To demonstrate the function of AtSUC5, the corresponding cDNA was used to complement a sucrose uptake-deficient yeast mutant, thus confirming its sucrose transport capacity. To investigate the function in planta, three allelic mutants disrupted in the AtSUC5 gene were isolated and characterized. A strong but transient reduction in fatty acid concentration was observed in mutant seeds 8 DAF. This biochemical phenotype was associated with a slight delay in embryo development. Taken together, these data demonstrated the role of the AtSUC5 carrier in the nutrition of the filial tissues during early seed development. However, additional sugar uptake systems, which remain to be characterized, must be functional in developing seeds, especially during maturation of the embryo.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313X.2005.02496.x</identifier><identifier>PMID: 16146522</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Arabidopsis ; Arabidopsis - embryology ; Arabidopsis - metabolism ; Arabidopsis thaliana ; Biological and medical sciences ; embryogenesis ; endosperm ; fatty acid composition ; fatty acids ; Fructification and ripening ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; Gene Expression Regulation, Developmental - physiology ; Gene Expression Regulation, Plant - physiology ; Genes ; in situ hybridization ; Life Sciences ; Membrane Transport Proteins - biosynthesis ; Membrane Transport Proteins - physiology ; Multigene Family ; mutants ; nucleotide sequences ; plant biochemistry ; plant genetics ; plant physiology ; Plant physiology and development ; plant proteins ; Plant Proteins - biosynthesis ; Plant Proteins - physiology ; Plant reproduction ; promoter regions ; Promoter Regions, Genetic - physiology ; recombinant fusion proteins ; reverse transcriptase polymerase chain reaction ; seed ; seed development ; Seeds ; Seeds - growth & development ; Seeds - metabolism ; sucrose ; sucrose transporter ; Sugar ; transporters ; Vegetal Biology ; Vegetative and sexual reproduction, floral biology, fructification</subject><ispartof>The Plant journal : for cell and molecular biology, 2005-09, Vol.43 (6), p.824-836</ispartof><rights>2005 INIST-CNRS</rights><rights>2005 Blackwell Publishing Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5686-3f9bcbc8ec9b54e46c0b6c75fa04d411d6addeeb9f02af6a32376bb042032b443</citedby><cites>FETCH-LOGICAL-c5686-3f9bcbc8ec9b54e46c0b6c75fa04d411d6addeeb9f02af6a32376bb042032b443</cites><orcidid>0000-0002-5507-327X ; 0000-0002-5845-3323</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2005.02496.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2005.02496.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,777,781,882,1412,1428,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17073509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16146522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00078925$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Baud, S</creatorcontrib><creatorcontrib>Wuilleme, S</creatorcontrib><creatorcontrib>Lemoine, R</creatorcontrib><creatorcontrib>Kronenberger, J</creatorcontrib><creatorcontrib>Caboche, M</creatorcontrib><creatorcontrib>Lepiniec, L</creatorcontrib><creatorcontrib>Rochart, C</creatorcontrib><title>AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>The sucrose transporter gene AtSUC5 was studied as part of a programme aimed at identifying and studying the genes involved in seed maturation in Arabidopsis. Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed that the gene was specifically and highly induced during seed development between 4 and 9 days after flowering (DAF). Analysis of the activity of the AtSUC5 promoter in planta was consistent with this timing, and suggested that AtSUC5 expression is endosperm specific, spreading from the micropylar to the chalazal pole of the filial tissue. To demonstrate the function of AtSUC5, the corresponding cDNA was used to complement a sucrose uptake-deficient yeast mutant, thus confirming its sucrose transport capacity. To investigate the function in planta, three allelic mutants disrupted in the AtSUC5 gene were isolated and characterized. A strong but transient reduction in fatty acid concentration was observed in mutant seeds 8 DAF. This biochemical phenotype was associated with a slight delay in embryo development. Taken together, these data demonstrated the role of the AtSUC5 carrier in the nutrition of the filial tissues during early seed development. However, additional sugar uptake systems, which remain to be characterized, must be functional in developing seeds, especially during maturation of the embryo.</description><subject>Arabidopsis</subject><subject>Arabidopsis - embryology</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>embryogenesis</subject><subject>endosperm</subject><subject>fatty acid composition</subject><subject>fatty acids</subject><subject>Fructification and ripening</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Genes</subject><subject>in situ hybridization</subject><subject>Life Sciences</subject><subject>Membrane Transport Proteins - biosynthesis</subject><subject>Membrane Transport Proteins - physiology</subject><subject>Multigene Family</subject><subject>mutants</subject><subject>nucleotide sequences</subject><subject>plant biochemistry</subject><subject>plant genetics</subject><subject>plant physiology</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>Plant Proteins - biosynthesis</subject><subject>Plant Proteins - physiology</subject><subject>Plant reproduction</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>recombinant fusion proteins</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>seed</subject><subject>seed development</subject><subject>Seeds</subject><subject>Seeds - growth & development</subject><subject>Seeds - metabolism</subject><subject>sucrose</subject><subject>sucrose transporter</subject><subject>Sugar</subject><subject>transporters</subject><subject>Vegetal Biology</subject><subject>Vegetative and sexual reproduction, floral biology, fructification</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd9v0zAQgCMEYt3gX4AICSQeUs4_kzzwUFXAQJVA2irxZjnOhblK48xOu_a_n7NUm8QL-MX23Xfns74kSQnMSVyfNnPCpMgYYb_nFEDMgfJSzg_Pktlj4nkyg1JClnNCz5LzEDYAJGeSv0zOiCRcCkpnyd1iuFovRRp2xruA6eB1F3rnB_Rp6NHYxhrdtscUD73HELBObZcON5hiV7tI-G1qQ4ztXbufkqh95APGW417bF2_xW4YMwuvK1u7PtjwKnnR6Dbg69N-kay_frleXmarn9--LxerzAhZyIw1ZWUqU6ApK8GRSwOVNLloNPCaE1JLXdeIVdkA1Y3UjLJcVhVwCoxWnLOL5OPU90a3qvd2q_1ROW3V5WKlxhgA5EVJxZ5E9sPE9t7d7jAMamuDwbbVHbpdULIQkhcM_gmSXBSiFGUE3_0FbtzOd_HDihIWlcmHEYsJGg0Ej83jnATUqFtt1GhVjVbVqFs96FaHWPrm1H9XbbF-Kjz5jcD7E6BD9NhEu8aGJy6HnAkYB_08cXe2xeN_D6Cuf_0YT7H-7VTfaKf0Hx_fWF9RIAwIMFLEX94DgHDP7w</recordid><startdate>200509</startdate><enddate>200509</enddate><creator>Baud, S</creator><creator>Wuilleme, S</creator><creator>Lemoine, R</creator><creator>Kronenberger, J</creator><creator>Caboche, M</creator><creator>Lepiniec, L</creator><creator>Rochart, C</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5507-327X</orcidid><orcidid>https://orcid.org/0000-0002-5845-3323</orcidid></search><sort><creationdate>200509</creationdate><title>AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis</title><author>Baud, S ; Wuilleme, S ; Lemoine, R ; Kronenberger, J ; Caboche, M ; Lepiniec, L ; Rochart, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5686-3f9bcbc8ec9b54e46c0b6c75fa04d411d6addeeb9f02af6a32376bb042032b443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis - embryology</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>embryogenesis</topic><topic>endosperm</topic><topic>fatty acid composition</topic><topic>fatty acids</topic><topic>Fructification and ripening</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Genes</topic><topic>in situ hybridization</topic><topic>Life Sciences</topic><topic>Membrane Transport Proteins - biosynthesis</topic><topic>Membrane Transport Proteins - physiology</topic><topic>Multigene Family</topic><topic>mutants</topic><topic>nucleotide sequences</topic><topic>plant biochemistry</topic><topic>plant genetics</topic><topic>plant physiology</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>Plant Proteins - biosynthesis</topic><topic>Plant Proteins - physiology</topic><topic>Plant reproduction</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>recombinant fusion proteins</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>seed</topic><topic>seed development</topic><topic>Seeds</topic><topic>Seeds - growth & development</topic><topic>Seeds - metabolism</topic><topic>sucrose</topic><topic>sucrose transporter</topic><topic>Sugar</topic><topic>transporters</topic><topic>Vegetal Biology</topic><topic>Vegetative and sexual reproduction, floral biology, fructification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baud, S</creatorcontrib><creatorcontrib>Wuilleme, S</creatorcontrib><creatorcontrib>Lemoine, R</creatorcontrib><creatorcontrib>Kronenberger, J</creatorcontrib><creatorcontrib>Caboche, M</creatorcontrib><creatorcontrib>Lepiniec, L</creatorcontrib><creatorcontrib>Rochart, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baud, S</au><au>Wuilleme, S</au><au>Lemoine, R</au><au>Kronenberger, J</au><au>Caboche, M</au><au>Lepiniec, L</au><au>Rochart, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2005-09</date><risdate>2005</risdate><volume>43</volume><issue>6</issue><spage>824</spage><epage>836</epage><pages>824-836</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>The sucrose transporter gene AtSUC5 was studied as part of a programme aimed at identifying and studying the genes involved in seed maturation in Arabidopsis. Expression profiling of AtSUC5 using the technique of real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) showed that the gene was specifically and highly induced during seed development between 4 and 9 days after flowering (DAF). Analysis of the activity of the AtSUC5 promoter in planta was consistent with this timing, and suggested that AtSUC5 expression is endosperm specific, spreading from the micropylar to the chalazal pole of the filial tissue. To demonstrate the function of AtSUC5, the corresponding cDNA was used to complement a sucrose uptake-deficient yeast mutant, thus confirming its sucrose transport capacity. To investigate the function in planta, three allelic mutants disrupted in the AtSUC5 gene were isolated and characterized. A strong but transient reduction in fatty acid concentration was observed in mutant seeds 8 DAF. This biochemical phenotype was associated with a slight delay in embryo development. Taken together, these data demonstrated the role of the AtSUC5 carrier in the nutrition of the filial tissues during early seed development. However, additional sugar uptake systems, which remain to be characterized, must be functional in developing seeds, especially during maturation of the embryo.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16146522</pmid><doi>10.1111/j.1365-313X.2005.02496.x</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-5507-327X</orcidid><orcidid>https://orcid.org/0000-0002-5845-3323</orcidid></addata></record> |
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subjects | Arabidopsis Arabidopsis - embryology Arabidopsis - metabolism Arabidopsis thaliana Biological and medical sciences embryogenesis endosperm fatty acid composition fatty acids Fructification and ripening Fundamental and applied biological sciences. Psychology gene expression regulation Gene Expression Regulation, Developmental - physiology Gene Expression Regulation, Plant - physiology Genes in situ hybridization Life Sciences Membrane Transport Proteins - biosynthesis Membrane Transport Proteins - physiology Multigene Family mutants nucleotide sequences plant biochemistry plant genetics plant physiology Plant physiology and development plant proteins Plant Proteins - biosynthesis Plant Proteins - physiology Plant reproduction promoter regions Promoter Regions, Genetic - physiology recombinant fusion proteins reverse transcriptase polymerase chain reaction seed seed development Seeds Seeds - growth & development Seeds - metabolism sucrose sucrose transporter Sugar transporters Vegetal Biology Vegetative and sexual reproduction, floral biology, fructification |
title | AtSUC5 sucrose transporter specifically expressed in the endosperm is involved in early seed development in Arabidopsis |
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