The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria
Summary The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and – molecular‐based analysis. Thirty‐one isolates, representative of each dominant morphotypes, were affiliated to A...
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| Veröffentlicht in: | Environmental microbiology 2006-03, Vol.8 (3), p.514-525 |
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| creator | Chanal, Angélique Chapon, Virginie Benzerara, Karim Barakat, Mohamed Christen, Richard Achouak, Wafa Barras, Frédéric Heulin, Thierry |
| description | Summary
The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and – molecular‐based analysis. Thirty‐one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria, Firmicutes, Proteobacteria and the CFB group while none related to Archaea. Analysis of 16S rRNA gene libraries revealed the presence of species related to Bacteria and Archaea. Sequences related to Archaea were all affiliated to the non‐thermophilic Crenarchaeota subgroup. Bacterial sequences were dominated by Proteobacteria, Actinobacteria and Acidobacteria; a few sequences were distributed among eight others phyla, including Thermus/Deinococcus relatives. A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans. Because bacteria living in the hot desert of Tataouine are one way or another tolerant to desiccation, we investigate whether they could also be tolerant to radiation. Exposition of soil samples to intense gamma radiation yields Bacillus, Thermus/Deinococcus and α‐Proteobacteria relatives. Four of these strains correspond to radiotolerant species as revealed by evaluation of the resistance levels of the individual cultures. A detailed analysis of the resistance levels for two Thermus/Deinococcus and two α‐Proteobacteria relatives revealed that they correspond to new radiotolerant species. |
| doi_str_mv | 10.1111/j.1462-2920.2005.00921.x |
| format | Article |
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The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and – molecular‐based analysis. Thirty‐one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria, Firmicutes, Proteobacteria and the CFB group while none related to Archaea. Analysis of 16S rRNA gene libraries revealed the presence of species related to Bacteria and Archaea. Sequences related to Archaea were all affiliated to the non‐thermophilic Crenarchaeota subgroup. Bacterial sequences were dominated by Proteobacteria, Actinobacteria and Acidobacteria; a few sequences were distributed among eight others phyla, including Thermus/Deinococcus relatives. A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans. Because bacteria living in the hot desert of Tataouine are one way or another tolerant to desiccation, we investigate whether they could also be tolerant to radiation. Exposition of soil samples to intense gamma radiation yields Bacillus, Thermus/Deinococcus and α‐Proteobacteria relatives. Four of these strains correspond to radiotolerant species as revealed by evaluation of the resistance levels of the individual cultures. A detailed analysis of the resistance levels for two Thermus/Deinococcus and two α‐Proteobacteria relatives revealed that they correspond to new radiotolerant species.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/j.1462-2920.2005.00921.x</identifier><identifier>PMID: 16478457</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Actinobacteria ; Alphaproteobacteria ; Archaea ; Archaea - classification ; Archaea - genetics ; Archaea - isolation & purification ; Bacillus ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteria - radiation effects ; Biodiversity ; Crenarchaeota ; Deinococcus radiodurans ; Desert Climate ; Desiccation ; DNA, Archaeal - chemistry ; DNA, Archaeal - genetics ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Ecosystem ; Firmicutes ; Molecular Sequence Data ; Phylogeny ; Proteobacteria ; Radiation, Ionizing ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Soil Microbiology ; Tunisia</subject><ispartof>Environmental microbiology, 2006-03, Vol.8 (3), p.514-525</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4691-56b23f6912385dacac1f48403e6b8dbf5eb1bf238fd7995af9b8716e2c3489653</citedby><cites>FETCH-LOGICAL-c4691-56b23f6912385dacac1f48403e6b8dbf5eb1bf238fd7995af9b8716e2c3489653</cites><orcidid>0000-0002-0553-0137 ; 0000-0003-4297-6661 ; 0000-0002-2365-3464 ; 0000-0003-3458-2574</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1462-2920.2005.00921.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1462-2920.2005.00921.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16478457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00022579$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Chanal, Angélique</creatorcontrib><creatorcontrib>Chapon, Virginie</creatorcontrib><creatorcontrib>Benzerara, Karim</creatorcontrib><creatorcontrib>Barakat, Mohamed</creatorcontrib><creatorcontrib>Christen, Richard</creatorcontrib><creatorcontrib>Achouak, Wafa</creatorcontrib><creatorcontrib>Barras, Frédéric</creatorcontrib><creatorcontrib>Heulin, Thierry</creatorcontrib><title>The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria</title><title>Environmental microbiology</title><addtitle>Environ Microbiol</addtitle><description>Summary
The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and – molecular‐based analysis. Thirty‐one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria, Firmicutes, Proteobacteria and the CFB group while none related to Archaea. Analysis of 16S rRNA gene libraries revealed the presence of species related to Bacteria and Archaea. Sequences related to Archaea were all affiliated to the non‐thermophilic Crenarchaeota subgroup. Bacterial sequences were dominated by Proteobacteria, Actinobacteria and Acidobacteria; a few sequences were distributed among eight others phyla, including Thermus/Deinococcus relatives. A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans. Because bacteria living in the hot desert of Tataouine are one way or another tolerant to desiccation, we investigate whether they could also be tolerant to radiation. Exposition of soil samples to intense gamma radiation yields Bacillus, Thermus/Deinococcus and α‐Proteobacteria relatives. Four of these strains correspond to radiotolerant species as revealed by evaluation of the resistance levels of the individual cultures. A detailed analysis of the resistance levels for two Thermus/Deinococcus and two α‐Proteobacteria relatives revealed that they correspond to new radiotolerant species.</description><subject>Actinobacteria</subject><subject>Alphaproteobacteria</subject><subject>Archaea</subject><subject>Archaea - classification</subject><subject>Archaea - genetics</subject><subject>Archaea - isolation & purification</subject><subject>Bacillus</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - radiation effects</subject><subject>Biodiversity</subject><subject>Crenarchaeota</subject><subject>Deinococcus radiodurans</subject><subject>Desert Climate</subject><subject>Desiccation</subject><subject>DNA, Archaeal - chemistry</subject><subject>DNA, Archaeal - genetics</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ecosystem</subject><subject>Firmicutes</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Proteobacteria</subject><subject>Radiation, Ionizing</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Soil Microbiology</subject><subject>Tunisia</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhSMEoqXwF5BPSByy2E5ix4hLVZVupaUgsagSF2uSTFgvSVxs7zb773HIajmCLx55vvfG9ksSwuiCxfVuu2C54ClXnC44pcWCUsXZYnySnJ8aT08142fJC--3lDKZSfo8OWMil2VeyPNkXG-QNOjRBWJbsoYAdmcGfE9gIDgGhz0SHPbG2aHHIZCwgUA21gdPgDyaJqrNHp034TAZ9KZ21rofMBjfR2RoiIPG2GA7dBD1FdQBnYGXybMWOo-vjvtF8u3j9fpqma4-39xeXa7SOheKpYWoeNbGimdl0UANNWvzMqcZiqpsqrbAilVtbLaNVKqAVlWlZAJ5neWlEkV2kbydfTfQ6QdnenAHbcHo5eVKT2eUUs4LqfYssm9m9sHZXzv0QffG19h1MKDdeS2kEFSp_J8gU5ES2TS9nMH4K947bE9XYFRPUeqtnlLSU2J6ilL_iVKPUfr6OGNX9dj8FR6zi8CHGXg0HR7-21hff7qNRZSns9z4gONJDu5nfGcmC31_d6O_3H8tl0ze6e_Zb828vK8</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Chanal, Angélique</creator><creator>Chapon, Virginie</creator><creator>Benzerara, Karim</creator><creator>Barakat, Mohamed</creator><creator>Christen, Richard</creator><creator>Achouak, Wafa</creator><creator>Barras, Frédéric</creator><creator>Heulin, Thierry</creator><general>Blackwell Publishing Ltd</general><general>Society for Applied Microbiology and Wiley-Blackwell</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-0553-0137</orcidid><orcidid>https://orcid.org/0000-0003-4297-6661</orcidid><orcidid>https://orcid.org/0000-0002-2365-3464</orcidid><orcidid>https://orcid.org/0000-0003-3458-2574</orcidid></search><sort><creationdate>200603</creationdate><title>The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria</title><author>Chanal, Angélique ; Chapon, Virginie ; Benzerara, Karim ; Barakat, Mohamed ; Christen, Richard ; Achouak, Wafa ; Barras, Frédéric ; Heulin, Thierry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4691-56b23f6912385dacac1f48403e6b8dbf5eb1bf238fd7995af9b8716e2c3489653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Actinobacteria</topic><topic>Alphaproteobacteria</topic><topic>Archaea</topic><topic>Archaea - classification</topic><topic>Archaea - genetics</topic><topic>Archaea - isolation & purification</topic><topic>Bacillus</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - radiation effects</topic><topic>Biodiversity</topic><topic>Crenarchaeota</topic><topic>Deinococcus radiodurans</topic><topic>Desert Climate</topic><topic>Desiccation</topic><topic>DNA, Archaeal - chemistry</topic><topic>DNA, Archaeal - genetics</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Ecosystem</topic><topic>Firmicutes</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Proteobacteria</topic><topic>Radiation, Ionizing</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Soil Microbiology</topic><topic>Tunisia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chanal, Angélique</creatorcontrib><creatorcontrib>Chapon, Virginie</creatorcontrib><creatorcontrib>Benzerara, Karim</creatorcontrib><creatorcontrib>Barakat, Mohamed</creatorcontrib><creatorcontrib>Christen, Richard</creatorcontrib><creatorcontrib>Achouak, Wafa</creatorcontrib><creatorcontrib>Barras, Frédéric</creatorcontrib><creatorcontrib>Heulin, Thierry</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chanal, Angélique</au><au>Chapon, Virginie</au><au>Benzerara, Karim</au><au>Barakat, Mohamed</au><au>Christen, Richard</au><au>Achouak, Wafa</au><au>Barras, Frédéric</au><au>Heulin, Thierry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2006-03</date><risdate>2006</risdate><volume>8</volume><issue>3</issue><spage>514</spage><epage>525</epage><pages>514-525</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>Summary
The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and – molecular‐based analysis. Thirty‐one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria, Firmicutes, Proteobacteria and the CFB group while none related to Archaea. Analysis of 16S rRNA gene libraries revealed the presence of species related to Bacteria and Archaea. Sequences related to Archaea were all affiliated to the non‐thermophilic Crenarchaeota subgroup. Bacterial sequences were dominated by Proteobacteria, Actinobacteria and Acidobacteria; a few sequences were distributed among eight others phyla, including Thermus/Deinococcus relatives. A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans. Because bacteria living in the hot desert of Tataouine are one way or another tolerant to desiccation, we investigate whether they could also be tolerant to radiation. Exposition of soil samples to intense gamma radiation yields Bacillus, Thermus/Deinococcus and α‐Proteobacteria relatives. Four of these strains correspond to radiotolerant species as revealed by evaluation of the resistance levels of the individual cultures. A detailed analysis of the resistance levels for two Thermus/Deinococcus and two α‐Proteobacteria relatives revealed that they correspond to new radiotolerant species.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16478457</pmid><doi>10.1111/j.1462-2920.2005.00921.x</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0553-0137</orcidid><orcidid>https://orcid.org/0000-0003-4297-6661</orcidid><orcidid>https://orcid.org/0000-0002-2365-3464</orcidid><orcidid>https://orcid.org/0000-0003-3458-2574</orcidid></addata></record> |
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| ispartof | Environmental microbiology, 2006-03, Vol.8 (3), p.514-525 |
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| subjects | Actinobacteria Alphaproteobacteria Archaea Archaea - classification Archaea - genetics Archaea - isolation & purification Bacillus Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteria - radiation effects Biodiversity Crenarchaeota Deinococcus radiodurans Desert Climate Desiccation DNA, Archaeal - chemistry DNA, Archaeal - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Ecosystem Firmicutes Molecular Sequence Data Phylogeny Proteobacteria Radiation, Ionizing RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Soil Microbiology Tunisia |
| title | The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria |
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