Recruitment of Drosophila polycomb group proteins to chromatin by DSP1
Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitmen...
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creator | Dejardin, J Rappailles, A Cuvier, O Grimaud, C Decoville, M Locker, D Cavalli, G |
description | Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We previously characterized a minimal element from the regulatory region of the Abdominal-B gene, termed Ab-Fab. Ab-Fab contains a PRE and a TRE and is able to maintain repressed or active chromatin states during development. Here we show that the Dorsal switch protein 1 (DSP1), a Drosophila HMGB2 homologue, binds to a sequence present within Ab-Fab and in other characterized PREs. Addition of this motif to an artificial sequence containing Pleiohomeotic and GAGA factor consensus sites is sufficient for PcG protein recruitment in vivo. Mutations that abolish DSP1 binding to Ab-Fab and to a PRE from the engrailed locus lead to loss of PcG protein binding, loss of silencing, and switching of these PREs into constitutive TREs. The binding of DSP1 to PREs is therefore important for the recruitment of PcG proteins. |
doi_str_mv | 10.1038/nature03386 |
format | Article |
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In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We previously characterized a minimal element from the regulatory region of the Abdominal-B gene, termed Ab-Fab. Ab-Fab contains a PRE and a TRE and is able to maintain repressed or active chromatin states during development. Here we show that the Dorsal switch protein 1 (DSP1), a Drosophila HMGB2 homologue, binds to a sequence present within Ab-Fab and in other characterized PREs. Addition of this motif to an artificial sequence containing Pleiohomeotic and GAGA factor consensus sites is sufficient for PcG protein recruitment in vivo. Mutations that abolish DSP1 binding to Ab-Fab and to a PRE from the engrailed locus lead to loss of PcG protein binding, loss of silencing, and switching of these PREs into constitutive TREs. The binding of DSP1 to PREs is therefore important for the recruitment of PcG proteins.</description><identifier>ISSN: 0028-0836</identifier><identifier>ISSN: 0767-0974</identifier><identifier>EISSN: 1476-4687</identifier><identifier>EISSN: 1958-5381</identifier><identifier>DOI: 10.1038/nature03386</identifier><identifier>PMID: 15791260</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Base Sequence ; binding capacity ; Biological and medical sciences ; chromatin ; Chromatin - genetics ; Chromatin - metabolism ; Chromatin Immunoprecipitation ; Chromatin. Chromosome ; Chromosomes - genetics ; Chromosomes - metabolism ; Consensus Sequence - genetics ; Deoxyribonucleic acid ; DNA ; DNA-binding proteins ; DNA-Binding Proteins - metabolism ; Dorsal switch protein 1 ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - embryology ; Drosophila melanogaster - genetics ; Drosophila melanogaster - metabolism ; Drosophila Proteins - classification ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; Gene Expression Regulation, Developmental ; Gene Silencing ; High Mobility Group Proteins - metabolism ; Homeodomain Proteins - genetics ; Humanities and Social Sciences ; In Situ Hybridization, Fluorescence ; Insects ; letter ; Life Sciences ; Molecular and cellular biology ; Molecular genetics ; multidisciplinary ; mutants ; Mutation ; Mutation - genetics ; Other ; Polycomb Repressive Complex 1 ; Polycomb response element ; promoter regions ; Protein Binding ; Proteins ; Response Elements - genetics ; Science ; Science (multidisciplinary) ; transcription factors ; Transcription Factors - metabolism ; Transgenes - genetics ; trithorax response element</subject><ispartof>M.S. Médecine sciences, 2005-03, Vol.434 (7032), p.533-538</ispartof><rights>Macmillan Magazines Ltd. 2005</rights><rights>2005 INIST-CNRS</rights><rights>COPYRIGHT 2005 Nature Publishing Group</rights><rights>Copyright Macmillan Journals Ltd. Mar 24, 2005</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c742t-3895f3d75ffd417656713f1b93b07cf83479e23b41e455632a7ee5444169284f3</citedby><cites>FETCH-LOGICAL-c742t-3895f3d75ffd417656713f1b93b07cf83479e23b41e455632a7ee5444169284f3</cites><orcidid>0000-0003-3709-3469</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nature03386$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nature03386$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16648089$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15791260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00016621$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Dejardin, J</creatorcontrib><creatorcontrib>Rappailles, A</creatorcontrib><creatorcontrib>Cuvier, O</creatorcontrib><creatorcontrib>Grimaud, C</creatorcontrib><creatorcontrib>Decoville, M</creatorcontrib><creatorcontrib>Locker, D</creatorcontrib><creatorcontrib>Cavalli, G</creatorcontrib><title>Recruitment of Drosophila polycomb group proteins to chromatin by DSP1</title><title>M.S. Médecine sciences</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We previously characterized a minimal element from the regulatory region of the Abdominal-B gene, termed Ab-Fab. Ab-Fab contains a PRE and a TRE and is able to maintain repressed or active chromatin states during development. Here we show that the Dorsal switch protein 1 (DSP1), a Drosophila HMGB2 homologue, binds to a sequence present within Ab-Fab and in other characterized PREs. Addition of this motif to an artificial sequence containing Pleiohomeotic and GAGA factor consensus sites is sufficient for PcG protein recruitment in vivo. Mutations that abolish DSP1 binding to Ab-Fab and to a PRE from the engrailed locus lead to loss of PcG protein binding, loss of silencing, and switching of these PREs into constitutive TREs. The binding of DSP1 to PREs is therefore important for the recruitment of PcG proteins.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>binding capacity</subject><subject>Biological and medical sciences</subject><subject>chromatin</subject><subject>Chromatin - genetics</subject><subject>Chromatin - metabolism</subject><subject>Chromatin Immunoprecipitation</subject><subject>Chromatin. 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Médecine sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dejardin, J</au><au>Rappailles, A</au><au>Cuvier, O</au><au>Grimaud, C</au><au>Decoville, M</au><au>Locker, D</au><au>Cavalli, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recruitment of Drosophila polycomb group proteins to chromatin by DSP1</atitle><jtitle>M.S. Médecine sciences</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2005-03-24</date><risdate>2005</risdate><volume>434</volume><issue>7032</issue><spage>533</spage><epage>538</epage><pages>533-538</pages><issn>0028-0836</issn><issn>0767-0974</issn><eissn>1476-4687</eissn><eissn>1958-5381</eissn><coden>NATUAS</coden><abstract>Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We previously characterized a minimal element from the regulatory region of the Abdominal-B gene, termed Ab-Fab. Ab-Fab contains a PRE and a TRE and is able to maintain repressed or active chromatin states during development. Here we show that the Dorsal switch protein 1 (DSP1), a Drosophila HMGB2 homologue, binds to a sequence present within Ab-Fab and in other characterized PREs. Addition of this motif to an artificial sequence containing Pleiohomeotic and GAGA factor consensus sites is sufficient for PcG protein recruitment in vivo. Mutations that abolish DSP1 binding to Ab-Fab and to a PRE from the engrailed locus lead to loss of PcG protein binding, loss of silencing, and switching of these PREs into constitutive TREs. The binding of DSP1 to PREs is therefore important for the recruitment of PcG proteins.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>15791260</pmid><doi>10.1038/nature03386</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-3709-3469</orcidid></addata></record> |
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identifier | ISSN: 0028-0836 |
ispartof | M.S. Médecine sciences, 2005-03, Vol.434 (7032), p.533-538 |
issn | 0028-0836 0767-0974 1476-4687 1958-5381 |
language | eng |
recordid | cdi_hal_primary_oai_HAL_hal_00016621v1 |
source | Érudit (Freely Accessible); Érudit; MEDLINE; SpringerLink Journals; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
subjects | Animals Base Sequence binding capacity Biological and medical sciences chromatin Chromatin - genetics Chromatin - metabolism Chromatin Immunoprecipitation Chromatin. Chromosome Chromosomes - genetics Chromosomes - metabolism Consensus Sequence - genetics Deoxyribonucleic acid DNA DNA-binding proteins DNA-Binding Proteins - metabolism Dorsal switch protein 1 Drosophila Drosophila melanogaster Drosophila melanogaster - embryology Drosophila melanogaster - genetics Drosophila melanogaster - metabolism Drosophila Proteins - classification Drosophila Proteins - genetics Drosophila Proteins - metabolism Fundamental and applied biological sciences. Psychology gene expression regulation Gene Expression Regulation, Developmental Gene Silencing High Mobility Group Proteins - metabolism Homeodomain Proteins - genetics Humanities and Social Sciences In Situ Hybridization, Fluorescence Insects letter Life Sciences Molecular and cellular biology Molecular genetics multidisciplinary mutants Mutation Mutation - genetics Other Polycomb Repressive Complex 1 Polycomb response element promoter regions Protein Binding Proteins Response Elements - genetics Science Science (multidisciplinary) transcription factors Transcription Factors - metabolism Transgenes - genetics trithorax response element |
title | Recruitment of Drosophila polycomb group proteins to chromatin by DSP1 |
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