Recruitment of Drosophila polycomb group proteins to chromatin by DSP1

Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitmen...

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Veröffentlicht in:M.S. Médecine sciences 2005-03, Vol.434 (7032), p.533-538
Hauptverfasser: Dejardin, J, Rappailles, A, Cuvier, O, Grimaud, C, Decoville, M, Locker, D, Cavalli, G
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container_end_page 538
container_issue 7032
container_start_page 533
container_title M.S. Médecine sciences
container_volume 434
creator Dejardin, J
Rappailles, A
Cuvier, O
Grimaud, C
Decoville, M
Locker, D
Cavalli, G
description Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We previously characterized a minimal element from the regulatory region of the Abdominal-B gene, termed Ab-Fab. Ab-Fab contains a PRE and a TRE and is able to maintain repressed or active chromatin states during development. Here we show that the Dorsal switch protein 1 (DSP1), a Drosophila HMGB2 homologue, binds to a sequence present within Ab-Fab and in other characterized PREs. Addition of this motif to an artificial sequence containing Pleiohomeotic and GAGA factor consensus sites is sufficient for PcG protein recruitment in vivo. Mutations that abolish DSP1 binding to Ab-Fab and to a PRE from the engrailed locus lead to loss of PcG protein binding, loss of silencing, and switching of these PREs into constitutive TREs. The binding of DSP1 to PREs is therefore important for the recruitment of PcG proteins.
doi_str_mv 10.1038/nature03386
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Psychology ; gene expression regulation ; Gene Expression Regulation, Developmental ; Gene Silencing ; High Mobility Group Proteins - metabolism ; Homeodomain Proteins - genetics ; Humanities and Social Sciences ; In Situ Hybridization, Fluorescence ; Insects ; letter ; Life Sciences ; Molecular and cellular biology ; Molecular genetics ; multidisciplinary ; mutants ; Mutation ; Mutation - genetics ; Other ; Polycomb Repressive Complex 1 ; Polycomb response element ; promoter regions ; Protein Binding ; Proteins ; Response Elements - genetics ; Science ; Science (multidisciplinary) ; transcription factors ; Transcription Factors - metabolism ; Transgenes - genetics ; trithorax response element</subject><ispartof>M.S. Médecine sciences, 2005-03, Vol.434 (7032), p.533-538</ispartof><rights>Macmillan Magazines Ltd. 2005</rights><rights>2005 INIST-CNRS</rights><rights>COPYRIGHT 2005 Nature Publishing Group</rights><rights>Copyright Macmillan Journals Ltd. 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Médecine sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dejardin, J</au><au>Rappailles, A</au><au>Cuvier, O</au><au>Grimaud, C</au><au>Decoville, M</au><au>Locker, D</au><au>Cavalli, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recruitment of Drosophila polycomb group proteins to chromatin by DSP1</atitle><jtitle>M.S. Médecine sciences</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2005-03-24</date><risdate>2005</risdate><volume>434</volume><issue>7032</issue><spage>533</spage><epage>538</epage><pages>533-538</pages><issn>0028-0836</issn><issn>0767-0974</issn><eissn>1476-4687</eissn><eissn>1958-5381</eissn><coden>NATUAS</coden><abstract>Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We previously characterized a minimal element from the regulatory region of the Abdominal-B gene, termed Ab-Fab. Ab-Fab contains a PRE and a TRE and is able to maintain repressed or active chromatin states during development. Here we show that the Dorsal switch protein 1 (DSP1), a Drosophila HMGB2 homologue, binds to a sequence present within Ab-Fab and in other characterized PREs. Addition of this motif to an artificial sequence containing Pleiohomeotic and GAGA factor consensus sites is sufficient for PcG protein recruitment in vivo. Mutations that abolish DSP1 binding to Ab-Fab and to a PRE from the engrailed locus lead to loss of PcG protein binding, loss of silencing, and switching of these PREs into constitutive TREs. The binding of DSP1 to PREs is therefore important for the recruitment of PcG proteins.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>15791260</pmid><doi>10.1038/nature03386</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-3709-3469</orcidid></addata></record>
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identifier ISSN: 0028-0836
ispartof M.S. Médecine sciences, 2005-03, Vol.434 (7032), p.533-538
issn 0028-0836
0767-0974
1476-4687
1958-5381
language eng
recordid cdi_hal_primary_oai_HAL_hal_00016621v1
source Érudit (Freely Accessible); Érudit; MEDLINE; SpringerLink Journals; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Animals
Base Sequence
binding capacity
Biological and medical sciences
chromatin
Chromatin - genetics
Chromatin - metabolism
Chromatin Immunoprecipitation
Chromatin. Chromosome
Chromosomes - genetics
Chromosomes - metabolism
Consensus Sequence - genetics
Deoxyribonucleic acid
DNA
DNA-binding proteins
DNA-Binding Proteins - metabolism
Dorsal switch protein 1
Drosophila
Drosophila melanogaster
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - classification
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Fundamental and applied biological sciences. Psychology
gene expression regulation
Gene Expression Regulation, Developmental
Gene Silencing
High Mobility Group Proteins - metabolism
Homeodomain Proteins - genetics
Humanities and Social Sciences
In Situ Hybridization, Fluorescence
Insects
letter
Life Sciences
Molecular and cellular biology
Molecular genetics
multidisciplinary
mutants
Mutation
Mutation - genetics
Other
Polycomb Repressive Complex 1
Polycomb response element
promoter regions
Protein Binding
Proteins
Response Elements - genetics
Science
Science (multidisciplinary)
transcription factors
Transcription Factors - metabolism
Transgenes - genetics
trithorax response element
title Recruitment of Drosophila polycomb group proteins to chromatin by DSP1
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