Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking
Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2003-10, Vol.302 (5644), p.442-445 |
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| creator | Dahan, Maxime Lévi, Sabine Luccardini, Camilla Rostaing, Philippe Riveau, Béatrice Triller, Antoine |
| description | Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors. |
| doi_str_mv | 10.1126/science.1088525 |
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We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Biological Physics</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell membranes</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. 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Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking</title><author>Dahan, Maxime ; Lévi, Sabine ; Luccardini, Camilla ; Rostaing, Philippe ; Riveau, Béatrice ; Triller, Antoine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c815t-8667945c415ba7058bde31a03efa676266e5594104954768f6ac3c89678613183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Biological Physics</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell membranes</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>Central neurotransmission. 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We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>14564008</pmid><doi>10.1126/science.1088525</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-7530-1233</orcidid><orcidid>https://orcid.org/0000-0002-9927-0945</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2003-10, Vol.302 (5644), p.442-445 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00002449v1 |
| source | MEDLINE; Science Magazine; JSTOR Archive Collection A-Z Listing |
| subjects | Animals Antibodies Biological and medical sciences Biological Physics Cell Membrane - metabolism Cell Membrane - ultrastructure Cell membranes Cells, Cultured Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Dendrites - metabolism Dendrites - ultrastructure Diffusion Diffusion coefficient Fluorescence Fluorescence spectroscopy Fluorescent Dyes Fractions Fundamental and applied biological sciences. Psychology Glycine Measurement Medical imaging Microscopy, Electron Molecules Nanotechnology Neurites - metabolism Neurites - ultrastructure Neurons Neurons - metabolism Neurons - ultrastructure Neuroscience Observation Physics Physiological aspects Pyridinium Compounds Quaternary Ammonium Compounds Rats Rats, Sprague-Dawley Receptors Receptors, Glycine - metabolism Semiconductors Spinal Cord - cytology Synapses Synapses - metabolism Synapses - ultrastructure Trajectories Vertebrates: nervous system and sense organs |
| title | Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking |
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