Expression of HLA-G in human cornea, an immune-privileged tissue
Human leukocyte antigen (HLA)-G retains the capacity to modulate immune responses, favoring the establishment of tolerance in solid-tissue allotransplants. To better understand the mechanisms that promote corneal allograft survival, we investigated whether HLA-G was an immunoregulatory factor involv...
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Veröffentlicht in: | Human immunology 2003-11, Vol.64 (11), p.1039-1044 |
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description | Human leukocyte antigen (HLA)-G retains the capacity to modulate immune responses, favoring the establishment of tolerance in solid-tissue allotransplants. To better understand the mechanisms that promote corneal allograft survival, we investigated whether HLA-G was an immunoregulatory factor involved in corneal immunology. We therefore sought HLA-G expression in corneal tissues. Corneal transplantation consists in replacing the center of a diseased cornea with normal corneal tissue. Two corneal parts are not used in such surgery: diseased central corneal tissue and peripheral normal cornea. For this study, we used healthy corneas obtained from deceased donors and diseased corneas obtained from patients with pseudophakic bullous keratopathy or keratoconus who had undergone corneal transplantation. Immunohistochemical analysis carried out on the cryopreserved corneas showed a positive immunohistochemical staining with anti–HLA-G, anti–HLA-A, -B, and -C, and anti–HLA class I monoclonal antibodies. Staining was obtained for keratocytes, epithelial cells, and endothelial cells from both healthy and pathologic human corneas, revealing the presence of HLA class I proteins, including HLA-G. HLA-G transcripts were detected in normal cornea by reverse transcriptase–polymerase chain reaction with a classical pattern of alternative splicing. The detection of HLA-G protein in adult corneas leads to the conclusion that this protein may contribute to the maintenance of the privileged immune status of cornea. |
doi_str_mv | 10.1016/j.humimm.2003.08.346 |
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To better understand the mechanisms that promote corneal allograft survival, we investigated whether HLA-G was an immunoregulatory factor involved in corneal immunology. We therefore sought HLA-G expression in corneal tissues. Corneal transplantation consists in replacing the center of a diseased cornea with normal corneal tissue. Two corneal parts are not used in such surgery: diseased central corneal tissue and peripheral normal cornea. For this study, we used healthy corneas obtained from deceased donors and diseased corneas obtained from patients with pseudophakic bullous keratopathy or keratoconus who had undergone corneal transplantation. Immunohistochemical analysis carried out on the cryopreserved corneas showed a positive immunohistochemical staining with anti–HLA-G, anti–HLA-A, -B, and -C, and anti–HLA class I monoclonal antibodies. Staining was obtained for keratocytes, epithelial cells, and endothelial cells from both healthy and pathologic human corneas, revealing the presence of HLA class I proteins, including HLA-G. HLA-G transcripts were detected in normal cornea by reverse transcriptase–polymerase chain reaction with a classical pattern of alternative splicing. The detection of HLA-G protein in adult corneas leads to the conclusion that this protein may contribute to the maintenance of the privileged immune status of cornea.</description><identifier>ISSN: 0198-8859</identifier><identifier>EISSN: 1879-1166</identifier><identifier>DOI: 10.1016/j.humimm.2003.08.346</identifier><identifier>PMID: 14602233</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Cornea ; Cornea - cytology ; Cornea - immunology ; Corneal Diseases ; Corneal Diseases - immunology ; Corneal Transplantation ; Fluorescent Antibody Technique ; Histocompatibility Antigens Class I ; Histocompatibility Antigens Class I - analysis ; Histocompatibility Antigens Class I - genetics ; Histocompatibility Antigens Class I - immunology ; HLA ; HLA Antigens ; HLA Antigens - analysis ; HLA Antigens - genetics ; HLA Antigens - immunology ; HLA-A Antigens ; HLA-A Antigens - analysis ; HLA-B Antigens ; HLA-B Antigens - immunology ; HLA-C Antigens ; HLA-C Antigens - analysis ; HLA-G Antigens ; Humans ; Immunohistochemistry ; Immunology ; Keratoconus ; Keratoconus - immunology ; keratoplasty ; Life Sciences ; Pseudophakia ; Pseudophakia - immunology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; RNA - analysis ; RT-PCR</subject><ispartof>Human immunology, 2003-11, Vol.64 (11), p.1039-1044</ispartof><rights>2003 American Society for Histocompatibility and Immunogenetics</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-8e71c83e2b5b2af3e665451ebdfc70ff151e2908b9554d75623366b0747c12d03</citedby><cites>FETCH-LOGICAL-c469t-8e71c83e2b5b2af3e665451ebdfc70ff151e2908b9554d75623366b0747c12d03</cites><orcidid>0000-0001-5126-0900 ; 0000-0003-1780-8746</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.humimm.2003.08.346$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14602233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://cea.hal.science/cea-00273197$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Le Discorde, Magali</creatorcontrib><creatorcontrib>Moreau, Philippe</creatorcontrib><creatorcontrib>Sabatier, Patrick</creatorcontrib><creatorcontrib>Legeais, Jean-Marc</creatorcontrib><creatorcontrib>Carosella, Edgardo D</creatorcontrib><title>Expression of HLA-G in human cornea, an immune-privileged tissue</title><title>Human immunology</title><addtitle>Hum Immunol</addtitle><description>Human leukocyte antigen (HLA)-G retains the capacity to modulate immune responses, favoring the establishment of tolerance in solid-tissue allotransplants. To better understand the mechanisms that promote corneal allograft survival, we investigated whether HLA-G was an immunoregulatory factor involved in corneal immunology. We therefore sought HLA-G expression in corneal tissues. Corneal transplantation consists in replacing the center of a diseased cornea with normal corneal tissue. Two corneal parts are not used in such surgery: diseased central corneal tissue and peripheral normal cornea. For this study, we used healthy corneas obtained from deceased donors and diseased corneas obtained from patients with pseudophakic bullous keratopathy or keratoconus who had undergone corneal transplantation. Immunohistochemical analysis carried out on the cryopreserved corneas showed a positive immunohistochemical staining with anti–HLA-G, anti–HLA-A, -B, and -C, and anti–HLA class I monoclonal antibodies. Staining was obtained for keratocytes, epithelial cells, and endothelial cells from both healthy and pathologic human corneas, revealing the presence of HLA class I proteins, including HLA-G. HLA-G transcripts were detected in normal cornea by reverse transcriptase–polymerase chain reaction with a classical pattern of alternative splicing. The detection of HLA-G protein in adult corneas leads to the conclusion that this protein may contribute to the maintenance of the privileged immune status of cornea.</description><subject>Adult</subject><subject>Cornea</subject><subject>Cornea - cytology</subject><subject>Cornea - immunology</subject><subject>Corneal Diseases</subject><subject>Corneal Diseases - immunology</subject><subject>Corneal Transplantation</subject><subject>Fluorescent Antibody Technique</subject><subject>Histocompatibility Antigens Class I</subject><subject>Histocompatibility Antigens Class I - analysis</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>Histocompatibility Antigens Class I - immunology</subject><subject>HLA</subject><subject>HLA Antigens</subject><subject>HLA Antigens - analysis</subject><subject>HLA Antigens - genetics</subject><subject>HLA Antigens - immunology</subject><subject>HLA-A Antigens</subject><subject>HLA-A Antigens - analysis</subject><subject>HLA-B Antigens</subject><subject>HLA-B Antigens - immunology</subject><subject>HLA-C Antigens</subject><subject>HLA-C Antigens - analysis</subject><subject>HLA-G Antigens</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunology</subject><subject>Keratoconus</subject><subject>Keratoconus - immunology</subject><subject>keratoplasty</subject><subject>Life Sciences</subject><subject>Pseudophakia</subject><subject>Pseudophakia - immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA</subject><subject>RNA - analysis</subject><subject>RT-PCR</subject><issn>0198-8859</issn><issn>1879-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxYMo7rj6H4j0SRDstirpzsdFdljWHWHAi55DOl3tZuiPMZke9L83Sw9601PV4VevHu8x9hqhQkD54VA9LGMYx4oDiAp0JWr5hG1QK1MiSvmUbQCNLrVuzBV7kdIBABSo-jm7wloC50Js2M3dz2OklMI8FXNf7Pbb8r4IU5G13VT4OU7k3hd5zZ-WicpjDOcw0HfqilNIaaGX7FnvhkSvLvOafft09_V2V-6_3H--3e5LX0tzKjUp9FoQb5uWu16QlE3dILVd7xX0PeadG9CtaZq6U43M7qRss13lkXcgrtm7VffBDTa7GF38ZWcX7G67t56cBeBKoFFnzOzblT3G-cdC6WTHkDwNg5toXpJVKIThgv8XRIO1VkJlsF5BH-eUIvV_LCDYxzrswa512Mc6LGib68hnby76SztS9_fokn8GPq4A5ejOgaJNPtDkqQuR_Ml2c_j3h98HkJqD</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Le Discorde, Magali</creator><creator>Moreau, Philippe</creator><creator>Sabatier, Patrick</creator><creator>Legeais, Jean-Marc</creator><creator>Carosella, Edgardo D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-5126-0900</orcidid><orcidid>https://orcid.org/0000-0003-1780-8746</orcidid></search><sort><creationdate>20031101</creationdate><title>Expression of HLA-G in human cornea, an immune-privileged tissue</title><author>Le Discorde, Magali ; Moreau, Philippe ; Sabatier, Patrick ; Legeais, Jean-Marc ; Carosella, Edgardo D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-8e71c83e2b5b2af3e665451ebdfc70ff151e2908b9554d75623366b0747c12d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Cornea</topic><topic>Cornea - cytology</topic><topic>Cornea - immunology</topic><topic>Corneal Diseases</topic><topic>Corneal Diseases - immunology</topic><topic>Corneal Transplantation</topic><topic>Fluorescent Antibody Technique</topic><topic>Histocompatibility Antigens Class I</topic><topic>Histocompatibility Antigens Class I - analysis</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>Histocompatibility Antigens Class I - immunology</topic><topic>HLA</topic><topic>HLA Antigens</topic><topic>HLA Antigens - analysis</topic><topic>HLA Antigens - genetics</topic><topic>HLA Antigens - immunology</topic><topic>HLA-A Antigens</topic><topic>HLA-A Antigens - analysis</topic><topic>HLA-B Antigens</topic><topic>HLA-B Antigens - immunology</topic><topic>HLA-C Antigens</topic><topic>HLA-C Antigens - analysis</topic><topic>HLA-G Antigens</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunology</topic><topic>Keratoconus</topic><topic>Keratoconus - immunology</topic><topic>keratoplasty</topic><topic>Life Sciences</topic><topic>Pseudophakia</topic><topic>Pseudophakia - immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA</topic><topic>RNA - analysis</topic><topic>RT-PCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Le Discorde, Magali</creatorcontrib><creatorcontrib>Moreau, Philippe</creatorcontrib><creatorcontrib>Sabatier, Patrick</creatorcontrib><creatorcontrib>Legeais, Jean-Marc</creatorcontrib><creatorcontrib>Carosella, Edgardo D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Human immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Le Discorde, Magali</au><au>Moreau, Philippe</au><au>Sabatier, Patrick</au><au>Legeais, Jean-Marc</au><au>Carosella, Edgardo D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of HLA-G in human cornea, an immune-privileged tissue</atitle><jtitle>Human immunology</jtitle><addtitle>Hum Immunol</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>64</volume><issue>11</issue><spage>1039</spage><epage>1044</epage><pages>1039-1044</pages><issn>0198-8859</issn><eissn>1879-1166</eissn><abstract>Human leukocyte antigen (HLA)-G retains the capacity to modulate immune responses, favoring the establishment of tolerance in solid-tissue allotransplants. To better understand the mechanisms that promote corneal allograft survival, we investigated whether HLA-G was an immunoregulatory factor involved in corneal immunology. We therefore sought HLA-G expression in corneal tissues. Corneal transplantation consists in replacing the center of a diseased cornea with normal corneal tissue. Two corneal parts are not used in such surgery: diseased central corneal tissue and peripheral normal cornea. For this study, we used healthy corneas obtained from deceased donors and diseased corneas obtained from patients with pseudophakic bullous keratopathy or keratoconus who had undergone corneal transplantation. Immunohistochemical analysis carried out on the cryopreserved corneas showed a positive immunohistochemical staining with anti–HLA-G, anti–HLA-A, -B, and -C, and anti–HLA class I monoclonal antibodies. Staining was obtained for keratocytes, epithelial cells, and endothelial cells from both healthy and pathologic human corneas, revealing the presence of HLA class I proteins, including HLA-G. HLA-G transcripts were detected in normal cornea by reverse transcriptase–polymerase chain reaction with a classical pattern of alternative splicing. The detection of HLA-G protein in adult corneas leads to the conclusion that this protein may contribute to the maintenance of the privileged immune status of cornea.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14602233</pmid><doi>10.1016/j.humimm.2003.08.346</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5126-0900</orcidid><orcidid>https://orcid.org/0000-0003-1780-8746</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Adult Cornea Cornea - cytology Cornea - immunology Corneal Diseases Corneal Diseases - immunology Corneal Transplantation Fluorescent Antibody Technique Histocompatibility Antigens Class I Histocompatibility Antigens Class I - analysis Histocompatibility Antigens Class I - genetics Histocompatibility Antigens Class I - immunology HLA HLA Antigens HLA Antigens - analysis HLA Antigens - genetics HLA Antigens - immunology HLA-A Antigens HLA-A Antigens - analysis HLA-B Antigens HLA-B Antigens - immunology HLA-C Antigens HLA-C Antigens - analysis HLA-G Antigens Humans Immunohistochemistry Immunology Keratoconus Keratoconus - immunology keratoplasty Life Sciences Pseudophakia Pseudophakia - immunology Reverse Transcriptase Polymerase Chain Reaction RNA RNA - analysis RT-PCR |
title | Expression of HLA-G in human cornea, an immune-privileged tissue |
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