Quantification of indicator and pathogenic bacteria in manures and digestates from three agricultural biogas plants over a one-year period
•The fate of FIB and of pathogenic bacteria though AD varied with the biogas plant.•log10 reductions of pathogenic bacteria though AD varied over time.•Campylobacter and Salmonella were less persistent than L. monocytogenes.•The reduction of C. botulinum and C. difficile were less than or equal to 0...
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| Veröffentlicht in: | Waste management (Elmsford) 2023-09, Vol.169, p.91-100 |
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| creator | Pourcher, Anne-Marie Druilhe, Céline Le Maréchal, Caroline Repérant, Elisabeth Boscher, Evelyne Ziebal, Christine Martin, Laure Lebreton, Megane Rouxel, Sandra Houdayer, Catherine Le Roux, Sophie Derongs, Lorine Poëzévara, Typhaine Sarrazin, Martine Nagard, Bérengère Heurtevent, Lorette Denis, Martine |
| description | •The fate of FIB and of pathogenic bacteria though AD varied with the biogas plant.•log10 reductions of pathogenic bacteria though AD varied over time.•Campylobacter and Salmonella were less persistent than L. monocytogenes.•The reduction of C. botulinum and C. difficile were less than or equal to 0.5 log10.
Interest in the conversion of manure in biogas via anaerobic digestion (AD) is growing, but questions remain about the biosafety of digestates. For a period of one year, we monitored the impact of three mesophilic agricultural biogas plants (BPs) mainly fed with pig manure (BP1, BP3) or bovine manure (BP2) on the physicochemical parameters, the composition of the microbial community and the concentration of bacteria (E. coli, enterococci, Salmonella, Campylobacter, Listeria monocytogenes, Clostridium perfringens, Clostridium botulinum and Clostridioides difficile). The BP2 digestate differed from those of the two other BPs with a higher nitrogen content, more total solids and greater abundance of Clostridia MBA03 and Disgonomonadacea. Persistence during digestion ranked from least to most, was: Campylobacter (1.6 to >2.9 log10 reduction, according to the BP) |
| doi_str_mv | 10.1016/j.wasman.2023.06.037 |
| format | Article |
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Interest in the conversion of manure in biogas via anaerobic digestion (AD) is growing, but questions remain about the biosafety of digestates. For a period of one year, we monitored the impact of three mesophilic agricultural biogas plants (BPs) mainly fed with pig manure (BP1, BP3) or bovine manure (BP2) on the physicochemical parameters, the composition of the microbial community and the concentration of bacteria (E. coli, enterococci, Salmonella, Campylobacter, Listeria monocytogenes, Clostridium perfringens, Clostridium botulinum and Clostridioides difficile). The BP2 digestate differed from those of the two other BPs with a higher nitrogen content, more total solids and greater abundance of Clostridia MBA03 and Disgonomonadacea. Persistence during digestion ranked from least to most, was: Campylobacter (1.6 to >2.9 log10 reduction, according to the BP) < E. coli (1.8 to 2.2 log10) < Salmonella (1.1 to 1.4 log10) < enterococci (0.2 to 1.2 log10) and C. perfringens (0.2 to 1 log10) < L. monocytogenes (-1.2 to 1.6 log10) < C. difficile and C. botulinum (≤0.5 log10). No statistical link was found between the reduction in the concentration of the targeted bacteria and the physicochemical and operational parameters likely to have an effect (NH3, volatile fatty acids and total solids contents, hydraulic retention time, presence of co-substrates), underlining the fact that the fate of the bacteria during mesophilic digestion depends on many interacting factors. The reduction in concentrations varied significantly over the sampling period, underlining the need for longitudinal studies to estimate the impact of AD on pathogenic microorganisms.</description><identifier>ISSN: 0956-053X</identifier><identifier>EISSN: 1879-2456</identifier><identifier>DOI: 10.1016/j.wasman.2023.06.037</identifier><identifier>PMID: 37418788</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Digestate ; Fecal indicator bacteria ; Life Sciences ; Manure ; Mesophilic biogas plant ; Microbial communities ; Pathogenic bacteria</subject><ispartof>Waste management (Elmsford), 2023-09, Vol.169, p.91-100</ispartof><rights>2023 Elsevier Ltd</rights><rights>Copyright © 2023 Elsevier Ltd. All rights reserved.</rights><rights>Copyright</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c347t-8a3984e950b1f5acad4703b6b81144a5297dbcf95f478ec580880d260c39822d3</cites><orcidid>0000-0001-5615-7718 ; 0000-0002-5056-4918 ; 0009-0002-0961-7034 ; 0009-0000-3309-7847 ; 0000-0002-0950-2256</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.wasman.2023.06.037$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,782,786,887,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37418788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://anses.hal.science/anses-04170587$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pourcher, Anne-Marie</creatorcontrib><creatorcontrib>Druilhe, Céline</creatorcontrib><creatorcontrib>Le Maréchal, Caroline</creatorcontrib><creatorcontrib>Repérant, Elisabeth</creatorcontrib><creatorcontrib>Boscher, Evelyne</creatorcontrib><creatorcontrib>Ziebal, Christine</creatorcontrib><creatorcontrib>Martin, Laure</creatorcontrib><creatorcontrib>Lebreton, Megane</creatorcontrib><creatorcontrib>Rouxel, Sandra</creatorcontrib><creatorcontrib>Houdayer, Catherine</creatorcontrib><creatorcontrib>Le Roux, Sophie</creatorcontrib><creatorcontrib>Derongs, Lorine</creatorcontrib><creatorcontrib>Poëzévara, Typhaine</creatorcontrib><creatorcontrib>Sarrazin, Martine</creatorcontrib><creatorcontrib>Nagard, Bérengère</creatorcontrib><creatorcontrib>Heurtevent, Lorette</creatorcontrib><creatorcontrib>Denis, Martine</creatorcontrib><title>Quantification of indicator and pathogenic bacteria in manures and digestates from three agricultural biogas plants over a one-year period</title><title>Waste management (Elmsford)</title><addtitle>Waste Manag</addtitle><description>•The fate of FIB and of pathogenic bacteria though AD varied with the biogas plant.•log10 reductions of pathogenic bacteria though AD varied over time.•Campylobacter and Salmonella were less persistent than L. monocytogenes.•The reduction of C. botulinum and C. difficile were less than or equal to 0.5 log10.
Interest in the conversion of manure in biogas via anaerobic digestion (AD) is growing, but questions remain about the biosafety of digestates. For a period of one year, we monitored the impact of three mesophilic agricultural biogas plants (BPs) mainly fed with pig manure (BP1, BP3) or bovine manure (BP2) on the physicochemical parameters, the composition of the microbial community and the concentration of bacteria (E. coli, enterococci, Salmonella, Campylobacter, Listeria monocytogenes, Clostridium perfringens, Clostridium botulinum and Clostridioides difficile). The BP2 digestate differed from those of the two other BPs with a higher nitrogen content, more total solids and greater abundance of Clostridia MBA03 and Disgonomonadacea. Persistence during digestion ranked from least to most, was: Campylobacter (1.6 to >2.9 log10 reduction, according to the BP) < E. coli (1.8 to 2.2 log10) < Salmonella (1.1 to 1.4 log10) < enterococci (0.2 to 1.2 log10) and C. perfringens (0.2 to 1 log10) < L. monocytogenes (-1.2 to 1.6 log10) < C. difficile and C. botulinum (≤0.5 log10). No statistical link was found between the reduction in the concentration of the targeted bacteria and the physicochemical and operational parameters likely to have an effect (NH3, volatile fatty acids and total solids contents, hydraulic retention time, presence of co-substrates), underlining the fact that the fate of the bacteria during mesophilic digestion depends on many interacting factors. The reduction in concentrations varied significantly over the sampling period, underlining the need for longitudinal studies to estimate the impact of AD on pathogenic microorganisms.</description><subject>Digestate</subject><subject>Fecal indicator bacteria</subject><subject>Life Sciences</subject><subject>Manure</subject><subject>Mesophilic biogas plant</subject><subject>Microbial communities</subject><subject>Pathogenic bacteria</subject><issn>0956-053X</issn><issn>1879-2456</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kVGL1DAUhYso7rj6D0TyKEjHpEmb9EVYltUVBkRQ8C3cJrczGdqmJuks-xf81Wa26z76dLnwnXO49xTFW0a3jLLm43F7B3GEaVvRim9ps6VcPis2TMm2rETdPC82tK2bktb810XxKsYjpUwoRl8WF1yKzCm1Kf58X2BKrncGkvMT8T1xkz1vPhCYLJkhHfweJ2dIByZhcJAJkoOXgPEBsW6PMUHKax_8SNIhIBLYB2eWIS0BBtI5v4dI5iGHReJPmM2Jn7C8Rwhkzq7evi5e9DBEfPM4L4ufn29-XN-Wu29fvl5f7UrDhUylAt4qgW1NO9bXYMAKSXnXdIoxIaCuWmk707d1L6RCUyuqFLVVQ03WVZXll8WH1fcAg56DGyHcaw9O317tNEwRo6aCSVoreWKZfr_Sc_C_l3ynHl00OORL0C9RV4rXlRS85RkVK2qCjzFg_-TOqD53po967UyfO9O00bmzLHv3mLB0I9on0b-SMvBpBTB_5eQw6GgcTgatC2iStt79P-Ev-g2r0w</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Pourcher, Anne-Marie</creator><creator>Druilhe, Céline</creator><creator>Le Maréchal, Caroline</creator><creator>Repérant, Elisabeth</creator><creator>Boscher, Evelyne</creator><creator>Ziebal, Christine</creator><creator>Martin, Laure</creator><creator>Lebreton, Megane</creator><creator>Rouxel, Sandra</creator><creator>Houdayer, Catherine</creator><creator>Le Roux, Sophie</creator><creator>Derongs, Lorine</creator><creator>Poëzévara, Typhaine</creator><creator>Sarrazin, Martine</creator><creator>Nagard, Bérengère</creator><creator>Heurtevent, Lorette</creator><creator>Denis, Martine</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5615-7718</orcidid><orcidid>https://orcid.org/0000-0002-5056-4918</orcidid><orcidid>https://orcid.org/0009-0002-0961-7034</orcidid><orcidid>https://orcid.org/0009-0000-3309-7847</orcidid><orcidid>https://orcid.org/0000-0002-0950-2256</orcidid></search><sort><creationdate>20230901</creationdate><title>Quantification of indicator and pathogenic bacteria in manures and digestates from three agricultural biogas plants over a one-year period</title><author>Pourcher, Anne-Marie ; 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Interest in the conversion of manure in biogas via anaerobic digestion (AD) is growing, but questions remain about the biosafety of digestates. For a period of one year, we monitored the impact of three mesophilic agricultural biogas plants (BPs) mainly fed with pig manure (BP1, BP3) or bovine manure (BP2) on the physicochemical parameters, the composition of the microbial community and the concentration of bacteria (E. coli, enterococci, Salmonella, Campylobacter, Listeria monocytogenes, Clostridium perfringens, Clostridium botulinum and Clostridioides difficile). The BP2 digestate differed from those of the two other BPs with a higher nitrogen content, more total solids and greater abundance of Clostridia MBA03 and Disgonomonadacea. Persistence during digestion ranked from least to most, was: Campylobacter (1.6 to >2.9 log10 reduction, according to the BP) < E. coli (1.8 to 2.2 log10) < Salmonella (1.1 to 1.4 log10) < enterococci (0.2 to 1.2 log10) and C. perfringens (0.2 to 1 log10) < L. monocytogenes (-1.2 to 1.6 log10) < C. difficile and C. botulinum (≤0.5 log10). No statistical link was found between the reduction in the concentration of the targeted bacteria and the physicochemical and operational parameters likely to have an effect (NH3, volatile fatty acids and total solids contents, hydraulic retention time, presence of co-substrates), underlining the fact that the fate of the bacteria during mesophilic digestion depends on many interacting factors. The reduction in concentrations varied significantly over the sampling period, underlining the need for longitudinal studies to estimate the impact of AD on pathogenic microorganisms.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>37418788</pmid><doi>10.1016/j.wasman.2023.06.037</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5615-7718</orcidid><orcidid>https://orcid.org/0000-0002-5056-4918</orcidid><orcidid>https://orcid.org/0009-0002-0961-7034</orcidid><orcidid>https://orcid.org/0009-0000-3309-7847</orcidid><orcidid>https://orcid.org/0000-0002-0950-2256</orcidid></addata></record> |
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| subjects | Digestate Fecal indicator bacteria Life Sciences Manure Mesophilic biogas plant Microbial communities Pathogenic bacteria |
| title | Quantification of indicator and pathogenic bacteria in manures and digestates from three agricultural biogas plants over a one-year period |
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