Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus

Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation req...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Calderwood, Alexander, Lloyd, Andrew, Hepworth, Jo, Tudor, Eleri H, Jones, Marc, Woodhouse, Shannon, Bilham, Lorelei, Chinoy, Catherine, Williams, Kevin, Corke, Fiona, Doonan, John H, Ostergaard, Lars, Irwin, Judith A, Wells, Rachel, Morris, Richard J
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator Calderwood, Alexander
Lloyd, Andrew
Hepworth, Jo
Tudor, Eleri H
Jones, Marc
Woodhouse, Shannon
Bilham, Lorelei
Chinoy, Catherine
Williams, Kevin
Corke, Fiona
Doonan, John H
Ostergaard, Lars
Irwin, Judith A
Wells, Rachel
Morris, Richard J
description Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation requirement as a system. We collected transcriptome time series for Brassica napus spring, winter, semi-winter, and Siberian kale crop types. Modelling was used to link FLC expression dynamics to floral response following vernalisation. We show that relaxed selection pressure has allowed expression of FLC paralogues to diverge, resulting in variation of FLC expression during cold treatment between paralogues and accessions. We find that total FLC expression dynamics best explains differences in cold requirement between cultivars, rather than expression of specific FLC paralogues. The combination of multiple FLC paralogues with different expression dynamics leads to rich behaviour in response to cold and a wide range of vernalisation requirements in B. napus. We find evidence for different strategies to determine the response to cold in existing winter rapeseed accessions.
format Article
fullrecord <record><control><sourceid>ghent</sourceid><recordid>TN_cdi_ghent_librecat_oai_archive_ugent_be_8694768</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_archive_ugent_be_8694768</sourcerecordid><originalsourceid>FETCH-ghent_librecat_oai_archive_ugent_be_86947683</originalsourceid><addsrcrecordid>eNqdjTFuAjEQRV0ECUK4w1wAyVrAWVpQUIqUFOmswQzLIGNbM94k3D6LkhNQ_V_899-TmVjbtHO3dJ9j86x6sdauV66ZmLLPFSPsPrZQBZMG4VLheEt45aBwknyFI3-RdJQqFBSMuesJ6KcIqXJO9xqR0zCO-ZuEU_dHKNcbcIKN4DAMCAlLry9mdMKoNPvPqWl2b_vt-7w7DwYf-SAUsPqM7FHCeXjy_V3uD-Rbt16-unbxEPQLRjFYnA</addsrcrecordid><sourcetype>Institutional Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>Ghent University Academic Bibliography</source><source>Wiley Online Library (Open Access Collection)</source><creator>Calderwood, Alexander ; Lloyd, Andrew ; Hepworth, Jo ; Tudor, Eleri H ; Jones, Marc ; Woodhouse, Shannon ; Bilham, Lorelei ; Chinoy, Catherine ; Williams, Kevin ; Corke, Fiona ; Doonan, John H ; Ostergaard, Lars ; Irwin, Judith A ; Wells, Rachel ; Morris, Richard J</creator><creatorcontrib>Calderwood, Alexander ; Lloyd, Andrew ; Hepworth, Jo ; Tudor, Eleri H ; Jones, Marc ; Woodhouse, Shannon ; Bilham, Lorelei ; Chinoy, Catherine ; Williams, Kevin ; Corke, Fiona ; Doonan, John H ; Ostergaard, Lars ; Irwin, Judith A ; Wells, Rachel ; Morris, Richard J</creatorcontrib><description>Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation requirement as a system. We collected transcriptome time series for Brassica napus spring, winter, semi-winter, and Siberian kale crop types. Modelling was used to link FLC expression dynamics to floral response following vernalisation. We show that relaxed selection pressure has allowed expression of FLC paralogues to diverge, resulting in variation of FLC expression during cold treatment between paralogues and accessions. We find that total FLC expression dynamics best explains differences in cold requirement between cultivars, rather than expression of specific FLC paralogues. The combination of multiple FLC paralogues with different expression dynamics leads to rich behaviour in response to cold and a wide range of vernalisation requirements in B. napus. We find evidence for different strategies to determine the response to cold in existing winter rapeseed accessions.</description><identifier>ISSN: 0028-646X</identifier><identifier>ISSN: 1469-8137</identifier><language>eng</language><subject>ARABIDOPSIS-THALIANA ; Biology and Life Sciences ; Brassica napus ; CABBAGE ; DOSAGE Author Information ; EVOLUTION ; flowering locus C ; GENE ; gene dosage balance ; LOCUS-C ; MADS DOMAIN PROTEIN ; modelling ; NATURAL VARIATION ; phenotypic plasticity ; polyploidy ; TIME ; transcriptomics ; vernalisation ; VERNALIZATION</subject><creationdate>2021</creationdate><rights>Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,316,782,786,4026,27867</link.rule.ids></links><search><creatorcontrib>Calderwood, Alexander</creatorcontrib><creatorcontrib>Lloyd, Andrew</creatorcontrib><creatorcontrib>Hepworth, Jo</creatorcontrib><creatorcontrib>Tudor, Eleri H</creatorcontrib><creatorcontrib>Jones, Marc</creatorcontrib><creatorcontrib>Woodhouse, Shannon</creatorcontrib><creatorcontrib>Bilham, Lorelei</creatorcontrib><creatorcontrib>Chinoy, Catherine</creatorcontrib><creatorcontrib>Williams, Kevin</creatorcontrib><creatorcontrib>Corke, Fiona</creatorcontrib><creatorcontrib>Doonan, John H</creatorcontrib><creatorcontrib>Ostergaard, Lars</creatorcontrib><creatorcontrib>Irwin, Judith A</creatorcontrib><creatorcontrib>Wells, Rachel</creatorcontrib><creatorcontrib>Morris, Richard J</creatorcontrib><title>Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus</title><description>Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation requirement as a system. We collected transcriptome time series for Brassica napus spring, winter, semi-winter, and Siberian kale crop types. Modelling was used to link FLC expression dynamics to floral response following vernalisation. We show that relaxed selection pressure has allowed expression of FLC paralogues to diverge, resulting in variation of FLC expression during cold treatment between paralogues and accessions. We find that total FLC expression dynamics best explains differences in cold requirement between cultivars, rather than expression of specific FLC paralogues. The combination of multiple FLC paralogues with different expression dynamics leads to rich behaviour in response to cold and a wide range of vernalisation requirements in B. napus. We find evidence for different strategies to determine the response to cold in existing winter rapeseed accessions.</description><subject>ARABIDOPSIS-THALIANA</subject><subject>Biology and Life Sciences</subject><subject>Brassica napus</subject><subject>CABBAGE</subject><subject>DOSAGE Author Information</subject><subject>EVOLUTION</subject><subject>flowering locus C</subject><subject>GENE</subject><subject>gene dosage balance</subject><subject>LOCUS-C</subject><subject>MADS DOMAIN PROTEIN</subject><subject>modelling</subject><subject>NATURAL VARIATION</subject><subject>phenotypic plasticity</subject><subject>polyploidy</subject><subject>TIME</subject><subject>transcriptomics</subject><subject>vernalisation</subject><subject>VERNALIZATION</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ADGLB</sourceid><recordid>eNqdjTFuAjEQRV0ECUK4w1wAyVrAWVpQUIqUFOmswQzLIGNbM94k3D6LkhNQ_V_899-TmVjbtHO3dJ9j86x6sdauV66ZmLLPFSPsPrZQBZMG4VLheEt45aBwknyFI3-RdJQqFBSMuesJ6KcIqXJO9xqR0zCO-ZuEU_dHKNcbcIKN4DAMCAlLry9mdMKoNPvPqWl2b_vt-7w7DwYf-SAUsPqM7FHCeXjy_V3uD-Rbt16-unbxEPQLRjFYnA</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Calderwood, Alexander</creator><creator>Lloyd, Andrew</creator><creator>Hepworth, Jo</creator><creator>Tudor, Eleri H</creator><creator>Jones, Marc</creator><creator>Woodhouse, Shannon</creator><creator>Bilham, Lorelei</creator><creator>Chinoy, Catherine</creator><creator>Williams, Kevin</creator><creator>Corke, Fiona</creator><creator>Doonan, John H</creator><creator>Ostergaard, Lars</creator><creator>Irwin, Judith A</creator><creator>Wells, Rachel</creator><creator>Morris, Richard J</creator><scope>ADGLB</scope></search><sort><creationdate>2021</creationdate><title>Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus</title><author>Calderwood, Alexander ; Lloyd, Andrew ; Hepworth, Jo ; Tudor, Eleri H ; Jones, Marc ; Woodhouse, Shannon ; Bilham, Lorelei ; Chinoy, Catherine ; Williams, Kevin ; Corke, Fiona ; Doonan, John H ; Ostergaard, Lars ; Irwin, Judith A ; Wells, Rachel ; Morris, Richard J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-ghent_librecat_oai_archive_ugent_be_86947683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>ARABIDOPSIS-THALIANA</topic><topic>Biology and Life Sciences</topic><topic>Brassica napus</topic><topic>CABBAGE</topic><topic>DOSAGE Author Information</topic><topic>EVOLUTION</topic><topic>flowering locus C</topic><topic>GENE</topic><topic>gene dosage balance</topic><topic>LOCUS-C</topic><topic>MADS DOMAIN PROTEIN</topic><topic>modelling</topic><topic>NATURAL VARIATION</topic><topic>phenotypic plasticity</topic><topic>polyploidy</topic><topic>TIME</topic><topic>transcriptomics</topic><topic>vernalisation</topic><topic>VERNALIZATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Calderwood, Alexander</creatorcontrib><creatorcontrib>Lloyd, Andrew</creatorcontrib><creatorcontrib>Hepworth, Jo</creatorcontrib><creatorcontrib>Tudor, Eleri H</creatorcontrib><creatorcontrib>Jones, Marc</creatorcontrib><creatorcontrib>Woodhouse, Shannon</creatorcontrib><creatorcontrib>Bilham, Lorelei</creatorcontrib><creatorcontrib>Chinoy, Catherine</creatorcontrib><creatorcontrib>Williams, Kevin</creatorcontrib><creatorcontrib>Corke, Fiona</creatorcontrib><creatorcontrib>Doonan, John H</creatorcontrib><creatorcontrib>Ostergaard, Lars</creatorcontrib><creatorcontrib>Irwin, Judith A</creatorcontrib><creatorcontrib>Wells, Rachel</creatorcontrib><creatorcontrib>Morris, Richard J</creatorcontrib><collection>Ghent University Academic Bibliography</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Calderwood, Alexander</au><au>Lloyd, Andrew</au><au>Hepworth, Jo</au><au>Tudor, Eleri H</au><au>Jones, Marc</au><au>Woodhouse, Shannon</au><au>Bilham, Lorelei</au><au>Chinoy, Catherine</au><au>Williams, Kevin</au><au>Corke, Fiona</au><au>Doonan, John H</au><au>Ostergaard, Lars</au><au>Irwin, Judith A</au><au>Wells, Rachel</au><au>Morris, Richard J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus</atitle><date>2021</date><risdate>2021</risdate><issn>0028-646X</issn><issn>1469-8137</issn><abstract>Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation requirement as a system. We collected transcriptome time series for Brassica napus spring, winter, semi-winter, and Siberian kale crop types. Modelling was used to link FLC expression dynamics to floral response following vernalisation. We show that relaxed selection pressure has allowed expression of FLC paralogues to diverge, resulting in variation of FLC expression during cold treatment between paralogues and accessions. We find that total FLC expression dynamics best explains differences in cold requirement between cultivars, rather than expression of specific FLC paralogues. The combination of multiple FLC paralogues with different expression dynamics leads to rich behaviour in response to cold and a wide range of vernalisation requirements in B. napus. We find evidence for different strategies to determine the response to cold in existing winter rapeseed accessions.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0028-646X
ispartof
issn 0028-646X
1469-8137
language eng
recordid cdi_ghent_librecat_oai_archive_ugent_be_8694768
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Ghent University Academic Bibliography; Wiley Online Library (Open Access Collection)
subjects ARABIDOPSIS-THALIANA
Biology and Life Sciences
Brassica napus
CABBAGE
DOSAGE Author Information
EVOLUTION
flowering locus C
GENE
gene dosage balance
LOCUS-C
MADS DOMAIN PROTEIN
modelling
NATURAL VARIATION
phenotypic plasticity
polyploidy
TIME
transcriptomics
vernalisation
VERNALIZATION
title Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T20%3A41%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-ghent&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Total%20FLC%20transcript%20dynamics%20from%20divergent%20paralogue%20expression%20explains%20flowering%20diversity%20in%20Brassica%20napus&rft.au=Calderwood,%20Alexander&rft.date=2021&rft.issn=0028-646X&rft_id=info:doi/&rft_dat=%3Cghent%3Eoai_archive_ugent_be_8694768%3C/ghent%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true