Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus

Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation req...

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Hauptverfasser: Calderwood, Alexander, Lloyd, Andrew, Hepworth, Jo, Tudor, Eleri H, Jones, Marc, Woodhouse, Shannon, Bilham, Lorelei, Chinoy, Catherine, Williams, Kevin, Corke, Fiona, Doonan, John H, Ostergaard, Lars, Irwin, Judith A, Wells, Rachel, Morris, Richard J
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creator Calderwood, Alexander
Lloyd, Andrew
Hepworth, Jo
Tudor, Eleri H
Jones, Marc
Woodhouse, Shannon
Bilham, Lorelei
Chinoy, Catherine
Williams, Kevin
Corke, Fiona
Doonan, John H
Ostergaard, Lars
Irwin, Judith A
Wells, Rachel
Morris, Richard J
description Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation requirement as a system. We collected transcriptome time series for Brassica napus spring, winter, semi-winter, and Siberian kale crop types. Modelling was used to link FLC expression dynamics to floral response following vernalisation. We show that relaxed selection pressure has allowed expression of FLC paralogues to diverge, resulting in variation of FLC expression during cold treatment between paralogues and accessions. We find that total FLC expression dynamics best explains differences in cold requirement between cultivars, rather than expression of specific FLC paralogues. The combination of multiple FLC paralogues with different expression dynamics leads to rich behaviour in response to cold and a wide range of vernalisation requirements in B. napus. We find evidence for different strategies to determine the response to cold in existing winter rapeseed accessions.
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In Arabidopsis, natural variation in expression levels of the floral repressor FLC leads to differences in vernalisation. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalisation requirement as a system. We collected transcriptome time series for Brassica napus spring, winter, semi-winter, and Siberian kale crop types. Modelling was used to link FLC expression dynamics to floral response following vernalisation. We show that relaxed selection pressure has allowed expression of FLC paralogues to diverge, resulting in variation of FLC expression during cold treatment between paralogues and accessions. We find that total FLC expression dynamics best explains differences in cold requirement between cultivars, rather than expression of specific FLC paralogues. The combination of multiple FLC paralogues with different expression dynamics leads to rich behaviour in response to cold and a wide range of vernalisation requirements in B. napus. 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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Ghent University Academic Bibliography; Wiley Free Content
subjects ARABIDOPSIS-THALIANA
Biology and Life Sciences
Brassica napus
CABBAGE
DOSAGE Author Information
EVOLUTION
flowering locus C
GENE
gene dosage balance
LOCUS-C
MADS DOMAIN PROTEIN
modelling
NATURAL VARIATION
phenotypic plasticity
polyploidy
TIME
transcriptomics
vernalisation
VERNALIZATION
title Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus
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