A chip-based 128-channel potentiostat for high-throughput studies of bioelectrochemical systems : optimal electrode potentials for anodic biofilms

The presence of microorganisms performing extracellular electron transfer has been established in many environments. Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potent...

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Hauptverfasser: Molderez, Tom, Prévoteau, Antonin, Ceyssens, Frederik, Verhelst, Marian, Rabaey, Korneel
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creator Molderez, Tom
Prévoteau, Antonin
Ceyssens, Frederik
Verhelst, Marian
Rabaey, Korneel
description The presence of microorganisms performing extracellular electron transfer has been established in many environments. Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potentiostat, connected to a 128 gold electrode array. Whereas the system is able to perform simultaneously 128 (bio)electrochemical measurements with an independent electrical signal input, the present manufacturing of the array limited the number of effective channels for this study to 77. We assessed the impact of 11 electrode potentials ranging from -0.45 V to +0.2 V vs. Ag/AgCl (7 replicates per potential) on the growth and electrochemical characteristics of anodic electroactive biofilms (EABs) formed by acetate-fed microbial communities. After 7 days of growth, maximum current was reached for electrodes poised at -0.3 V, closely followed by -0.25 V and -0.1 V to +0.1 V, a range well-fitting the midpoint potential of minerals naturally reduced by electroactive bacteria such as Geobacter Sulfurreducens. There was no significant difference in apparent midpoint potential of the EABs (-0.35 V), suggesting that the mechanism of heterogeneous electron transfer was not affected by the electrode potential. The EABs poised below current plateau potential (
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Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potentiostat, connected to a 128 gold electrode array. Whereas the system is able to perform simultaneously 128 (bio)electrochemical measurements with an independent electrical signal input, the present manufacturing of the array limited the number of effective channels for this study to 77. We assessed the impact of 11 electrode potentials ranging from -0.45 V to +0.2 V vs. Ag/AgCl (7 replicates per potential) on the growth and electrochemical characteristics of anodic electroactive biofilms (EABs) formed by acetate-fed microbial communities. After 7 days of growth, maximum current was reached for electrodes poised at -0.3 V, closely followed by -0.25 V and -0.1 V to +0.1 V, a range well-fitting the midpoint potential of minerals naturally reduced by electroactive bacteria such as Geobacter Sulfurreducens. There was no significant difference in apparent midpoint potential of the EABs (-0.35 V), suggesting that the mechanism of heterogeneous electron transfer was not affected by the electrode potential. The EABs poised below current plateau potential (&lt;= -0.3 V) exhibited slower growth but higher charge transfer parameters. The high-throughput and high reproducibility provided by the array may have a major facilitating impact on the field of electromicrobiology. 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source Ghent University Academic Bibliography; Access via ScienceDirect (Elsevier)
subjects Biomedical Engineering
Biophysics
Biotechnology
CHARGE
Charge transport parameter
Chemistry
Electroactive biofilm
ELECTROACTIVE BIOFILMS
Electrochemistry
Electrode array
General Medicine
GENERATION
GEOBACTER-SULFURREDUCENS
High-throughput experiment
MICROBIAL FUEL-CELLS
MICROORGANISMS
Multichannel potentiostat
PERIODIC POLARIZATION
REDUCTION
Technology and Engineering
TRANSPORT
title A chip-based 128-channel potentiostat for high-throughput studies of bioelectrochemical systems : optimal electrode potentials for anodic biofilms
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