A chip-based 128-channel potentiostat for high-throughput studies of bioelectrochemical systems : optimal electrode potentials for anodic biofilms
The presence of microorganisms performing extracellular electron transfer has been established in many environments. Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potent...
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creator | Molderez, Tom Prévoteau, Antonin Ceyssens, Frederik Verhelst, Marian Rabaey, Korneel |
description | The presence of microorganisms performing extracellular electron transfer has been established in many environments. Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potentiostat, connected to a 128 gold electrode array. Whereas the system is able to perform simultaneously 128 (bio)electrochemical measurements with an independent electrical signal input, the present manufacturing of the array limited the number of effective channels for this study to 77. We assessed the impact of 11 electrode potentials ranging from -0.45 V to +0.2 V vs. Ag/AgCl (7 replicates per potential) on the growth and electrochemical characteristics of anodic electroactive biofilms (EABs) formed by acetate-fed microbial communities. After 7 days of growth, maximum current was reached for electrodes poised at -0.3 V, closely followed by -0.25 V and -0.1 V to +0.1 V, a range well-fitting the midpoint potential of minerals naturally reduced by electroactive bacteria such as Geobacter Sulfurreducens. There was no significant difference in apparent midpoint potential of the EABs (-0.35 V), suggesting that the mechanism of heterogeneous electron transfer was not affected by the electrode potential. The EABs poised below current plateau potential ( |
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Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potentiostat, connected to a 128 gold electrode array. Whereas the system is able to perform simultaneously 128 (bio)electrochemical measurements with an independent electrical signal input, the present manufacturing of the array limited the number of effective channels for this study to 77. We assessed the impact of 11 electrode potentials ranging from -0.45 V to +0.2 V vs. Ag/AgCl (7 replicates per potential) on the growth and electrochemical characteristics of anodic electroactive biofilms (EABs) formed by acetate-fed microbial communities. After 7 days of growth, maximum current was reached for electrodes poised at -0.3 V, closely followed by -0.25 V and -0.1 V to +0.1 V, a range well-fitting the midpoint potential of minerals naturally reduced by electroactive bacteria such as Geobacter Sulfurreducens. There was no significant difference in apparent midpoint potential of the EABs (-0.35 V), suggesting that the mechanism of heterogeneous electron transfer was not affected by the electrode potential. The EABs poised below current plateau potential (<= -0.3 V) exhibited slower growth but higher charge transfer parameters. The high-throughput and high reproducibility provided by the array may have a major facilitating impact on the field of electromicrobiology. Key aspects to improve are data processing algorithms to deal with the vast amount of generated data, and manufacturing of the electrode array itself.</description><identifier>ISSN: 1873-4235</identifier><identifier>ISSN: 0956-5663</identifier><language>eng</language><subject>Biomedical Engineering ; Biophysics ; Biotechnology ; CHARGE ; Charge transport parameter ; Chemistry ; Electroactive biofilm ; ELECTROACTIVE BIOFILMS ; Electrochemistry ; Electrode array ; General Medicine ; GENERATION ; GEOBACTER-SULFURREDUCENS ; High-throughput experiment ; MICROBIAL FUEL-CELLS ; MICROORGANISMS ; Multichannel potentiostat ; PERIODIC POLARIZATION ; REDUCTION ; Technology and Engineering ; TRANSPORT</subject><creationdate>2021</creationdate><rights>No license (in copyright) info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,315,780,784,4024,27860</link.rule.ids></links><search><creatorcontrib>Molderez, Tom</creatorcontrib><creatorcontrib>Prévoteau, Antonin</creatorcontrib><creatorcontrib>Ceyssens, Frederik</creatorcontrib><creatorcontrib>Verhelst, Marian</creatorcontrib><creatorcontrib>Rabaey, Korneel</creatorcontrib><title>A chip-based 128-channel potentiostat for high-throughput studies of bioelectrochemical systems : optimal electrode potentials for anodic biofilms</title><description>The presence of microorganisms performing extracellular electron transfer has been established in many environments. Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potentiostat, connected to a 128 gold electrode array. Whereas the system is able to perform simultaneously 128 (bio)electrochemical measurements with an independent electrical signal input, the present manufacturing of the array limited the number of effective channels for this study to 77. We assessed the impact of 11 electrode potentials ranging from -0.45 V to +0.2 V vs. Ag/AgCl (7 replicates per potential) on the growth and electrochemical characteristics of anodic electroactive biofilms (EABs) formed by acetate-fed microbial communities. After 7 days of growth, maximum current was reached for electrodes poised at -0.3 V, closely followed by -0.25 V and -0.1 V to +0.1 V, a range well-fitting the midpoint potential of minerals naturally reduced by electroactive bacteria such as Geobacter Sulfurreducens. There was no significant difference in apparent midpoint potential of the EABs (-0.35 V), suggesting that the mechanism of heterogeneous electron transfer was not affected by the electrode potential. The EABs poised below current plateau potential (<= -0.3 V) exhibited slower growth but higher charge transfer parameters. The high-throughput and high reproducibility provided by the array may have a major facilitating impact on the field of electromicrobiology. Key aspects to improve are data processing algorithms to deal with the vast amount of generated data, and manufacturing of the electrode array itself.</description><subject>Biomedical Engineering</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>CHARGE</subject><subject>Charge transport parameter</subject><subject>Chemistry</subject><subject>Electroactive biofilm</subject><subject>ELECTROACTIVE BIOFILMS</subject><subject>Electrochemistry</subject><subject>Electrode array</subject><subject>General Medicine</subject><subject>GENERATION</subject><subject>GEOBACTER-SULFURREDUCENS</subject><subject>High-throughput experiment</subject><subject>MICROBIAL FUEL-CELLS</subject><subject>MICROORGANISMS</subject><subject>Multichannel potentiostat</subject><subject>PERIODIC POLARIZATION</subject><subject>REDUCTION</subject><subject>Technology and Engineering</subject><subject>TRANSPORT</subject><issn>1873-4235</issn><issn>0956-5663</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ADGLB</sourceid><recordid>eNqdjltKxEAQRftDYcbHHmoDDXk4k4x_IooL8L_pdCrpkk4qdFUEt-GKzci4Ab8u3MfhXpl92Ta1fajqw87ciHwURdGUp2Jvvp8gRFps5wV7KKvWhujnGRMsrDgrsahXGDhDpDFajZnXMS6rgujaEwrwAB0xJgyaOUScKPgE8iWKk8Aj8KI0bc6l0eMf2if5BfuZewpnyEBpkjtzPWwR3l_01lSvL-_Pb3aM28ol6jIGr449OZ-395_o1vEcdejaY3s4NmX9r9EPs-lkdw</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Molderez, Tom</creator><creator>Prévoteau, Antonin</creator><creator>Ceyssens, Frederik</creator><creator>Verhelst, Marian</creator><creator>Rabaey, Korneel</creator><scope>ADGLB</scope></search><sort><creationdate>2021</creationdate><title>A chip-based 128-channel potentiostat for high-throughput studies of bioelectrochemical systems : optimal electrode potentials for anodic biofilms</title><author>Molderez, Tom ; Prévoteau, Antonin ; Ceyssens, Frederik ; Verhelst, Marian ; Rabaey, Korneel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-ghent_librecat_oai_archive_ugent_be_86856713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomedical Engineering</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>CHARGE</topic><topic>Charge transport parameter</topic><topic>Chemistry</topic><topic>Electroactive biofilm</topic><topic>ELECTROACTIVE BIOFILMS</topic><topic>Electrochemistry</topic><topic>Electrode array</topic><topic>General Medicine</topic><topic>GENERATION</topic><topic>GEOBACTER-SULFURREDUCENS</topic><topic>High-throughput experiment</topic><topic>MICROBIAL FUEL-CELLS</topic><topic>MICROORGANISMS</topic><topic>Multichannel potentiostat</topic><topic>PERIODIC POLARIZATION</topic><topic>REDUCTION</topic><topic>Technology and Engineering</topic><topic>TRANSPORT</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Molderez, Tom</creatorcontrib><creatorcontrib>Prévoteau, Antonin</creatorcontrib><creatorcontrib>Ceyssens, Frederik</creatorcontrib><creatorcontrib>Verhelst, Marian</creatorcontrib><creatorcontrib>Rabaey, Korneel</creatorcontrib><collection>Ghent University Academic Bibliography</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Molderez, Tom</au><au>Prévoteau, Antonin</au><au>Ceyssens, Frederik</au><au>Verhelst, Marian</au><au>Rabaey, Korneel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A chip-based 128-channel potentiostat for high-throughput studies of bioelectrochemical systems : optimal electrode potentials for anodic biofilms</atitle><date>2021</date><risdate>2021</risdate><issn>1873-4235</issn><issn>0956-5663</issn><abstract>The presence of microorganisms performing extracellular electron transfer has been established in many environments. Research to determine their role is moving slowly due to the high cost of potentiostats and the variance of data with small number of replicates. Here, we present a 128-channel potentiostat, connected to a 128 gold electrode array. Whereas the system is able to perform simultaneously 128 (bio)electrochemical measurements with an independent electrical signal input, the present manufacturing of the array limited the number of effective channels for this study to 77. We assessed the impact of 11 electrode potentials ranging from -0.45 V to +0.2 V vs. Ag/AgCl (7 replicates per potential) on the growth and electrochemical characteristics of anodic electroactive biofilms (EABs) formed by acetate-fed microbial communities. After 7 days of growth, maximum current was reached for electrodes poised at -0.3 V, closely followed by -0.25 V and -0.1 V to +0.1 V, a range well-fitting the midpoint potential of minerals naturally reduced by electroactive bacteria such as Geobacter Sulfurreducens. There was no significant difference in apparent midpoint potential of the EABs (-0.35 V), suggesting that the mechanism of heterogeneous electron transfer was not affected by the electrode potential. The EABs poised below current plateau potential (<= -0.3 V) exhibited slower growth but higher charge transfer parameters. The high-throughput and high reproducibility provided by the array may have a major facilitating impact on the field of electromicrobiology. Key aspects to improve are data processing algorithms to deal with the vast amount of generated data, and manufacturing of the electrode array itself.</abstract><oa>free_for_read</oa></addata></record> |
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source | Ghent University Academic Bibliography; Access via ScienceDirect (Elsevier) |
subjects | Biomedical Engineering Biophysics Biotechnology CHARGE Charge transport parameter Chemistry Electroactive biofilm ELECTROACTIVE BIOFILMS Electrochemistry Electrode array General Medicine GENERATION GEOBACTER-SULFURREDUCENS High-throughput experiment MICROBIAL FUEL-CELLS MICROORGANISMS Multichannel potentiostat PERIODIC POLARIZATION REDUCTION Technology and Engineering TRANSPORT |
title | A chip-based 128-channel potentiostat for high-throughput studies of bioelectrochemical systems : optimal electrode potentials for anodic biofilms |
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