Development of a rat model for glioma-related epilepsy
Seizures are common in patients with high-grade gliomas (30–60%) and approximately 15–30% of glioblastoma (GB) patients develop drug-resistant epilepsy. Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB c...
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| creator | Bouckaert, Charlotte Germonpré, Charlotte Verhoeven, Jeroen Chong, Seon-Ah Jacquin, Lucas Mairet-Coello, Georges André, Véronique Marie Leclercq, Karine Vanhove, Christian De Vos, Filip Van den Broecke, Caroline Goethals, Ingeborg Descamps, Benedicte Donche, Sam Carrette, Evelien Wadman, Wytse Boon, Paul Vonck, Kristl Raedt, Robrecht |
| description | Seizures are common in patients with high-grade gliomas (30–60%) and approximately 15–30% of glioblastoma (GB) patients develop drug-resistant epilepsy. Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain. This study describes, for the first time, reproducible tumor growth and spontaneous seizures upon inoculation of F98 cells in the rat entorhinal cortex. The development of this new model of GB-related epilepsy may be valuable to design new therapies against tumor growth and associated epileptic seizures. |
| format | Article |
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Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain. This study describes, for the first time, reproducible tumor growth and spontaneous seizures upon inoculation of F98 cells in the rat entorhinal cortex. The development of this new model of GB-related epilepsy may be valuable to design new therapies against tumor growth and associated epileptic seizures.</description><identifier>ISSN: 1422-0067</identifier><language>eng</language><subject>BRAIN-TUMOR ; Catalysis ; Computer Science Applications ; General Medicine ; glioma ; glioma-related epilepsy ; GLUTAMATE ; high-grade glioma rat model ; Inorganic Chemistry ; MANAGEMENT ; Medicine and Health Sciences ; Molecular Biology ; Organic Chemistry ; PATHOPHYSIOLOGY ; Physical and Theoretical Chemistry ; seizures ; Spectroscopy ; TUMOR-ASSOCIATED EPILEPSY ; video-EEG monitoring</subject><creationdate>2020</creationdate><rights>Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,316,782,786,4028,27869</link.rule.ids></links><search><creatorcontrib>Bouckaert, Charlotte</creatorcontrib><creatorcontrib>Germonpré, Charlotte</creatorcontrib><creatorcontrib>Verhoeven, Jeroen</creatorcontrib><creatorcontrib>Chong, Seon-Ah</creatorcontrib><creatorcontrib>Jacquin, Lucas</creatorcontrib><creatorcontrib>Mairet-Coello, Georges</creatorcontrib><creatorcontrib>André, Véronique Marie</creatorcontrib><creatorcontrib>Leclercq, Karine</creatorcontrib><creatorcontrib>Vanhove, Christian</creatorcontrib><creatorcontrib>De Vos, Filip</creatorcontrib><creatorcontrib>Van den Broecke, Caroline</creatorcontrib><creatorcontrib>Goethals, Ingeborg</creatorcontrib><creatorcontrib>Descamps, Benedicte</creatorcontrib><creatorcontrib>Donche, Sam</creatorcontrib><creatorcontrib>Carrette, Evelien</creatorcontrib><creatorcontrib>Wadman, Wytse</creatorcontrib><creatorcontrib>Boon, Paul</creatorcontrib><creatorcontrib>Vonck, Kristl</creatorcontrib><creatorcontrib>Raedt, Robrecht</creatorcontrib><title>Development of a rat model for glioma-related epilepsy</title><description>Seizures are common in patients with high-grade gliomas (30–60%) and approximately 15–30% of glioblastoma (GB) patients develop drug-resistant epilepsy. Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain. This study describes, for the first time, reproducible tumor growth and spontaneous seizures upon inoculation of F98 cells in the rat entorhinal cortex. The development of this new model of GB-related epilepsy may be valuable to design new therapies against tumor growth and associated epileptic seizures.</description><subject>BRAIN-TUMOR</subject><subject>Catalysis</subject><subject>Computer Science Applications</subject><subject>General Medicine</subject><subject>glioma</subject><subject>glioma-related epilepsy</subject><subject>GLUTAMATE</subject><subject>high-grade glioma rat model</subject><subject>Inorganic Chemistry</subject><subject>MANAGEMENT</subject><subject>Medicine and Health Sciences</subject><subject>Molecular Biology</subject><subject>Organic Chemistry</subject><subject>PATHOPHYSIOLOGY</subject><subject>Physical and Theoretical Chemistry</subject><subject>seizures</subject><subject>Spectroscopy</subject><subject>TUMOR-ASSOCIATED EPILEPSY</subject><subject>video-EEG monitoring</subject><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ADGLB</sourceid><recordid>eNqdjEsKwjAUALNQsH7u8C5QiKlJde0HD-A-vLavbeTVlCQWvL0KnsDVLIaZmci2O6VyKU25EMsY71KqQulDJsyJJmI_DvRI4FtACJhg8A0xtD5Ax84PmAdiTNQAjY5pjK-1mLfIkTY_roS6nG_Ha971n5FlVwWqMVmPzmKoezeRfXZfVZHdm1JrZYq_oje4LkFs</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Bouckaert, Charlotte</creator><creator>Germonpré, Charlotte</creator><creator>Verhoeven, Jeroen</creator><creator>Chong, Seon-Ah</creator><creator>Jacquin, Lucas</creator><creator>Mairet-Coello, Georges</creator><creator>André, Véronique Marie</creator><creator>Leclercq, Karine</creator><creator>Vanhove, Christian</creator><creator>De Vos, Filip</creator><creator>Van den Broecke, Caroline</creator><creator>Goethals, Ingeborg</creator><creator>Descamps, Benedicte</creator><creator>Donche, Sam</creator><creator>Carrette, Evelien</creator><creator>Wadman, Wytse</creator><creator>Boon, Paul</creator><creator>Vonck, Kristl</creator><creator>Raedt, Robrecht</creator><scope>ADGLB</scope></search><sort><creationdate>2020</creationdate><title>Development of a rat model for glioma-related epilepsy</title><author>Bouckaert, Charlotte ; 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Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain. This study describes, for the first time, reproducible tumor growth and spontaneous seizures upon inoculation of F98 cells in the rat entorhinal cortex. The development of this new model of GB-related epilepsy may be valuable to design new therapies against tumor growth and associated epileptic seizures.</abstract><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; Ghent University Academic Bibliography; PubMed Central |
| subjects | BRAIN-TUMOR Catalysis Computer Science Applications General Medicine glioma glioma-related epilepsy GLUTAMATE high-grade glioma rat model Inorganic Chemistry MANAGEMENT Medicine and Health Sciences Molecular Biology Organic Chemistry PATHOPHYSIOLOGY Physical and Theoretical Chemistry seizures Spectroscopy TUMOR-ASSOCIATED EPILEPSY video-EEG monitoring |
| title | Development of a rat model for glioma-related epilepsy |
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