Identification of the Brassinazole-Resistant and the Molecular Cloning and Functional Characterization of TaBZR2.1
Brassinazole-resistant (BZR) transcription factors are important transcription factors in Brassinosteroid (BR)-responsive gene expression. However, limited knowledge exists regarding the BZR genes in wheat and a limited number of BZR family genes have been previously reported in wheat. In this study...
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| Veröffentlicht in: | International journal of molecular sciences 2024-12, Vol.25 (23) |
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| container_title | International journal of molecular sciences |
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| creator | Zhang, Yan Qin, Jingzi Hou, Jinna Liu, Congcong Geng, Shenghui Qin, Maomao Li, Wenxu Dai, Ziju Wu, Zhengqing Lei, Zhensheng Zhou, Zhengfu |
| description | Brassinazole-resistant (BZR) transcription factors are important transcription factors in Brassinosteroid (BR)-responsive gene expression. However, limited knowledge exists regarding the BZR genes in wheat and a limited number of BZR family genes have been previously reported in wheat. In this study, the synteny analyses of the TaBZR genes suggested that gene duplication events have played an essential role in the TaBZR family during evolution. The results of RT-qPCR and transcriptome data analyses exhibited remarkable expression patterns in the BZR genes in different tissues and under different treatments. The yeast two-hybrid (Y2H) screen result showed that the TaBZR2.1 protein interacts with Argonaute 4 (AGO4). Taken together, our results not only provide us a basis for understanding the molecular characteristics and expression patterns of the TaBZR family genes but also offered the functional characterization of TaBZR2.1 in wheat. |
| doi_str_mv | 10.3390/ijms252312545 |
| format | Article |
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However, limited knowledge exists regarding the BZR genes in wheat and a limited number of BZR family genes have been previously reported in wheat. In this study, the synteny analyses of the TaBZR genes suggested that gene duplication events have played an essential role in the TaBZR family during evolution. The results of RT-qPCR and transcriptome data analyses exhibited remarkable expression patterns in the BZR genes in different tissues and under different treatments. The yeast two-hybrid (Y2H) screen result showed that the TaBZR2.1 protein interacts with Argonaute 4 (AGO4). Taken together, our results not only provide us a basis for understanding the molecular characteristics and expression patterns of the TaBZR family genes but also offered the functional characterization of TaBZR2.1 in wheat.</description><identifier>ISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms252312545</identifier><language>eng</language><publisher>MDPI AG</publisher><subject>Cloning ; DNA binding proteins ; Genes ; Genetic transcription ; Information management ; Wheat</subject><ispartof>International journal of molecular sciences, 2024-12, Vol.25 (23)</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Qin, Jingzi</creatorcontrib><creatorcontrib>Hou, Jinna</creatorcontrib><creatorcontrib>Liu, Congcong</creatorcontrib><creatorcontrib>Geng, Shenghui</creatorcontrib><creatorcontrib>Qin, Maomao</creatorcontrib><creatorcontrib>Li, Wenxu</creatorcontrib><creatorcontrib>Dai, Ziju</creatorcontrib><creatorcontrib>Wu, Zhengqing</creatorcontrib><creatorcontrib>Lei, Zhensheng</creatorcontrib><creatorcontrib>Zhou, Zhengfu</creatorcontrib><title>Identification of the Brassinazole-Resistant and the Molecular Cloning and Functional Characterization of TaBZR2.1</title><title>International journal of molecular sciences</title><description>Brassinazole-resistant (BZR) transcription factors are important transcription factors in Brassinosteroid (BR)-responsive gene expression. However, limited knowledge exists regarding the BZR genes in wheat and a limited number of BZR family genes have been previously reported in wheat. In this study, the synteny analyses of the TaBZR genes suggested that gene duplication events have played an essential role in the TaBZR family during evolution. The results of RT-qPCR and transcriptome data analyses exhibited remarkable expression patterns in the BZR genes in different tissues and under different treatments. The yeast two-hybrid (Y2H) screen result showed that the TaBZR2.1 protein interacts with Argonaute 4 (AGO4). Taken together, our results not only provide us a basis for understanding the molecular characteristics and expression patterns of the TaBZR family genes but also offered the functional characterization of TaBZR2.1 in wheat.</description><subject>Cloning</subject><subject>DNA binding proteins</subject><subject>Genes</subject><subject>Genetic transcription</subject><subject>Information management</subject><subject>Wheat</subject><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptjk1Lw0AQhvegYK0evQc8J87sZpvssQ1WCxWh9OSlTPej3ZJuILu99Ncbq4gHeQ8D78fDMPaAUAih4MkfjpFLLpDLUl6xEZac5wCT6obdxngA4IJLNWL9wtiQvPOaku9C1rks7W026ylGH-jctTZf2ehjopAyCuYSvw22PrXUZ03bBR92l2R-CvoLQm3W7KknnWzvz7_cNc0-VrzAO3btqI32_ueO2Xr-vG5e8-X7y6KZLvPdpJK5kQ4cCLXdKqF1VSKSRGd1OZHgLNQkCJSTBqptbSyidqZ0qsZaq1KhrcWYPX5jd9TajQ-uS8NLRx_1ZlqjUhIFyKFV_NMaZOzR6y5Y5wf_z-ATVqFq8g</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Zhang, Yan</creator><creator>Qin, Jingzi</creator><creator>Hou, Jinna</creator><creator>Liu, Congcong</creator><creator>Geng, Shenghui</creator><creator>Qin, Maomao</creator><creator>Li, Wenxu</creator><creator>Dai, Ziju</creator><creator>Wu, Zhengqing</creator><creator>Lei, Zhensheng</creator><creator>Zhou, Zhengfu</creator><general>MDPI AG</general><scope/></search><sort><creationdate>20241201</creationdate><title>Identification of the Brassinazole-Resistant and the Molecular Cloning and Functional Characterization of TaBZR2.1</title><author>Zhang, Yan ; Qin, Jingzi ; Hou, Jinna ; Liu, Congcong ; Geng, Shenghui ; Qin, Maomao ; Li, Wenxu ; Dai, Ziju ; Wu, Zhengqing ; Lei, Zhensheng ; Zhou, Zhengfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g675-d5f0f039bb93cc7411a51fec4650fe08a3a09f5d07b8de11cfd4f9818c9491e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cloning</topic><topic>DNA binding proteins</topic><topic>Genes</topic><topic>Genetic transcription</topic><topic>Information management</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Qin, Jingzi</creatorcontrib><creatorcontrib>Hou, Jinna</creatorcontrib><creatorcontrib>Liu, Congcong</creatorcontrib><creatorcontrib>Geng, Shenghui</creatorcontrib><creatorcontrib>Qin, Maomao</creatorcontrib><creatorcontrib>Li, Wenxu</creatorcontrib><creatorcontrib>Dai, Ziju</creatorcontrib><creatorcontrib>Wu, Zhengqing</creatorcontrib><creatorcontrib>Lei, Zhensheng</creatorcontrib><creatorcontrib>Zhou, Zhengfu</creatorcontrib><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yan</au><au>Qin, Jingzi</au><au>Hou, Jinna</au><au>Liu, Congcong</au><au>Geng, Shenghui</au><au>Qin, Maomao</au><au>Li, Wenxu</au><au>Dai, Ziju</au><au>Wu, Zhengqing</au><au>Lei, Zhensheng</au><au>Zhou, Zhengfu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of the Brassinazole-Resistant and the Molecular Cloning and Functional Characterization of TaBZR2.1</atitle><jtitle>International journal of molecular sciences</jtitle><date>2024-12-01</date><risdate>2024</risdate><volume>25</volume><issue>23</issue><issn>1422-0067</issn><abstract>Brassinazole-resistant (BZR) transcription factors are important transcription factors in Brassinosteroid (BR)-responsive gene expression. However, limited knowledge exists regarding the BZR genes in wheat and a limited number of BZR family genes have been previously reported in wheat. In this study, the synteny analyses of the TaBZR genes suggested that gene duplication events have played an essential role in the TaBZR family during evolution. The results of RT-qPCR and transcriptome data analyses exhibited remarkable expression patterns in the BZR genes in different tissues and under different treatments. The yeast two-hybrid (Y2H) screen result showed that the TaBZR2.1 protein interacts with Argonaute 4 (AGO4). Taken together, our results not only provide us a basis for understanding the molecular characteristics and expression patterns of the TaBZR family genes but also offered the functional characterization of TaBZR2.1 in wheat.</abstract><pub>MDPI AG</pub><doi>10.3390/ijms252312545</doi></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cloning DNA binding proteins Genes Genetic transcription Information management Wheat |
| title | Identification of the Brassinazole-Resistant and the Molecular Cloning and Functional Characterization of TaBZR2.1 |
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