Evaluation of Wound Healing Activity of Hydromethanolic Crude Extract and Solvent Fractions of the Root of Verbascum Sinaiticum Benth
Background: The roots of Verbascum sinaiticum have been used traditionally for the management of wound in different regions of Ethiopia. Despite the presence of several claims and in vitro studies regarding its role in wound healing, no scientific studies have been conducted so far. Therefore, this...
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| Veröffentlicht in: | Journal of experimental pharmacology 2024-04, Vol.16, p.143 |
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| creator | Essa, Aziza M Getahun, Kefyalew A Birhanu, Zewdu B |
| description | Background: The roots of Verbascum sinaiticum have been used traditionally for the management of wound in different regions of Ethiopia. Despite the presence of several claims and in vitro studies regarding its role in wound healing, no scientific studies have been conducted so far. Therefore, this study aims to scientifically evaluate the wound healing activities of the crude extract and solvent fractions of the roots of Verbascum sinaiticum in Swiss albino mice. Methods: The dried root powder of Verbascum sinaiticum was extracted using 80% methanol by maceration technique. This was then fractionated with chloroform, ethyl acetate, and water. These extracts were formulated as ointment at 5% and 10% concentration by using simple base. Acute dermal toxicity was performed on mice. The wound healing potential was evaluated using excision, incision, and burn wound models. Results: In excision wound, 10% and 5% of crude extract ointment provided a significant (P |
| doi_str_mv | 10.2147/JEP.S454096 |
| format | Article |
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Despite the presence of several claims and in vitro studies regarding its role in wound healing, no scientific studies have been conducted so far. Therefore, this study aims to scientifically evaluate the wound healing activities of the crude extract and solvent fractions of the roots of Verbascum sinaiticum in Swiss albino mice. Methods: The dried root powder of Verbascum sinaiticum was extracted using 80% methanol by maceration technique. This was then fractionated with chloroform, ethyl acetate, and water. These extracts were formulated as ointment at 5% and 10% concentration by using simple base. Acute dermal toxicity was performed on mice. The wound healing potential was evaluated using excision, incision, and burn wound models. Results: In excision wound, 10% and 5% of crude extract ointment provided a significant (P<0.001) percentage of contraction starting from day 4 and day 6 onwards respectively. Moreover, the rate of epithelialization was significantly (P<0.001) improved in 10% crude extract. In burn wound, 10% and 5% crude extract showed significant (P<0.001) wound contraction starting from day 4 and 8 onwards respectively. In both excision and burn wounds, a moderate concentration of fibroblast proliferation and collagen deposition was observed on the 10% crude extract. The 5% and 10% aqueous and ethyl acetate fractions produced a significant (P<0.001) percentage of wound contraction and shortening of epithelialization at different time points compared to simple ointment. Conclusion: The results of this study demonstrated that the 80% methanolic crude extract, aqueous and ethyl acetate fractions of Verbascum sinaiticum root have wound healing potential which assimilates its traditional use. Keywords: wound healing, crude extract, solvent fraction, Verbascum sinaiticum</description><identifier>ISSN: 1179-1454</identifier><identifier>EISSN: 1179-1454</identifier><identifier>DOI: 10.2147/JEP.S454096</identifier><language>eng</language><publisher>Dove Medical Press Limited</publisher><subject>Acetates ; Analysis ; Burns and scalds ; Care and treatment ; Esters ; Methanol ; Wound healing ; Wounds and injuries</subject><ispartof>Journal of experimental pharmacology, 2024-04, Vol.16, p.143</ispartof><rights>COPYRIGHT 2024 Dove Medical Press Limited</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Essa, Aziza M</creatorcontrib><creatorcontrib>Getahun, Kefyalew A</creatorcontrib><creatorcontrib>Birhanu, Zewdu B</creatorcontrib><title>Evaluation of Wound Healing Activity of Hydromethanolic Crude Extract and Solvent Fractions of the Root of Verbascum Sinaiticum Benth</title><title>Journal of experimental pharmacology</title><description>Background: The roots of Verbascum sinaiticum have been used traditionally for the management of wound in different regions of Ethiopia. Despite the presence of several claims and in vitro studies regarding its role in wound healing, no scientific studies have been conducted so far. Therefore, this study aims to scientifically evaluate the wound healing activities of the crude extract and solvent fractions of the roots of Verbascum sinaiticum in Swiss albino mice. Methods: The dried root powder of Verbascum sinaiticum was extracted using 80% methanol by maceration technique. This was then fractionated with chloroform, ethyl acetate, and water. These extracts were formulated as ointment at 5% and 10% concentration by using simple base. Acute dermal toxicity was performed on mice. The wound healing potential was evaluated using excision, incision, and burn wound models. Results: In excision wound, 10% and 5% of crude extract ointment provided a significant (P<0.001) percentage of contraction starting from day 4 and day 6 onwards respectively. Moreover, the rate of epithelialization was significantly (P<0.001) improved in 10% crude extract. In burn wound, 10% and 5% crude extract showed significant (P<0.001) wound contraction starting from day 4 and 8 onwards respectively. In both excision and burn wounds, a moderate concentration of fibroblast proliferation and collagen deposition was observed on the 10% crude extract. The 5% and 10% aqueous and ethyl acetate fractions produced a significant (P<0.001) percentage of wound contraction and shortening of epithelialization at different time points compared to simple ointment. Conclusion: The results of this study demonstrated that the 80% methanolic crude extract, aqueous and ethyl acetate fractions of Verbascum sinaiticum root have wound healing potential which assimilates its traditional use. Keywords: wound healing, crude extract, solvent fraction, Verbascum sinaiticum</description><subject>Acetates</subject><subject>Analysis</subject><subject>Burns and scalds</subject><subject>Care and treatment</subject><subject>Esters</subject><subject>Methanol</subject><subject>Wound healing</subject><subject>Wounds and injuries</subject><issn>1179-1454</issn><issn>1179-1454</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqNkV1LwzAUhoMoOOau_AMBwbvOpk2b5nKO6pSB4vy4HGl6skbaBtp0uB_g_zb1AzcQZnKRNyfv856QIHRK_HFAKLu4Te_HCxpRn8cHaEAI4x5x28MtfYxGbfvquxFyziI6QO_pWpSdsNrU2Cj8Yro6xzMQpa5XeCKtXmu76U9mm7wxFdhC1KbUEk-bLgecvtlGSIuFoxamXENt8VVfcXltj9kC8IMxttfP0GSilV2FF7oW2upeXjqkOEFHSpQtjL7XIXq6Sh-nM29-d30zncy9FeFx6EmeM5UoquIsCViWJxSkCCBPQDIKcQJBEtIIgGchKJ8okD5juSIyokEEgQyH6OwrdyVKWOpamf76lW7lcsJ4yKMkIeEeFwk480nsXOM_XG7mUGlpalDa1Xdi_wn8djjfAgr3KbZoTdl9Pu5u8h7jT-IHuLmp9Q</recordid><startdate>20240430</startdate><enddate>20240430</enddate><creator>Essa, Aziza M</creator><creator>Getahun, Kefyalew A</creator><creator>Birhanu, Zewdu B</creator><general>Dove Medical Press Limited</general><scope/></search><sort><creationdate>20240430</creationdate><title>Evaluation of Wound Healing Activity of Hydromethanolic Crude Extract and Solvent Fractions of the Root of Verbascum Sinaiticum Benth</title><author>Essa, Aziza M ; Getahun, Kefyalew A ; Birhanu, Zewdu B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1963-c9d7f8f4f6b827bd84eca2ed8ec74e68e28345ee9b3ef01fec077df1c5425e2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetates</topic><topic>Analysis</topic><topic>Burns and scalds</topic><topic>Care and treatment</topic><topic>Esters</topic><topic>Methanol</topic><topic>Wound healing</topic><topic>Wounds and injuries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Essa, Aziza M</creatorcontrib><creatorcontrib>Getahun, Kefyalew A</creatorcontrib><creatorcontrib>Birhanu, Zewdu B</creatorcontrib><jtitle>Journal of experimental pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Essa, Aziza M</au><au>Getahun, Kefyalew A</au><au>Birhanu, Zewdu B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Wound Healing Activity of Hydromethanolic Crude Extract and Solvent Fractions of the Root of Verbascum Sinaiticum Benth</atitle><jtitle>Journal of experimental pharmacology</jtitle><date>2024-04-30</date><risdate>2024</risdate><volume>16</volume><spage>143</spage><pages>143-</pages><issn>1179-1454</issn><eissn>1179-1454</eissn><abstract>Background: The roots of Verbascum sinaiticum have been used traditionally for the management of wound in different regions of Ethiopia. Despite the presence of several claims and in vitro studies regarding its role in wound healing, no scientific studies have been conducted so far. Therefore, this study aims to scientifically evaluate the wound healing activities of the crude extract and solvent fractions of the roots of Verbascum sinaiticum in Swiss albino mice. Methods: The dried root powder of Verbascum sinaiticum was extracted using 80% methanol by maceration technique. This was then fractionated with chloroform, ethyl acetate, and water. These extracts were formulated as ointment at 5% and 10% concentration by using simple base. Acute dermal toxicity was performed on mice. The wound healing potential was evaluated using excision, incision, and burn wound models. Results: In excision wound, 10% and 5% of crude extract ointment provided a significant (P<0.001) percentage of contraction starting from day 4 and day 6 onwards respectively. Moreover, the rate of epithelialization was significantly (P<0.001) improved in 10% crude extract. In burn wound, 10% and 5% crude extract showed significant (P<0.001) wound contraction starting from day 4 and 8 onwards respectively. In both excision and burn wounds, a moderate concentration of fibroblast proliferation and collagen deposition was observed on the 10% crude extract. The 5% and 10% aqueous and ethyl acetate fractions produced a significant (P<0.001) percentage of wound contraction and shortening of epithelialization at different time points compared to simple ointment. Conclusion: The results of this study demonstrated that the 80% methanolic crude extract, aqueous and ethyl acetate fractions of Verbascum sinaiticum root have wound healing potential which assimilates its traditional use. Keywords: wound healing, crude extract, solvent fraction, Verbascum sinaiticum</abstract><pub>Dove Medical Press Limited</pub><doi>10.2147/JEP.S454096</doi></addata></record> |
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| source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; Dove Press Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Acetates Analysis Burns and scalds Care and treatment Esters Methanol Wound healing Wounds and injuries |
| title | Evaluation of Wound Healing Activity of Hydromethanolic Crude Extract and Solvent Fractions of the Root of Verbascum Sinaiticum Benth |
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