Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru
Heliotropium arborescens (Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions...
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| Veröffentlicht in: | Farmacija 2024-05, Vol.71 (2), p.1-12 |
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| creator | Alvarado, Angel T Chávez, Haydee García, Jorge A Bendezú, María R Surco-Laos, Felipe Molina-Cabrera, Aura Laos-Anchante, Doris Vega-Ramos, Nelly Palomino-Jhong, Juan J Yarasca-Carlos, Paulina Eliades Loja-Herrera, Berta Pineda-Pérez, Mario Bolarte-Arteaga, Mario |
| description | Heliotropium arborescens
(Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the
in vitro
antioxidant and antibacterial activity of the ethanolic extract of
Heliotropium arborescens
L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three
in vitro
methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC
50
37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R
2
0.9989), TFC/ABTS (r = 0.9572; R
2
0.9998) and TFC/FRAP (r = 09933; R
2
0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on
Bacillus subtilis
(ATCC 6633) and
Staphylococcus aureus
(ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of
H. arborescens
L. show potential antioxidant activity, and moderate antibacterial activity against
Bacillus subtilis
and
Staphylococcus aureus
.
Graphical abstract
: |
| doi_str_mv | 10.3897/pharmacia.71.e120315 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_gale_infotracmisc_A793710674</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A793710674</galeid><doaj_id>oai_doaj_org_article_c65ede59452c43aa87932e3994dd8d0d</doaj_id><sourcerecordid>A793710674</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-6b73835309e09d9a1ce92661276139f00443320f323b2c7fc74921217a2983a93</originalsourceid><addsrcrecordid>eNptks9q3DAQxk1poUuaN-hB0LNd_bEt67gNbRNYaA4p9CZmpfFawbaMpE2zL9jnqrwbAoWiw2hGv--bQUxRfGS0Ep2Sn5cBwgTGQSVZhYxTwZo3xYa3VJRNI3-9LTa05l1JuWrfF9cxPlJKeUc7xbtN8eeL88uAsx-dIcZPiz_ONhKYLZkwwUicn3Mao88dElry26WBpAEzkpx_djbHM77mezAJg8uyfHFPLp2I7880pgEuTfA5hfy6Ptzi6HwKfnHHiUDY-4DRYO63q8iI8ISR9MFPZ4PtbBFmEvCQJ1rF2xOYoxl8eY_h-KF418MY8folXhU_v319uLktdz--391sd6URUqWy3UvRiUZQhVRZBcyg4m3LuGyZUD2ldS0Ep73gYs-N7I2sFWecSeCqE6DEVXF38bUeHvUS3AThpD04fS74cNAQkjMjatM2aLFRdcNNLQA6qQRHoVRtbWepzV6fLl4HyLibe79-zOSi0dvMSkZbWWeq-g-Vj8XJGT9j73L9H0F9EZjgYwzYv47JqF43Rr9ujJZMv2yM-AvQo7kj</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Alvarado, Angel T ; Chávez, Haydee ; García, Jorge A ; Bendezú, María R ; Surco-Laos, Felipe ; Molina-Cabrera, Aura ; Laos-Anchante, Doris ; Vega-Ramos, Nelly ; Palomino-Jhong, Juan J ; Yarasca-Carlos, Paulina Eliades ; Loja-Herrera, Berta ; Pineda-Pérez, Mario ; Bolarte-Arteaga, Mario</creator><creatorcontrib>Alvarado, Angel T ; Chávez, Haydee ; García, Jorge A ; Bendezú, María R ; Surco-Laos, Felipe ; Molina-Cabrera, Aura ; Laos-Anchante, Doris ; Vega-Ramos, Nelly ; Palomino-Jhong, Juan J ; Yarasca-Carlos, Paulina Eliades ; Loja-Herrera, Berta ; Pineda-Pérez, Mario ; Bolarte-Arteaga, Mario</creatorcontrib><description>Heliotropium arborescens
(Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the
in vitro
antioxidant and antibacterial activity of the ethanolic extract of
Heliotropium arborescens
L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three
in vitro
methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC
50
37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R
2
0.9989), TFC/ABTS (r = 0.9572; R
2
0.9998) and TFC/FRAP (r = 09933; R
2
0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on
Bacillus subtilis
(ATCC 6633) and
Staphylococcus aureus
(ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of
H. arborescens
L. show potential antioxidant activity, and moderate antibacterial activity against
Bacillus subtilis
and
Staphylococcus aureus
.
Graphical abstract
:</description><identifier>ISSN: 0428-0296</identifier><identifier>EISSN: 2603-557X</identifier><identifier>DOI: 10.3897/pharmacia.71.e120315</identifier><language>eng</language><publisher>Pensoft Publishers</publisher><subject>Antibacterial agents ; Antioxidants ; Isoflavones</subject><ispartof>Farmacija, 2024-05, Vol.71 (2), p.1-12</ispartof><rights>COPYRIGHT 2024 Pensoft Publishers</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c379t-6b73835309e09d9a1ce92661276139f00443320f323b2c7fc74921217a2983a93</cites><orcidid>0000-0001-8694-8924 ; 0000-0002-3053-3057 ; 0000-0002-2454-7081 ; 0000-0003-0805-5535 ; 0000-0002-8717-4307 ; 0000-0001-9880-7344 ; 0000-0001-6818-7797 ; 0000-0001-6560-0486</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>Alvarado, Angel T</creatorcontrib><creatorcontrib>Chávez, Haydee</creatorcontrib><creatorcontrib>García, Jorge A</creatorcontrib><creatorcontrib>Bendezú, María R</creatorcontrib><creatorcontrib>Surco-Laos, Felipe</creatorcontrib><creatorcontrib>Molina-Cabrera, Aura</creatorcontrib><creatorcontrib>Laos-Anchante, Doris</creatorcontrib><creatorcontrib>Vega-Ramos, Nelly</creatorcontrib><creatorcontrib>Palomino-Jhong, Juan J</creatorcontrib><creatorcontrib>Yarasca-Carlos, Paulina Eliades</creatorcontrib><creatorcontrib>Loja-Herrera, Berta</creatorcontrib><creatorcontrib>Pineda-Pérez, Mario</creatorcontrib><creatorcontrib>Bolarte-Arteaga, Mario</creatorcontrib><title>Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru</title><title>Farmacija</title><description>Heliotropium arborescens
(Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the
in vitro
antioxidant and antibacterial activity of the ethanolic extract of
Heliotropium arborescens
L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three
in vitro
methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC
50
37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R
2
0.9989), TFC/ABTS (r = 0.9572; R
2
0.9998) and TFC/FRAP (r = 09933; R
2
0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on
Bacillus subtilis
(ATCC 6633) and
Staphylococcus aureus
(ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of
H. arborescens
L. show potential antioxidant activity, and moderate antibacterial activity against
Bacillus subtilis
and
Staphylococcus aureus
.
Graphical abstract
:</description><subject>Antibacterial agents</subject><subject>Antioxidants</subject><subject>Isoflavones</subject><issn>0428-0296</issn><issn>2603-557X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNptks9q3DAQxk1poUuaN-hB0LNd_bEt67gNbRNYaA4p9CZmpfFawbaMpE2zL9jnqrwbAoWiw2hGv--bQUxRfGS0Ep2Sn5cBwgTGQSVZhYxTwZo3xYa3VJRNI3-9LTa05l1JuWrfF9cxPlJKeUc7xbtN8eeL88uAsx-dIcZPiz_ONhKYLZkwwUicn3Mao88dElry26WBpAEzkpx_djbHM77mezAJg8uyfHFPLp2I7880pgEuTfA5hfy6Ptzi6HwKfnHHiUDY-4DRYO63q8iI8ISR9MFPZ4PtbBFmEvCQJ1rF2xOYoxl8eY_h-KF418MY8folXhU_v319uLktdz--391sd6URUqWy3UvRiUZQhVRZBcyg4m3LuGyZUD2ldS0Ep73gYs-N7I2sFWecSeCqE6DEVXF38bUeHvUS3AThpD04fS74cNAQkjMjatM2aLFRdcNNLQA6qQRHoVRtbWepzV6fLl4HyLibe79-zOSi0dvMSkZbWWeq-g-Vj8XJGT9j73L9H0F9EZjgYwzYv47JqF43Rr9ujJZMv2yM-AvQo7kj</recordid><startdate>20240514</startdate><enddate>20240514</enddate><creator>Alvarado, Angel T</creator><creator>Chávez, Haydee</creator><creator>García, Jorge A</creator><creator>Bendezú, María R</creator><creator>Surco-Laos, Felipe</creator><creator>Molina-Cabrera, Aura</creator><creator>Laos-Anchante, Doris</creator><creator>Vega-Ramos, Nelly</creator><creator>Palomino-Jhong, Juan J</creator><creator>Yarasca-Carlos, Paulina Eliades</creator><creator>Loja-Herrera, Berta</creator><creator>Pineda-Pérez, Mario</creator><creator>Bolarte-Arteaga, Mario</creator><general>Pensoft Publishers</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8694-8924</orcidid><orcidid>https://orcid.org/0000-0002-3053-3057</orcidid><orcidid>https://orcid.org/0000-0002-2454-7081</orcidid><orcidid>https://orcid.org/0000-0003-0805-5535</orcidid><orcidid>https://orcid.org/0000-0002-8717-4307</orcidid><orcidid>https://orcid.org/0000-0001-9880-7344</orcidid><orcidid>https://orcid.org/0000-0001-6818-7797</orcidid><orcidid>https://orcid.org/0000-0001-6560-0486</orcidid></search><sort><creationdate>20240514</creationdate><title>Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru</title><author>Alvarado, Angel T ; Chávez, Haydee ; García, Jorge A ; Bendezú, María R ; Surco-Laos, Felipe ; Molina-Cabrera, Aura ; Laos-Anchante, Doris ; Vega-Ramos, Nelly ; Palomino-Jhong, Juan J ; Yarasca-Carlos, Paulina Eliades ; Loja-Herrera, Berta ; Pineda-Pérez, Mario ; Bolarte-Arteaga, Mario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-6b73835309e09d9a1ce92661276139f00443320f323b2c7fc74921217a2983a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibacterial agents</topic><topic>Antioxidants</topic><topic>Isoflavones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alvarado, Angel T</creatorcontrib><creatorcontrib>Chávez, Haydee</creatorcontrib><creatorcontrib>García, Jorge A</creatorcontrib><creatorcontrib>Bendezú, María R</creatorcontrib><creatorcontrib>Surco-Laos, Felipe</creatorcontrib><creatorcontrib>Molina-Cabrera, Aura</creatorcontrib><creatorcontrib>Laos-Anchante, Doris</creatorcontrib><creatorcontrib>Vega-Ramos, Nelly</creatorcontrib><creatorcontrib>Palomino-Jhong, Juan J</creatorcontrib><creatorcontrib>Yarasca-Carlos, Paulina Eliades</creatorcontrib><creatorcontrib>Loja-Herrera, Berta</creatorcontrib><creatorcontrib>Pineda-Pérez, Mario</creatorcontrib><creatorcontrib>Bolarte-Arteaga, Mario</creatorcontrib><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Farmacija</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alvarado, Angel T</au><au>Chávez, Haydee</au><au>García, Jorge A</au><au>Bendezú, María R</au><au>Surco-Laos, Felipe</au><au>Molina-Cabrera, Aura</au><au>Laos-Anchante, Doris</au><au>Vega-Ramos, Nelly</au><au>Palomino-Jhong, Juan J</au><au>Yarasca-Carlos, Paulina Eliades</au><au>Loja-Herrera, Berta</au><au>Pineda-Pérez, Mario</au><au>Bolarte-Arteaga, Mario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru</atitle><jtitle>Farmacija</jtitle><date>2024-05-14</date><risdate>2024</risdate><volume>71</volume><issue>2</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>0428-0296</issn><eissn>2603-557X</eissn><abstract>Heliotropium arborescens
(Galán de Mera et al. 2022) is a herbaceous plant that contains various biophenolic compounds that exhibit antibacterial property and antioxidant activity that could prevent diseases caused by free radicals. The objective was to study the biophenolic compounds and metal ions associated with the
in vitro
antioxidant and antibacterial activity of the ethanolic extract of
Heliotropium arborescens
L. leaves from the Andean region of Ayacucho-Peru. The total polyphenolic content (TPC) was determined by the Folin-Ciocalteu method; Total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, TFC was expressed in mg quercetin/g sample (mg QE/g). Antioxidant activity by three
in vitro
methods; determination of metals by flame atomic absorption spectrophotometry (FAAS) and antibacterial activity by agar diffusion method. The values obtained from the TPC were 306.80±0.05 mg GAE/g, TFC 111.53±0.25 mg QE/g. Antioxidant activity: DPPH IC
50
37.55±0.04 µL and 65.64±0.10 µL, at 0.47 and 0.93 mg/mL extract, respectively; ABTS 0.20±0.01 mM TEAC/g (0.41 mg/mL extract) and 0.47±0.01 (0.82 mg/mL extract); FRAP 0.24±0.01 mM TEAC/g (0.94 mg/mL extract) and 0.46±0.02 mM TEAC/g (1.88 mg/mL extract). Correlation relationship TFC/DPPH (r=0.9994; R
2
0.9989), TFC/ABTS (r = 0.9572; R
2
0.9998) and TFC/FRAP (r = 09933; R
2
0.9868). Calcium 1.22±0.04, copper 0.77±0.05, magnesium 851.7±3.06 and zinc 20.63±1.19 mg/100 g were found in all cases. Antibacterial activity on
Bacillus subtilis
(ATCC 6633) and
Staphylococcus aureus
(ATCC 25923) with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) > 400 μg/mL. It is concluded that the total biophenolic components and metals of the ethanolic extract of the leaves of
H. arborescens
L. show potential antioxidant activity, and moderate antibacterial activity against
Bacillus subtilis
and
Staphylococcus aureus
.
Graphical abstract
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| language | eng |
| recordid | cdi_gale_infotracmisc_A793710674 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Antibacterial agents Antioxidants Isoflavones |
| title | Biophenolic compounds and metal ions associated with the antioxidant and antibacterial activity of the ethanolic extract of Heliotropium arborescens L. leaves from the Andean region of Ayacucho-Peru |
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