Determination of chemical compositions, antioxidant, DNA cleavage and binding properties of Vincetoxicum tmoleum extract
In the present study, phenolic compounds, antioxidant activities, DNA cleavage and binding effects of Vincetoxicum tmoleum (Vt) collected from west of Anatolia (Manisa city) were investigated. Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents,...
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Veröffentlicht in: | Studia Universitatis Babeș-Bolyai. Chemia 2024-03, Vol.69 (1), p.163-174 |
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description | In the present study, phenolic compounds, antioxidant activities, DNA cleavage and binding effects of Vincetoxicum tmoleum (Vt) collected from west of Anatolia (Manisa city) were investigated. Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing antioxidant power assay) tests. Besides, the DNA cleavage and binding features of V. tmoleum extract were studied using pBR322 DNA and CT-DNA, respectively. Phenolic compounds of the extract were analyzed by high performance liquid chromatography (HPLC-DAD). As a result, V. tmoleum methanol extract was found to have the high total phenolic and total flavonoid content and antioxidant effect. Strong positive correlations were also found between DPPH and TFC (r = 0.995; p < 0.01), and TPC (r = 0.989;p < 0.01), ABTS (r = 0.994;p < 0.01) and FRAP(r=0,995; p < 0.01). Methanol extract of V. tmoleum had large amounts of p- coumaric acid, ferulic acid and protocatechuic acid. While V. tmoleum samples showed weak DNA cleavage activity, they showed DNA binding activity at 50 [micro]M concentration, that is, the potential to be an intercalation agent in this concentration. Keywords: Vincetoxicum tmoleum, antioxidant activity, DNA cleavage, HPLC-DAD |
doi_str_mv | 10.24193/subbchem.2024.1.10 |
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Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing antioxidant power assay) tests. Besides, the DNA cleavage and binding features of V. tmoleum extract were studied using pBR322 DNA and CT-DNA, respectively. Phenolic compounds of the extract were analyzed by high performance liquid chromatography (HPLC-DAD). As a result, V. tmoleum methanol extract was found to have the high total phenolic and total flavonoid content and antioxidant effect. Strong positive correlations were also found between DPPH and TFC (r = 0.995; p < 0.01), and TPC (r = 0.989;p < 0.01), ABTS (r = 0.994;p < 0.01) and FRAP(r=0,995; p < 0.01). Methanol extract of V. tmoleum had large amounts of p- coumaric acid, ferulic acid and protocatechuic acid. While V. tmoleum samples showed weak DNA cleavage activity, they showed DNA binding activity at 50 [micro]M concentration, that is, the potential to be an intercalation agent in this concentration. Keywords: Vincetoxicum tmoleum, antioxidant activity, DNA cleavage, HPLC-DAD</description><identifier>ISSN: 1224-7154</identifier><identifier>EISSN: 2065-9520</identifier><identifier>DOI: 10.24193/subbchem.2024.1.10</identifier><language>eng</language><publisher>Universitatea Babes-Bolyai. Chemia</publisher><subject>Amino acids ; Antioxidants ; Bioflavonoids ; DNA ; Ethylenediaminetetraacetic acid ; Flavones ; Flavonoids ; High performance liquid chromatography ; Methanol</subject><ispartof>Studia Universitatis Babeș-Bolyai. Chemia, 2024-03, Vol.69 (1), p.163-174</ispartof><rights>COPYRIGHT 2024 Universitatea Babes-Bolyai. Chemia</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Selvi, Emine Kilickaya</creatorcontrib><creatorcontrib>Guler, Nilgun</creatorcontrib><creatorcontrib>Guven, Seher</creatorcontrib><creatorcontrib>Makbul, Serdar</creatorcontrib><creatorcontrib>Department of Chemistry and Chemical Processing Technologies, Mustafa Çıkrıkcıoglu Vocational School, Kayseri University, Kayseri, Türkiye</creatorcontrib><creatorcontrib>Department of Biology, Faculty of Sciences and Arts, Recep Tayyip Erdogan University, Rize, Türkiye</creatorcontrib><title>Determination of chemical compositions, antioxidant, DNA cleavage and binding properties of Vincetoxicum tmoleum extract</title><title>Studia Universitatis Babeș-Bolyai. Chemia</title><description>In the present study, phenolic compounds, antioxidant activities, DNA cleavage and binding effects of Vincetoxicum tmoleum (Vt) collected from west of Anatolia (Manisa city) were investigated. Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing antioxidant power assay) tests. Besides, the DNA cleavage and binding features of V. tmoleum extract were studied using pBR322 DNA and CT-DNA, respectively. Phenolic compounds of the extract were analyzed by high performance liquid chromatography (HPLC-DAD). As a result, V. tmoleum methanol extract was found to have the high total phenolic and total flavonoid content and antioxidant effect. Strong positive correlations were also found between DPPH and TFC (r = 0.995; p < 0.01), and TPC (r = 0.989;p < 0.01), ABTS (r = 0.994;p < 0.01) and FRAP(r=0,995; p < 0.01). Methanol extract of V. tmoleum had large amounts of p- coumaric acid, ferulic acid and protocatechuic acid. While V. tmoleum samples showed weak DNA cleavage activity, they showed DNA binding activity at 50 [micro]M concentration, that is, the potential to be an intercalation agent in this concentration. Keywords: Vincetoxicum tmoleum, antioxidant activity, DNA cleavage, HPLC-DAD</description><subject>Amino acids</subject><subject>Antioxidants</subject><subject>Bioflavonoids</subject><subject>DNA</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Flavones</subject><subject>Flavonoids</subject><subject>High performance liquid chromatography</subject><subject>Methanol</subject><issn>1224-7154</issn><issn>2065-9520</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNptUU1PwzAMjRBITGO_gEskrmuJk7Rdj9PGlzTBBbhWaeKMoLaZmg6Nf0_K4ICEfXiW7fcs-RFyCSzlEkpxHfZ1rd-wTTnjMoUU2AmZcJZnSZlxdkomwLlMCsjkOZmF8M4YA-CZzNmEHNY4YN-6Tg3Od9RbOio5rRqqfbvzwY39MKeqi8XBmYhzun5cUt2g-lBbjBNDa9cZ123prvc77AeHYZR6dZ3GIbL0vqVD6xuMiIehV3q4IGdWNQFnPzglL7c3z6v7ZPN097BabhItAIbEZKwos9IKGaOQJgfLhDU151mmpeQcwIicl1YbK2GxqMFiWSMqtihrLpSYkquj7lY1WLnO-vF864KulkXJZV6wvIhb6T9bMc34DN-hdbH_hyCOBN37EHq01a53reo_K2DVty3Vry3VaEsFcSC-AKgQg40</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Selvi, Emine Kilickaya</creator><creator>Guler, Nilgun</creator><creator>Guven, Seher</creator><creator>Makbul, Serdar</creator><general>Universitatea Babes-Bolyai. Chemia</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20240301</creationdate><title>Determination of chemical compositions, antioxidant, DNA cleavage and binding properties of Vincetoxicum tmoleum extract</title><author>Selvi, Emine Kilickaya ; Guler, Nilgun ; Guven, Seher ; Makbul, Serdar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-d507959f3444474d61f03fdb2255c442211d3629fcdf4188b1fe9beea089b23a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amino acids</topic><topic>Antioxidants</topic><topic>Bioflavonoids</topic><topic>DNA</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Flavones</topic><topic>Flavonoids</topic><topic>High performance liquid chromatography</topic><topic>Methanol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Selvi, Emine Kilickaya</creatorcontrib><creatorcontrib>Guler, Nilgun</creatorcontrib><creatorcontrib>Guven, Seher</creatorcontrib><creatorcontrib>Makbul, Serdar</creatorcontrib><creatorcontrib>Department of Chemistry and Chemical Processing Technologies, Mustafa Çıkrıkcıoglu Vocational School, Kayseri University, Kayseri, Türkiye</creatorcontrib><creatorcontrib>Department of Biology, Faculty of Sciences and Arts, Recep Tayyip Erdogan University, Rize, Türkiye</creatorcontrib><collection>CrossRef</collection><jtitle>Studia Universitatis Babeș-Bolyai. Chemia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Selvi, Emine Kilickaya</au><au>Guler, Nilgun</au><au>Guven, Seher</au><au>Makbul, Serdar</au><aucorp>Department of Chemistry and Chemical Processing Technologies, Mustafa Çıkrıkcıoglu Vocational School, Kayseri University, Kayseri, Türkiye</aucorp><aucorp>Department of Biology, Faculty of Sciences and Arts, Recep Tayyip Erdogan University, Rize, Türkiye</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of chemical compositions, antioxidant, DNA cleavage and binding properties of Vincetoxicum tmoleum extract</atitle><jtitle>Studia Universitatis Babeș-Bolyai. Chemia</jtitle><date>2024-03-01</date><risdate>2024</risdate><volume>69</volume><issue>1</issue><spage>163</spage><epage>174</epage><pages>163-174</pages><issn>1224-7154</issn><eissn>2065-9520</eissn><abstract>In the present study, phenolic compounds, antioxidant activities, DNA cleavage and binding effects of Vincetoxicum tmoleum (Vt) collected from west of Anatolia (Manisa city) were investigated. Antioxidant potentials of the extracts were characterized with their total phenolic and flavonoid contents, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid), and FRAP (ferric reducing antioxidant power assay) tests. Besides, the DNA cleavage and binding features of V. tmoleum extract were studied using pBR322 DNA and CT-DNA, respectively. Phenolic compounds of the extract were analyzed by high performance liquid chromatography (HPLC-DAD). As a result, V. tmoleum methanol extract was found to have the high total phenolic and total flavonoid content and antioxidant effect. Strong positive correlations were also found between DPPH and TFC (r = 0.995; p < 0.01), and TPC (r = 0.989;p < 0.01), ABTS (r = 0.994;p < 0.01) and FRAP(r=0,995; p < 0.01). Methanol extract of V. tmoleum had large amounts of p- coumaric acid, ferulic acid and protocatechuic acid. While V. tmoleum samples showed weak DNA cleavage activity, they showed DNA binding activity at 50 [micro]M concentration, that is, the potential to be an intercalation agent in this concentration. Keywords: Vincetoxicum tmoleum, antioxidant activity, DNA cleavage, HPLC-DAD</abstract><pub>Universitatea Babes-Bolyai. Chemia</pub><doi>10.24193/subbchem.2024.1.10</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino acids Antioxidants Bioflavonoids DNA Ethylenediaminetetraacetic acid Flavones Flavonoids High performance liquid chromatography Methanol |
title | Determination of chemical compositions, antioxidant, DNA cleavage and binding properties of Vincetoxicum tmoleum extract |
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