Studies on Antioxidant and Hepatoprotective Activities of Extracts and Active Fraction of Pachypodanthium staudtii Stem Bark
This study evaluates the antioxidant and hepatoprotective activities of the stem bark extracts (methanol, PSM and dichloromethane, PSD) and active fraction of Pachypodanthium staudtii. In-vitro antioxidant activity was determined by DPPH, total phenolic content (TPC) and reducing power assay (RPA),...
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| Veröffentlicht in: | Pakistan journal of scientific and industrial research. Series B: biological sciences 2024-01, Vol.67 (1), p.38 |
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| container_title | Pakistan journal of scientific and industrial research. Series B: biological sciences |
| container_volume | 67 |
| creator | Okorafor, Ezinne Chinecherem Kenne, Michel Tchimene Obi, Bonaventure Chinonso Obonga, Wilfred Ofem Akunne, Theophine Chinwuba Ezike, Adaobi Chioma |
| description | This study evaluates the antioxidant and hepatoprotective activities of the stem bark extracts (methanol, PSM and dichloromethane, PSD) and active fraction of Pachypodanthium staudtii. In-vitro antioxidant activity was determined by DPPH, total phenolic content (TPC) and reducing power assay (RPA), while the hepatoprotective activity was verified against [CCl.sub.4]-induced acute hepatotoxicity in rats. Serum biomarkers (ALT, AST, ALP), in-vivo antioxidant (superoxide dismutase, SOD and catalase, CAT) and malondialdehyde (MDA) levels were estimated using standard models. Histopathological studies were carried out on the liver isolates. The result showed that PSM significantly inhibited DPPH ([IC.sub.50]=63.70 [micro]g/mL) compared to PSD ([IC.sub.50]=1591.58 [micro]g/mL). PSM showed a higher phenolic content of 2536.26 mgGAE/g compared to 1916.78 mgGAE/g for PSD. Fraction A showed a phenolic content of 553.01 mgGAE/g. Significant (P |
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In-vitro antioxidant activity was determined by DPPH, total phenolic content (TPC) and reducing power assay (RPA), while the hepatoprotective activity was verified against [CCl.sub.4]-induced acute hepatotoxicity in rats. Serum biomarkers (ALT, AST, ALP), in-vivo antioxidant (superoxide dismutase, SOD and catalase, CAT) and malondialdehyde (MDA) levels were estimated using standard models. Histopathological studies were carried out on the liver isolates. The result showed that PSM significantly inhibited DPPH ([IC.sub.50]=63.70 [micro]g/mL) compared to PSD ([IC.sub.50]=1591.58 [micro]g/mL). PSM showed a higher phenolic content of 2536.26 mgGAE/g compared to 1916.78 mgGAE/g for PSD. Fraction A showed a phenolic content of 553.01 mgGAE/g. Significant (P<0.05) decrease in hepatic biomarkers (ALT, AST, ALP) was observed at varying degrees in PSM and PSD treated groups compared to [CCl.sub.4]-control group. Fraction A showed significant (P<0.05) effect on hepatic biomarkers (ALT, AST, ALP), in vivo antioxidant (SOD, CAT) and MDA levels compared to the [CCl.sub.4]-control group. Histopathology studies revealed restoration of liver architecture and healing from [CCl.sub.4] intoxication in the liver. Keywords: P staudtii, methanol extract, dichloromethane, antioxidant, hepatoprotective</description><identifier>ISSN: 2221-6421</identifier><language>eng</language><publisher>Pakistan Council of Scientific and Industrial Research</publisher><subject>Analysis ; Antioxidants ; Health aspects ; Identification and classification ; Magnoliidae</subject><ispartof>Pakistan journal of scientific and industrial research. 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Series B: biological sciences</title><description>This study evaluates the antioxidant and hepatoprotective activities of the stem bark extracts (methanol, PSM and dichloromethane, PSD) and active fraction of Pachypodanthium staudtii. In-vitro antioxidant activity was determined by DPPH, total phenolic content (TPC) and reducing power assay (RPA), while the hepatoprotective activity was verified against [CCl.sub.4]-induced acute hepatotoxicity in rats. Serum biomarkers (ALT, AST, ALP), in-vivo antioxidant (superoxide dismutase, SOD and catalase, CAT) and malondialdehyde (MDA) levels were estimated using standard models. Histopathological studies were carried out on the liver isolates. The result showed that PSM significantly inhibited DPPH ([IC.sub.50]=63.70 [micro]g/mL) compared to PSD ([IC.sub.50]=1591.58 [micro]g/mL). PSM showed a higher phenolic content of 2536.26 mgGAE/g compared to 1916.78 mgGAE/g for PSD. Fraction A showed a phenolic content of 553.01 mgGAE/g. Significant (P<0.05) decrease in hepatic biomarkers (ALT, AST, ALP) was observed at varying degrees in PSM and PSD treated groups compared to [CCl.sub.4]-control group. Fraction A showed significant (P<0.05) effect on hepatic biomarkers (ALT, AST, ALP), in vivo antioxidant (SOD, CAT) and MDA levels compared to the [CCl.sub.4]-control group. Histopathology studies revealed restoration of liver architecture and healing from [CCl.sub.4] intoxication in the liver. Keywords: P staudtii, methanol extract, dichloromethane, antioxidant, hepatoprotective</description><subject>Analysis</subject><subject>Antioxidants</subject><subject>Health aspects</subject><subject>Identification and classification</subject><subject>Magnoliidae</subject><issn>2221-6421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptj09LAzEQxfegYKn9DgHPK0n2_3EtrRUKCu29TJNJO9rdLJusVPDDm209eJA5PHjze4-Zm2gipRRxnkpxF82ce-ecCynSjGeT6HvjB03omG1Z3XqyZ9LQegatZivswNuutx6Vp09k9SjkL7hhi7PvQXl3YesrsRwdCl1h_wbq-NXZse5IQ8Och0F7Irbx2LAn6D_uo1sDJ4ezX51G2-ViO1_F69fnl3m9jg95kcYJpBnq0kjJww9c6CrJiqKUHCHhuBdQFQqUFDlWWBq1TwCNRlGEiNKJqJJp9HCtPcAJd9QaOx7ekFO7uiirnOdCpIF6_IcKo7EhZVs0FPw_gR-gzms0</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Okorafor, Ezinne Chinecherem</creator><creator>Kenne, Michel Tchimene</creator><creator>Obi, Bonaventure Chinonso</creator><creator>Obonga, Wilfred Ofem</creator><creator>Akunne, Theophine Chinwuba</creator><creator>Ezike, Adaobi Chioma</creator><general>Pakistan Council of Scientific and Industrial Research</general><scope/></search><sort><creationdate>20240101</creationdate><title>Studies on Antioxidant and Hepatoprotective Activities of Extracts and Active Fraction of Pachypodanthium staudtii Stem Bark</title><author>Okorafor, Ezinne Chinecherem ; Kenne, Michel Tchimene ; Obi, Bonaventure Chinonso ; Obonga, Wilfred Ofem ; Akunne, Theophine Chinwuba ; Ezike, Adaobi Chioma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g674-3a45ed8f22064201d93577820ea30eb1a97cac216e9e8fcb3aefde17d8fcd3193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analysis</topic><topic>Antioxidants</topic><topic>Health aspects</topic><topic>Identification and classification</topic><topic>Magnoliidae</topic><toplevel>online_resources</toplevel><creatorcontrib>Okorafor, Ezinne Chinecherem</creatorcontrib><creatorcontrib>Kenne, Michel Tchimene</creatorcontrib><creatorcontrib>Obi, Bonaventure Chinonso</creatorcontrib><creatorcontrib>Obonga, Wilfred Ofem</creatorcontrib><creatorcontrib>Akunne, Theophine Chinwuba</creatorcontrib><creatorcontrib>Ezike, Adaobi Chioma</creatorcontrib><jtitle>Pakistan journal of scientific and industrial research. Series B: biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okorafor, Ezinne Chinecherem</au><au>Kenne, Michel Tchimene</au><au>Obi, Bonaventure Chinonso</au><au>Obonga, Wilfred Ofem</au><au>Akunne, Theophine Chinwuba</au><au>Ezike, Adaobi Chioma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on Antioxidant and Hepatoprotective Activities of Extracts and Active Fraction of Pachypodanthium staudtii Stem Bark</atitle><jtitle>Pakistan journal of scientific and industrial research. Series B: biological sciences</jtitle><date>2024-01-01</date><risdate>2024</risdate><volume>67</volume><issue>1</issue><spage>38</spage><pages>38-</pages><issn>2221-6421</issn><abstract>This study evaluates the antioxidant and hepatoprotective activities of the stem bark extracts (methanol, PSM and dichloromethane, PSD) and active fraction of Pachypodanthium staudtii. In-vitro antioxidant activity was determined by DPPH, total phenolic content (TPC) and reducing power assay (RPA), while the hepatoprotective activity was verified against [CCl.sub.4]-induced acute hepatotoxicity in rats. Serum biomarkers (ALT, AST, ALP), in-vivo antioxidant (superoxide dismutase, SOD and catalase, CAT) and malondialdehyde (MDA) levels were estimated using standard models. Histopathological studies were carried out on the liver isolates. The result showed that PSM significantly inhibited DPPH ([IC.sub.50]=63.70 [micro]g/mL) compared to PSD ([IC.sub.50]=1591.58 [micro]g/mL). PSM showed a higher phenolic content of 2536.26 mgGAE/g compared to 1916.78 mgGAE/g for PSD. Fraction A showed a phenolic content of 553.01 mgGAE/g. Significant (P<0.05) decrease in hepatic biomarkers (ALT, AST, ALP) was observed at varying degrees in PSM and PSD treated groups compared to [CCl.sub.4]-control group. Fraction A showed significant (P<0.05) effect on hepatic biomarkers (ALT, AST, ALP), in vivo antioxidant (SOD, CAT) and MDA levels compared to the [CCl.sub.4]-control group. Histopathology studies revealed restoration of liver architecture and healing from [CCl.sub.4] intoxication in the liver. Keywords: P staudtii, methanol extract, dichloromethane, antioxidant, hepatoprotective</abstract><pub>Pakistan Council of Scientific and Industrial Research</pub></addata></record> |
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| ispartof | Pakistan journal of scientific and industrial research. Series B: biological sciences, 2024-01, Vol.67 (1), p.38 |
| issn | 2221-6421 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Analysis Antioxidants Health aspects Identification and classification Magnoliidae |
| title | Studies on Antioxidant and Hepatoprotective Activities of Extracts and Active Fraction of Pachypodanthium staudtii Stem Bark |
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