Improvement in the Stability and Enzymatic Activity of IPleurotus sapidus/I Lipoxygenase Dissolved in Natural Deep Eutectic Solvents

Improvement in the Stability and Enzymatic Activity of IPleurotus sapidus/I Lipoxygenase Dissolved in Natural Deep Eutectic Solvents

Natural deep eutectic solvents (NADESs) can serve as solvents for enzymes, are biodegradable, and have low toxicities. Eight NADESs with different hydrogen bond acceptors and donors were tested to improve the stability and activity of a lipoxygenase from Basidiomycete Pleurotus sapidus (LOX[sub.PSA]...

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Veröffentlicht in:Life (Basel, Switzerland) Switzerland), 2024-02, Vol.14 (2)
Hauptverfasser: Garbe, Maria, Lehmann, Leander Tom, Berger, Ralf Günter, Ersoy, Franziska
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Sprache:eng
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Biological products
Chemical properties
Chlorides
Dichloropropane
Oxidoreductases
Production processes
Solvents
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container_issue 2
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container_title Life (Basel, Switzerland)
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creator Garbe, Maria
Lehmann, Leander Tom
Berger, Ralf Günter
Ersoy, Franziska
description Natural deep eutectic solvents (NADESs) can serve as solvents for enzymes, are biodegradable, and have low toxicities. Eight NADESs with different hydrogen bond acceptors and donors were tested to improve the stability and activity of a lipoxygenase from Basidiomycete Pleurotus sapidus (LOX[sub.PSA] ). Betaine:sorbitol:water (1:1:3, BSorbW) and betaine:ethylene glycol (1:3, BEtGly) had the best impact on the peroxidation of linoleic acid and the side reaction of piperine to the vanilla-like scented compound piperonal. The yield of piperonal in NADESs increased by 43% in BSorbW and 40% in BEtGly compared to the control. The addition of BSorbW also enhanced the enzyme’s stability at various temperatures and increased its activity during incubation at 60 °C. The demonstrated improvement in lipoxygenase activity and stability indicates versatile applications in industry, expanding the potential uses of the enzyme.
doi_str_mv 10.3390/life14020271
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subjects Biological products
Chemical properties
Chlorides
Dichloropropane
Oxidoreductases
Production processes
Solvents
title Improvement in the Stability and Enzymatic Activity of IPleurotus sapidus/I Lipoxygenase Dissolved in Natural Deep Eutectic Solvents
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