Methyl [beta]-Cyclodextrin-sperm-mediated gene editing (MBCD-SMGE): a simple and efficient method for targeted mutant mouse production

Background Generating targeted mutant mice is a crucial technology in biomedical research. This study focuses on optimizing the CRISPR/Cas9 system uptake into sperm cells using the methyl [beta]-cyclodextrin-sperm-mediated gene transfer (MBCD-SMGT) technique to generate targeted mutant blastocysts a...

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Veröffentlicht in:	Biological procedures online 2024-01, Vol.26 (1)
Hauptverfasser:	Moradbeigi, Parisa, Hosseini, Sara, Salehi, Mohammad, Mogheiseh, Asghar
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animal experimentation Cyclodextrins Embryonic development Genes Medical research Medicine, Experimental Methods Production processes Spermatozoa
Online-Zugang:	Volltext
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