Isolation and Characterization of Anti-Inflammatory Compounds from IFicus microcarpa/I L.f. Stem Bark
The anti-inflammatory effect of the ethyl acetate extract of F. microcarpa bark (EAFMB) was investigated in acute and chronic (21 days) inflammation induced in Wistar albino rats. EAFMB (200 mg/kg b.w.) exhibited comparable anti-inflammatory effects to the reference drug, with a reduction of 59.48%...
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| Veröffentlicht in: | Plants (Basel) 2023-09, Vol.12 (18) |
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| creator | Kalaskar, Mohan Redasani, Vivek Ayyanar, Muniappan Ghante, Mahavir Firke, Sandip Agrawal, Kapil Ghawate, Vilas Surana, Sanjay Alarifi, Saud Chikhale, Rupesh Gurav, Shailendra |
| description | The anti-inflammatory effect of the ethyl acetate extract of F. microcarpa bark (EAFMB) was investigated in acute and chronic (21 days) inflammation induced in Wistar albino rats. EAFMB (200 mg/kg b.w.) exhibited comparable anti-inflammatory effects to the reference drug, with a reduction of 59.48% at 4 h in acute inflammation and 83.96% on day 21 in chronic inflammation. Bioassay-guided fractionation using DPPH radical scavenging activity led to isolating and identifying three compounds from EAFMB: oleanolic acid, catechin, and p-hydroxycinnamic acid. All these compounds demonstrated the concentration-dependent inhibition of COX enzymes and the protection of egg albumin from heat-induced denaturation. Catechin exhibited the highest COX inhibition (COX-1 and COX-2 IC[sub.50] = 9.02 and 50.38 μM, respectively) and anti-denaturation effect (IC[sub.50] = 27.13 μg/mL) compared to oleanolic acid and p-hydroxycinnamic acid. These isolated compounds are likely responsible for the anti-inflammatory activities of F. microcarpa bark. |
| doi_str_mv | 10.3390/plants12183248 |
| format | Article |
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EAFMB (200 mg/kg b.w.) exhibited comparable anti-inflammatory effects to the reference drug, with a reduction of 59.48% at 4 h in acute inflammation and 83.96% on day 21 in chronic inflammation. Bioassay-guided fractionation using DPPH radical scavenging activity led to isolating and identifying three compounds from EAFMB: oleanolic acid, catechin, and p-hydroxycinnamic acid. All these compounds demonstrated the concentration-dependent inhibition of COX enzymes and the protection of egg albumin from heat-induced denaturation. Catechin exhibited the highest COX inhibition (COX-1 and COX-2 IC[sub.50] = 9.02 and 50.38 μM, respectively) and anti-denaturation effect (IC[sub.50] = 27.13 μg/mL) compared to oleanolic acid and p-hydroxycinnamic acid. 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These isolated compounds are likely responsible for the anti-inflammatory activities of F. microcarpa bark.</description><subject>Anti-inflammatory drugs</subject><subject>Arthritis</subject><subject>Bark</subject><subject>Chemical properties</subject><subject>Ficus (Plants)</subject><subject>Health aspects</subject><subject>Identification and classification</subject><subject>Inflammation</subject><subject>Materia medica, Vegetable</subject><subject>Pharmaceutical research</subject><subject>Phytochemicals</subject><subject>Plant extracts</subject><issn>2223-7747</issn><issn>2223-7747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNptj8tLAzEQxoMoWGqvngOePOx289jXsS62LhQEq-cym0eN7iZlk4L61xuohxacGZjh4_cNfAjdkixlrM7m-x5s8ISSilFeXaAJpZQlZcnLy5P7Gs28_8hiVXFIMUGq9a6HYJzFYCVu3mEEEdRofo6i03hhg0laq3sYBghu_MaNG_buYKXHenQDbpdGHDwejBidgHEP8xavU53iTVADfoDx8wZdaei9mv3tKXpbPr42T8n6edU2i3WyIxlhCYU8J1XFAXTeZbxQwCUFwjpGO0EoFETKUnaFlirLFcQYNdSMS1kTJamQbIrujn930KutsdqFGGcwXmwXZUkqQjllkUr_oWJLFTM4q7SJ-pnh_swQmaC-wg4O3m_bzcsp-wsQr3co</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Kalaskar, Mohan</creator><creator>Redasani, Vivek</creator><creator>Ayyanar, Muniappan</creator><creator>Ghante, Mahavir</creator><creator>Firke, Sandip</creator><creator>Agrawal, Kapil</creator><creator>Ghawate, Vilas</creator><creator>Surana, Sanjay</creator><creator>Alarifi, Saud</creator><creator>Chikhale, Rupesh</creator><creator>Gurav, Shailendra</creator><general>MDPI AG</general><scope>ISR</scope></search><sort><creationdate>20230901</creationdate><title>Isolation and Characterization of Anti-Inflammatory Compounds from IFicus microcarpa/I L.f. 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Stem Bark</atitle><jtitle>Plants (Basel)</jtitle><date>2023-09-01</date><risdate>2023</risdate><volume>12</volume><issue>18</issue><issn>2223-7747</issn><eissn>2223-7747</eissn><abstract>The anti-inflammatory effect of the ethyl acetate extract of F. microcarpa bark (EAFMB) was investigated in acute and chronic (21 days) inflammation induced in Wistar albino rats. EAFMB (200 mg/kg b.w.) exhibited comparable anti-inflammatory effects to the reference drug, with a reduction of 59.48% at 4 h in acute inflammation and 83.96% on day 21 in chronic inflammation. Bioassay-guided fractionation using DPPH radical scavenging activity led to isolating and identifying three compounds from EAFMB: oleanolic acid, catechin, and p-hydroxycinnamic acid. All these compounds demonstrated the concentration-dependent inhibition of COX enzymes and the protection of egg albumin from heat-induced denaturation. Catechin exhibited the highest COX inhibition (COX-1 and COX-2 IC[sub.50] = 9.02 and 50.38 μM, respectively) and anti-denaturation effect (IC[sub.50] = 27.13 μg/mL) compared to oleanolic acid and p-hydroxycinnamic acid. These isolated compounds are likely responsible for the anti-inflammatory activities of F. microcarpa bark.</abstract><pub>MDPI AG</pub><doi>10.3390/plants12183248</doi></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Anti-inflammatory drugs Arthritis Bark Chemical properties Ficus (Plants) Health aspects Identification and classification Inflammation Materia medica, Vegetable Pharmaceutical research Phytochemicals Plant extracts |
| title | Isolation and Characterization of Anti-Inflammatory Compounds from IFicus microcarpa/I L.f. Stem Bark |
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