Adjuvanted Fusion Protein Vaccine Induces Durable Immunity to IOnchocerca volvulus/I in Mice and Non-Human Primates
Onchocerciasis remains a debilitating neglected tropical disease. Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consis...
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| Veröffentlicht in: | Vaccines (Basel) 2023-07, Vol.11 (7) |
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| creator | Ryan, Nathan M Hess, Jessica A Robertson, Erica J Tricoche, Nancy Turner, Cheri Davis, Jenn Petrovsky, Nikolai Ferguson, Melissa Rinaldi, William J Wong, Valerie M Shimada, Ayako Zhan, Bin Bottazzi, Maria Elena Makepeace, Benjamin L Gray, Sean A Carter, Darrick Lustigman, Sara Abraham, David |
| description | Onchocerciasis remains a debilitating neglected tropical disease. Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consisting of a fusion of two O. volvulus protein antigens, Ov-103 and Ov-RAL-2 (Ov-FUS-1), and three different adjuvants: Advax-CpG, alum, and AlT4. All vaccine formulations induced high antigen-specific IgG titers in both mice and NHPs. Challenging mice with O. volvulus L3 contained within subcutaneous diffusion chambers demonstrated that Ov-FUS-1/Advax-CpG-immunized animals developed protective immunity, durable for at least 11 weeks. Passive transfer of sera, collected at several time points, from both mice and NHPs immunized with Ov-FUS-1/Advax-CpG transferred protection to naïve mice. These results demonstrate that Ov-FUS-1 with the adjuvant Advax-CpG induces durable protective immunity against O. volvulus in mice and NHPs that is mediated by vaccine-induced humoral factors. |
| doi_str_mv | 10.3390/vaccines11071212 |
| format | Article |
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Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consisting of a fusion of two O. volvulus protein antigens, Ov-103 and Ov-RAL-2 (Ov-FUS-1), and three different adjuvants: Advax-CpG, alum, and AlT4. All vaccine formulations induced high antigen-specific IgG titers in both mice and NHPs. Challenging mice with O. volvulus L3 contained within subcutaneous diffusion chambers demonstrated that Ov-FUS-1/Advax-CpG-immunized animals developed protective immunity, durable for at least 11 weeks. Passive transfer of sera, collected at several time points, from both mice and NHPs immunized with Ov-FUS-1/Advax-CpG transferred protection to naïve mice. 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Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consisting of a fusion of two O. volvulus protein antigens, Ov-103 and Ov-RAL-2 (Ov-FUS-1), and three different adjuvants: Advax-CpG, alum, and AlT4. All vaccine formulations induced high antigen-specific IgG titers in both mice and NHPs. Challenging mice with O. volvulus L3 contained within subcutaneous diffusion chambers demonstrated that Ov-FUS-1/Advax-CpG-immunized animals developed protective immunity, durable for at least 11 weeks. Passive transfer of sera, collected at several time points, from both mice and NHPs immunized with Ov-FUS-1/Advax-CpG transferred protection to naïve mice. These results demonstrate that Ov-FUS-1 with the adjuvant Advax-CpG induces durable protective immunity against O. volvulus in mice and NHPs that is mediated by vaccine-induced humoral factors.</description><subject>Design and construction</subject><subject>Evaluation</subject><subject>Immunity</subject><subject>Vaccines</subject><issn>2076-393X</issn><issn>2076-393X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptjL1rwzAQxUVpoSHN3lHQ2Yk-EskeQ9o0hrTpEEq3oI9zqmBLYFmG_vd1SIcMvTfc3eO9H0KPlEw5L8isV8Y4D5FSIimj7AaNGJEi4wX_ur2679EkxhMZpqA8F3KE4tKeUq98BxavU3TB4482dOA8_rxAceltMhDxc2qVroe_aZJ33Q_uAi533nwHA61RuA91n-oUZyUe2m_OAFbe4vfgs01q1BnsGtVBfEB3laojTP72GO3XL_vVJtvuXsvVcpsdheRZJTm3GkxuhTZaLeYkrwpCcq1EDoxJaRkRBRGMVYIKrjm1mmtmiJhbKzjwMXq6YI-qhoPzVehaZRoXzWEpFwWbUyb4kJr-kxpkoXEmeKjc4F8VfgFKj27R</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Ryan, Nathan M</creator><creator>Hess, Jessica A</creator><creator>Robertson, Erica J</creator><creator>Tricoche, Nancy</creator><creator>Turner, Cheri</creator><creator>Davis, Jenn</creator><creator>Petrovsky, Nikolai</creator><creator>Ferguson, Melissa</creator><creator>Rinaldi, William J</creator><creator>Wong, Valerie M</creator><creator>Shimada, Ayako</creator><creator>Zhan, Bin</creator><creator>Bottazzi, Maria Elena</creator><creator>Makepeace, Benjamin L</creator><creator>Gray, Sean A</creator><creator>Carter, Darrick</creator><creator>Lustigman, Sara</creator><creator>Abraham, David</creator><general>MDPI AG</general><scope/></search><sort><creationdate>20230701</creationdate><title>Adjuvanted Fusion Protein Vaccine Induces Durable Immunity to IOnchocerca volvulus/I in Mice and Non-Human Primates</title><author>Ryan, Nathan M ; Hess, Jessica A ; Robertson, Erica J ; Tricoche, Nancy ; Turner, Cheri ; Davis, Jenn ; Petrovsky, Nikolai ; Ferguson, Melissa ; Rinaldi, William J ; Wong, Valerie M ; Shimada, Ayako ; Zhan, Bin ; Bottazzi, Maria Elena ; Makepeace, Benjamin L ; Gray, Sean A ; Carter, Darrick ; Lustigman, Sara ; Abraham, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g673-f733dbec8d6bcba5408f9008ba68e2277d20690622f6163b31db3b2c064dd63e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Design and construction</topic><topic>Evaluation</topic><topic>Immunity</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ryan, Nathan M</creatorcontrib><creatorcontrib>Hess, Jessica A</creatorcontrib><creatorcontrib>Robertson, Erica J</creatorcontrib><creatorcontrib>Tricoche, Nancy</creatorcontrib><creatorcontrib>Turner, Cheri</creatorcontrib><creatorcontrib>Davis, Jenn</creatorcontrib><creatorcontrib>Petrovsky, Nikolai</creatorcontrib><creatorcontrib>Ferguson, Melissa</creatorcontrib><creatorcontrib>Rinaldi, William J</creatorcontrib><creatorcontrib>Wong, Valerie M</creatorcontrib><creatorcontrib>Shimada, Ayako</creatorcontrib><creatorcontrib>Zhan, Bin</creatorcontrib><creatorcontrib>Bottazzi, Maria Elena</creatorcontrib><creatorcontrib>Makepeace, Benjamin L</creatorcontrib><creatorcontrib>Gray, Sean A</creatorcontrib><creatorcontrib>Carter, Darrick</creatorcontrib><creatorcontrib>Lustigman, Sara</creatorcontrib><creatorcontrib>Abraham, David</creatorcontrib><jtitle>Vaccines (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ryan, Nathan M</au><au>Hess, Jessica A</au><au>Robertson, Erica J</au><au>Tricoche, Nancy</au><au>Turner, Cheri</au><au>Davis, Jenn</au><au>Petrovsky, Nikolai</au><au>Ferguson, Melissa</au><au>Rinaldi, William J</au><au>Wong, Valerie M</au><au>Shimada, Ayako</au><au>Zhan, Bin</au><au>Bottazzi, Maria Elena</au><au>Makepeace, Benjamin L</au><au>Gray, Sean A</au><au>Carter, Darrick</au><au>Lustigman, Sara</au><au>Abraham, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adjuvanted Fusion Protein Vaccine Induces Durable Immunity to IOnchocerca volvulus/I in Mice and Non-Human Primates</atitle><jtitle>Vaccines (Basel)</jtitle><date>2023-07-01</date><risdate>2023</risdate><volume>11</volume><issue>7</issue><issn>2076-393X</issn><eissn>2076-393X</eissn><abstract>Onchocerciasis remains a debilitating neglected tropical disease. Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consisting of a fusion of two O. volvulus protein antigens, Ov-103 and Ov-RAL-2 (Ov-FUS-1), and three different adjuvants: Advax-CpG, alum, and AlT4. All vaccine formulations induced high antigen-specific IgG titers in both mice and NHPs. Challenging mice with O. volvulus L3 contained within subcutaneous diffusion chambers demonstrated that Ov-FUS-1/Advax-CpG-immunized animals developed protective immunity, durable for at least 11 weeks. Passive transfer of sera, collected at several time points, from both mice and NHPs immunized with Ov-FUS-1/Advax-CpG transferred protection to naïve mice. These results demonstrate that Ov-FUS-1 with the adjuvant Advax-CpG induces durable protective immunity against O. volvulus in mice and NHPs that is mediated by vaccine-induced humoral factors.</abstract><pub>MDPI AG</pub><doi>10.3390/vaccines11071212</doi></addata></record> |
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| ispartof | Vaccines (Basel), 2023-07, Vol.11 (7) |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Design and construction Evaluation Immunity Vaccines |
| title | Adjuvanted Fusion Protein Vaccine Induces Durable Immunity to IOnchocerca volvulus/I in Mice and Non-Human Primates |
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