Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in INicotiana tabacum/I
Mineral nutrition plays an important role in crop growth, yield and quality. MiR156 is a regulatory hub for growth and development. To date, the understanding of miR156-mediated mineral homeostasis is limited. In this study, we overexpressed Nta-miR156a in the tobacco cultivar TN90 and analyzed the...
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| Veröffentlicht in: | Plants (Basel) 2023-04, Vol.12 (9) |
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| container_title | Plants (Basel) |
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| creator | Liu, Wanhong Ji, Xue Cao, Hanping Huo, Chunsong He, Linshen Peng, Xiang Yang, Ya Yang, Fang Xiong, Shu |
| description | Mineral nutrition plays an important role in crop growth, yield and quality. MiR156 is a regulatory hub for growth and development. To date, the understanding of miR156-mediated mineral homeostasis is limited. In this study, we overexpressed Nta-miR156a in the tobacco cultivar TN90 and analyzed the effects of miR156 on mineral element homeostasis in tobacco by comparative transcriptome analysis. The results showed that the overexpression of miR156a caused significant morphological changes in transgenic tobacco. Chlorophyll and three anti-resistance markers, proline, total phenolics, and total flavonoids, were altered due to increased miR156 expression levels. Interestingly, the distribution of Cu, Mn, Zn, and Fe in different tissues of transgenic tobacco was disordered compared with that of the wild type. Comparative transcriptome analysis showed that the overexpression of miR156 resulted in 2656 significantly differentially expressed genes. The expression levels of several metal-transport-related genes, such as NtABC, NtZIP, NtHMA, and NtCAX, were significantly increased or decreased in transgenic tobacco. These results suggest that miR156 plays an essential role in regulating mineral homeostasis. Our study provides a new perspective for the further study of mineral nutrient homeostasis in plants. |
| doi_str_mv | 10.3390/plants12091739 |
| format | Article |
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MiR156 is a regulatory hub for growth and development. To date, the understanding of miR156-mediated mineral homeostasis is limited. In this study, we overexpressed Nta-miR156a in the tobacco cultivar TN90 and analyzed the effects of miR156 on mineral element homeostasis in tobacco by comparative transcriptome analysis. The results showed that the overexpression of miR156a caused significant morphological changes in transgenic tobacco. Chlorophyll and three anti-resistance markers, proline, total phenolics, and total flavonoids, were altered due to increased miR156 expression levels. Interestingly, the distribution of Cu, Mn, Zn, and Fe in different tissues of transgenic tobacco was disordered compared with that of the wild type. Comparative transcriptome analysis showed that the overexpression of miR156 resulted in 2656 significantly differentially expressed genes. The expression levels of several metal-transport-related genes, such as NtABC, NtZIP, NtHMA, and NtCAX, were significantly increased or decreased in transgenic tobacco. These results suggest that miR156 plays an essential role in regulating mineral homeostasis. Our study provides a new perspective for the further study of mineral nutrient homeostasis in plants.</description><identifier>ISSN: 2223-7747</identifier><identifier>EISSN: 2223-7747</identifier><identifier>DOI: 10.3390/plants12091739</identifier><language>eng</language><publisher>MDPI AG</publisher><subject>Genetic aspects ; Homeostasis ; MicroRNA ; Nutritional aspects ; Physiological aspects ; Tobacco (Plant)</subject><ispartof>Plants (Basel), 2023-04, Vol.12 (9)</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Liu, Wanhong</creatorcontrib><creatorcontrib>Ji, Xue</creatorcontrib><creatorcontrib>Cao, Hanping</creatorcontrib><creatorcontrib>Huo, Chunsong</creatorcontrib><creatorcontrib>He, Linshen</creatorcontrib><creatorcontrib>Peng, Xiang</creatorcontrib><creatorcontrib>Yang, Ya</creatorcontrib><creatorcontrib>Yang, Fang</creatorcontrib><creatorcontrib>Xiong, Shu</creatorcontrib><title>Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in INicotiana tabacum/I</title><title>Plants (Basel)</title><description>Mineral nutrition plays an important role in crop growth, yield and quality. MiR156 is a regulatory hub for growth and development. To date, the understanding of miR156-mediated mineral homeostasis is limited. In this study, we overexpressed Nta-miR156a in the tobacco cultivar TN90 and analyzed the effects of miR156 on mineral element homeostasis in tobacco by comparative transcriptome analysis. The results showed that the overexpression of miR156a caused significant morphological changes in transgenic tobacco. Chlorophyll and three anti-resistance markers, proline, total phenolics, and total flavonoids, were altered due to increased miR156 expression levels. Interestingly, the distribution of Cu, Mn, Zn, and Fe in different tissues of transgenic tobacco was disordered compared with that of the wild type. Comparative transcriptome analysis showed that the overexpression of miR156 resulted in 2656 significantly differentially expressed genes. The expression levels of several metal-transport-related genes, such as NtABC, NtZIP, NtHMA, and NtCAX, were significantly increased or decreased in transgenic tobacco. These results suggest that miR156 plays an essential role in regulating mineral homeostasis. Our study provides a new perspective for the further study of mineral nutrient homeostasis in plants.</description><subject>Genetic aspects</subject><subject>Homeostasis</subject><subject>MicroRNA</subject><subject>Nutritional aspects</subject><subject>Physiological aspects</subject><subject>Tobacco (Plant)</subject><issn>2223-7747</issn><issn>2223-7747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNptTk1PAjEQbYwmEuTquYknDwvbdtmPIyEomyAkiGcydKdYs9vdtIXgL_BvW6MHSJzvvLx5M4Tcs3goRBGPuhqMd4zHBctEcUV6nHMRZVmSXZ_Nt2Tg3EccLA_B0h75mrZNBxa8PiLdWDBOWt35tkE6MVB_Ou3oGo8ItaP-HelMKZSetoo2es3GKdDVES2eOovO6dbQEC_aoIWaLg_eajSezoNc6zz8iGlDy6WWrddggHrYgTw0o_KO3KhwAwd_vU_enmab6TxarJ7L6WQR7VnMRDTOgFWyGAtVcZ6rKk0xC4BMVZ6rnQw1j1mV8ERwnjAmBZcqzRPIUImd4pXok4df3T3UuNVGtd6CbLST20mWFFwIEbJPhv-wglfYhN8NKh3wi4XHi4XA8Xjyezg4ty1f1-fcb82phB8</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Liu, Wanhong</creator><creator>Ji, Xue</creator><creator>Cao, Hanping</creator><creator>Huo, Chunsong</creator><creator>He, Linshen</creator><creator>Peng, Xiang</creator><creator>Yang, Ya</creator><creator>Yang, Fang</creator><creator>Xiong, Shu</creator><general>MDPI AG</general><scope>ISR</scope></search><sort><creationdate>20230401</creationdate><title>Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in INicotiana tabacum/I</title><author>Liu, Wanhong ; Ji, Xue ; Cao, Hanping ; Huo, Chunsong ; He, Linshen ; Peng, Xiang ; Yang, Ya ; Yang, Fang ; Xiong, Shu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1013-57a1dc953fd228fd66e71dcc6f88fbcf88801d424322411c32cf684a7ef3bf2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Genetic aspects</topic><topic>Homeostasis</topic><topic>MicroRNA</topic><topic>Nutritional aspects</topic><topic>Physiological aspects</topic><topic>Tobacco (Plant)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Wanhong</creatorcontrib><creatorcontrib>Ji, Xue</creatorcontrib><creatorcontrib>Cao, Hanping</creatorcontrib><creatorcontrib>Huo, Chunsong</creatorcontrib><creatorcontrib>He, Linshen</creatorcontrib><creatorcontrib>Peng, Xiang</creatorcontrib><creatorcontrib>Yang, Ya</creatorcontrib><creatorcontrib>Yang, Fang</creatorcontrib><creatorcontrib>Xiong, Shu</creatorcontrib><collection>Gale In Context: Science</collection><jtitle>Plants (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Wanhong</au><au>Ji, Xue</au><au>Cao, Hanping</au><au>Huo, Chunsong</au><au>He, Linshen</au><au>Peng, Xiang</au><au>Yang, Ya</au><au>Yang, Fang</au><au>Xiong, Shu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in INicotiana tabacum/I</atitle><jtitle>Plants (Basel)</jtitle><date>2023-04-01</date><risdate>2023</risdate><volume>12</volume><issue>9</issue><issn>2223-7747</issn><eissn>2223-7747</eissn><abstract>Mineral nutrition plays an important role in crop growth, yield and quality. MiR156 is a regulatory hub for growth and development. To date, the understanding of miR156-mediated mineral homeostasis is limited. In this study, we overexpressed Nta-miR156a in the tobacco cultivar TN90 and analyzed the effects of miR156 on mineral element homeostasis in tobacco by comparative transcriptome analysis. The results showed that the overexpression of miR156a caused significant morphological changes in transgenic tobacco. Chlorophyll and three anti-resistance markers, proline, total phenolics, and total flavonoids, were altered due to increased miR156 expression levels. Interestingly, the distribution of Cu, Mn, Zn, and Fe in different tissues of transgenic tobacco was disordered compared with that of the wild type. Comparative transcriptome analysis showed that the overexpression of miR156 resulted in 2656 significantly differentially expressed genes. The expression levels of several metal-transport-related genes, such as NtABC, NtZIP, NtHMA, and NtCAX, were significantly increased or decreased in transgenic tobacco. These results suggest that miR156 plays an essential role in regulating mineral homeostasis. Our study provides a new perspective for the further study of mineral nutrient homeostasis in plants.</abstract><pub>MDPI AG</pub><doi>10.3390/plants12091739</doi></addata></record> |
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| ispartof | Plants (Basel), 2023-04, Vol.12 (9) |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Genetic aspects Homeostasis MicroRNA Nutritional aspects Physiological aspects Tobacco (Plant) |
| title | Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in INicotiana tabacum/I |
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