Ex Vivo Antiplatelet and Thrombolytic Activity of Bioactive Fractions from the New-Fangled Stem Buds of IFicus religiosa/I L. with Simultaneous GC-MS Examination

Different parts of Ficus religiosa are the common components of various traditional formulations for the treatment of several blood disorders. The new-fangled stem buds' powder was extracted with 80% ethanol and successively fractionated by chloroform and methanol. Chloroform and methanol fract...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecules (Basel, Switzerland) Switzerland), 2023-05, Vol.28 (9)
Hauptverfasser: Kumar, Sunil, Arif, Muhammad, Kamal, Mehnaz, Jawaid, Talha, Khan, Mohammed Moizuddin, Mukhtar, Beenish, Khan, Abdullah, Ahmed, Saif, AlSanad, Saud M, Al-Khamees, Osama A
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 9
container_start_page
container_title Molecules (Basel, Switzerland)
container_volume 28
creator Kumar, Sunil
Arif, Muhammad
Kamal, Mehnaz
Jawaid, Talha
Khan, Mohammed Moizuddin
Mukhtar, Beenish
Khan, Abdullah
Ahmed, Saif
AlSanad, Saud M
Al-Khamees, Osama A
description Different parts of Ficus religiosa are the common components of various traditional formulations for the treatment of several blood disorders. The new-fangled stem buds' powder was extracted with 80% ethanol and successively fractionated by chloroform and methanol. Chloroform and methanol fractions of Ficus religiosa (CFFR and MFFR) were tested for antiplatelet, antithrombotic, thrombolytic, and antioxidant activity in ex vivo mode. The MFFR was particularly investigated for GC-MS and toxicity. The antiplatelet activity of the CFFR, MFFR, and standard drug aspirin at 50 μg/mL was 54.32%, 86.61%, and 87.57%, and a significant delay in clot formation was noted. CFFR at different concentrations did not show a significant effect on the delay of clot formation, antiplatelet, and free radical scavenging activity. The most possible marker compounds for antiplatelet and antioxidant activity identified by GC-MS in the MFFR are salicylate derivatives aromatic compounds such as benzeneacetaldehyde (7), phenylmalonic acid (13), and Salicylic acid (14), as well as Benzamides derivatives such as carbobenzyloxy-dl-norvaline (17), 3-acetoxy-2(1H)-pyridone (16), and 3-benzylhexahydropyrrolo [1,2-a] pyrazine-1,4-dione (35). A toxicity study of MFFR did not show any physical indications of toxicity and mortality up to 1500 mg/kg body weight and nontoxic up to 1000 mg/kg, which is promising for the treatment of atherothrombotic diseases.
doi_str_mv 10.3390/molecules28093918
format Article
fullrecord <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A749098693</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A749098693</galeid><sourcerecordid>A749098693</sourcerecordid><originalsourceid>FETCH-LOGICAL-g673-d0343b5cf8a4c723b25a686c14c864166034083fd6aa220e51ca82c5b01e0f2a3</originalsourceid><addsrcrecordid>eNptj89OwkAQxhujiYg-gLdJPBf2T1vaYyEUSVAPEK9ku52WNdtdwy4Ij-Ob2kYPHMwc5puZ3_clEwSPlIw4z8i4tRrlQaNjKcl4RtOrYEAjRkJOouz6Qt8Gd859EMJoRONB8D0_wbs6WsiNV59aeNToQZgKNru9bUurz15JyKVXR-XPYGuYKiv6EaHY98IaB3XHgt8hvOJXWAjTaKxg7bGF6aFyvWtZKHlwsEetGmWdGC9hNYIv5XewVu1Be2HQdsBiFr6sYX4SrTKiD78PbmqhHT789WGwKeab2XO4elssZ_kqbJIJDyvCI17Gsk5FJCeMlywWSZpIGsk0iWiSdHeS8rpKhGCMYEylSJmMS0KR1EzwYfD0G9sIjVtlauu771rl5DafRBnJ0iTjHTX6h-qqwlZJa7BW3f7C8AP8Q343</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Ex Vivo Antiplatelet and Thrombolytic Activity of Bioactive Fractions from the New-Fangled Stem Buds of IFicus religiosa/I L. with Simultaneous GC-MS Examination</title><source>DOAJ Directory of Open Access Journals</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Kumar, Sunil ; Arif, Muhammad ; Kamal, Mehnaz ; Jawaid, Talha ; Khan, Mohammed Moizuddin ; Mukhtar, Beenish ; Khan, Abdullah ; Ahmed, Saif ; AlSanad, Saud M ; Al-Khamees, Osama A</creator><creatorcontrib>Kumar, Sunil ; Arif, Muhammad ; Kamal, Mehnaz ; Jawaid, Talha ; Khan, Mohammed Moizuddin ; Mukhtar, Beenish ; Khan, Abdullah ; Ahmed, Saif ; AlSanad, Saud M ; Al-Khamees, Osama A</creatorcontrib><description>Different parts of Ficus religiosa are the common components of various traditional formulations for the treatment of several blood disorders. The new-fangled stem buds' powder was extracted with 80% ethanol and successively fractionated by chloroform and methanol. Chloroform and methanol fractions of Ficus religiosa (CFFR and MFFR) were tested for antiplatelet, antithrombotic, thrombolytic, and antioxidant activity in ex vivo mode. The MFFR was particularly investigated for GC-MS and toxicity. The antiplatelet activity of the CFFR, MFFR, and standard drug aspirin at 50 μg/mL was 54.32%, 86.61%, and 87.57%, and a significant delay in clot formation was noted. CFFR at different concentrations did not show a significant effect on the delay of clot formation, antiplatelet, and free radical scavenging activity. The most possible marker compounds for antiplatelet and antioxidant activity identified by GC-MS in the MFFR are salicylate derivatives aromatic compounds such as benzeneacetaldehyde (7), phenylmalonic acid (13), and Salicylic acid (14), as well as Benzamides derivatives such as carbobenzyloxy-dl-norvaline (17), 3-acetoxy-2(1H)-pyridone (16), and 3-benzylhexahydropyrrolo [1,2-a] pyrazine-1,4-dione (35). A toxicity study of MFFR did not show any physical indications of toxicity and mortality up to 1500 mg/kg body weight and nontoxic up to 1000 mg/kg, which is promising for the treatment of atherothrombotic diseases.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules28093918</identifier><language>eng</language><publisher>MDPI AG</publisher><subject>Aggregation ; Antioxidants ; Apixaban ; Aromatic compounds ; Blood platelets ; Methanol</subject><ispartof>Molecules (Basel, Switzerland), 2023-05, Vol.28 (9)</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Kumar, Sunil</creatorcontrib><creatorcontrib>Arif, Muhammad</creatorcontrib><creatorcontrib>Kamal, Mehnaz</creatorcontrib><creatorcontrib>Jawaid, Talha</creatorcontrib><creatorcontrib>Khan, Mohammed Moizuddin</creatorcontrib><creatorcontrib>Mukhtar, Beenish</creatorcontrib><creatorcontrib>Khan, Abdullah</creatorcontrib><creatorcontrib>Ahmed, Saif</creatorcontrib><creatorcontrib>AlSanad, Saud M</creatorcontrib><creatorcontrib>Al-Khamees, Osama A</creatorcontrib><title>Ex Vivo Antiplatelet and Thrombolytic Activity of Bioactive Fractions from the New-Fangled Stem Buds of IFicus religiosa/I L. with Simultaneous GC-MS Examination</title><title>Molecules (Basel, Switzerland)</title><description>Different parts of Ficus religiosa are the common components of various traditional formulations for the treatment of several blood disorders. The new-fangled stem buds' powder was extracted with 80% ethanol and successively fractionated by chloroform and methanol. Chloroform and methanol fractions of Ficus religiosa (CFFR and MFFR) were tested for antiplatelet, antithrombotic, thrombolytic, and antioxidant activity in ex vivo mode. The MFFR was particularly investigated for GC-MS and toxicity. The antiplatelet activity of the CFFR, MFFR, and standard drug aspirin at 50 μg/mL was 54.32%, 86.61%, and 87.57%, and a significant delay in clot formation was noted. CFFR at different concentrations did not show a significant effect on the delay of clot formation, antiplatelet, and free radical scavenging activity. The most possible marker compounds for antiplatelet and antioxidant activity identified by GC-MS in the MFFR are salicylate derivatives aromatic compounds such as benzeneacetaldehyde (7), phenylmalonic acid (13), and Salicylic acid (14), as well as Benzamides derivatives such as carbobenzyloxy-dl-norvaline (17), 3-acetoxy-2(1H)-pyridone (16), and 3-benzylhexahydropyrrolo [1,2-a] pyrazine-1,4-dione (35). A toxicity study of MFFR did not show any physical indications of toxicity and mortality up to 1500 mg/kg body weight and nontoxic up to 1000 mg/kg, which is promising for the treatment of atherothrombotic diseases.</description><subject>Aggregation</subject><subject>Antioxidants</subject><subject>Apixaban</subject><subject>Aromatic compounds</subject><subject>Blood platelets</subject><subject>Methanol</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptj89OwkAQxhujiYg-gLdJPBf2T1vaYyEUSVAPEK9ku52WNdtdwy4Ij-Ob2kYPHMwc5puZ3_clEwSPlIw4z8i4tRrlQaNjKcl4RtOrYEAjRkJOouz6Qt8Gd859EMJoRONB8D0_wbs6WsiNV59aeNToQZgKNru9bUurz15JyKVXR-XPYGuYKiv6EaHY98IaB3XHgt8hvOJXWAjTaKxg7bGF6aFyvWtZKHlwsEetGmWdGC9hNYIv5XewVu1Be2HQdsBiFr6sYX4SrTKiD78PbmqhHT789WGwKeab2XO4elssZ_kqbJIJDyvCI17Gsk5FJCeMlywWSZpIGsk0iWiSdHeS8rpKhGCMYEylSJmMS0KR1EzwYfD0G9sIjVtlauu771rl5DafRBnJ0iTjHTX6h-qqwlZJa7BW3f7C8AP8Q343</recordid><startdate>20230501</startdate><enddate>20230501</enddate><creator>Kumar, Sunil</creator><creator>Arif, Muhammad</creator><creator>Kamal, Mehnaz</creator><creator>Jawaid, Talha</creator><creator>Khan, Mohammed Moizuddin</creator><creator>Mukhtar, Beenish</creator><creator>Khan, Abdullah</creator><creator>Ahmed, Saif</creator><creator>AlSanad, Saud M</creator><creator>Al-Khamees, Osama A</creator><general>MDPI AG</general><scope/></search><sort><creationdate>20230501</creationdate><title>Ex Vivo Antiplatelet and Thrombolytic Activity of Bioactive Fractions from the New-Fangled Stem Buds of IFicus religiosa/I L. with Simultaneous GC-MS Examination</title><author>Kumar, Sunil ; Arif, Muhammad ; Kamal, Mehnaz ; Jawaid, Talha ; Khan, Mohammed Moizuddin ; Mukhtar, Beenish ; Khan, Abdullah ; Ahmed, Saif ; AlSanad, Saud M ; Al-Khamees, Osama A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g673-d0343b5cf8a4c723b25a686c14c864166034083fd6aa220e51ca82c5b01e0f2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aggregation</topic><topic>Antioxidants</topic><topic>Apixaban</topic><topic>Aromatic compounds</topic><topic>Blood platelets</topic><topic>Methanol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Sunil</creatorcontrib><creatorcontrib>Arif, Muhammad</creatorcontrib><creatorcontrib>Kamal, Mehnaz</creatorcontrib><creatorcontrib>Jawaid, Talha</creatorcontrib><creatorcontrib>Khan, Mohammed Moizuddin</creatorcontrib><creatorcontrib>Mukhtar, Beenish</creatorcontrib><creatorcontrib>Khan, Abdullah</creatorcontrib><creatorcontrib>Ahmed, Saif</creatorcontrib><creatorcontrib>AlSanad, Saud M</creatorcontrib><creatorcontrib>Al-Khamees, Osama A</creatorcontrib><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Sunil</au><au>Arif, Muhammad</au><au>Kamal, Mehnaz</au><au>Jawaid, Talha</au><au>Khan, Mohammed Moizuddin</au><au>Mukhtar, Beenish</au><au>Khan, Abdullah</au><au>Ahmed, Saif</au><au>AlSanad, Saud M</au><au>Al-Khamees, Osama A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ex Vivo Antiplatelet and Thrombolytic Activity of Bioactive Fractions from the New-Fangled Stem Buds of IFicus religiosa/I L. with Simultaneous GC-MS Examination</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><date>2023-05-01</date><risdate>2023</risdate><volume>28</volume><issue>9</issue><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>Different parts of Ficus religiosa are the common components of various traditional formulations for the treatment of several blood disorders. The new-fangled stem buds' powder was extracted with 80% ethanol and successively fractionated by chloroform and methanol. Chloroform and methanol fractions of Ficus religiosa (CFFR and MFFR) were tested for antiplatelet, antithrombotic, thrombolytic, and antioxidant activity in ex vivo mode. The MFFR was particularly investigated for GC-MS and toxicity. The antiplatelet activity of the CFFR, MFFR, and standard drug aspirin at 50 μg/mL was 54.32%, 86.61%, and 87.57%, and a significant delay in clot formation was noted. CFFR at different concentrations did not show a significant effect on the delay of clot formation, antiplatelet, and free radical scavenging activity. The most possible marker compounds for antiplatelet and antioxidant activity identified by GC-MS in the MFFR are salicylate derivatives aromatic compounds such as benzeneacetaldehyde (7), phenylmalonic acid (13), and Salicylic acid (14), as well as Benzamides derivatives such as carbobenzyloxy-dl-norvaline (17), 3-acetoxy-2(1H)-pyridone (16), and 3-benzylhexahydropyrrolo [1,2-a] pyrazine-1,4-dione (35). A toxicity study of MFFR did not show any physical indications of toxicity and mortality up to 1500 mg/kg body weight and nontoxic up to 1000 mg/kg, which is promising for the treatment of atherothrombotic diseases.</abstract><pub>MDPI AG</pub><doi>10.3390/molecules28093918</doi></addata></record>
fulltext fulltext
identifier ISSN: 1420-3049
ispartof Molecules (Basel, Switzerland), 2023-05, Vol.28 (9)
issn 1420-3049
1420-3049
language eng
recordid cdi_gale_infotracmisc_A749098693
source DOAJ Directory of Open Access Journals; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry
subjects Aggregation
Antioxidants
Apixaban
Aromatic compounds
Blood platelets
Methanol
title Ex Vivo Antiplatelet and Thrombolytic Activity of Bioactive Fractions from the New-Fangled Stem Buds of IFicus religiosa/I L. with Simultaneous GC-MS Examination
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T02%3A06%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ex%20Vivo%20Antiplatelet%20and%20Thrombolytic%20Activity%20of%20Bioactive%20Fractions%20from%20the%20New-Fangled%20Stem%20Buds%20of%20IFicus%20religiosa/I%20L.%20with%20Simultaneous%20GC-MS%20Examination&rft.jtitle=Molecules%20(Basel,%20Switzerland)&rft.au=Kumar,%20Sunil&rft.date=2023-05-01&rft.volume=28&rft.issue=9&rft.issn=1420-3049&rft.eissn=1420-3049&rft_id=info:doi/10.3390/molecules28093918&rft_dat=%3Cgale%3EA749098693%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A749098693&rfr_iscdi=true