Preserving Effect of Loboob, a Traditional Multi-herbal Formulation, on Sperm Parameters of Male Rats with Busulfan-induced Subfertility

Background: Male infertility caused by genotoxic substances during reproductive age is a serious concern. The purpose of this experimental study was to see how loboob affects sperm parameters. Methods: 55 healthy rats were selected, weighed, and divided into five groups (n=11 for each group). The control group received no treatment. the first experimental group received 10mg/Kg busulfan, and the rest of the experimental groups received 10mg/Kg busulfan and 35,70, and 140 mg/Kg loboob, respectively. Finally, sperm parameters and weights of the rats were compared using Kolmogorov--Smirnov, non-parametric Kruskal-Wallis, and Dunn-Bonferroni tests. Results: All sperm parameters and weights were significantly decreased among rats receiving Busulfan. All loboob dosages were beneficial in increasing slow sperm motility, but only 70 and 140 mg/Kg of loboob could increase progressive sperm percentages (0.024 and 0.01, respectively). Loboob at a dose of 140 mg/Kg has the potential to improve sperm viability. It did not improve normal morphology or count, nor did it reduce immotile sperm. Loboob had no effect on mean rat weights. Conclusion: Loboob has dose-dependent protective effects on several sperm parameters in busulfan-induced subfertile rats. Keywords * Busulfan * Infertility * Infertility, Male * Rats * Spermatozoa * Medicine, traditional