Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene during Abiotic and Hormonal Stresses
Pathogenesis-related protein-1 (PR-1) plays crucial roles in regulating plant responses to biotic and abiotic stresses. This study aimed to isolate and characterize the first PR-1 (AvPR-1) gene in oat (Avena sativa L.). AvPR-1 presented conserved signal peptide motifs and core amino acid composition...
Gespeichert in:
Veröffentlicht in: | Plants (Basel) 2022-08, Vol.11 (17) |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 17 |
container_start_page | |
container_title | Plants (Basel) |
container_volume | 11 |
creator | AlHudaib, Khalid A Alanazi, Naimah Asid Ghorbel, Mouna El-Ganainy, Sherif Mohamed Brini, Faiçal |
description | Pathogenesis-related protein-1 (PR-1) plays crucial roles in regulating plant responses to biotic and abiotic stresses. This study aimed to isolate and characterize the first PR-1 (AvPR-1) gene in oat (Avena sativa L.). AvPR-1 presented conserved signal peptide motifs and core amino acid composition in the functional protein domains as the protein sequence of AvPR-1 presented 98.28%, 97.7%, and 95.4% identity with known PR1 proteins isolated from Triticum aestivum PRB1-2-like, Triticum dicoccoides PRB1-2-like, and Aegilops tauschii subsp. tauschii, respectively. Bioinformatic analysis showed that the AvPR-1 protein belongs to the CAP superfamily (PF00188). Secondary and 3D structure analyses of the AvPR-1 protein were also conducted, confirming sequence conservation of PR-1 among studied species. The AvPR-1 protein harbors a calmodulin-binding domain located in its C-terminal part as previously shown for its wheat homolog TdPR1.2. Moreover, gene expression analysis showed that AvPR-1 was induced in response to many abiotic and hormonal stresses especially in leaves after treatment for 48 h. This is the first study exhibiting the expression profiles of the AvPR-1 gene under different stresses in oat. |
doi_str_mv | 10.3390/plants11172284 |
format | Article |
fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A746374986</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A746374986</galeid><sourcerecordid>A746374986</sourcerecordid><originalsourceid>FETCH-LOGICAL-g1016-71dff3ca247ffd4ddb02f5bd2123921959aed1e85921dcfec03bee658df0f2da3</originalsourceid><addsrcrecordid>eNptT8FKAzEQDaJgqb16DnjysHWT7G52j6VoWyhaWj2XbDLZRraJJKmIX2-wHlpwZmBm3rz3YBC6JfmYsSZ_-OiFjYEQwimtiws0oJSyjPOCX57M12gUwnueok5FqgHyi-B6EY2zWFiFpzvhhYzgzfcRdBoL_Ow-occrEXeuAwvBhGwNSQUKr7yLYGxG8CxdsDp4Yzs8aY2LRv5azp3fOyt6vIkeQoBwg6606AOM_voQvT09vk7n2fJltphOlllHclJlnCitmRS04FqrQqk2p7psFSWUNZQ0ZSNAEajLtCipQeasBajKWulcUyXYEN0dfTvRw9ZY7WL6bW-C3E54UTFeNHWVWON_WCkV7I10FrRJ-Jng_kyQOBG-YicOIWwXm_Up9wfp7X0m</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene during Abiotic and Hormonal Stresses</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>AlHudaib, Khalid A ; Alanazi, Naimah Asid ; Ghorbel, Mouna ; El-Ganainy, Sherif Mohamed ; Brini, Faiçal</creator><creatorcontrib>AlHudaib, Khalid A ; Alanazi, Naimah Asid ; Ghorbel, Mouna ; El-Ganainy, Sherif Mohamed ; Brini, Faiçal</creatorcontrib><description>Pathogenesis-related protein-1 (PR-1) plays crucial roles in regulating plant responses to biotic and abiotic stresses. This study aimed to isolate and characterize the first PR-1 (AvPR-1) gene in oat (Avena sativa L.). AvPR-1 presented conserved signal peptide motifs and core amino acid composition in the functional protein domains as the protein sequence of AvPR-1 presented 98.28%, 97.7%, and 95.4% identity with known PR1 proteins isolated from Triticum aestivum PRB1-2-like, Triticum dicoccoides PRB1-2-like, and Aegilops tauschii subsp. tauschii, respectively. Bioinformatic analysis showed that the AvPR-1 protein belongs to the CAP superfamily (PF00188). Secondary and 3D structure analyses of the AvPR-1 protein were also conducted, confirming sequence conservation of PR-1 among studied species. The AvPR-1 protein harbors a calmodulin-binding domain located in its C-terminal part as previously shown for its wheat homolog TdPR1.2. Moreover, gene expression analysis showed that AvPR-1 was induced in response to many abiotic and hormonal stresses especially in leaves after treatment for 48 h. This is the first study exhibiting the expression profiles of the AvPR-1 gene under different stresses in oat.</description><identifier>ISSN: 2223-7747</identifier><identifier>EISSN: 2223-7747</identifier><identifier>DOI: 10.3390/plants11172284</identifier><language>eng</language><publisher>MDPI AG</publisher><subject>Environmental aspects ; Gene expression ; Genetic aspects ; Oats ; Physiological aspects ; Stress (Physiology)</subject><ispartof>Plants (Basel), 2022-08, Vol.11 (17)</ispartof><rights>COPYRIGHT 2022 MDPI AG</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>AlHudaib, Khalid A</creatorcontrib><creatorcontrib>Alanazi, Naimah Asid</creatorcontrib><creatorcontrib>Ghorbel, Mouna</creatorcontrib><creatorcontrib>El-Ganainy, Sherif Mohamed</creatorcontrib><creatorcontrib>Brini, Faiçal</creatorcontrib><title>Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene during Abiotic and Hormonal Stresses</title><title>Plants (Basel)</title><description>Pathogenesis-related protein-1 (PR-1) plays crucial roles in regulating plant responses to biotic and abiotic stresses. This study aimed to isolate and characterize the first PR-1 (AvPR-1) gene in oat (Avena sativa L.). AvPR-1 presented conserved signal peptide motifs and core amino acid composition in the functional protein domains as the protein sequence of AvPR-1 presented 98.28%, 97.7%, and 95.4% identity with known PR1 proteins isolated from Triticum aestivum PRB1-2-like, Triticum dicoccoides PRB1-2-like, and Aegilops tauschii subsp. tauschii, respectively. Bioinformatic analysis showed that the AvPR-1 protein belongs to the CAP superfamily (PF00188). Secondary and 3D structure analyses of the AvPR-1 protein were also conducted, confirming sequence conservation of PR-1 among studied species. The AvPR-1 protein harbors a calmodulin-binding domain located in its C-terminal part as previously shown for its wheat homolog TdPR1.2. Moreover, gene expression analysis showed that AvPR-1 was induced in response to many abiotic and hormonal stresses especially in leaves after treatment for 48 h. This is the first study exhibiting the expression profiles of the AvPR-1 gene under different stresses in oat.</description><subject>Environmental aspects</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Oats</subject><subject>Physiological aspects</subject><subject>Stress (Physiology)</subject><issn>2223-7747</issn><issn>2223-7747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNptT8FKAzEQDaJgqb16DnjysHWT7G52j6VoWyhaWj2XbDLZRraJJKmIX2-wHlpwZmBm3rz3YBC6JfmYsSZ_-OiFjYEQwimtiws0oJSyjPOCX57M12gUwnueok5FqgHyi-B6EY2zWFiFpzvhhYzgzfcRdBoL_Ow-occrEXeuAwvBhGwNSQUKr7yLYGxG8CxdsDp4Yzs8aY2LRv5azp3fOyt6vIkeQoBwg6606AOM_voQvT09vk7n2fJltphOlllHclJlnCitmRS04FqrQqk2p7psFSWUNZQ0ZSNAEajLtCipQeasBajKWulcUyXYEN0dfTvRw9ZY7WL6bW-C3E54UTFeNHWVWON_WCkV7I10FrRJ-Jng_kyQOBG-YicOIWwXm_Up9wfp7X0m</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>AlHudaib, Khalid A</creator><creator>Alanazi, Naimah Asid</creator><creator>Ghorbel, Mouna</creator><creator>El-Ganainy, Sherif Mohamed</creator><creator>Brini, Faiçal</creator><general>MDPI AG</general><scope>ISR</scope></search><sort><creationdate>20220801</creationdate><title>Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene during Abiotic and Hormonal Stresses</title><author>AlHudaib, Khalid A ; Alanazi, Naimah Asid ; Ghorbel, Mouna ; El-Ganainy, Sherif Mohamed ; Brini, Faiçal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1016-71dff3ca247ffd4ddb02f5bd2123921959aed1e85921dcfec03bee658df0f2da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Environmental aspects</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Oats</topic><topic>Physiological aspects</topic><topic>Stress (Physiology)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AlHudaib, Khalid A</creatorcontrib><creatorcontrib>Alanazi, Naimah Asid</creatorcontrib><creatorcontrib>Ghorbel, Mouna</creatorcontrib><creatorcontrib>El-Ganainy, Sherif Mohamed</creatorcontrib><creatorcontrib>Brini, Faiçal</creatorcontrib><collection>Gale In Context: Science</collection><jtitle>Plants (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AlHudaib, Khalid A</au><au>Alanazi, Naimah Asid</au><au>Ghorbel, Mouna</au><au>El-Ganainy, Sherif Mohamed</au><au>Brini, Faiçal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene during Abiotic and Hormonal Stresses</atitle><jtitle>Plants (Basel)</jtitle><date>2022-08-01</date><risdate>2022</risdate><volume>11</volume><issue>17</issue><issn>2223-7747</issn><eissn>2223-7747</eissn><abstract>Pathogenesis-related protein-1 (PR-1) plays crucial roles in regulating plant responses to biotic and abiotic stresses. This study aimed to isolate and characterize the first PR-1 (AvPR-1) gene in oat (Avena sativa L.). AvPR-1 presented conserved signal peptide motifs and core amino acid composition in the functional protein domains as the protein sequence of AvPR-1 presented 98.28%, 97.7%, and 95.4% identity with known PR1 proteins isolated from Triticum aestivum PRB1-2-like, Triticum dicoccoides PRB1-2-like, and Aegilops tauschii subsp. tauschii, respectively. Bioinformatic analysis showed that the AvPR-1 protein belongs to the CAP superfamily (PF00188). Secondary and 3D structure analyses of the AvPR-1 protein were also conducted, confirming sequence conservation of PR-1 among studied species. The AvPR-1 protein harbors a calmodulin-binding domain located in its C-terminal part as previously shown for its wheat homolog TdPR1.2. Moreover, gene expression analysis showed that AvPR-1 was induced in response to many abiotic and hormonal stresses especially in leaves after treatment for 48 h. This is the first study exhibiting the expression profiles of the AvPR-1 gene under different stresses in oat.</abstract><pub>MDPI AG</pub><doi>10.3390/plants11172284</doi></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2223-7747 |
ispartof | Plants (Basel), 2022-08, Vol.11 (17) |
issn | 2223-7747 2223-7747 |
language | eng |
recordid | cdi_gale_infotracmisc_A746374986 |
source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Environmental aspects Gene expression Genetic aspects Oats Physiological aspects Stress (Physiology) |
title | Isolation and Characterization of a Novel Pathogenesis-Related Protein-1 Gene during Abiotic and Hormonal Stresses |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T11%3A59%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20Characterization%20of%20a%20Novel%20Pathogenesis-Related%20Protein-1%20Gene%20during%20Abiotic%20and%20Hormonal%20Stresses&rft.jtitle=Plants%20(Basel)&rft.au=AlHudaib,%20Khalid%20A&rft.date=2022-08-01&rft.volume=11&rft.issue=17&rft.issn=2223-7747&rft.eissn=2223-7747&rft_id=info:doi/10.3390/plants11172284&rft_dat=%3Cgale%3EA746374986%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A746374986&rfr_iscdi=true |