Identification of Alkaline Salt Tolerance Genes in IBrassica napus/I L. by Transcriptome Analysis

Soil salt alkalization is one major abiotic factor reducing the productivity of crops, including rapeseed, an indispensable oil crop and vegetable. The mechanism studies of alkali salt tolerance can help breed highly resistant varieties. In the current study, rapeseed (B. napus) line 2205 exhibited...

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Veröffentlicht in:Genes 2022-08, Vol.13 (8)
Hauptverfasser: Xu, Yu, Tao, Shunxian, Zhu, Yunlin, Zhang, Qi, Li, Ping, Wang, Han, Zhang, Yan, Bakirov, Aldiyar, Cao, Hanming, Qin, Mengfan, Wang, Kai, Shi, Yiji, Liu, Xiang, Zheng, Lin, Xu, Aixia, Huang, Zhen
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container_issue 8
container_start_page
container_title Genes
container_volume 13
creator Xu, Yu
Tao, Shunxian
Zhu, Yunlin
Zhang, Qi
Li, Ping
Wang, Han
Zhang, Yan
Bakirov, Aldiyar
Cao, Hanming
Qin, Mengfan
Wang, Kai
Shi, Yiji
Liu, Xiang
Zheng, Lin
Xu, Aixia
Huang, Zhen
description Soil salt alkalization is one major abiotic factor reducing the productivity of crops, including rapeseed, an indispensable oil crop and vegetable. The mechanism studies of alkali salt tolerance can help breed highly resistant varieties. In the current study, rapeseed (B. napus) line 2205 exhibited more tolerance to alkaline salt than line 1423 did. In line 2205, the lesser plasma membrane damage index, the accumulated osmotic solute, and higher antioxidant enzyme activities contributed to alkaline tolerance. A more integrated mesophyll-cell structure was revealed under alkali salt stress by ultrastructure observation in line 2205, which also implied a lesser injury. Transcriptome analysis showed that more genes responded to alkaline salt in line 2205. The expression of specific-response genes in line 1423 was lower than in line 2205. However, most of the specific-response genes in line 2205 had higher expression, which was mainly enriched in carbohydrate metabolism, photosynthetic processes, ROS regulating, and response to salt stress. It can be seen that the tolerance to alkaline salt is attributed to the high expression of some genes in these pathways. Based on these, twelve cross-differentially expressed genes were proposed as candidates. They provide clues for further analysis of the resistance mechanism of rapeseed.
doi_str_mv 10.3390/genes13081493
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The mechanism studies of alkali salt tolerance can help breed highly resistant varieties. In the current study, rapeseed (B. napus) line 2205 exhibited more tolerance to alkaline salt than line 1423 did. In line 2205, the lesser plasma membrane damage index, the accumulated osmotic solute, and higher antioxidant enzyme activities contributed to alkaline tolerance. A more integrated mesophyll-cell structure was revealed under alkali salt stress by ultrastructure observation in line 2205, which also implied a lesser injury. Transcriptome analysis showed that more genes responded to alkaline salt in line 2205. The expression of specific-response genes in line 1423 was lower than in line 2205. However, most of the specific-response genes in line 2205 had higher expression, which was mainly enriched in carbohydrate metabolism, photosynthetic processes, ROS regulating, and response to salt stress. It can be seen that the tolerance to alkaline salt is attributed to the high expression of some genes in these pathways. Based on these, twelve cross-differentially expressed genes were proposed as candidates. They provide clues for further analysis of the resistance mechanism of rapeseed.</abstract><pub>MDPI AG</pub><doi>10.3390/genes13081493</doi></addata></record>
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subjects Analysis
DNA testing
Genetic aspects
Methods
Physiological aspects
Plant breeding
Rape (Plant)
RNA sequencing
title Identification of Alkaline Salt Tolerance Genes in IBrassica napus/I L. by Transcriptome Analysis
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