Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of IPseudomonas aeruginosa/I PAO1 and PA14
The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, an...
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| Veröffentlicht in: | Microorganisms (Basel) 2022-08, Vol.10 (9) |
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| creator | Saeki, Erika Kushikawa Ma Camargo, Larissa Ciappina de Anversa, Laís Tavares, Eliandro Reis Yamada-Ogatta, Sueli Fumie Lioni, Lucy Megumi Yamauchi Kobayashi, Renata Katsuko Takayama Nakazato, Gerson |
| description | The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine the effect of bio-AgNPs on the QS regulatory genes lasI, lasR, rhlI, rhlR, pqsA, and mvfR. Bio-AgNPs had an MIC value of 62.50 μM, for both strains. Phenotypic and genotypic assays were carried out using sub-MIC values. Experimental results showed that treatment with sub-MICs of bio-AgNPs reduced (p < 0.05) the motility and rhamnolipids and elastase production in P. aeruginosa PAO1. In PA14, bio-AgNPs stimulated swarming and twitching motilities as well as biofilm formation and elastase and pyocyanin production. Bio-AgNP treatment increased (p < 0.05) the expression of QS genes in PAO1 and PA14. Despite the different phenotypic behaviors in both strains, both showed an increase in the expression of QS genes. Demonstrating that the bio-AgNPs acted in the induction of regulation. The possible mechanism underlying the action of bio-AgNPs involves the induction of the rhl and/or pqs system of PAO1 and of the las and/or pqs system of PA14. These results suggest that exposure to low concentrations of bio-AgNPs may promote the expression of QS regulatory genes in P. aeruginosa, consequently inducing the production of virulence factors such as elastase, pyocyanin, and biofilms. |
| doi_str_mv | 10.3390/microorganisms10091755 |
| format | Article |
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This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine the effect of bio-AgNPs on the QS regulatory genes lasI, lasR, rhlI, rhlR, pqsA, and mvfR. Bio-AgNPs had an MIC value of 62.50 μM, for both strains. Phenotypic and genotypic assays were carried out using sub-MIC values. Experimental results showed that treatment with sub-MICs of bio-AgNPs reduced (p < 0.05) the motility and rhamnolipids and elastase production in P. aeruginosa PAO1. In PA14, bio-AgNPs stimulated swarming and twitching motilities as well as biofilm formation and elastase and pyocyanin production. Bio-AgNP treatment increased (p < 0.05) the expression of QS genes in PAO1 and PA14. Despite the different phenotypic behaviors in both strains, both showed an increase in the expression of QS genes. Demonstrating that the bio-AgNPs acted in the induction of regulation. The possible mechanism underlying the action of bio-AgNPs involves the induction of the rhl and/or pqs system of PAO1 and of the las and/or pqs system of PA14. These results suggest that exposure to low concentrations of bio-AgNPs may promote the expression of QS regulatory genes in P. aeruginosa, consequently inducing the production of virulence factors such as elastase, pyocyanin, and biofilms.</description><identifier>ISSN: 2076-2607</identifier><identifier>EISSN: 2076-2607</identifier><identifier>DOI: 10.3390/microorganisms10091755</identifier><language>eng</language><publisher>MDPI AG</publisher><subject>Antibacterial agents ; Chemical properties ; Drug therapy ; Health aspects ; Nanoparticles ; Pharmaceutical research ; Pseudomonas aeruginosa infections ; Quorum sensing ; Silver</subject><ispartof>Microorganisms (Basel), 2022-08, Vol.10 (9)</ispartof><rights>COPYRIGHT 2022 MDPI AG</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Saeki, Erika Kushikawa</creatorcontrib><creatorcontrib>Ma</creatorcontrib><creatorcontrib>Camargo, Larissa Ciappina de</creatorcontrib><creatorcontrib>Anversa, Laís</creatorcontrib><creatorcontrib>Tavares, Eliandro Reis</creatorcontrib><creatorcontrib>Yamada-Ogatta, Sueli Fumie</creatorcontrib><creatorcontrib>Lioni, Lucy Megumi Yamauchi</creatorcontrib><creatorcontrib>Kobayashi, Renata Katsuko Takayama</creatorcontrib><creatorcontrib>Nakazato, Gerson</creatorcontrib><title>Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of IPseudomonas aeruginosa/I PAO1 and PA14</title><title>Microorganisms (Basel)</title><description>The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine the effect of bio-AgNPs on the QS regulatory genes lasI, lasR, rhlI, rhlR, pqsA, and mvfR. Bio-AgNPs had an MIC value of 62.50 μM, for both strains. Phenotypic and genotypic assays were carried out using sub-MIC values. Experimental results showed that treatment with sub-MICs of bio-AgNPs reduced (p < 0.05) the motility and rhamnolipids and elastase production in P. aeruginosa PAO1. In PA14, bio-AgNPs stimulated swarming and twitching motilities as well as biofilm formation and elastase and pyocyanin production. Bio-AgNP treatment increased (p < 0.05) the expression of QS genes in PAO1 and PA14. Despite the different phenotypic behaviors in both strains, both showed an increase in the expression of QS genes. Demonstrating that the bio-AgNPs acted in the induction of regulation. The possible mechanism underlying the action of bio-AgNPs involves the induction of the rhl and/or pqs system of PAO1 and of the las and/or pqs system of PA14. These results suggest that exposure to low concentrations of bio-AgNPs may promote the expression of QS regulatory genes in P. aeruginosa, consequently inducing the production of virulence factors such as elastase, pyocyanin, and biofilms.</description><subject>Antibacterial agents</subject><subject>Chemical properties</subject><subject>Drug therapy</subject><subject>Health aspects</subject><subject>Nanoparticles</subject><subject>Pharmaceutical research</subject><subject>Pseudomonas aeruginosa infections</subject><subject>Quorum sensing</subject><subject>Silver</subject><issn>2076-2607</issn><issn>2076-2607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptkE9LAzEUxIMoWGq_ggQ8b5ts_u0ea6laKLbS3ks2-7JGdhNJdgW_vVv00IPM4Q3Db95hELqnZM5YSRadMzGE2GjvUpcoISVVQlyhSU6UzHJJ1PWFv0WzlD4IOWOsEHSCwtpaMD0OFj-60IB3Bh9c-wURv2ofPnXsnWkh4eBx_w74bQhx6LID-OR8gw_fqYfu3N7sEwx16ILXCWuIQ-N8SHqxwfvljmLt69FQfodurG4TzP7uFB2f1sfVS7bdPW9Wy23WSMUyQyyRuRbSCkYqSmVtoOZKVrksQFWFVUblitLSlNSMIEAFpeIkV0RURHA2RQ-_bxvdwsl5G_qoTeeSOS0V50VZFJyN1PwfalQN467Bg3VjflH4AcLYbnk</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Saeki, Erika Kushikawa</creator><creator>Ma</creator><creator>Camargo, Larissa Ciappina de</creator><creator>Anversa, Laís</creator><creator>Tavares, Eliandro Reis</creator><creator>Yamada-Ogatta, Sueli Fumie</creator><creator>Lioni, Lucy Megumi Yamauchi</creator><creator>Kobayashi, Renata Katsuko Takayama</creator><creator>Nakazato, Gerson</creator><general>MDPI AG</general><scope/></search><sort><creationdate>20220801</creationdate><title>Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of IPseudomonas aeruginosa/I PAO1 and PA14</title><author>Saeki, Erika Kushikawa ; Ma ; Camargo, Larissa Ciappina de ; Anversa, Laís ; Tavares, Eliandro Reis ; Yamada-Ogatta, Sueli Fumie ; Lioni, Lucy Megumi Yamauchi ; Kobayashi, Renata Katsuko Takayama ; Nakazato, Gerson</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g673-c0f062a56f530b116dced476b268e7b8f7c727119c91c62aeebe97402705b0543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antibacterial agents</topic><topic>Chemical properties</topic><topic>Drug therapy</topic><topic>Health aspects</topic><topic>Nanoparticles</topic><topic>Pharmaceutical research</topic><topic>Pseudomonas aeruginosa infections</topic><topic>Quorum sensing</topic><topic>Silver</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saeki, Erika Kushikawa</creatorcontrib><creatorcontrib>Ma</creatorcontrib><creatorcontrib>Camargo, Larissa Ciappina de</creatorcontrib><creatorcontrib>Anversa, Laís</creatorcontrib><creatorcontrib>Tavares, Eliandro Reis</creatorcontrib><creatorcontrib>Yamada-Ogatta, Sueli Fumie</creatorcontrib><creatorcontrib>Lioni, Lucy Megumi Yamauchi</creatorcontrib><creatorcontrib>Kobayashi, Renata Katsuko Takayama</creatorcontrib><creatorcontrib>Nakazato, Gerson</creatorcontrib><jtitle>Microorganisms (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saeki, Erika Kushikawa</au><au>Ma</au><au>Camargo, Larissa Ciappina de</au><au>Anversa, Laís</au><au>Tavares, Eliandro Reis</au><au>Yamada-Ogatta, Sueli Fumie</au><au>Lioni, Lucy Megumi Yamauchi</au><au>Kobayashi, Renata Katsuko Takayama</au><au>Nakazato, Gerson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of IPseudomonas aeruginosa/I PAO1 and PA14</atitle><jtitle>Microorganisms (Basel)</jtitle><date>2022-08-01</date><risdate>2022</risdate><volume>10</volume><issue>9</issue><issn>2076-2607</issn><eissn>2076-2607</eissn><abstract>The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine the effect of bio-AgNPs on the QS regulatory genes lasI, lasR, rhlI, rhlR, pqsA, and mvfR. Bio-AgNPs had an MIC value of 62.50 μM, for both strains. Phenotypic and genotypic assays were carried out using sub-MIC values. Experimental results showed that treatment with sub-MICs of bio-AgNPs reduced (p < 0.05) the motility and rhamnolipids and elastase production in P. aeruginosa PAO1. In PA14, bio-AgNPs stimulated swarming and twitching motilities as well as biofilm formation and elastase and pyocyanin production. Bio-AgNP treatment increased (p < 0.05) the expression of QS genes in PAO1 and PA14. Despite the different phenotypic behaviors in both strains, both showed an increase in the expression of QS genes. Demonstrating that the bio-AgNPs acted in the induction of regulation. The possible mechanism underlying the action of bio-AgNPs involves the induction of the rhl and/or pqs system of PAO1 and of the las and/or pqs system of PA14. These results suggest that exposure to low concentrations of bio-AgNPs may promote the expression of QS regulatory genes in P. aeruginosa, consequently inducing the production of virulence factors such as elastase, pyocyanin, and biofilms.</abstract><pub>MDPI AG</pub><doi>10.3390/microorganisms10091755</doi></addata></record> |
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| subjects | Antibacterial agents Chemical properties Drug therapy Health aspects Nanoparticles Pharmaceutical research Pseudomonas aeruginosa infections Quorum sensing Silver |
| title | Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of IPseudomonas aeruginosa/I PAO1 and PA14 |
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