Persistence of Tembusu Virus in ICulex tritaeniorhynchus/I in Yunnan Province, China
The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a signifi...
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| Veröffentlicht in: | Pathogens (Basel) 2023-03, Vol.12 (3) |
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| creator | Hu, Danhe Wu, Chao Wang, Ruichen Yao, Xiaohui Nie, Kai Lv, Quan Fu, Shihong Yin, Qikai Su, Wenzhe Li, Fan Xu, Songtao He, Ying Liang, Guodong Li, Xiangdong Wang, Huanyu |
| description | The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken. |
| doi_str_mv | 10.3390/pathogens12030490 |
| format | Article |
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In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken.</description><identifier>ISSN: 2076-0817</identifier><identifier>EISSN: 2076-0817</identifier><identifier>DOI: 10.3390/pathogens12030490</identifier><language>eng</language><publisher>MDPI AG</publisher><subject>Disease transmission ; Diseases ; Distribution ; Health aspects ; Mosquitoes ; Poultry ; Risk factors</subject><ispartof>Pathogens (Basel), 2023-03, Vol.12 (3)</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,862,27907,27908</link.rule.ids></links><search><creatorcontrib>Hu, Danhe</creatorcontrib><creatorcontrib>Wu, Chao</creatorcontrib><creatorcontrib>Wang, Ruichen</creatorcontrib><creatorcontrib>Yao, Xiaohui</creatorcontrib><creatorcontrib>Nie, Kai</creatorcontrib><creatorcontrib>Lv, Quan</creatorcontrib><creatorcontrib>Fu, Shihong</creatorcontrib><creatorcontrib>Yin, Qikai</creatorcontrib><creatorcontrib>Su, Wenzhe</creatorcontrib><creatorcontrib>Li, Fan</creatorcontrib><creatorcontrib>Xu, Songtao</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Liang, Guodong</creatorcontrib><creatorcontrib>Li, Xiangdong</creatorcontrib><creatorcontrib>Wang, Huanyu</creatorcontrib><title>Persistence of Tembusu Virus in ICulex tritaeniorhynchus/I in Yunnan Province, China</title><title>Pathogens (Basel)</title><description>The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken.</description><subject>Disease transmission</subject><subject>Diseases</subject><subject>Distribution</subject><subject>Health aspects</subject><subject>Mosquitoes</subject><subject>Poultry</subject><subject>Risk factors</subject><issn>2076-0817</issn><issn>2076-0817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptj01LxDAQhoMouNT9Ad4CXu3upGma5rgUPwoL7qEInpa0nbSRNpWmFf33dtHDHpz3MF_vPDCE3DLYcK5g-6GndmjQeRYBh1jBBVlFIJMQUiYvz-prsvb-HZZI4dSvSHHA0Vs_oauQDoYW2Jezn-mrHWdPraN5Nnf4RafRThqdHcb221Xt7Lf5afs2O6cdPYzDp10I9zRrrdM35MrozuP6LwekeHwosudw__KUZ7t92CQyCmsmQLEoASYMw1poIWKWYhotLzGpleZGMeRQgomMSuNUx3EphOZohMCk5AG5-8U2usOjdWaYRl311lfHnYy5TJRYYAHZ_ONaVGNvq8Ghscv87OAHv7RjVQ</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Hu, Danhe</creator><creator>Wu, Chao</creator><creator>Wang, Ruichen</creator><creator>Yao, Xiaohui</creator><creator>Nie, Kai</creator><creator>Lv, Quan</creator><creator>Fu, Shihong</creator><creator>Yin, Qikai</creator><creator>Su, Wenzhe</creator><creator>Li, Fan</creator><creator>Xu, Songtao</creator><creator>He, Ying</creator><creator>Liang, Guodong</creator><creator>Li, Xiangdong</creator><creator>Wang, Huanyu</creator><general>MDPI AG</general><scope/></search><sort><creationdate>20230301</creationdate><title>Persistence of Tembusu Virus in ICulex tritaeniorhynchus/I in Yunnan Province, China</title><author>Hu, Danhe ; Wu, Chao ; Wang, Ruichen ; Yao, Xiaohui ; Nie, Kai ; Lv, Quan ; Fu, Shihong ; Yin, Qikai ; Su, Wenzhe ; Li, Fan ; Xu, Songtao ; He, Ying ; Liang, Guodong ; Li, Xiangdong ; Wang, Huanyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g672-d1509126015f1ed5a55418e8239017a9a3f91e30b0f2f9848a44b55a3ef55e6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Disease transmission</topic><topic>Diseases</topic><topic>Distribution</topic><topic>Health aspects</topic><topic>Mosquitoes</topic><topic>Poultry</topic><topic>Risk factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Danhe</creatorcontrib><creatorcontrib>Wu, Chao</creatorcontrib><creatorcontrib>Wang, Ruichen</creatorcontrib><creatorcontrib>Yao, Xiaohui</creatorcontrib><creatorcontrib>Nie, Kai</creatorcontrib><creatorcontrib>Lv, Quan</creatorcontrib><creatorcontrib>Fu, Shihong</creatorcontrib><creatorcontrib>Yin, Qikai</creatorcontrib><creatorcontrib>Su, Wenzhe</creatorcontrib><creatorcontrib>Li, Fan</creatorcontrib><creatorcontrib>Xu, Songtao</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Liang, Guodong</creatorcontrib><creatorcontrib>Li, Xiangdong</creatorcontrib><creatorcontrib>Wang, Huanyu</creatorcontrib><jtitle>Pathogens (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Danhe</au><au>Wu, Chao</au><au>Wang, Ruichen</au><au>Yao, Xiaohui</au><au>Nie, Kai</au><au>Lv, Quan</au><au>Fu, Shihong</au><au>Yin, Qikai</au><au>Su, Wenzhe</au><au>Li, Fan</au><au>Xu, Songtao</au><au>He, Ying</au><au>Liang, Guodong</au><au>Li, Xiangdong</au><au>Wang, Huanyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Persistence of Tembusu Virus in ICulex tritaeniorhynchus/I in Yunnan Province, China</atitle><jtitle>Pathogens (Basel)</jtitle><date>2023-03-01</date><risdate>2023</risdate><volume>12</volume><issue>3</issue><issn>2076-0817</issn><eissn>2076-0817</eissn><abstract>The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken.</abstract><pub>MDPI AG</pub><doi>10.3390/pathogens12030490</doi></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Disease transmission Diseases Distribution Health aspects Mosquitoes Poultry Risk factors |
| title | Persistence of Tembusu Virus in ICulex tritaeniorhynchus/I in Yunnan Province, China |
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