Expression and purification of soluble recombinant [beta]-lactamases using Escherichia coli as expression host and pET-28a as cloning vector

Due to its high expression capability, recombination of Escherichia coli and pET vector has become the bioengineering preferred expression system. Because [beta]-lactamases mediate bacterial antimicrobial resistance, these enzymes have a substantial clinical impact. Using the E. coli expression syst...

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Veröffentlicht in:	Microbial cell factories 2022-11, Vol.21 (1)
Hauptverfasser:	Li, Lele, Li, Hui, Tian, Qingwu, Ge, Baosheng, Xu, Xiaotong, Chi, Yuanyuan, Zhao, Huaizhi, Liu, Yanfei, Jia, Nan, Zhou, Tingting, Zhu, Yuanqi, Zhou, Yusun
Format:	Artikel
Sprache:	eng
Schlagworte:	Beta lactamases Chemical properties Escherichia coli Genetic aspects Genetic vectors Microbiological research Microbiological synthesis Physiological aspects Production processes Recombinant proteins
Online-Zugang:	Volltext
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