A New Biorecognition-Element-Free IDμE Sensor for the Identification and Quantification of IE. coli/I

The label-free biosensor has emerged as an effective tool for the purpose of early detection of causative pathogens such as Escherichia coli as a preventive measure. In this study, a biorecognition-element-free interdigitated microelectrode (IDμE) sensor is designed and developed with this in mind,...

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Veröffentlicht in:	Biosensors (Basel) 2022-07, Vol.12 (8)
Hauptverfasser:	Lin, Yung-Kai, Wu, Hsing-Ju, Hieu, Nguyen Van, Chu, Pei-Yi, Do, Thi Vien Thao, Yao, Fiona Yan-Dong, Phan, Thien Luan, Ching, Congo Tak Shing
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Schlagworte:	Biosensors Control Design and construction Escherichia coli Escherichia coli infections Food contamination Microbial contamination Testing
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container_title	Biosensors (Basel)
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creator	Lin, Yung-Kai Wu, Hsing-Ju Hieu, Nguyen Van Chu, Pei-Yi Do, Thi Vien Thao Yao, Fiona Yan-Dong Phan, Thien Luan Ching, Congo Tak Shing
description	The label-free biosensor has emerged as an effective tool for the purpose of early detection of causative pathogens such as Escherichia coli as a preventive measure. In this study, a biorecognition-element-free interdigitated microelectrode (IDμE) sensor is designed and developed with this in mind, with good reliability and affordability. Results show that the designed sensor can identify E. coli with good selectivity using an impedance and capacitance of 7.69 MHz. At its optimum impedance of 1.3 kHz, the IDμE sensor can reliably quantify E. coli in a range of measurement (10[sup.3.2]~10[sup.6] cfu/mL), linearity (R[sup.2] = 0.97), sensitivity (18.15 kΩ/log (cfu/mL)), and limit of detection (10[sup.3.2] cfu/mL). In summary, the IDμE sensor developed possesses high potential for industrial and clinical applications.
doi_str_mv	10.3390/bios12080561
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source	MDPI - Multidisciplinary Digital Publishing Institute; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access
subjects	Biosensors Control Design and construction Escherichia coli Escherichia coli infections Food contamination Microbial contamination Testing
title	A New Biorecognition-Element-Free IDμE Sensor for the Identification and Quantification of IE. coli/I
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